##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930988_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1935267 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.379572431090903 31.0 31.0 34.0 30.0 34.0 2 31.55543085269371 31.0 31.0 34.0 30.0 34.0 3 31.619311443847284 31.0 31.0 34.0 30.0 34.0 4 35.38742871138711 37.0 35.0 37.0 33.0 37.0 5 35.27149483766323 37.0 35.0 37.0 33.0 37.0 6 35.29810150227333 37.0 35.0 37.0 32.0 37.0 7 35.182176412867065 37.0 35.0 37.0 32.0 37.0 8 35.18422419232075 37.0 35.0 37.0 32.0 37.0 9 36.68951002626511 39.0 35.0 39.0 32.0 39.0 10-11 36.63197274587951 39.0 35.0 39.0 32.0 39.0 12-13 36.52477332585116 38.0 35.0 39.0 32.0 39.0 14-15 37.60356116236157 39.0 36.0 41.0 32.0 41.0 16-17 37.56262856753099 39.0 36.0 41.0 32.0 41.0 18-19 37.58803591442421 39.0 36.0 41.0 32.0 41.0 20-21 37.57655274440168 39.0 36.0 41.0 32.0 41.0 22-23 37.48341753360131 39.0 36.0 41.0 32.0 41.0 24-25 37.37011585481486 39.0 36.0 41.0 32.0 41.0 26-27 37.13773293297514 39.0 36.0 40.0 31.0 41.0 28-29 36.95980502948689 39.0 36.0 40.0 31.0 41.0 30-31 36.74386144134117 38.5 35.5 40.0 30.0 41.0 32-33 36.58360474291145 38.0 35.0 40.0 30.0 41.0 34-35 36.66689221693957 38.5 35.0 40.0 30.0 41.0 36-37 36.739686048488394 39.0 35.0 40.0 30.0 41.0 38-39 36.73822526814129 39.0 35.0 40.0 30.0 41.0 40-41 36.63735546567993 39.0 35.0 40.0 30.0 41.0 42-43 36.51220322570477 39.0 35.0 40.0 30.0 41.0 44-45 36.35537473640588 39.0 35.0 40.0 30.0 41.0 46-47 36.13525988920392 38.5 35.0 40.0 29.5 41.0 48-49 35.93118701450498 38.5 35.0 40.0 29.0 41.0 50-51 35.68346564065837 38.0 35.0 40.0 28.0 41.0 52-53 35.48537617806742 38.0 34.0 40.0 27.5 41.0 54-55 35.34867152697793 38.0 34.0 40.0 27.0 41.0 56-57 35.19692347360855 38.0 34.0 40.0 27.0 41.0 58-59 35.0063805149367 38.0 34.0 40.0 26.5 41.0 60-61 34.71453680551572 38.0 33.5 40.0 26.0 41.0 62-63 34.47460505449635 37.5 33.0 40.0 26.0 41.0 64-65 34.17116475401069 37.0 33.0 40.0 26.0 41.0 66-67 33.86668738732175 36.5 32.5 39.0 25.0 41.0 68-69 33.53781287026545 36.0 32.0 39.0 25.0 40.5 70-71 33.16950606815494 36.0 32.0 39.0 24.0 40.0 72-73 32.76427516203191 35.0 31.0 38.5 22.5 40.0 74-75 32.25340637751793 35.0 31.0 37.5 22.0 40.0 76-77 30.855105264544893 33.5 29.5 36.0 20.5 39.0 78-79 31.602174790351924 34.0 31.0 36.0 22.0 39.0 80-81 31.56874426112779 34.5 31.0 36.0 22.0 39.0 82-83 31.288308538305053 34.0 31.0 36.0 21.5 37.5 84-85 30.9384051399626 34.0 31.0 35.5 20.5 37.0 86-87 30.58914428861754 34.0 30.0 35.0 20.0 37.0 88-89 30.23846451161519 34.0 30.0 35.0 18.5 36.0 90-91 29.9027459776868 34.0 30.0 35.0 17.5 36.0 92-93 29.586928832042297 34.0 29.5 35.0 10.5 36.0 94-95 29.211748818121734 34.0 29.0 35.0 4.5 35.0 96-97 28.797898946243592 34.0 29.0 35.0 2.0 35.0 98-99 28.32530653393046 34.0 29.0 35.0 2.0 35.0 100-101 27.216896169882503 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2949359302133736 1101 2 0.3557823348674667 1101 3 0.2913332574821581 1101 4 0.11073227532864394 1101 5 0.17801226692709093 1101 6 0.28688545412014577 1101 7 0.3381042479365135 1101 8 0.2727001754049141 1101 9 0.5353677399556034 1101 10-11 0.3210353712864915 1101 12-13 0.4251500479752153 1101 14-15 0.5025872972531076 1101 16-17 0.5026879607919952 1101 18-19 0.45036322484991587 1101 20-21 0.33818630577773945 1101 22-23 0.5359990627070133 