##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930987_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2941745 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.608289637613048 27.0 2.0 31.0 2.0 31.0 2 18.237221785028954 25.0 2.0 31.0 2.0 31.0 3 18.72258064516129 25.0 2.0 31.0 2.0 31.0 4 20.185572848768334 26.0 2.0 35.0 2.0 35.0 5 19.605882562900593 26.0 2.0 35.0 2.0 35.0 6 20.460691188393284 26.0 2.0 35.0 2.0 36.0 7 20.482829069140934 26.0 2.0 35.0 2.0 36.0 8 20.702044194857134 27.0 2.0 35.0 2.0 35.0 9 21.700289793982822 28.0 2.0 35.0 2.0 38.0 10-11 22.08347443439183 29.0 2.0 35.0 2.0 38.0 12-13 21.938330140783783 29.5 2.0 35.0 2.0 39.0 14-15 21.769207052276794 27.0 2.0 36.0 2.0 39.0 16-17 20.71339494075795 26.5 2.0 36.0 2.0 38.5 18-19 19.83272938341019 24.5 2.0 36.0 2.0 38.5 20-21 19.451211270861343 21.5 2.0 35.0 2.0 39.0 22-23 19.06565694851185 18.0 2.0 34.0 2.0 39.0 24-25 18.866684569872643 18.0 2.0 34.0 2.0 38.0 26-27 18.54829837392432 18.0 2.0 34.0 2.0 38.0 28-29 18.28003735877855 17.0 2.0 34.0 2.0 38.0 30-31 18.15602168100906 17.0 2.0 34.0 2.0 38.0 32-33 17.98144774615067 16.0 2.0 34.0 2.0 38.0 34-35 17.61670641065082 15.5 2.0 34.0 2.0 38.0 36-37 17.15284805447107 14.5 2.0 33.0 2.0 38.0 38-39 16.95345687678572 9.0 2.0 33.0 2.0 38.0 40-41 16.74807316745673 9.0 2.0 33.0 2.0 38.0 42-43 16.55131512078715 9.0 2.0 33.0 2.0 37.0 44-45 16.145773511980135 9.0 2.0 32.0 2.0 37.0 46-47 15.790838260964156 8.0 2.0 31.5 2.0 37.0 48-49 15.471730214549527 7.5 2.0 31.0 2.0 37.0 50-51 13.606490365412366 4.0 2.0 29.0 2.0 35.5 52-53 15.153739192214145 2.0 2.0 30.5 2.0 36.5 54-55 15.796555955733757 2.0 2.0 33.0 2.0 37.5 56-57 15.718301892244229 2.0 2.0 33.0 2.0 38.0 58-59 15.409185704403338 2.0 2.0 32.0 2.0 37.5 60-61 15.082494913733175 2.0 2.0 31.5 2.0 37.0 62-63 14.619870349061525 2.0 2.0 31.0 2.0 37.0 64-65 14.224116638253825 2.0 2.0 31.0 2.0 36.5 66-67 13.823985763551907 2.0 2.0 30.0 2.0 36.0 68-69 13.360090864435904 2.0 2.0 29.0 2.0 35.5 70-71 12.971361215876971 2.0 2.0 29.0 2.0 35.0 72-73 12.545325648552135 2.0 2.0 28.0 2.0 34.0 74-75 12.079208938912108 2.0 2.0 28.0 2.0 34.0 76-77 11.636926722064626 2.0 2.0 26.0 2.0 34.0 78-79 11.217993401875418 2.0 2.0 26.0 2.0 33.5 80-81 10.756728404399428 2.0 2.0 25.5 2.0 33.0 82-83 10.283464236363113 2.0 2.0 24.0 2.0 32.0 84-85 9.854540927238766 2.0 2.0 21.5 2.0 32.0 86-87 9.367302570413138 2.0 2.0 19.0 2.0 31.5 88-89 8.929086137649593 2.0 2.0 14.5 2.0 31.0 90-91 8.45127500854085 2.0 2.0 6.0 2.0 31.0 92-93 7.933288235384101 2.0 2.0 2.0 2.0 31.0 94-95 7.400762812548335 2.0 2.0 2.0 2.0 30.0 96-97 6.84211598897933 2.0 2.0 2.0 2.0 29.5 98-99 6.287428040159837 2.0 2.0 2.0 2.0 29.0 100-101 5.632102204643842 2.0 2.0 2.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.724395464227619 1101 2 8.8142166567037 1101 3 8.102636923853147 1101 4 10.046952748087477 1101 5 10.923080729461336 1101 6 10.184965296490343 1101 7 10.109140692175853 1101 8 9.389484224752472 1101 9 9.15720685728073 1101 10-11 8.890943129474469 1101 12-13 8.724585911771587 1101 14-15 9.037254401363182 1101 16-17 9.813765634230695 1101 18-19 10.548330835945489 1101 20-21 10.337937047744077 1101 22-23 10.598472556661736 1101 24-25 10.49938951543988 1101 26-27 10.480988085633754 1101 28-29 10.649969800917972 1101 30-31 10.148296965387875 1101 32-33 9.941125842336245 1101 34-35 9.765701183344174 1101 36-37 10.22539028075802 1101 38-39 9.946348710064488 1101 40-41 9.64016482216072 1101 42-43 9.32120476520171 1101 44-45 9.289702322048736 1101 46-47 8.91385133116138 1101 48-49 8.980761997990454 1101 50-51 10.217227781458933 1101 52-53 9.786162373039334 1101 54-55 9.56502808921954 1101 56-57 9.40761429836917 1101 58-59 9.328804435587655 1101 60-61 9.0441739521099 1101 62-63 8.93965773564819 1101 64-65 8.765326310606714 1101 66-67 8.408962019691582 1101 68-69 8.264771120706333 1101 70-71 7.973765293970159 1101 72-73 7.742975499011301 1101 74-75 7.386053891426062 