FastQCFastQC Report
Sat 18 Jun 2016
SRR1930985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619934
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1176734.491449021234886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.11034629116611334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.060.800641
GTATCAA46850.048.7003671
ATCAACG59200.038.476913
TATCAAC61700.036.5561372
TCAACGC62400.036.335944
CAACGCA62600.036.1352045
AACGCAG69400.032.7032436
ACGCAGA78950.028.1490127
CGCAGAG79800.027.2766368
GTACATG55700.027.1549241
TACATGG58500.024.623132
GCAGAGT84500.024.3551839
ACATGGG58850.024.2339063
GTGGTAT11850.022.4991841
GTACTAG8800.022.1402591
TGGTATC12350.021.1687892
GAGTACT53300.020.68324312-13
GAGTACA42000.020.6309491
AGAGTAC76650.020.43150110-11
CAGAGTA81700.020.40166910-11
Produced by FastQC (version 0.11.5)