1101 24-25 0.3519002782106213 1101 26-27 0.4805500697910077 1101 28-29 0.5191178007802222 1101 30-31 0.3834701946511103 1101 32-33 0.3518345835253385 1101 34-35 0.3729263256399875 1101 36-37 0.49999896289923385 1101 38-39 0.3859738644166697 1101 40-41 0.400605612080831 1101 42-43 0.2531111029085409 1101 44-45 0.35311360580399054 1101 46-47 0.24977173571940625 1101 48-49 0.32481593958301147 1101 50-51 0.21715336920782136 1101 52-53 0.12520368950507788 1101 54-55 0.05204408760324952 1101 56-57 0.24215408638337976 1101 58-59 0.13938185000587566 1101 60-61 0.09472071635787671 1101 62-63 -0.13442352655509637 1101 64-65 -0.24268626397162762 1101 66-67 -0.006293870333507812 1101 68-69 -0.012367509205326144 1101 70-71 -0.11615887366039601 1101 72-73 -0.006451620489151821 1101 74-75 0.12475717779954465 1101 76-77 0.04887930312953159 1101 78-79 0.18249724711479232 1101 80-81 0.23651168262188094 1101 82-83 0.18047080280166483 1101 84-85 0.04445150005037135 1101 86-87 0.10340755552538639 1101 88-89 0.033190043370680655 1101 90-91 0.13182866797139425 1101 92-93 0.09678263494524941 1101 94-95 0.1895300870077179 1101 96-97 0.31737251188936355 1101 98-99 0.34050701486774315 1101 100-101 0.5702287013690004 1102 1 0.2587941603644346 1102 2 0.37933854520851895 1102 3 0.43010688599630953 1102 4 0.1443966818317861 1102 5 0.23161942364467336 1102 6 0.3492353155687411 1102 7 0.375634498120057 1102 8 0.3702193513639571 1102 9 0.4725155833245367 1102 10-11 0.3792092860495657 1102 12-13 0.41477982775140276 1102 14-15 0.5425982478813509 1102 16-17 0.48890191085416745 1102 18-19 0.5221062781223722 1102 20-21 0.5084156757087541 1102 22-23 0.481638710408248 1102 24-25 0.5225544484718796 1102 26-27 0.5006290230790427 1102 28-29 0.6185567973763781 1102 30-31 0.4793646978312367 1102 32-33 0.6979776387753418 1102 34-35 0.6776624105555626 1102 36-37 0.6838650784913938 1102 38-39 0.5462453312323134 1102 40-41 0.6230097817241074 1102 42-43 0.5708135473952822 1102 44-45 0.761478847570622 1102 46-47 0.7471565625486889 1102 48-49 0.6433467261742933 1102 50-51 0.4641582611476096 1102 52-53 0.4805169689339692 1102 54-55 0.5524771688826178 1102 56-57 0.5082155433854822 1102 58-59 0.5111184762730474 1102 60-61 0.4019021531890701 1102 62-63 0.2604735548429318 1102 64-65 0.21878322134884343 1102 66-67 0.1475330405264046 1102 68-69 0.3947415002201211 1102 70-71 0.3389405842464299 1102 72-73 0.2958671688608021 1102 74-75 0.5149552531586608 1102 76-77 0.46135374960370257 1102 78-79 0.6547236853426206 1102 80-81 0.5149557540802903 1102 82-83 0.3326677262436739 1102 84-85 0.48611159551612104 1102 86-87 0.31988948698879227 1102 88-89 0.4068070630694507 1102 90-91 0.3993364483556796 1102 92-93 0.4003859601789088 1102 94-95 0.5749809537882555 1102 96-97 0.79339533270198 1102 98-99 0.8864989118972311 1102 100-101 1.1150557036901354 1103 1 0.3535010857503167 1103 2 0.41436473465068246 1103 3 0.3141153702718462 1103 4 0.17589335506480808 1103 5 0.3008713629590716 1103 6 0.3441548277228961 1103 7 0.4808841436088258 1103 8 0.321928803317185 1103 9 0.41339918607040715 1103 10-11 0.4758492873932241 1103 12-13 0.37988944430342286 1103 14-15 0.48453626660146654 1103 16-17 0.4646083539608057 1103 18-19 0.5411252357753753 1103 20-21 0.5642060258050847 1103 22-23 0.38183858036081375 1103 24-25 