##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930988_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1935267 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.98865531216106 28.0 2.0 31.0 2.0 31.0 2 20.405961554658866 28.0 2.0 31.0 2.0 33.0 3 21.141228574661792 30.0 2.0 31.0 2.0 33.0 4 22.819074060581823 32.0 2.0 35.0 2.0 37.0 5 22.01084863225591 32.0 2.0 35.0 2.0 37.0 6 22.56229760544669 31.0 2.0 35.0 2.0 37.0 7 22.285752818603324 30.0 2.0 35.0 2.0 37.0 8 22.025548412699642 29.0 2.0 35.0 2.0 37.0 9 20.837392979883397 26.0 2.0 33.0 2.0 37.0 10-11 20.83302045660883 26.0 2.0 33.5 2.0 37.0 12-13 20.439418178473566 25.5 2.0 33.0 2.0 37.0 14-15 21.980911677820167 28.5 2.0 35.0 2.0 37.5 16-17 23.27072104262616 31.5 2.0 36.5 2.0 38.5 18-19 23.453758835344168 32.0 2.0 37.5 2.0 39.5 20-21 23.7385802579179 32.0 2.0 38.0 2.0 40.0 22-23 23.721697057821995 32.0 2.0 38.0 2.0 40.0 24-25 23.78375412798337 32.0 2.0 38.0 2.0 40.0 26-27 23.627862718684298 32.0 2.0 38.0 2.0 40.0 28-29 23.563361799689655 32.0 2.0 38.0 2.0 40.0 30-31 23.6923806895896 32.0 2.0 38.0 2.0 40.0 32-33 23.645129586770196 32.0 2.0 38.0 2.0 40.0 34-35 23.378395332530346 31.0 2.0 38.0 2.0 40.0 36-37 22.920850973018194 30.0 2.0 38.0 2.0 40.0 38-39 22.910960864831573 30.0 2.0 38.0 2.0 40.0 40-41 22.882663219080364 30.0 2.0 38.0 2.0 40.0 42-43 22.81925129710784 30.0 2.0 38.0 2.0 40.0 44-45 22.47805909985547 30.0 2.0 38.0 2.0 40.0 46-47 22.172699425970677 30.0 2.0 38.0 2.0 40.0 48-49 22.035914682573516 29.5 2.0 38.0 2.0 40.0 50-51 19.25234218327497 18.5 2.0 35.5 2.0 38.5 52-53 21.51929165329642 28.5 2.0 36.5 2.0 39.0 54-55 22.46744790253748 30.5 2.0 38.0 2.0 40.0 56-57 22.521035081980934 31.0 2.0 38.0 2.0 40.0 58-59 22.284272144360443 30.5 2.0 38.0 2.0 40.0 60-61 22.035183517313115 30.0 2.0 38.0 2.0 40.0 62-63 21.625645711935356 29.0 2.0 37.0 2.0 40.0 64-65 21.331189443110432 28.0 2.0 37.0 2.0 39.5 66-67 21.03671922272224 28.0 2.0 36.0 2.0 39.0 68-69 20.628662349949643 27.5 2.0 36.0 2.0 39.0 70-71 20.326243355567993 26.5 2.0 35.5 2.0 39.0 72-73 19.956816294599143 26.0 2.0 35.0 2.0 38.5 74-75 19.53929535304431 26.0 2.0 34.5 2.0 38.0 76-77 19.17339312870007 25.5 2.0 34.0 2.0 37.0 78-79 18.845450007673357 25.0 2.0 34.0 2.0 36.5 80-81 18.449478030680005 23.5 2.0 34.0 2.0 36.0 82-83 18.021751778953497 21.5 2.0 34.0 2.0 35.5 84-85 17.67375199391092 20.5 2.0 33.0 2.0 35.0 86-87 17.21326514635965 18.5 2.0 33.0 2.0 35.0 88-89 16.83593194117401 17.0 2.0 32.5 2.0 35.0 90-91 16.386443317640406 9.0 2.0 32.0 2.0 35.0 92-93 15.873756696104465 4.5 2.0 32.0 2.0 34.5 94-95 15.330119306534964 2.0 2.0 32.0 2.0 34.0 96-97 14.688476318771517 2.0 2.0 31.0 2.0 34.0 98-99 13.984567245759887 2.0 2.0 31.0 2.0 34.0 100-101 12.78845192937202 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.294462737814197 1101 2 10.361839865636586 1101 3 9.77316918531979 1101 4 11.897564497874825 1101 5 12.609607089163372 1101 6 12.05817665096691 1101 7 12.176835903943452 1101 8 12.240545340285497 1101 9 13.442088345425205 1101 10-11 12.720394649506982 1101 12-13 12.175935140594323 1101 14-15 11.722425546443578 1101 16-17 11.32411873950782 1101 18-19 11.787221168913465 1101 20-21 11.91735270482037 1101 22-23 12.10982188595025 1101 24-25 12.18413823729944 1101 26-27 12.358434414089519 1101 28-29 12.371400391778437 1101 30-31 11.90625523924649 1101 32-33 12.032891231974666 1101 34-35 12.06333950649173 1101 36-37 12.488360682672916 1101 38-39 12.145523698665613 1101 40-41 12.06839933329077 1101 42-43 11.681392613431157 1101 44-45 11.7584790585462 1101 46-47 11.620240836483177 1101 48-49 11.66452405050535 1101 50-51 13.56559502943118 1101 52-53 12.343545129837771 1101 54-55 11.979393258501872 1101 56-57 11.908901057265059 1101 58-59 11.856289953793677 1101 60-61 11.718444889952238 1101 62-63 11.973767778923484 1101 64-65 11.743932866644496 1101 66-67 11.488398042919716 1101 68-69 11.63255566514945 1101 70-71 11.285733771349108 1101 72-73 11.165903628088408 1101 74-75 11.100651959975398 1101 76-77 11.048095899031122 1101 78-79 10.652237892112023 1101 80-81 10.390366644282931 1101 82-83 10.10238651910094 1101 84-85 10.000752087611865 1101 86-87 9.87835413784407 1101 88-89 9.600126785232515 