FastQCFastQC Report
Sat 18 Jun 2016
SRR1930986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704579
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN762424.472775975768797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.056.0533831
GTATCAA27450.048.8948251
ATCAACG32250.040.2991333
TCAACGC33200.039.744154
TATCAAC33700.038.754032
CAACGCA33700.038.697615
AACGCAG37250.036.042056
ACGCAGA42000.030.7284667
CGCAGAG43400.030.45588
GCAGAGT47200.026.681939
GTACATG28700.023.2041381
GAGTACT28750.023.06259512-13
AGAGTAC40800.021.72331210-11
CAGAGTA43450.021.61072510-11
TACATGG31150.020.8810482
GTACTTT32400.020.68918414-15
ACATGGG30500.020.1495673
ATACCGT2109.96899E-720.0482776
GATACCG2059.612808E-619.649455
AGTACTT31850.019.15848712-13
Produced by FastQC (version 0.11.5)