FastQCFastQC Report
Sat 18 Jun 2016
SRR1930988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935267
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44000.22735880888786922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42000.21702431757478427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.11553961288028991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.055.8495141
GTATCAA50600.039.351681
ATCAACG61300.032.0926743
CAACGCA62150.031.52595
TCAACGC62800.031.4009864
AACGCAG65950.029.779796
TATCAAC66500.029.654632
ACGCAGA75600.025.6647857
CGCAGAG76950.025.1528788
GTGGTAT13750.024.8845371
GTACATG56650.024.0758571
ACATGGG55900.023.1218813
TACATGG58200.022.616372
GAGTACT54550.021.0888612-13
GCAGAGT87100.021.0779789
GGCGTCG1656.388348E-520.1256648
GTACTTT59500.019.57359714-15
TGGTATC15200.019.3826962
AGAGTAC80850.019.21619610-11
CATGGGG32150.019.2139744
Produced by FastQC (version 0.11.5)