1101 76-77 7.195737718730696 1101 78-79 6.982964890930033 1101 80-81 6.793404744545031 1101 82-83 6.239830187065502 1101 84-85 5.7863454595160455 1101 86-87 5.653177869456213 1101 88-89 5.318060528596332 1101 90-91 5.0771934176796165 1101 92-93 4.841099808746031 1101 94-95 4.560402948226893 1101 96-97 3.9397254308751624 1101 98-99 3.469953960312429 1101 100-101 2.9368965545242105 1102 1 7.167522907757473 1102 2 7.973995354159225 1102 3 7.541198228411961 1102 4 9.219360431872968 1102 5 9.969726855980102 1102 6 9.139062643972878 1102 7 8.949962633814977 1102 8 8.364182760694678 1102 9 8.272751598484025 1102 10-11 7.66231342896997 1102 12-13 7.72470173623115 1102 14-15 7.953929461106625 1102 16-17 8.689381838667732 1102 18-19 9.61617627209554 1102 20-21 9.739056836787906 1102 22-23 9.79262822839782 1102 24-25 9.810243137128815 1102 26-27 9.853345182056472 1102 28-29 9.64664270898173 1102 30-31 9.39975598733501 1102 32-33 9.531727809063039 1102 34-35 9.226302097121334 1102 36-37 9.205586129389644 1102 38-39 8.867440664613689 1102 40-41 8.609732975470187 1102 42-43 8.181552513262908 1102 44-45 8.121789359780959 1102 46-47 8.229903319404974 1102 48-49 7.761149666576847 1102 50-51 9.43813501201828 1102 52-53 8.828149197434803 1102 54-55 8.772782147653666 1102 56-57 8.46967881213534 1102 58-59 8.193332851493606 1102 60-61 8.168415370570395 1102 62-63 8.251495178537112 1102 64-65 8.217210861546583 1102 66-67 7.750892305269836 1102 68-69 7.544247301154433 1102 70-71 7.237418846072854 1102 72-73 6.997221403952839 1102 74-75 6.560481865093166 1102 76-77 6.292475634989154 1102 78-79 6.144555806263726 1102 80-81 6.002781032119307 1102 82-83 5.790068474145352 1102 84-85 5.5074163550726 1102 86-87 5.2655188969519315 1102 88-89 5.081129922555148 1102 90-91 4.657325402627016 1102 92-93 4.316357781405963 1102 94-95 4.070476700481579 1102 96-97 3.8526117031587823 1102 98-99 3.3686441825305824 1102 100-101 2.776348974934951 1103 1 7.579172895071135 1103 2 8.699162914857176 1103 3 8.339470753397311 1103 4 9.97708663600849 1103 5 10.594544924917436 1103 6 9.878215376250736 1103 7 9.619686408460712 1103 8 9.139660519187228 1103 9 8.74107987599373 1103 10-11 8.534134547119063 1103 12-13 8.362331771191553 1103 14-15 8.641514644811533 1103 16-17 9.283464728727704 1103 18-19 9.928410055475762 1103 20-21 10.431475908058179 1103 22-23 10.405872484181334 1103 24-25 10.416638847574944 1103 26-27 10.307881670433318 1103 28-29 9.868565486234342 1103 30-31 9.692032533777109 1103 32-33 9.900965741886072 1103 34-35 9.751570699446187 1103 36-37 9.81713687137199 1103 38-39 9.833397607408411 1103 40-41 9.547605622603637 1103 42-43 8.955716506068747 1103 44-45 8.917081212906847 1103 46-47 8.926018100447768 1103 48-49 8.739367505588346 1103 50-51 9.877654918341465 1103 52-53 9.672261277298103 1103 54-55 9.382453122435924 1103 56-57 9.162801919008194 1103 58-59 8.926069384328768 1103 60-61 8.867025430723848 1103 62-63 8.672579198017939 1103 64-65 8.237081146551333 1103 66-67 8.085151647690928 1103 68-69 7.907940686969537 1103 70-71 7.3921130716561425 1103 72-73 7.089682239095566 1103 74-75 6.845532644124109 1103 76-77 6.6540808271815575 1103 78-79 6.115257832260612 1103 80-81 5.818870732384935 1103 82-83 5.5585402868995 1103 84-85 5.2929328237351 1103 86-87 5.0229032997173295 1103 88-89 4.636645111357041 1103 90-91 4.16268549253161 1103 92-93 3.8458695145842166 1103 94-95 3.3644108909472896 1103 96-97 2.9737589859248974 1103 98-99 2.630727638423668 1103 100-101 2.1495783888261535 1104 1 7.795099578123342 1104 2 9.049263546306346 1104 3 8.149702429648553 1104 4 9.991246056069272 1104 5 10.92516292143836 1104 6 10.190654621989047 1104 7 10.042288991510198 1104 8 9.523901533264215 1104 9 9.181167283100628 1104 10-11 8.810694367510369 1104 12-13 8.65516906875418 1104 14-15 9.056913704267505 1104 16-17 9.93273032111414 1104 18-19 10.49604798905374 1104 20-21 10.729143635921236 1104 22-23 11.057323528649722 