1101 90-91 9.3076195495585 1101 92-93 9.185000288723453 1101 94-95 8.973955400430443 1101 96-97 9.14667322191795 1101 98-99 9.10205377088341 1101 100-101 8.412083416001755 1102 1 9.155514591387242 1102 2 9.951793220409751 1102 3 9.365833005976683 1102 4 11.351199092961131 1102 5 12.061185042557693 1102 6 11.37237093933675 1102 7 11.423104903777837 1102 8 11.612825666480735 1102 9 11.940889461746586 1102 10-11 11.120373390951027 1102 12-13 10.767877529907697 1102 14-15 10.306228257156658 1102 16-17 10.291007673440678 1102 18-19 11.241355340064619 1102 20-21 11.55125886559932 1102 22-23 11.89477601937983 1102 24-25 12.085267783478702 1102 26-27 12.107498666838918 1102 28-29 12.058022456676355 1102 30-31 12.10706565989399 1102 32-33 11.876617238851566 1102 34-35 11.892076871864504 1102 36-37 12.199272501688444 1102 38-39 12.110117202940813 1102 40-41 11.797260665707839 1102 42-43 11.305758132053406 1102 44-45 11.356522777013708 1102 46-47 11.459333219740337 1102 48-49 11.13034820269683 1102 50-51 13.386196300495072 1102 52-53 12.1820936895706 1102 54-55 11.717377972116985 1102 56-57 11.565038600122062 1102 58-59 11.625295473602442 1102 60-61 11.589929132665624 1102 62-63 11.576996483906644 1102 64-65 11.688513294435854 1102 66-67 11.563532577443855 1102 68-69 11.110138641495599 1102 70-71 10.86535868143283 1102 72-73 11.044543846804338 1102 74-75 10.763468026499847 1102 76-77 10.56459896545422 1102 78-79 10.264956811979626 1102 80-81 9.962183855308705 1102 82-83 10.01300702174219 1102 84-85 10.03217853671616 1102 86-87 10.031110705131109 1102 88-89 10.000408708291374 1102 90-91 9.902465956491758 1102 92-93 9.689932026511526 1102 94-95 9.513670708815459 1102 96-97 9.316943131420771 1102 98-99 8.73698565698699 1102 100-101 8.302178535680008 1103 1 9.073587345969493 1103 2 9.920002860715819 1103 3 9.338440505058912 1103 4 11.516916237388166 1103 5 12.067446754624992 1103 6 11.264613681754046 1103 7 11.19864494855062 1103 8 11.070166028454093 1103 9 10.84111404551081 1103 10-11 10.117708858150042 1103 12-13 9.558688430858101 1103 14-15 9.568602558631564 1103 16-17 10.206359629310928 1103 18-19 11.273723235143688 1103 20-21 11.84286864651018 1103 22-23 12.190688162979068 1103 24-25 12.348594766402176 1103 26-27 12.329658078122325 1103 28-29 12.244843636652888 1103 30-31 12.011132281611914 1103 32-33 12.120051043026997 1103 34-35 12.141616067249984 1103 36-37 12.59543082364463 1103 38-39 12.371149880358388 1103 40-41 11.989893670403468 1103 42-43 11.77466119872551 1103 44-45 11.93148072754289 1103 46-47 11.939678181361948 1103 48-49 11.841619380393936 1103 50-51 13.832026024732123 1103 52-53 12.347832788500227 1103 54-55 11.955951553292259 1103 56-57 11.743121648126841 1103 58-59 11.723490165651555 1103 60-61 11.866805762757451 1103 62-63 11.863543172615788 1103 64-65 11.783013871491367 1103 66-67 11.715421624426494 1103 68-69 11.584240366423675 1103 70-71 11.331459969670256 1103 72-73 11.319883661714684 1103 74-75 11.307610013346338 1103 76-77 11.192068727025923 1103 78-79 10.701403085832656 1103 80-81 10.469647066520618 1103 82-83 10.23838942598817 1103 84-85 9.953266976170923 1103 86-87 9.99673835196834 1103 88-89 9.850788533183223 1103 90-91 9.501888301136658 1103 92-93 9.404948186465072 1103 94-95 9.245993855099629 1103 96-97 9.0379045226884 1103 98-99 8.468344964616358 1103 100-101 7.979794180722488 1104 1 8.661839460400177 1104 2 9.757145891903445 1104 3 9.261418254117405 1104 4 11.2754802042166 1104 5 11.580080067374407 1104 6 10.718363519013653 1104 7 10.76261321029212 1104 8 10.170879219563272 1104 9 9.849371104860591 1104 10-11 9.223378434833915 1104 12-13 8.655509230095578 1104 14-15 9.0061723578368 1104 16-17 9.829918466084411 1104 18-19 11.093454656760148 1104 20-21 11.716767834659574 1104 22-23 11.991486397141298 1104 24-25 11.939377150545955 1104 26-27 12.147022985679815 1104 28-29 12.079443617780974 1104 30-31 11.900550632517223 1104 32-33 11.766134941820987 1104 34-35 11.761277017797212 1104 36-37 12.135994807195008 1104 38-39 11.6610842228215 1104 40-41 11.483659608510756 1104 42-43 11.284832801751222 1104 44-45 11.355831945796574 1104 46-47 11.317435600830834 1104 48-49 11.128983559352228 