0.41321034823457126 1103 26-27 0.47165819737379877 1103 28-29 0.48929264512010207 1103 30-31 0.47672091983324805 1103 32-33 0.571928441494336 1103 34-35 0.5677561310879398 1103 36-37 0.3822626397420592 1103 38-39 0.5309246875215052 1103 40-41 0.5848246632777645 1103 42-43 0.5921446860781572 1103 44-45 0.6105837517026274 1103 46-47 0.6528276625052669 1103 48-49 0.5459253604842687 1103 50-51 0.5855508244497614 1103 52-53 0.6130360261620424 1103 54-55 0.5590649592998957 1103 56-57 0.6325816821043446 1103 58-59 0.7913720207855022 1103 60-61 0.5278216629438219 1103 62-63 0.31287559337248183 1103 64-65 0.36285406600934067 1103 66-67 0.44153982122851687 1103 68-69 0.4036936585996713 1103 70-71 0.36913486359888026 1103 72-73 0.44299748742903233 1103 74-75 0.5654954778863868 1103 76-77 0.5540772116488029 1103 78-79 0.6295549475945528 1103 80-81 0.561751716371333 1103 82-83 0.5788067330363695 1103 84-85 0.5353791789132352 1103 86-87 0.41473209839889336 1103 88-89 0.48274384849838015 1103 90-91 0.40971169039507416 1103 92-93 0.27390691053294347 1103 94-95 0.5208160766734871 1103 96-97 0.5274252916002737 1103 98-99 0.5873713334428778 1103 100-101 0.8805894140490658 1104 1 0.29809075899149917 1104 2 0.42516568015743417 1104 3 0.432422201307304 1104 4 0.27306555410854827 1104 5 0.3350168654118235 1104 6 0.33670782734187554 1104 7 0.2291567065938338 1104 8 0.30728491512969214 1104 9 0.46413835592121444 1104 10-11 0.4083571138624009 1104 12-13 0.4997014896007883 1104 14-15 0.47105586771132124 1104 16-17 0.47388879656690364 1104 18-19 0.47482760658936485 1104 20-21 0.47078718757440896 1104 22-23 0.4374401345464918 1104 24-25 0.43591349900802356 1104 26-27 0.42810420996542575 1104 28-29 0.63201853774369 1104 30-31 0.5226923038014419 1104 32-33 0.4675054860810235 1104 34-35 0.36603388982127427 1104 36-37 0.47156883414904627 1104 38-39 0.5106548191590718 1104 40-41 0.42782539307138023 1104 42-43 0.46749856431609516 1104 44-45 0.5796291553764448 1104 46-47 0.45058154647643534 1104 48-49 0.4942058066736337 1104 50-51 0.5992500419820104 1104 52-53 0.5680327235771827 1104 54-55 0.6832782214773587 1104 56-57 0.7021550215849857 1104 58-59 0.5314664759067753 1104 60-61 0.463389247685285 1104 62-63 0.4240201749236334 1104 64-65 0.39560113578644973 1104 66-67 0.4693764907896707 1104 68-69 0.43286450077672356 1104 70-71 0.529130109787765 1104 72-73 0.5297937173462728 1104 74-75 0.677087491005949 1104 76-77 0.6670607297795783 1104 78-79 0.621558033988503 1104 80-81 0.6080731862784035 1104 82-83 0.5516075280455865 1104 84-85 0.6353383295815789 1104 86-87 0.736707008309395 1104 88-89 0.7715301097453846 1104 90-91 0.6471176145037028 1104 92-93 0.6067613591087664 1104 94-95 0.7819804259567746 1104 96-97 0.7213261997215277 1104 98-99 0.7523693088926642 1104 100-101 1.0954903424755393 1105 1 0.28025106742163786 1105 2 0.3368145169757817 1105 3 0.3254786866109036 1105 4 0.1966081386874592 1105 5 0.2123818906039574 1105 6 0.2357149068096973 1105 7 0.3452297980823573 1105 8 0.38221957183002786 1105 9 0.49344024231443484 1105 10-11 0.4173621774013654 1105 12-13 0.40304374619017125 1105 14-15 0.5599086879306938 1105 16-17 0.5309662066075305 1105 18-19 0.474530797686171 1105 20-21 0.4374120553008751 1105 22-23 0.3072829781269206 1105 24-25 0.43236969603611897 1105 26-27 0.4655466589167361 1105 