1104 24-25 10.809537426265493 1104 26-27 10.688791023977238 1104 28-29 10.44218252824929 1104 30-31 10.261679748542708 1104 32-33 10.452398861926397 1104 34-35 10.28440555147142 1104 36-37 10.319045279630092 1104 38-39 9.865387607487438 1104 40-41 9.699263132774348 1104 42-43 9.307623520326622 1104 44-45 9.170224024827021 1104 46-47 8.686658987110937 1104 48-49 8.704928181259154 1104 50-51 10.224946035640327 1104 52-53 9.686889663181956 1104 54-55 9.434139138264738 1104 56-57 9.1416917064967 1104 58-59 9.293112881087223 1104 60-61 8.803181043737089 1104 62-63 8.778568273007686 1104 64-65 8.51298496360933 1104 66-67 8.257704782149823 1104 68-69 8.02093175922134 1104 70-71 7.718568989637118 1104 72-73 7.50862595573286 1104 74-75 7.347505813022494 1104 76-77 6.885359074894735 1104 78-79 6.714132511860091 1104 80-81 6.5782718369599795 1104 82-83 6.054410588679692 1104 84-85 5.650271170200574 1104 86-87 5.301403798923078 1104 88-89 4.964589865481745 1104 90-91 4.740456380801724 1104 92-93 4.358838052328475 1104 94-95 3.917196300967759 1104 96-97 3.5625828141524583 1104 98-99 3.070397119873146 1104 100-101 2.545108993607114 1105 1 7.824125814576508 1105 2 8.63513086862769 1105 3 8.278349423252838 1105 4 10.03908515033503 1105 5 10.15928742054351 1105 6 9.857475706283108 1105 7 9.540096774500817 1105 8 9.250046362455219 1105 9 9.014252521630947 1105 10-11 8.507447875913083 1105 12-13 8.354685198338377 1105 14-15 8.78561155730641 1105 16-17 9.667102059522978 1105 18-19 10.107139494972085 1105 20-21 10.22829604996922 1105 22-23 10.650945738438683 1105 24-25 10.64393778112661 1105 26-27 10.647389134350341 1105 28-29 10.324076141775762 1105 30-31 9.981408332828213 1105 32-33 10.042570629080014 1105 34-35 10.096809521672665 1105 36-37 9.939358212726624 1105 38-39 9.806274743173052 1105 40-41 9.502986565242946 1105 42-43 9.37027130783428 1105 44-45 9.071619386994104 1105 46-47 8.802497231010372 1105 48-49 8.588945357974515 1105 50-51 9.876107632618018 1105 52-53 9.333454448434514 1105 54-55 9.184939030129625 1105 56-57 9.038654512808234 1105 58-59 8.656325597571323 1105 60-61 8.58668646075879 1105 62-63 8.492769690056761 1105 64-65 8.367508587367574 1105 66-67 8.016697149384882 1105 68-69 7.77793188241324 1105 70-71 7.550345528081548 1105 72-73 7.253438207851932 1105 74-75 7.180845348725903 1105 76-77 6.813688763800963 1105 78-79 6.538576351751681 1105 80-81 6.149554392986628 1105 82-83 5.991219345228538 1105 84-85 5.498831963693304 1105 86-87 5.238097276938456 1105 88-89 4.8805928785503525 1105 90-91 4.588329138965754 1105 92-93 4.354168523161081 1105 94-95 4.076365371587516 1105 96-97 3.720897668700463 1105 98-99 3.2051585809975442 1105 100-101 2.688534951851903 1106 1 7.485061912377013 1106 2 8.645661439751485 1106 3 8.130579819974763 1106 4 9.663854025527634 1106 5 10.349926019232957 1106 6 9.66996999165875 1106 7 8.999509395777846 1106 8 8.776206642852788 1106 9 8.492676639885577 1106 10-11 8.145766972071161 1106 12-13 7.871819970346028 1106 14-15 8.335268256539383 1106 16-17 9.304997850173006 1106 18-19 10.200959022901781 1106 20-21 10.23564992490784 1106 22-23 10.309230250536753 1106 24-25 9.800220693129859 1106 26-27 10.102189207158887 1106 28-29 10.22044206398914 1106 30-31 9.963823798718256 1106 32-33 10.274358332901617 1106 34-35 9.9743823725506 1106 36-37 9.756055710144462 1106 38-39 9.550558637245086 1106 40-41 9.560729881248971 1106 42-43 9.331268149975191 1106 44-45 8.74492193190682 1106 46-47 8.819394951933516 1106 48-49 8.908455297187848 1106 50-51 10.40907657045984 1106 52-53 9.964408240405254 1106 54-55 9.726485407481087 1106 56-57 9.530266714166194 1106 58-59 9.083644888449937 1106 60-61 8.860119807073493 1106 62-63 8.756221492491616 1106 64-65 8.84593482214974 1106 66-67 8.303043006917179 1106 68-69 7.85806234517082 1106 70-71 7.509356220778683 1106 72-73 7.2582799762530215 1106 74-75 6.903056823691614 1106 76-77 6.761263223239828 1106 78-79 