FastQCFastQC Report
Sat 18 Jun 2016
SRR1930987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941745
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71970.2446507090179468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60460.2055242721581918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.12948097132824227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.056.0852131
GTATCAA73150.044.0464441
ATCAACG90250.035.066543
CAACGCA91800.034.3682755
TCAACGC92100.034.3621644
TATCAAC96500.033.1412122
AACGCAG96500.032.791046
ACGCAGA109050.028.6692567
CGCAGAG109150.028.5995248
GCAGAGT127350.024.0275789
GTACATG72000.021.9789641
AGAGTAC116150.021.81078510-11
ACATGGG68950.021.7091183
TACATGG72850.021.5260852
CAGAGTA122200.020.22626910-11
GAGTACT92150.020.18087612-13
AGTACTT102700.019.00853712-13
TATACCG3003.778041E-918.9774915
GTGGTAT19650.018.6218851
GTACTTT102450.018.31402414-15
Produced by FastQC (version 0.11.5)