28-29 0.3614855349885886 1105 30-31 0.4543010452596121 1105 32-33 0.5530773646407283 1105 34-35 0.5014010713829862 1105 36-37 0.5308460945877513 1105 38-39 0.485333171547623 1105 40-41 0.28945570985123226 1105 42-43 0.3131265447576297 1105 44-45 0.5083973437864984 1105 46-47 0.4213005866803812 1105 48-49 0.5198149624784278 1105 50-51 0.3738346664670118 1105 52-53 0.43173698239547775 1105 54-55 0.4933378460030511 1105 56-57 0.46845294610832866 1105 58-59 0.5880182795818101 1105 60-61 0.5875511665333377 1105 62-63 0.5150949347269886 1105 64-65 0.4970430480868018 1105 66-67 0.5426757339211576 1105 68-69 0.49838951305947177 1105 70-71 0.3383340690069474 1105 72-73 0.4112567515587031 1105 74-75 0.4855372789148831 1105 76-77 0.536353901407665 1105 78-79 0.6617533767975452 1105 80-81 0.5350793552067259 1105 82-83 0.6388021479569623 1105 84-85 0.5992814538806819 1105 86-87 0.5512451571472639 1105 88-89 0.6253299263818874 1105 90-91 0.6244465529416097 1105 92-93 0.5234954752983505 1105 94-95 0.5662210474116023 1105 96-97 0.5208210316658359 1105 98-99 0.470057654661435 1105 100-101 0.44425510357401876 1106 1 0.2585101581261604 1106 2 0.23465299567356013 1106 3 0.1747959035453981 1106 4 0.08006314510410562 1106 5 0.04153428858426622 1106 6 0.18012510489156597 1106 7 0.22514939792725386 1106 8 0.228369606356722 1106 9 0.31819645291501786 1106 10-11 0.1862916270153434 1106 12-13 0.23316250530201188 1106 14-15 0.3549697790470816 1106 16-17 0.34160363445719355 1106 18-19 0.34572143915539044 1106 20-21 0.20694964303255148 1106 22-23 0.1964285253193978 1106 24-25 0.15131782961694284 1106 26-27 0.22438131126538963 1106 28-29 0.3046783203407273 1106 30-31 0.23998924031113233 1106 32-33 0.22488075839464727 1106 34-35 0.38703837421827103 1106 36-37 0.42874764872102133 1106 38-39 0.40410314827676075 1106 40-41 0.29244218966005064 1106 42-43 0.15795654111629887 1106 44-45 0.35883139370223915 1106 46-47 0.3623806290670828 1106 48-49 0.36693147178608854 1106 50-51 0.2490113345111098 1106 52-53 0.21142039598803564 1106 54-55 0.31048875017521027 1106 56-57 0.2506367608833884 1106 58-59 0.2061748221296611 1106 60-61 0.35631627906673913 1106 62-63 0.2219336075379914 1106 64-65 0.115630890234101 1106 66-67 -0.015814174236467693 1106 68-69 0.01864661535350365 1106 70-71 0.09752626247282592 1106 72-73 0.027267365506581598 1106 74-75 0.19658757242591918 1106 76-77 0.12561155240116761 1106 78-79 0.09371100647041786 1106 80-81 0.13820405545634173 1106 82-83 0.22609377740344172 1106 84-85 0.27790608662254 1106 86-87 0.09908008998272066 1106 88-89 0.10395405906623623 1106 90-91 0.15565877871120648 1106 92-93 0.20735957421518236 1106 94-95 0.3561951898925315 1106 96-97 0.2889684595291335 1106 98-99 0.4549195501430532 1106 100-101 0.7290153510024702 1107 1 0.1275830409889558 1107 2 0.16269634169581693 1107 3 0.1691684624415437 1107 4 0.16333936452063824 1107 5 0.1781879360303833 1107 6 0.22791362650815472 1107 7 0.29030192649108955 1107 8 0.2296825383237291 1107 9 0.1965245427779223 1107 10-11 0.229021782337675 1107 12-13 0.22965997715103725 1107 14-15 0.23225674413740904 1107 16-17 0.3312969808923185 1107 18-19 0.24650080327794655 1107 20-21 0.3887859036261574 1107 22-23 0.26745484176077383 1107 24-25 0.25627325323705463 1107 26-27 0.31706274772857057 1107 28-29 0.266650085422242 