6.372778590191208 1106 80-81 6.086067879500119 1106 82-83 6.041555661454689 1106 84-85 5.717873515361974 1106 86-87 5.338072936871745 1106 88-89 4.922471550725634 1106 90-91 4.541637483163928 1106 92-93 4.157142667660648 1106 94-95 3.8616538119607027 1106 96-97 3.3352568264918254 1106 98-99 3.0283572044080334 1106 100-101 2.5398328065395894 1107 1 6.983498174252581 1107 2 7.613025626624342 1107 3 7.599854524076054 1107 4 9.11945483127295 1107 5 9.930259285837899 1107 6 9.115402016281525 1107 7 9.17260534286223 1107 8 7.491172152615988 1107 9 4.876571325803383 1107 10-11 6.92713984614635 1107 12-13 7.064829024554506 1107 14-15 7.281152157481085 1107 16-17 8.405168737410367 1107 18-19 9.561842736169886 1107 20-21 9.582714188518345 1107 22-23 9.178957665061859 1107 24-25 9.354817757914788 1107 26-27 9.318955028043206 1107 28-29 9.467357127089176 1107 30-31 9.29045947838862 1107 32-33 9.497095406361233 1107 34-35 9.650880424915709 1107 36-37 9.773539903856975 1107 38-39 9.42664891658681 1107 40-41 9.235701000367488 1107 42-43 8.802700996191273 1107 44-45 8.454340018637044 1107 46-47 8.608429287860917 1107 48-49 8.787007020031476 1107 50-51 9.84255055472862 1107 52-53 9.187702287398727 1107 54-55 9.115609409683122 1107 56-57 8.73999300264971 1107 58-59 8.75939766810445 1107 60-61 8.60397495641997 1107 62-63 8.387354665676813 1107 64-65 8.299333382484718 1107 66-67 8.429924539001021 1107 68-69 8.091298505285591 1107 70-71 7.969705272818485 1107 72-73 7.2860592137143385 1107 74-75 7.403065477529747 1107 76-77 7.095275934998204 1107 78-79 6.816107210831968 1107 80-81 6.501198333503401 1107 82-83 6.398108910710251 1107 84-85 6.272965100077837 1107 86-87 5.692110108755736 1107 88-89 5.518180393905576 1107 90-91 5.054362274429181 1107 92-93 4.600351802777298 1107 94-95 4.188002768078445 1107 96-97 3.762628707982202 1107 98-99 3.4351623533374607 1107 100-101 3.054165439224821 1108 1 6.656204250039352 1108 2 7.522590790400834 1108 3 7.653991248546191 1108 4 8.611123116436726 1108 5 10.24106108416802 1108 6 8.93004895269771 1108 7 8.324882902271352 1108 8 5.914438331164476 1108 9 6.729562873651808 1108 10-11 6.041002556486749 1108 12-13 4.924703346969405 1108 14-15 6.781924360435486 1108 16-17 7.991194993030149 1108 18-19 7.720804996927754 1108 20-21 7.434643950881867 1108 22-23 8.411690510277015 1108 24-25 8.422951861961032 1108 26-27 7.0138211552108345 1108 28-29 8.546782843209918 1108 30-31 8.938764577939036 1108 32-33 9.484067943291226 1108 34-35 8.851654580342807 1108 36-37 8.619849216637967 1108 38-39 8.37887422166067 1108 40-41 8.668317673456766 1108 42-43 7.854674124001164 1108 44-45 7.415352581257469 1108 46-47 8.244645601284168 1108 48-49 8.805683089395638 1108 50-51 9.754495012018277 1108 52-53 9.367807720924736 1108 54-55 8.984442550338228 1108 56-57 8.778586194685678 1108 58-59 8.727223589748638 1108 60-61 8.728152014865698 1108 62-63 8.650265648335772 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2313 92-93 -5.88246772194974 2313 94-95 -5.3662682659613745 2313 96-97 -4.8019184981835 2313 98-99 -4.257194743643915 2313 100-101 -3.60334901164223 2314 1 -17.45227574996065 2314 2 -16.078689209599165 2314 3 -16.56584875145381 2314 4 -18.020236883563275 2314 5 -17.41013891583198 2314 6 -18.26899104730229 2314 7 -18.29399709772865 2314 8 -18.535481668835523 2314 9 -19.52355712634819 2314 10-11 -19.907317443513254 2314 12-13 -19.789056653030595 2314 14-15 -19.635835639564515 2314 16-17 -18.56464500696985 2314 18-19 -17.687515003072246 2314 20-21 -17.300876049118134 2314 22-23 -16.931029489722984 2314 24-25 -16.74344813803897 2314 26-27 -16.412258844789164 2314 28-29 -16.15033715679008 2314 30-31 -16.031955422060964 2314 32-33 -15.840252056708774 2314 34-35 -15.49282541965719 2314 36-37 -15.024950783362033 2314 38-39 -14.827525778339332 2314 40-41 -14.634562326543236 2314 42-43 -14.432205875998836 2314 44-45 -14.030727418742533 2314 46-47 -13.693754398715834 2314 48-49 -13.361836910604362 2314 50-51 -11.53686498798172 2314 52-53 -13.055072279075263 2314 54-55 -13.69315744966177 2314 56-57 -13.614213805314323 2314 58-59 -13.290056410251362 2314 60-61 -12.982087985134301 2314 62-63 -12.512934351664228 2314 64-65 -12.128091151876234 2314 66-67 -11.73786608399191 2314 68-69 -11.280249343140868 2314 70-71 -10.886910012987551 2314 72-73 -10.462174569201522 2314 74-75 -9.99320941007462 2314 76-77 -9.55886664689004 2314 78-79 -9.14118244876983 2314 80-81 -8.686984068551832 2314 82-83 -8.214126156727131 2314 84-85 -7.780503108014649 2314 86-87 -7.309816673517867 2314 88-89 -6.870547929793846 2314 90-91 -6.395862516836072 2314 92-93 -5.88246772194974 2314 94-95 -5.3662682659613745 2314 96-97 -4.8019184981835 2314 98-99 -4.257194743643915 2314 100-101 -3.60334901164223 2315 1 -17.45227574996065 2315 2 -16.078689209599165 2315 3 -16.56584875145381 2315 4 -18.020236883563275 2315 5 -17.41013891583198 2315 6 -18.26899104730229 2315 7 -18.29399709772865 2315 8 -18.535481668835523 2315 9 -19.52355712634819 2315 10-11 -19.907317443513254 2315 12-13 -19.789056653030595 2315 14-15 -19.635835639564515 2315 16-17 -18.56464500696985 2315 18-19 -17.687515003072246 2315 20-21 -17.300876049118134 2315 22-23 -16.931029489722984 2315 24-25 -16.74344813803897 2315 26-27 -16.412258844789164 2315 28-29 -16.15033715679008 2315 30-31 -16.031955422060964 2315 32-33 -15.840252056708774 2315 34-35 -15.49282541965719 2315 36-37 -15.024950783362033 2315 38-39 -14.827525778339332 2315 40-41 -14.634562326543236 2315 42-43 -14.432205875998836 2315 44-45 -14.030727418742533 2315 46-47 -13.693754398715834 2315 48-49 -13.361836910604362 2315 50-51 -11.53686498798172 2315 52-53 -13.055072279075263 2315 54-55 -13.69315744966177 2315 56-57 -13.614213805314323 2315 58-59 -13.290056410251362 2315 60-61 -12.982087985134301 2315 62-63 -12.512934351664228 2315 64-65 -12.128091151876234 2315 66-67 -11.73786608399191 2315 68-69 -11.280249343140868 2315 70-71 -10.886910012987551 2315 72-73 -10.462174569201522 2315 74-75 -9.99320941007462 2315 76-77 -9.55886664689004 2315 78-79 -9.14118244876983 2315 80-81 -8.686984068551832 2315 82-83 -8.214126156727131 2315 84-85 -7.780503108014649 2315 86-87 -7.309816673517867 2315 88-89 -6.870547929793846 2315 90-91 -6.395862516836072 2315 92-93 -5.88246772194974 2315 94-95 -5.3662682659613745 2315 96-97 -4.8019184981835 2315 98-99 -4.257194743643915 2315 100-101 -3.60334901164223 2316 1 -17.45227574996065 2316 2 -16.078689209599165 2316 3 -16.56584875145381 2316 4 -18.020236883563275 2316 5 -17.41013891583198 2316 6 -18.26899104730229 2316 7 -18.29399709772865 2316 8 -18.535481668835523 2316 9 -19.52355712634819 2316 10-11 -19.907317443513254 2316 12-13 -19.789056653030595 2316 14-15 -19.635835639564515 2316 16-17 -18.56464500696985 2316 18-19 -17.687515003072246 2316 20-21 -17.300876049118134 2316 22-23 -16.931029489722984 2316 24-25 -16.74344813803897 2316 26-27 -16.412258844789164 2316 28-29 -16.15033715679008 2316 30-31 -16.031955422060964 2316 32-33 -15.840252056708774 2316 34-35 -15.49282541965719 2316 36-37 -15.024950783362033 2316 38-39 -14.827525778339332 2316 40-41 -14.634562326543236 2316 42-43 -14.432205875998836 2316 44-45 -14.030727418742533 2316 46-47 -13.693754398715834 2316 48-49 -13.361836910604362 2316 50-51 -11.53686498798172 2316 52-53 -13.055072279075263 2316 54-55 -13.69315744966177 2316 56-57 -13.614213805314323 2316 58-59 -13.290056410251362 2316 60-61 -12.982087985134301 2316 62-63 -12.512934351664228 2316 64-65 -12.128091151876234 2316 66-67 -11.73786608399191 2316 68-69 -11.280249343140868 2316 70-71 -10.886910012987551 2316 72-73 -10.462174569201522 2316 74-75 -9.99320941007462 2316 76-77 -9.55886664689004 2316 78-79 -9.14118244876983 2316 80-81 -8.686984068551832 2316 82-83 -8.214126156727131 2316 84-85 -7.780503108014649 2316 86-87 -7.309816673517867 2316 88-89 -6.870547929793846 2316 90-91 -6.395862516836072 