FastQCFastQC Report
Sat 18 Jun 2016
SRR1930987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941745
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1333834.53414554966525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.054.602591
GTATCAA41200.046.8147851
ATCAACG48500.039.192243
TCAACGC50850.037.1798174
CAACGCA50550.036.8903965
TATCAAC51100.035.8282782
AACGCAG56600.033.051946
ACGCAGA63150.028.2600027
CGCAGAG62550.028.0417638
GTGTAGC9600.025.1141821
GTACATG40600.024.3850521
GCAGAGT68100.024.098439
TACATGG41900.022.2759382
ACATGGG41150.021.0745893
AGAGTAC58150.020.54101210-11
CAGAGTA64750.020.39093410-11
GAGTACT45500.020.26918812-13
TACGGCG1250.00427467920.1663344
CAACGCG1659.840466E-418.3273665
AGTACTT50650.018.01858312-13
Produced by FastQC (version 0.11.5)