1107 30-31 0.35624220954668573 1107 32-33 0.3518539410022896 1107 34-35 0.2150325885096862 1107 36-37 0.27490477667546287 1107 38-39 0.23356168082068507 1107 40-41 0.1256585426818546 1107 42-43 0.22649324128062887 1107 44-45 0.15141704393385425 1107 46-47 0.3340431807792541 1107 48-49 0.26658163184267636 1107 50-51 0.30650784574535095 1107 52-53 0.4373623627436132 1107 54-55 0.3668911514369526 1107 56-57 0.36827216215338154 1107 58-59 0.3347231102260153 1107 60-61 0.4481994969336185 1107 62-63 0.31647296659599533 1107 64-65 0.2826980424378718 1107 66-67 0.28203490189432046 1107 68-69 0.2924713760943618 1107 70-71 0.25995617750383815 1107 72-73 0.36299260215086804 1107 74-75 0.6603243639724994 1107 76-77 0.5142738206081248 1107 78-79 0.5950500869776469 1107 80-81 0.4167809693230531 1107 82-83 0.4510352184357309 1107 84-85 0.5107168620230915 1107 86-87 0.4445250142758148 1107 88-89 0.45077522231845535 1107 90-91 0.39035224900784016 1107 92-93 0.4019239808222288 1107 94-95 0.4610962520737658 1107 96-97 0.6463880390055543 1107 98-99 0.8110220689355963 1107 100-101 1.1078664116698391 1108 1 0.07062251393956132 1108 2 0.12612580048855193 1108 3 0.06623781042082655 1108 4 0.04790929517540121 1108 5 0.13318893686120958 1108 6 0.19312995677684341 1108 7 0.09998366228820288 1108 8 0.2140234203892959 1108 9 0.32638577004329505 1108 10-11 0.13405116289194297 1108 12-13 0.18544359112831188 1108 14-15 0.22588273137247228 1108 16-17 0.2909244250445866 1108 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-2.943875128235085 2316 76-77 -2.043127675556221 2316 78-79 -1.757481595622167 2316 80-81 -1.2456459300681928 2316 82-83 -1.0212651274400208 2316 84-85 -1.0565717550997995 2316 86-87 -0.824400865992942 2316 88-89 -1.155211816185055 2316 90-91 -1.1499425481609329 2316 92-93 -1.1614629891048125 2316 94-95 -1.3392996273513944 2316 96-97 -1.3836213086199827 2316 98-99 -1.5527815302403987 2316 100-101 -1.9345992932935197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 16.0 5 19.0 6 71.0 7 192.0 8 505.0 9 1015.0 10 1854.0 11 2870.0 12 4062.0 13 5362.0 14 6966.0 15 8953.0 16 10955.0 17 12926.0 18 11057.0 19 8702.0 20 8641.0 21 9033.0 22 9876.0 23 11765.0 24 14503.0 25 18549.0 26 24300.0 27 31532.0 28 40263.0 29 51173.0 30 63565.0 31 77442.0 32 94024.0 33 114734.0 34 143886.0 35 179290.0 36 235814.0 37 326560.0 38 333527.0 39 71243.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.22766674848528 23.137597374914414 15.644476597723719 21.990259278876586 2 14.6651522026047 22.216267948517867 41.75740187420096 21.36117797467648 3 18.07789791884665 27.315996847911737 31.74034027051118 22.86576496273043 4 12.109760181705152 18.57777828389463 40.624849566830115 28.687611967570103 5 11.6164306830237 39.2651944236172 36.91581323799253 12.202561655366564 6 28.296117011546755 40.91730215340352 18.179243947331162 12.607336887718562 7 24.902261497902614 33.98695579832437 23.876459360108637 17.234323343664386 8 23.53652134192343 38.86625617749006 20.65870491284856 16.93851756773795 9 24.37932336985026 17.300403510213318 22.190374764825734 36.12989835511069 10-11 23.285236610762237 28.285967776022638 29.626738842753998 18.80205677046113 12-13 23.797233146640746 25.576625860927717 30.077761879885305 20.54837911254623 14-15 20.893680303544677 27.56170078857336 