2316 92-93 -5.88246772194974 2316 94-95 -5.3662682659613745 2316 96-97 -4.8019184981835 2316 98-99 -4.257194743643915 2316 100-101 -3.60334901164223 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 856011.0 3 41211.0 4 117041.0 5 69542.0 6 49070.0 7 48506.0 8 46702.0 9 48162.0 10 50980.0 11 51972.0 12 52210.0 13 52333.0 14 52223.0 15 53494.0 16 54208.0 17 54930.0 18 55364.0 19 56674.0 20 58753.0 21 61218.0 22 63252.0 23 66226.0 24 68641.0 25 71328.0 26 74179.0 27 77455.0 28 79881.0 29 83425.0 30 85611.0 31 85698.0 32 83224.0 33 73909.0 34 56061.0 35 31462.0 36 9749.0 37 1024.0 38 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.44428243694638 20.972258914454034 14.49082586753257 23.092632781067014 2 14.863081483273849 21.044824874478252 42.11108825842357 21.981005383824332 3 18.57749686236725 26.727520150179725 30.648361142418306 24.046621845034718 4 12.140726720565336 17.980050769985592 40.59475350958676 29.284468999862312 5 12.32241781373805 38.22985701019658 36.46567467903072 12.982050497034647 6 28.704118618402568 39.521150371938674 18.691305747977005 13.083425261681752 7 25.363233327015124 33.19662886695875 24.15229799022373 17.2878398158024 8 23.98213675213675 37.130085470085476 21.510299145299143 17.37747863247863 9 25.085113932141784 17.08585528135867 22.513684586862254 35.3153461996373 10-11 23.159382364232556 27.94652606639187 30.13952118869379 18.754570380681788 12-13 23.84562992006629 25.78414867063472 30.021827662520085 20.348393746778903 14-15 21.323243318676816 27.662376644763082 27.508656591826885 23.505723444733217 16-17 20.781869643067818 30.71795472254321 28.026404925139314 20.473770709249656 18-19 20.378764143385933 29.555385039669957 30.051470574440504 20.014380242503606 20-21 21.443363245136897 28.644218245250386 30.068461825626148 19.84395668398657 22-23 20.967062474181244 28.429331066086405 29.922735875339846 20.6808705843925 24-25 20.71795935130794 28.523459875914124 30.369114460534874 20.38946631224306 26-27 20.679976087297206 28.819054709067316 30.069167804823966 20.431801398811515 28-29 20.656211398467057 28.77035567859238 30.20743950648914 20.36599341645142 30-31 20.935766969749565 28.550479773024325 30.14927452556959 20.36447873165652 32-33 20.765221845188552 28.608885448375815 30.105873382651666 20.52001932378397 34-35 20.893478330347758 28.581392180778277 30.183130621752863 20.341998867121102 36-37 20.913106189270657 28.46419258332383 30.071187110789594 20.551514116615923 38-39 20.91039743021878 28.65125763306038 30.096428314536983 20.34191662218386 40-41 21.03411555010615 28.549118445102916 30.10174494510859 20.315021059682344 42-43 20.874347397354942 28.51385341816287 29.941285290628162 20.670513893854025 44-45 20.923210530033693 28.70107632590799 29.870430591298803 20.505282552759514 46-47 20.967433262500123 28.499592909874544 29.653857621941565 20.87911620568376 48-49 20.85023445065776 28.617778315172238 29.777507601481584 20.754479632688422 50-51 21.14070396842011 28.780214719339693 29.361547893178624 20.717533419061578 52-53 20.63818972396556 29.016010050065972 29.55314604463009 20.792654181338378 54-55 20.597807491205373 28.968297459804898 29.647252547501683 20.786642501488046 56-57 20.575551208386656 29.046908172881242 29.693429867372643 20.68411075135946 58-59 20.538500893627663 29.022652337754483 29.64959899111094 20.789247777506915 60-61 20.628224746334805 29.102199939405054 29.64103473425868 20.628540580001456 62-63 20.51058885913466 29.051087694848558 29.611669059158697 20.826654386858085 64-65 20.41371663692085 28.90095249150936 29.66159505416814 21.023735817401644 66-67 20.37831491596088 29.04323427635435 29.29848674922378 21.279964058460994 68-69 20.49553626916405 29.07815311785654 29.261237235224925 21.16507337775448 70-71 20.635074071008855 28.812138265883195 29.364892824629646 21.187894838478304 72-73 20.618434732491682 28.93675906483073 29.23720346344953 21.207602739228054 74-75 20.46997998687057 