FastQCFastQC Report
Sat 18 Jun 2016
SRR1930986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704579
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42760.2508537298652629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.2124864849326432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22880.13422669175203966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.054.7030751
GTATCAA44750.041.755991
ATCAACG54300.033.9683573
TCAACGC54800.033.6584324
CAACGCA56000.032.957175
AACGCAG58300.031.7374176
TATCAAC59100.031.4535922
ACGCAGA65400.028.0742917
CGCAGAG65950.027.768228
GTACATG43250.023.9656751
GCAGAGT76500.023.566649
ATACCGT2554.984031E-1022.3262946
TACATGG45200.022.2986412
ACATGGG42550.022.2329033
AGAGTAC70850.021.16034710-11
TATACCG1805.223539E-621.0865615
GAGTACT54100.020.91542412-13
CAGAGTA75800.019.77850310-11
AGTACTT57300.019.74737412-13
ATGGGCG2001.2982893E-518.9779055
Produced by FastQC (version 0.11.5)