27.53258335929874 24.012035548583217 16-17 20.106011211889626 30.824661403310238 28.414451339272567 20.65487604552757 18-19 20.056457326043382 29.46898283285975 30.779783874783167 19.694775966313692 20-21 20.66670903808105 28.58652061963543 30.92562938343908 19.82114095884444 22-23 20.51302190379218 28.353576537708015 30.74069949828408 20.39270206021573 24-25 20.390160570612288 28.63385668396586 30.69898558561678 20.27699715980508 26-27 20.327242070407582 28.79717817790222 30.570970959901633 20.304608791788564 28-29 20.32801226499964 28.79215899854438 30.62224617532604 20.257582561129933 30-31 20.63033784450688 28.64203323719809 30.419234766198084 20.308394152096948 32-33 20.53624382782331 28.785969961104335 30.330451627475107 20.347334583597252 34-35 20.665882157163402 28.65340855942246 30.4891763011979 20.191532982216234 36-37 20.716446754803655 28.510524507232933 30.47937082901648 20.29365790894693 38-39 20.576635548547596 28.66068934905663 30.578699870120847 20.183975232274932 40-41 20.519936255243596 28.696663631694207 30.52291783147945 20.260482281582746 42-43 20.623033607990838 28.566792212570853 30.32677765971961 20.4833965197187 44-45 20.570425006239983 28.888828024997142 30.131812241324873 20.408934727437998 46-47 20.798149906655556 28.8591823155882 29.82867257094501 20.513995206811238 48-49 20.723698065175615 28.817253034637385 29.78808894006228 20.670959960124723 50-51 20.697689108043544 29.03352486354896 29.5060425729318 20.762743455475697 52-53 20.834856203545993 29.03422222394663 29.50405516734759 20.62686640515978 54-55 20.81597878123367 29.11533517908761 29.463649373530604 20.60503666614812 56-57 20.842418694255674 29.184440126018124 29.348667794762957 20.624473384963245 58-59 20.67778065764513 29.089686563334272 29.61584091279678 20.616691866223817 60-61 20.713755596950737 29.247428875493085 29.58638246267643 20.452433064879745 62-63 20.725277628144035 29.156989392118675 29.575736543322424 20.541996436414866 64-65 20.54134679362047 28.951833406413876 29.546207404901843 20.960612395063812 66-67 20.553800230739302 29.02308273718773 29.512820951170198 20.910296080902775 68-69 20.61050037647316 29.096467005954413 29.474934554278832 20.818098063293593 70-71 20.67789578256824 28.84756577459948 29.605905321248038 20.86863312158424 72-73 20.674366366304195 28.970736655780467 29.488279652814796 20.86661732510054 74-75 20.562067127706186 29.249555452088966 29.33674203058966 20.851635389615183 76-77 20.474761465663928 29.2038998263251 29.42778430752602 20.893554400484955 78-79 20.398420786093 29.324389966617403 29.342941595956884 20.934247651332722 80-81 20.560367846358986 29.28692692910969 29.27881434294899 20.873890881582334 82-83 20.70024446239201 29.076582197701917 29.385583487963157 20.837589851942912 84-85 20.771604124908862 29.174372321751985 29.159904033913662 20.894119519425487 86-87 20.463558775094082 29.372768718734932 29.24358757732137 20.92008492884961 88-89 20.63172308666137 29.135479037081534 29.152220951938045 21.080576924319054 90-91 20.595909209059666 29.200073173491976 29.307275439803178 20.896742177645187 92-93 20.651541639048567 29.174092866584232 29.222416464133943 20.95194903023326 94-95 20.333122416778174 29.11506251664876 29.266054311056017 21.285760755517053 96-97 20.403412556784872 29.005673384697545 29.23556444093301 