29.24679308261525 28.943331859621857 21.339895070892325 76-77 20.487550810726635 29.126014982525206 29.035273524279525 21.35116068246863 78-79 20.396712363403903 29.233767310542763 28.909112438998676 21.460407887054657 80-81 20.444274365620128 29.177722034053016 28.71734625542309 21.660657344903765 82-83 20.616192998408074 29.026439120070503 28.846074035572776 21.511293845948643 84-85 20.64712250369424 29.033442528375137 28.762695150400276 21.55673981753035 86-87 20.502302672170476 29.028359615128057 28.841869394166853 21.62746831853461 88-89 20.600212303254466 29.044616058504964 28.710402452100446 21.644769186140124 90-91 20.598443769520582 28.972889188672447 28.78188955431461 21.646777487492365 92-93 20.538353014455442 29.088522314079786 28.54398843338529 21.829136238079485 94-95 20.43223734195365 28.97729262125649 28.632936793918578 21.95753324287128 96-97 20.42444488944408 28.96087287737823 28.53820798647201 22.07647424670568 98-99 20.316462312098924 28.994126274065568 28.31189743410698 22.377513979728526 100-101 20.558443873223258 29.16943150606976 28.253351629590977 22.018772991116005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 181955.0 1 165231.0 2 109684.5 3 55604.5 4 34728.5 5 27277.5 6 25087.5 7 25232.0 8 25970.0 9 25590.0 10 23602.0 11 20141.5 12 16087.0 13 12478.0 14 9929.5 15 8379.5 16 7948.0 17 8561.0 18 9550.5 19 11081.5 20 12885.5 21 14747.0 22 16854.0 23 19185.0 24 21968.5 25 24992.0 26 28618.0 27 32981.0 28 37609.0 29 42919.5 30 48226.0 31 53670.5 32 59344.0 33 64653.0 34 69762.5 35 74320.5 36 79195.5 37 83135.5 38 84824.5 39 85227.5 40 84981.0 41 84629.0 42 83832.5 43 83100.5 44 82771.0 45 81929.0 46 79630.5 47 76815.0 48 74144.0 49 70772.0 50 66630.5 51 61573.0 52 56029.5 53 50781.5 54 45205.5 55 39357.0 56 33249.0 57 27220.0 58 22261.5 59 17945.0 60 14072.0 61 10671.0 62 7800.0 63 5648.5 64 4019.0 65 2745.0 66 1888.0 67 1268.0 68 842.0 69 568.0 70 359.0 71 226.0 72 151.5 73 111.0 74 79.5 75 50.0 76 39.0 77 30.5 78 16.0 79 9.5 80 9.0 81 6.5 82 3.5 83 2.5 84 2.5 85 3.0 86 2.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.667362398848304 2 29.75682800514661 3 23.51124927551504 4 29.88392943643994 5 33.04718797856374 6 25.58141511245876 7 24.497432646269477 8 20.455375975823873 9 12.144764417038187 10-11 15.8370117056373 12-13 15.902805987602598 14-15 20.10264315907735 16-17 23.500728309217827 18-19 27.522881826942854 20-21 29.61174404987516 22-23 29.231799493157972 24-25 27.701636273708292 26-27 29.177698950792813 28-29 26.096398566157163 30-31 28.022058336123628 32-33 26.995286811059422 34-35 25.014421712283013 36-37 28.03500983259936 38-39 28.16590153123401 40-41 25.143919680325794 42-43 26.02057622261617 44-45 24.689138589510648 46-47 28.06177965799211 48-49 23.389943723878172 50-51 28.95585783268094 52-53 22.55637385293423 54-55 23.699980793712573 56-57 25.83476813931867 58-59 25.118628569097595 60-61 24.65844252306029 62-63 24.482475537478603 64-65 22.98501399679442 66-67 23.50387270140682 68-69 24.51541517024759 70-71 22.985761852233964 72-73 22.852881537998705 74-75 20.074428612949117 76-77 22.39777750960739 78-79 21.4859377682294 80-81 22.539785059547988 82-83 23.415166848248234 84-85 20.335957059500398 86-87 21.90215331376445 88-89 20.725912681078746 90-91 21.859457566852328 92-93 21.848069768113824 94-95 20.874192018682788 96-97 19.790719453929555 98-99 19.188049949944674 100-101 20.851569391636595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2941745.