1104 50-51 13.099504701261552 1104 52-53 11.730905757111934 1104 54-55 11.38737914187977 1104 56-57 11.016927671133853 1104 58-59 11.049559086357263 1104 60-61 11.032487188136344 1104 62-63 11.188615704067974 1104 64-65 11.132632431898802 1104 66-67 10.954000712674183 1104 68-69 10.874870461392398 1104 70-71 10.51952814266598 1104 72-73 10.43505464961158 1104 74-75 10.261230136061553 1104 76-77 9.855483253382697 1104 78-79 9.597170760633755 1104 80-81 9.452717765725009 1104 82-83 9.2074492822033 1104 84-85 9.001341264622148 1104 86-87 8.87446173032751 1104 88-89 8.592965109794232 1104 90-91 8.449529132790827 1104 92-93 8.20171738654677 1104 94-95 7.977234635632412 1104 96-97 7.68691273197075 1104 98-99 7.450026905986819 1104 100-101 6.800400040809945 1105 1 8.696746884622062 1105 2 9.471497375494554 1105 3 9.020207599317203 1105 4 10.851924482385016 1105 5 10.801426266069658 1105 6 10.525977638142262 1105 7 10.180275072289579 1105 8 9.732458870726198 1105 9 8.895974404240906 1105 10-11 8.499459194757712 1105 12-13 7.724029187210004 1105 14-15 8.465675672028492 1105 16-17 9.894792893712907 1105 18-19 10.870213554691137 1105 20-21 11.793085623173983 1105 22-23 12.098851699029549 1105 24-25 12.339686213134382 1105 26-27 12.503902267348789 1105 28-29 12.210304140383208 1105 30-31 12.090333709736136 1105 32-33 11.889896232592108 1105 34-35 12.121381436866727 1105 36-37 12.047341952237492 1105 38-39 11.873947011541695 1105 40-41 11.691316092372745 1105 42-43 11.563807128769298 1105 44-45 11.29160182484317 1105 46-47 11.306191391394663 1105 48-49 11.244553831214304 1105 50-51 13.346927416431733 1105 52-53 11.815091364867989 1105 54-55 11.52434930078472 1105 56-57 11.599602378574215 1105 58-59 11.529518846836083 1105 60-61 11.26028342911495 1105 62-63 11.4926993899951 1105 64-65 11.097500976828325 1105 66-67 11.084483510197497 1105 68-69 10.894213961486685 1105 70-71 10.698102236887287 1105 72-73 10.374924281561565 1105 74-75 10.430975531454497 1105 76-77 10.26073459692837 1105 78-79 10.037347897303267 1105 80-81 9.93070379931406 1105 82-83 9.564964732426247 1105 84-85 9.47679192470515 1105 86-87 9.31924818254151 1105 88-89 9.136410934438185 1105 90-91 8.86860981807643 1105 92-93 8.672736607161239 1105 94-95 8.36560193554566 1105 96-97 7.958682496618614 1105 98-99 7.64972628379258 1105 100-101 6.956874369571901 1106 1 8.224602588027956 1106 2 9.200556217261354 1106 3 8.87340639899735 1106 4 10.574280507873404 1106 5 10.606763652596378 1106 6 9.83755301476512 1106 7 9.344707163798287 1106 8 8.833975772442624 1106 9 7.87578640080233 1106 10-11 7.4911509665842 1106 12-13 7.20754626894713 1106 14-15 8.406726620160256 1106 16-17 9.444617032579789 1106 18-19 10.96582466360503 1106 20-21 11.440578834893333 1106 22-23 11.902714256860062 1106 24-25 11.597459493425252 1106 26-27 11.892134754993958 1106 28-29 11.846654025165414 1106 30-31 11.447576610851584 1106 32-33 11.588688411874536 1106 34-35 11.356958073634502 1106 36-37 11.711999410572464 1106 38-39 11.299942705591967 1106 40-41 11.116017092078621 1106 42-43 10.890406733178352 1106 44-45 10.39702519853114 1106 46-47 10.45262050346982 1106 48-49 10.395102962546225 1106 50-51 12.523863104945 1106 52-53 11.345174710816401 1106 54-55 10.574858291136792 1106 56-57 10.265821152824163 1106 58-59 10.343043879487567 1106 60-61 10.390168706830977 1106 62-63 10.400732791108954 1106 64-65 10.297887430361389 1106 66-67 10.107183393411344 1106 68-69 9.799395671019646 1106 70-71 9.359588421434871 1106 72-73 9.414677262245252 1106 74-75 9.157528630515433 1106 76-77 9.1178011141233 1106 78-79 8.831461606743488 1106 80-81 8.376987167355932 1106 82-83 8.269337553482487 1106 84-85 8.219383345445806 1106 86-87 7.977943705253896 1106 88-89 7.625152707923952 1106 90-91 7.680904141014478 1106 92-93 7.6019016429585395 1106 94-95 7.236118976823592 1106 96-97 6.900481030722762 1106 98-99 6.733183495008236 1106 100-101 6.214447854158992 1107 1 7.957503507080332 1107 2 8.794492783655251 1107 3 8.636329970942995 1107 4 10.268807756028558 1107 5 10.611715360371303 1107 6 10.008984220708271 1107 7 9.618527795822263 1107 8 8.304295803050177 1107 9 5.204318036407283 1107 10-11 6.595928855378585 1107 