21.355349617584572 98-99 20.283273574137315 29.162203458230827 29.180159636887314 21.374363330744544 100-101 20.254104472406134 29.12409502151383 29.1884013937095 21.43339911237054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 133.0 1 93.5 2 87.0 3 124.0 4 122.0 5 117.5 6 199.0 7 478.0 8 890.5 9 1194.0 10 1126.0 11 956.0 12 975.0 13 1090.5 14 1251.0 15 1462.5 16 1713.5 17 1933.5 18 2124.5 19 2315.0 20 2643.0 21 3098.5 22 3636.0 23 4389.5 24 5497.0 25 7098.0 26 9383.0 27 12499.0 28 16721.5 29 23938.5 30 33296.5 31 42835.0 32 53974.0 33 64688.5 34 72531.5 35 77057.0 36 83818.0 37 90331.0 38 88966.5 39 84410.0 40 79632.0 41 75133.0 42 72698.5 43 72214.5 44 72089.0 45 73274.0 46 73015.0 47 70831.0 48 69439.5 49 67014.0 50 63123.5 51 58204.0 52 53065.0 53 47800.5 54 42649.5 55 36474.5 56 29129.5 57 22376.0 58 17192.5 59 13287.5 60 9609.0 61 6741.0 62 4733.5 63 2932.0 64 1602.5 65 921.0 66 453.0 67 226.5 68 127.0 69 61.5 70 32.5 71 20.5 72 10.0 73 6.0 74 4.5 75 2.5 76 3.0 77 2.5 78 1.0 79 0.5 80 0.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0047538660040190835 2 0.007699196028248299 3 0.0021702431757478426 4 0.17289603966791145 5 0.0017568635232244438 6 2.583622828271241E-4 7 5.1672456565424826E-5 8 7.750868484813723E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.3921588080611095E-4 24-25 6.459057070678102E-4 26-27 0.0032812009919044763 28-29 2.066898262616993E-4 30-31 0.007544178658552024 32-33 0.024570253096859503 34-35 0.015501736969627446 36-37 0.001136794044439346 38-39 3.3587096767526137E-4 40-41 0.002531950371705816 42-43 0.01296978659792163 44-45 0.008577627789860522 46-47 0.011858828781764997 48-49 0.012065518608026696 50-51 0.19661369723144145 52-53 0.11572046647826889 54-55 0.019428843668599732 56-57 0.007673359799965585 58-59 0.019558024810013296 60-61 0.12434976672469483 62-63 0.23908845652822067 64-65 0.24536666000091978 66-67 0.25577865999885285 68-69 0.2506372505705931 70-71 0.2377708088858023 72-73 0.16312994537704617 74-75 0.284069329968423 76-77 0.22531774685353492 78-79 0.0065624019838089525 80-81 1.2918114141356206E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.325260545444117E-4 90-91 0.007234143919159476 92-93 0.020384784115060093 94-95 0.0032295285353390515 96-97 1.2918114141356206E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1935267.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.0467156485896 #Duplication Level Percentage of deduplicated Percentage of total 1 73.36085954525467 39.64913515578499 2 15.159146101302442 16.386041176250373 3 4.980012335683762 8.074599318995064 4 2.139579732184031 4.625490295713432 5 1.1751180781703003 3.1755636312193656 6 0.7071288874089056 2.2930796342815585 7 0.4759754263775502 1.8007435967600554 8 0.32908658605670094 1.42288393122973 9 0.24634112081191062 1.1982535658168572 >10 1.292176760643492 12.48836115163699 >50 0.08607673038969656 3.1961645456142094 >100 0.04639081505149196 4.407883342851014 >500 0.0015330041200279432 0.542899771943804 >1k 5.748765450104785E-4 0.7389008819025312 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4400 0.22735880888786922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4200 0.21702431757478427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2236 0.11553961288028991 