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.78221746644108 #Duplication Level Percentage of deduplicated Percentage of total 1 92.70470295565949 76.74300880237227 2 5.036225296042294 8.338197953339295 3 1.1288742677399521 2.8035214537295445 4 0.4166826866406668 1.379756671199544 5 0.20438147197686124 0.8459575729649931 6 0.12538861082218328 0.6227968349338154 7 0.08585508199838396 0.49750918480125456 8 0.05373794747561544 0.3558837163301268 9 0.03876781121716848 0.2888356840989817 >10 0.1963614925062718 2.7466992561942947 >50 0.006649118779780689 0.38120699638548905 >100 0.0022106576742361534 0.3015663276578245 >500 0.0 0.0 >1k 1.2195110041038462E-4 0.20655152624552942 >5k 0.0 0.0 >10k+ 4.0650366803461545E-5 4.488508019747059 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 133383 4.53414554966525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.399342907016074E-5 0.0 4 0.0 0.0 0.0 6.798685814032148E-5 0.0 5 0.0 0.0 0.0 6.798685814032148E-5 0.0 6 0.0 0.0 0.0 6.798685814032148E-5 0.0 7 0.0 0.0 0.0 6.798685814032148E-5 0.0 8 0.0 0.0 0.0 6.798685814032148E-5 0.0 9 0.0 0.0 0.0 1.0198028721048221E-4 0.0 10-11 0.0 0.0 0.0 1.0198028721048221E-4 0.0 12-13 0.0 0.0 0.0 1.0198028721048221E-4 0.0 14-15 0.0 0.0 0.0 1.0198028721048221E-4 0.0 16-17 0.0 0.0 0.0 1.3597371628064296E-4 0.0 18-19 0.0 0.0 0.0 1.3597371628064296E-4 0.0 20-21 0.0 0.0 0.0 1.3597371628064296E-4 0.0 22-23 0.0 0.0 0.0 1.3597371628064296E-4 0.0 24-25 0.0 0.0 0.0 2.3795400349112516E-4 0.0 26-27 0.0 3.399342907016074E-5 0.0 4.0792114884192885E-4 0.0 28-29 0.0 3.399342907016074E-5 0.0 5.608915796576522E-4 0.0 30-31 0.0 3.399342907016074E-5 0.0 0.0013427404482713491 0.0 32-33 0.0 3.399342907016074E-5 0.0 0.0034673297651563947 0.0 34-35 0.0 3.399342907016074E-5 0.0 0.00642475809426038 0.0 36-37 0.0 3.399342907016074E-5 0.0 0.011455785596644168 0.0 38-39 0.0 3.399342907016074E-5 0.0 0.019104307137430332 0.0 40-41 0.0 6.798685814032148E-5 0.0 0.027806624979391487 0.0 42-43 0.0 6.798685814032148E-5 0.0 0.0367129033957736 0.0 44-45 0.0 6.798685814032148E-5 0.0 0.048916544431961306 0.0 46-47 0.0 6.798685814032148E-5 0.0 0.06400962693911266 0.0 48-49 0.0 6.798685814032148E-5 0.0 0.08115931190500876 1.699671453508037E-5 50-51 0.0 6.798685814032148E-5 0.0 0.10135140877268425 3.399342907016074E-5 52-53 0.0 6.798685814032148E-5 0.0 0.12356611467003428 3.399342907016074E-5 54-55 0.0 6.798685814032148E-5 0.0 0.14997900905754918 6.798685814032148E-5 56-57 0.0 6.798685814032148E-5 0.0 0.1796382759212644 6.798685814032148E-5 58-59 0.0 6.798685814032148E-5 0.0 0.21347873456060945 6.798685814032148E-5 60-61 0.0 6.798685814032148E-5 0.0 0.24884889750811168 6.798685814032148E-5 62-63 0.0 6.798685814032148E-5 0.0 0.2869045413521566 6.798685814032148E-5 64-65 0.0 6.798685814032148E-5 0.0 0.3242803166147984 6.798685814032148E-5 66-67 0.0 6.798685814032148E-5 0.0 0.36740098139029725 6.798685814032148E-5 68-69 0.0 6.798685814032148E-5 0.0 0.41857808885542425 6.798685814032148E-5 70-71 0.0 6.798685814032148E-5 0.0 0.477318734288662 6.798685814032148E-5 72-73 0.0 6.798685814032148E-5 0.0 0.5382179624678549 6.798685814032148E-5 74-75 0.0 6.798685814032148E-5 0.0 0.6000350132319423 8.498357267540185E-5 76-77 0.0 6.798685814032148E-5 0.0 0.6681918385176145 1.0198028721048221E-4 78-79 0.0 6.798685814032148E-5 0.0 0.7476684756836504 1.0198028721048221E-4 80-81 0.0 6.798685814032148E-5 0.0 0.8369522171364275 1.0198028721048221E-4 82-83 0.0 6.798685814032148E-5 0.0 0.9294143442072647 1.0198028721048221E-4 84-85 0.0 6.798685814032148E-5 0.0 1.0200578228228483 1.0198028721048221E-4 86-87 0.0 6.798685814032148E-5 0.0 1.1157153322262805 1.0198028721048221E-4 88-89 0.0 6.798685814032148E-5 0.0 1.2248682329705667 1.0198028721048221E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2095 0.0 54.60259 1 GTATCAA 4120 0.0 46.814785 1 ATCAACG 4850 0.0 39.19224 3 TCAACGC 5085 0.0 37.179817 4 CAACGCA 5055 0.0 36.890396 5 TATCAAC 5110 0.0 35.828278 2 AACGCAG 5660 0.0 33.05194 6 ACGCAGA 6315 0.0 28.260002 7 CGCAGAG 6255 0.0 28.041763 8 GTGTAGC 960 0.0 25.114182 1 GTACATG 4060 0.0 24.385052 1 GCAGAGT 6810 0.0 24.09843 9 TACATGG 4190 0.0 22.275938 2 ACATGGG 4115 0.0 21.074589 3 AGAGTAC 5815 0.0 20.541012 10-11 CAGAGTA 6475 0.0 20.390934 10-11 GAGTACT 4550 0.0 20.269188 12-13 TACGGCG 125 0.004274679 20.166334 4 CAACGCG 165 9.840466E-4 18.327366 5 AGTACTT 5065 0.0 18.018583 12-13 >>END_MODULE