12-13 6.717216411993409 1107 14-15 7.720283399650992 1107 16-17 9.1328060122087 1107 18-19 10.970322212523445 1107 20-21 11.581761684753925 1107 22-23 11.183041058563294 1107 24-25 11.047025840529233 1107 26-27 11.16128649634911 1107 28-29 11.390921439405084 1107 30-31 11.271976777101251 1107 32-33 11.484423860211905 1107 34-35 11.67920029719626 1107 36-37 12.043023414932396 1107 38-39 11.797383589247474 1107 40-41 11.301454281908761 1107 42-43 11.06281850937988 1107 44-45 10.660812360423634 1107 46-47 10.650525730150136 1107 48-49 10.977828686379112 1107 50-51 12.919698330606902 1107 52-53 11.343285134725527 1107 54-55 10.994230643597092 1107 56-57 10.625757185237344 1107 58-59 10.79508814483745 1107 60-61 10.644194205786413 1107 62-63 10.774126376183919 1107 64-65 10.681011024877353 1107 66-67 10.805537273992336 1107 68-69 10.777812115423718 1107 70-71 10.271684598518291 1107 72-73 10.12438304988057 1107 74-75 10.067392548007152 1107 76-77 10.116144810535996 1107 78-79 9.816208062240047 1107 80-81 9.405476001929255 1107 82-83 9.111738237198004 1107 84-85 9.057397267479118 1107 86-87 8.591332721215515 1107 88-89 8.706747163332434 1107 90-91 8.597918308018327 1107 92-93 7.996687486414105 1107 94-95 8.089760780357222 1107 96-97 7.697182323561641 1107 98-99 7.307948364582069 1107 100-101 6.80457017427768 1108 1 6.8503876456026305 1108 2 8.189748754136684 1108 3 8.22119996804561 1108 4 9.166413165450624 1108 5 10.12109620711988 1108 6 9.15901835863647 1108 7 8.55527026057154 1108 8 5.407491058289459 1108 9 4.904964052140894 1108 10-11 4.346162839571857 1108 12-13 3.4433111792476865 1108 14-15 6.321625061359207 1108 16-17 8.114119266773447 1108 18-19 8.747900814303367 1108 20-21 8.860038811610824 1108 22-23 9.935892055418218 1108 24-25 9.836201283888148 1108 26-27 8.209253712970035 1108 28-29 10.151549440183814 1108 30-31 10.571061816645496 1108 32-33 10.915703570948942 1108 34-35 10.576777829595308 1108 36-37 10.305817369000199 1108 38-39 9.951727697735706 1108 40-41 10.330311342244698 1108 42-43 9.547095182892413 1108 44-45 9.476884103998891 1108 46-47 10.09818387741577 1108 48-49 10.744061111287088 1108 50-51 12.641170767543137 1108 52-53 11.347136048406384 1108 54-55 10.71880588459625 1108 56-57 10.576830724824088 1108 58-59 10.555411097864209 1108 60-61 10.675331698005984 1108 62-63 10.643309561129229 1108 64-65 10.467039563636927 1108 66-67 9.743411500176446 1108 68-69 10.295807647788028 1108 70-71 10.25596195753139 1108 72-73 9.975220411672233 1108 74-75 9.797130065270345 1108 76-77 9.301260810941809 1108 78-79 9.163528806126983 1108 80-81 9.144410651789276 1108 82-83 9.052836372545066 1108 84-85 8.914675135590638 1108 86-87 8.629917720086723 1108 88-89 8.30509890007097 1108 90-91 8.12969148776297 1108 92-93 7.862782034308205 1108 94-95 7.793910846485177 1108 96-97 7.552326228410507 1108 98-99 7.422238217985489 1108 100-101 6.776171713820554 1109 1 6.14891183976151 1109 2 7.87095959759483 1109 3 8.031178576861645 1109 4 8.819951912776727 1109 5 9.310767651407836 1109 6 8.23474219795282 1109 7 6.576425729013085 1109 8 6.692041854758621 1109 9 4.569727391486484 1109 10-11 3.3534172115282352 1109 12-13 4.263161395630007 1109 14-15 6.429986661668178 1109 16-17 6.076284998359643 1109 18-19 6.099705103328677 1109 20-21 8.9157768074577 1109 22-23 10.114850909006687 1109 24-25 9.66562744350654 1109 26-27 10.053307947486136 1109 28-29 10.246752334589146 1109 30-31 7.646798235011538 1109 32-33 6.813434083734471 1109 34-35 8.708810481635222 1109 36-37 7.109213961732273 1109 38-39 7.395390553819141 1109 40-41 8.493442804873133 1109 42-43 7.193753303360268 1109 44-45 7.877868284314019 1109 46-47 7.246621876753046 1109 48-49 7.905616906285296 1109 50-51 10.06012463030865 1109 52-53 10.132997105889697 1109 54-55 10.010058934732484 1109 56-57 8.99895065507042 1109 58-59 8.953363047254271 1109 60-61 9.281311098282224 1109 62-63 9.782520657528266 1109 64-65 9.72921121077622 1109 66-67 9.163743505494551 1109 68-69 8.859054429704479 1109 70-71 8.480978718265174 1109 72-73 7.94369753978679 1109 74-75 6.959411138809507 1109 76-77 6.847741646136317 1109 78-79 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48-49 -19.291143455791882 2313 50-51 -16.65354854739502 2313 52-53 -18.82698383741664 2313 54-55 -19.727911527392337 2313 56-57 -19.766413804195896 2313 58-59 -19.544969492050157 2313 60-61 -19.30825536193693 2313 62-63 -18.92472088606392 2313 64-65 -18.632389551491524 2313 66-67 -18.348881554057616 2313 68-69 -17.955857433087328 2313 70-71 -17.68076496920505 2313 72-73 -17.302610225815876 2313 74-75 -16.912194387631654 2313 76-77 -16.55316354681271 2313 78-79 -16.23038175524314 2313 80-81 -15.85749229778256 2313 82-83 -15.428847737826011 2313 84-85 -15.092698794000228 2313 86-87 -14.653383897420412 2313 88-89 -14.281485305447585 2313 90-91 -13.837720500819332 2313 92-93 -13.351775529916344 2313 94-95 -12.829067269594747 2313 96-97 -12.202193276822605 2313 98-99 -11.530188042718605 2313 100-101 -10.380108019766508 2314 1 -18.350373534226105 2314 2 -17.764104718746285 2314 3 -18.493833333571896 2314 4 -20.092863713712077 2314 5 -19.252357646353 2314 6 -19.822427883038127 2314 7 -19.57983915902884 2314 8 -19.349882871301407 2314 9 -18.20778570999041 2314 10-11 -18.229479405908638 2314 12-13 -17.845223549582 2314 14-15 -19.328707954815865 2314 16-17 -20.581170933036258 2314 18-19 -20.731166740406433 2314 20-21 -20.971312282585178 2314 22-23 -20.942319171984256 2314 24-25 -20.985635062448676 2314 26-27 -20.847359036792096 2314 28-29 -20.792075678750535 2314 30-31 -20.919185566798845 2314 32-33 -20.86307854132508 2314 34-35 -20.635401047664445 2314 36-37 -20.13733201254119 2314 38-39 -20.13105060863917 2314 40-41 -20.1406686767848 2314 42-43 -20.064703104452974 2314 44-45 -19.74504738981652 2314 46-47 -19.425602992232186 2314 48-49 -19.291143455791882 2314 50-51 -16.65354854739502 2314 52-53 -18.82698383741664 2314 54-55 -19.727911527392337 2314 56-57 -19.766413804195896 2314 58-59 -19.544969492050157 2314 60-61 -19.30825536193693 2314 62-63 -18.92472088606392 2314 64-65 -18.632389551491524 2314 66-67 -18.348881554057616 2314 68-69 -17.955857433087328 2314 70-71 -17.68076496920505 2314 72-73 -17.302610225815876 2314 74-75 -16.912194387631654 2314 76-77 -16.55316354681271 2314 78-79 -16.23038175524314 2314 80-81 -15.85749229778256 2314 82-83 -15.428847737826011 2314 84-85 -15.092698794000228 2314 86-87 -14.653383897420412 2314 88-89 -14.281485305447585 2314 90-91 -13.837720500819332 2314 92-93 -13.351775529916344 2314 94-95 -12.829067269594747 2314 96-97 -12.202193276822605 2314 98-99 -11.530188042718605 2314 100-101 -10.380108019766508 2315 1 -18.350373534226105 2315 2 -17.764104718746285 2315 3 -18.493833333571896 2315 4 -20.092863713712077 2315 5 -19.252357646353 2315 6 -19.822427883038127 2315 7 -19.57983915902884 2315 8 -19.349882871301407 2315 9 -18.20778570999041 2315 10-11 -18.229479405908638 2315 12-13 -17.845223549582 2315 14-15 -19.328707954815865 2315 16-17 -20.581170933036258 2315 18-19 -20.731166740406433 2315 20-21 -20.971312282585178 2315 22-23 -20.942319171984256 2315 24-25 -20.985635062448676 2315 26-27 -20.847359036792096 2315 28-29 -20.792075678750535 2315 30-31 -20.919185566798845 2315 32-33 -20.86307854132508 2315 34-35 -20.635401047664445 2315 36-37 -20.13733201254119 2315 38-39 -20.13105060863917 2315 40-41 -20.1406686767848 2315 42-43 -20.064703104452974 2315 44-45 -19.74504738981652 2315 46-47 -19.425602992232186 2315 48-49 -19.291143455791882 2315 50-51 -16.65354854739502 2315 52-53 -18.82698383741664 2315 54-55 -19.727911527392337 2315 56-57 -19.766413804195896 2315 58-59 -19.544969492050157 2315 60-61 -19.30825536193693 2315 62-63 -18.92472088606392 2315 64-65 -18.632389551491524 2315 66-67 -18.348881554057616 2315 68-69 -17.955857433087328 2315 70-71 -17.68076496920505 2315 72-73 -17.302610225815876 2315 74-75 -16.912194387631654 2315 76-77 -16.55316354681271 2315 78-79 -16.23038175524314 2315 80-81 -15.85749229778256 2315 82-83 -15.428847737826011 2315 84-85 -15.092698794000228 2315 86-87 -14.653383897420412 2315 88-89 -14.281485305447585 2315 90-91 -13.837720500819332 2315 92-93 -13.351775529916344 2315 94-95 -12.829067269594747 2315 96-97 -12.202193276822605 2315 98-99 -11.530188042718605 2315 100-101 -10.380108019766508 2316 1 -18.350373534226105 2316 2 -17.764104718746285 2316 3 -18.493833333571896 