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.167245656542482E-5 7 0.0 0.0 0.0 0.0 5.167245656542482E-5 8 0.0 0.0 0.0 0.0 5.167245656542482E-5 9 0.0 0.0 0.0 0.0 5.167245656542482E-5 10-11 0.0 0.0 0.0 0.0 5.167245656542482E-5 12-13 0.0 0.0 0.0 0.0 5.167245656542482E-5 14-15 0.0 0.0 0.0 0.0 7.750868484813723E-5 16-17 0.0 0.0 0.0 0.0 1.0334491313084964E-4 18-19 0.0 0.0 0.0 0.0 1.0334491313084964E-4 20-21 0.0 0.0 0.0 0.0 1.0334491313084964E-4 22-23 0.0 0.0 0.0 5.167245656542482E-5 1.0334491313084964E-4 24-25 0.0 0.0 0.0 1.2918114141356204E-4 1.0334491313084964E-4 26-27 0.0 0.0 0.0 2.5836228282712407E-4 1.0334491313084964E-4 28-29 0.0 0.0 0.0 4.1337965252339856E-4 1.0334491313084964E-4 30-31 0.0 0.0 0.0 0.0012401389575701957 1.0334491313084964E-4 32-33 0.0 0.0 0.0 0.0048313746888672205 1.0334491313084964E-4 34-35 0.0 0.0 0.0 0.010153637715105978 1.0334491313084964E-4 36-37 0.0 0.0 0.0 0.021030689822127902 1.0334491313084964E-4 38-39 0.0 0.0 0.0 0.03942608435941914 1.0334491313084964E-4 40-41 0.0 0.0 0.0 0.06025008435528534 1.0334491313084964E-4 42-43 0.0 0.0 0.0 0.08228838708043903 1.0334491313084964E-4 44-45 0.0 0.0 0.0 0.10957144414698333 1.2918114141356204E-4 46-47 0.0 0.0 0.0 0.14643974190641396 1.5501736969627446E-4 48-49 0.0 0.0 0.0 0.18503906696078629 1.5501736969627446E-4 50-51 0.0 0.0 0.0 0.22753966248584823 1.5501736969627446E-4 52-53 0.0 0.0 0.0 0.2729597518068566 1.5501736969627446E-4 54-55 0.0 0.0 0.0 0.3210409726409844 1.5501736969627446E-4 56-57 0.0 0.0 0.0 0.3812652207679871 1.5501736969627446E-4 58-59 0.0 0.0 0.0 0.45037713142424274 1.5501736969627446E-4 60-61 0.0 0.0 0.0 0.5252246847592605 1.5501736969627446E-4 62-63 0.0 0.0 0.0 0.6062729328821295 1.5501736969627446E-4 64-65 0.0 0.0 0.0 0.6915841586716458 1.5501736969627446E-4 66-67 5.167245656542482E-5 0.0 0.0 0.7934822430186635 1.5501736969627446E-4 68-69 5.167245656542482E-5 0.0 0.0 0.9103395035413718 1.5501736969627446E-4 70-71 5.167245656542482E-5 0.0 0.0 1.0390039203892796 1.5501736969627446E-4 72-73 5.167245656542482E-5 0.0 0.0 1.1788037516270364 1.5501736969627446E-4 74-75 5.167245656542482E-5 0.0 0.0 1.3236416473799222 1.5501736969627446E-4 76-77 5.167245656542482E-5 0.0 0.0 1.4857898160822254 1.5501736969627446E-4 78-79 5.167245656542482E-5 0.0 0.0 1.6760219649278367 1.5501736969627446E-4 80-81 5.167245656542482E-5 0.0 0.0 1.8953457068197825 1.5501736969627446E-4 82-83 5.167245656542482E-5 0.0 0.0 2.1246163966005724 2.0668982626169928E-4 84-85 5.167245656542482E-5 0.0 0.0 2.3543004660338855 2.0668982626169928E-4 86-87 5.167245656542482E-5 0.0 0.0 2.599796307176219 2.0668982626169928E-4 88-89 5.167245656542482E-5 0.0 0.0 2.878750063944665 2.0668982626169928E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2825 0.0 55.849514 1 GTATCAA 5060 0.0 39.35168 1 ATCAACG 6130 0.0 32.092674 3 CAACGCA 6215 0.0 31.5259 5 TCAACGC 6280 0.0 31.400986 4 AACGCAG 6595 0.0 29.77979 6 TATCAAC 6650 0.0 29.65463 2 ACGCAGA 7560 0.0 25.664785 7 CGCAGAG 7695 0.0 25.152878 8 GTGGTAT 1375 0.0 24.884537 1 GTACATG 5665 0.0 24.075857 1 ACATGGG 5590 0.0 23.121881 3 TACATGG 5820 0.0 22.61637 2 GAGTACT 5455 0.0 21.08886 12-13 GCAGAGT 8710 0.0 21.077978 9 GGCGTCG 165 6.388348E-5 20.125664 8 GTACTTT 5950 0.0 19.573597 14-15 TGGTATC 1520 0.0 19.382696 2 AGAGTAC 8085 0.0 19.216196 10-11 CATGGGG 3215 0.0 19.213974 4 >>END_MODULE