2316 4 -20.092863713712077 2316 5 -19.252357646353 2316 6 -19.822427883038127 2316 7 -19.57983915902884 2316 8 -19.349882871301407 2316 9 -18.20778570999041 2316 10-11 -18.229479405908638 2316 12-13 -17.845223549582 2316 14-15 -19.328707954815865 2316 16-17 -20.581170933036258 2316 18-19 -20.731166740406433 2316 20-21 -20.971312282585178 2316 22-23 -20.942319171984256 2316 24-25 -20.985635062448676 2316 26-27 -20.847359036792096 2316 28-29 -20.792075678750535 2316 30-31 -20.919185566798845 2316 32-33 -20.86307854132508 2316 34-35 -20.635401047664445 2316 36-37 -20.13733201254119 2316 38-39 -20.13105060863917 2316 40-41 -20.1406686767848 2316 42-43 -20.064703104452974 2316 44-45 -19.74504738981652 2316 46-47 -19.425602992232186 2316 48-49 -19.291143455791882 2316 50-51 -16.65354854739502 2316 52-53 -18.82698383741664 2316 54-55 -19.727911527392337 2316 56-57 -19.766413804195896 2316 58-59 -19.544969492050157 2316 60-61 -19.30825536193693 2316 62-63 -18.92472088606392 2316 64-65 -18.632389551491524 2316 66-67 -18.348881554057616 2316 68-69 -17.955857433087328 2316 70-71 -17.68076496920505 2316 72-73 -17.302610225815876 2316 74-75 -16.912194387631654 2316 76-77 -16.55316354681271 2316 78-79 -16.23038175524314 2316 80-81 -15.85749229778256 2316 82-83 -15.428847737826011 2316 84-85 -15.092698794000228 2316 86-87 -14.653383897420412 2316 88-89 -14.281485305447585 2316 90-91 -13.837720500819332 2316 92-93 -13.351775529916344 2316 94-95 -12.829067269594747 2316 96-97 -12.202193276822605 2316 98-99 -11.530188042718605 2316 100-101 -10.380108019766508 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 523898.0 3 9326.0 4 24640.0 5 13889.0 6 12769.0 7 12866.0 8 10893.0 9 11388.0 10 13172.0 11 14239.0 12 16204.0 13 17364.0 14 18506.0 15 19747.0 16 21149.0 17 20702.0 18 20499.0 19 21328.0 20 23015.0 21 25417.0 22 27873.0 23 30810.0 24 33929.0 25 37769.0 26 41537.0 27 46946.0 28 52496.0 29 59734.0 30 67695.0 31 77713.0 32 89563.0 33 102706.0 34 116447.0 35 124738.0 36 109213.0 37 56576.0 38 8467.0 39 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.754562821686825 21.596829707284726 15.125201300956773 22.523406170071677 2 13.84673906622153 20.577822797358575 42.70409023384086 22.871347902579036 3 17.68242792632875 27.080190979883405 31.105234693572843 24.132146400215003 4 11.191131379386933 17.413674740324737 42.044894038646255 29.350299841642073 5 11.510005215400557 38.74937253387941 37.3850147167616 12.355607533958429 6 27.81715281751084 40.460711833129544 18.84275799705807 12.879377352301546 7 24.284208984230624 35.24098935940014 23.76151992441236 16.71328173195688 8 22.80156612862983 39.86072459039467 20.674241406444928 16.66346787453058 9 23.803841842703495 20.407267052273927 21.63203082991874 34.15686027510384 10-11 22.211278907479368 31.120236837079783 28.577717555812676 18.090766699628176 12-13 22.773768989776297 28.338080302102508 29.036421616001405 19.85172909211979 14-15 20.48919260189862 28.944795337104512 27.038603303469927 23.527408757526942 16-17 20.03455665026266 31.345285613636197 28.03899642281562 20.581161313285516 18-19 20.020281659450404 29.826191626063192 30.22079002790474 19.932736686581663 20-21 20.77109336963968 28.791341913005514 30.432631477659676 20.00493323969513 22-23 20.507974415350503 28.55205058688885 30.11031890167154 20.829656096089106 24-25 20.45519697328735 28.714042648814598 30.340386165579442 20.490374212318613 26-27 20.326815599600224 28.823835644494906 30.148767608460698 20.700581147444176 28-29 20.43756747080666 28.75946991779364 30.245850931182865 20.55711168021684 30-31 20.70529389996159 28.573464983887764 30.097509046235693 20.623732069914954 32-33 20.499845354318914 28.79551125307384 30.03923007255171 20.665413320055535 34-35 20.707178858991497 28.65312200714547 30.12480721407551 20.514891919787523 36-37 20.79772320076137 28.49039044801465 29.998334152199448 20.71355219902453 38-39 20.695171452751744 28.648172161823105 30.12909321561224 20.527563169812915 40-41 20.653661214406977 28.63710450785647 30.13906820238083 20.57016607535572 42-43 20.657100628999736 28.568772190321734 29.89587670947664 20.87825047120189 44-45 20.68809979156153 28.900905930298688 29.713792369931596 20.69720190820819 46-47 20.8891088489438 28.786686355278686 29.490246087731958 20.833958708045557 48-49 20.80968282956035 28.883621136569094 29.374348041117177 20.93234799275338 50-51 21.046730827888343 28.971627482032787 28.996557638307006 20.985084051771864 52-53 20.643695410908766 29.15208333879935 29.227415956740643 20.97680529355124 54-55 20.673917859877832 29.126051135815494 29.196409621448062 21.00362138285861 56-57 20.715419994347883 29.21304005656218 29.190212883888627 20.881327065201308 58-59 20.696587459259685 29.067279477711704 29.277081521242497 20.95905154178611 60-61 20.627364815416115 29.1995336403142 29.340148151637557 20.832953392632124 62-63 20.618074799220388 29.05363928577665 29.28608559343321 21.042200321569755 64-65 20.43064311134964 29.007451281667286 29.32230578392174 21.239599823061337 66-67 20.475443504912494 29.002412194174166 29.12493002181229 21.397214279101053 68-69 20.45460271814262 29.09957956303447 29.15551464474504 21.29030307407787 70-71 20.593465940000595 28.786658885668786 29.305183265579306 21.314691908751314 72-73 20.603390095981315 28.942139084556285 29.14072225042933 21.31374856903307 74-75 20.456852258049917 29.14749146815868 28.95857838442984 21.43707788936156 76-77 20.50276072073888 29.08167505757364 29.111320820160163 21.304243401527312 78-79 20.297452648924615 29.224431390513434 28.955791634225186 21.522324326336765 80-81 20.413884147065247 29.225850200052854 28.82971209570534 21.530553557176557 82-83 20.599456593015724 28.968381185029944 29.058860699964367 21.373301521989966 84-85 20.712021114135847 29.065454331870903 28.796037141266538 21.426487412726704 86-87 20.41367032239369 29.24582701703798 28.935001865211095 21.405500795357234 88-89 20.68791306792482 28.913833113265518 28.828530790099542 21.569723028710115 90-91 20.532467971558017 28.977612600194536 29.071012634524656 21.41890679372279 92-93 20.57984742978646 28.963992532400933 28.825315980018352 21.63084405779425 94-95 20.303752394314216 28.926852133455327 28.927462968904617 21.84193250332584 96-97 20.356538774495473 28.808051391102452 28.87794105802301 21.95746877637907 98-99 20.223659980430067 28.76953334615335 28.762263074921872 22.244543598494708 100-101 20.32040412558798 28.78218535835357 28.757759902915243 22.139650613143207 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 113121.0 1 102728.5 2 68633.5 3 35099.0 4 21419.5 5 16499.0 6 15754.5 7 16583.5 8 17122.0 9 16523.5 10 14488.0 11 11648.5 12 8926.0 13 6715.5 14 5298.0 15 4645.0 16 4611.5 17 5137.0 18 6018.0 19 7035.5 20 8095.0 21 9071.5 22 10026.0 23 11117.5 24 12186.5 25 13387.0 26 15073.5 27 17258.0 28 20161.5 29 24745.0 30 30683.0 31 36821.0 32 43557.5 33 50052.5 34 55062.5 35 58416.5 36 61929.5 37 64668.5 38 63865.0 39 61115.0 40 58289.5 41 55638.5 42 53860.5 43 52973.5 44 52833.5 45 53179.5 46 52332.0 47 51063.5 48 49711.5 49 47568.5 50 44564.5 51 41216.0 52 37687.0 53 34051.0 54 30261.0 55 25816.5 56 21307.0 57 16871.0 58 13164.0 59 10359.5 60 7693.5 61 5531.0 62 4019.5 63 2665.0 64 1599.5 65 953.0 66 551.0 67 318.0 68 195.5 69 102.0 70 54.5 71 35.5 72 22.0 73 11.5 74 8.0 75 6.0 76 4.0 77 4.0 78 2.5 79 1.0 80 0.0 81 0.5 82 0.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.45207870541894 2 28.276666733840862 3 22.131416491884583 4 28.344150962115304 5 31.339861631495808 6 24.22838812422265 7 23.107560868862024 8 19.231299867150113 9 11.397342072179189 10-11 14.902155619870538 12-13 15.066990756314244 14-15 19.1174396091082 16-17 22.1847424670601 18-19 26.013542317416665 20-21 28.041247021728783 22-23 27.603038753825697 24-25 26.18690340919367 26-27 27.59244590022979 28-29 24.659594774261123 30-31 26.414649761505775 32-33 25.48800759791801 34-35 23.628135032530395 36-37 26.485647716826673 38-39 26.580027458743423 40-41 23.755481801735883 42-43 24.55325285864948 44-45 23.36088508717402 46-47 26.496938148586214 48-49 22.005929931115446 50-51 27.66315965703957 52-53 21.221619549137145 54-55 22.33541935040488 56-57 24.39448923585221 58-59 23.661954655352467 60-61 23.215892174051437 62-63 23.06250248673697 64-65 21.675949623488645 66-67 22.144102079971397 68-69 23.09420353883986 70-71 21.637944531684774 72-73 21.482952998216785 74-75 18.927181624034304 76-77 21.042264452398555 78-79 20.194603638671047 80-81 21.207409623581654 82-83 21.98833545965492 84-85 19.06315769348622 86-87 20.560858010806776 88-89 19.40406672567661 90-91 20.5273484227241 92-93 20.522000323469577 94-95 19.636566943992744 96-97 18.524368988878535 98-99 17.909957644087353 100-101 19.61036900851407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1935267.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.27347182907883 #Duplication Level Percentage of deduplicated Percentage of total 1 85.44694817003611 60.90100653277827 2 9.251965888056253 13.18839460171951 3 2.4951542751210196 5.335149238111307 4 1.0489294294135805 2.9904336855200224 5 0.5449117042562909 1.9418874501323042 6 0.3086225524618558 1.319796047922509 7 0.19874292033940566 0.991556855382764 8 0.13461014384075531 0.7675305835953858 9 0.09794936218593192 0.6283071995791732 >10 0.4442491092750996 5.339564152382022 >50 0.019055853622879582 0.9356028130538568 >100 0.0085603939989341 0.9929954055743787 >500 7.50493479630494E-5 0.04118751242633054 >1k 1.500986959260988E-4 0.16405467611890362 >5k 0.0 0.0 >10k+ 7.50493479630494E-5 4.462533245703218 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 83427 4.3108780338836965 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.583622828271241E-5 0.0 16-17 0.0 0.0 0.0 5.167245656542482E-5 0.0 18-19 0.0 0.0 0.0 5.167245656542482E-5 0.0 20-21 0.0 0.0 0.0 7.750868484813723E-5 0.0 22-23 0.0 0.0 0.0 1.0334491313084964E-4 0.0 24-25 0.0 0.0 0.0 1.5501736969627446E-4 0.0 26-27 0.0 0.0 0.0 2.325260545444117E-4 0.0 28-29 0.0 0.0 0.0 3.6170719595797376E-4 0.0 30-31 0.0 0.0 0.0 7.750868484813723E-4 0.0 32-33 0.0 5.167245656542482E-5 0.0 0.0029194937959465027 0.0 34-35 0.0 5.167245656542482E-5 0.0 0.005864823820175717 0.0 36-37 0.0 5.167245656542482E-5 0.0 0.011807156325199571 0.0 38-39 0.0 5.167245656542482E-5 0.0 0.022839225801917773 0.0 40-41 0.0 5.167245656542482E-5 0.0 0.033819622822070544 0.0 42-43 0.0 5.167245656542482E-5 0.0 0.043973260537176526 0.0 44-45 0.0 5.167245656542482E-5 0.0 0.05890660048458429 0.0 46-47 0.0 5.167245656542482E-5 0.0 0.07797373695722606 0.0 48-49 0.0 5.167245656542482E-5 0.0 0.09789346896319732 0.0 50-51 0.0 5.167245656542482E-5 0.0 0.12171447143985817 0.0 52-53 0.0 5.167245656542482E-5 0.0 0.1492558907892296 0.0 54-55 0.0 5.167245656542482E-5 0.0 0.17899338954263158 0.0 56-57 0.0 5.167245656542482E-5 0.0 0.21730851608589408 0.0 58-59 0.0 5.167245656542482E-5 0.0 0.2597832753826733 0.0 60-61 0.0 5.167245656542482E-5 0.0 0.3054100545299434 0.0 62-63 0.0 5.167245656542482E-5 0.0 0.35586820836608074 0.0 64-65 0.0 5.167245656542482E-5 0.0 0.4091166748567511 0.0 66-67 0.0 5.167245656542482E-5 0.0 0.47231208923626555 0.0 68-69 0.0 5.167245656542482E-5 0.0 0.5441368038622061 0.0 70-71 0.0 5.167245656542482E-5 0.0 0.6236090420598295 0.0 72-73 0.0 5.167245656542482E-5 0.0 0.7098503720675235 5.167245656542482E-5 74-75 0.0 5.167245656542482E-5 0.0 0.7990111958711641 5.167245656542482E-5 76-77 0.0 5.167245656542482E-5 0.0 0.8981189675636488 5.167245656542482E-5 78-79 0.0 5.167245656542482E-5 0.0 1.0165264017833198 5.167245656542482E-5 80-81 0.0 5.167245656542482E-5 0.0 1.1502805556029219 5.167245656542482E-5 82-83 0.0 5.167245656542482E-5 0.0 1.2905712751780505 5.167245656542482E-5 84-85 0.0 5.167245656542482E-5 0.0 1.431662917829943 5.167245656542482E-5 86-87 0.0 5.167245656542482E-5 0.0 1.5852851311989506 5.167245656542482E-5 88-89 0.0 5.167245656542482E-5 0.0 1.7553908582123294 5.167245656542482E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1605 0.0 58.56319 1 GTATCAA 3180 0.0 43.372913 1 ATCAACG 3430 0.0 37.637775 3 TCAACGC 3510 0.0 37.35096 4 CAACGCA 3565 0.0 36.484573 5 TATCAAC 3905 0.0 34.27114 2 AACGCAG 3775 0.0 34.110497 6 ACGCAGA 4585 0.0 28.50948 7 CGCAGAG 4485 0.0 27.51051 8 GTACATG 3405 0.0 25.50432 1 GTGGTAT 925 0.0 25.403763 1 GCAGAGT 4900 0.0 23.503883 9 TACATGG 3625 0.0 23.250067 2 GAGTACA 2685 0.0 22.640429 1 ACATGGG 3530 0.0 22.341375 3 GAGTACT 3150 0.0 21.788797 12-13 TATACCG 140 3.3323624E-4 21.52248 5 TGGTATC 1005 0.0 20.838482 2 AGAGTAC 4505 0.0 20.225355 10-11 AGTACAT 2710 0.0 20.167997 2 >>END_MODULE