##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930985_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2619934 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.514595405838467 28.0 2.0 31.0 2.0 33.0 2 19.306224889634624 27.0 2.0 31.0 2.0 33.0 3 19.896456933647947 27.0 2.0 31.0 2.0 33.0 4 21.42386525767443 28.0 2.0 35.0 2.0 37.0 5 20.797310924626345 28.0 2.0 35.0 2.0 37.0 6 21.705698693173186 29.0 2.0 35.0 2.0 37.0 7 21.847815631996838 29.0 2.0 35.0 2.0 37.0 8 22.221321605811443 30.0 2.0 35.0 2.0 37.0 9 23.41169166856875 32.0 2.0 37.0 2.0 39.0 10-11 23.700696086237286 32.0 2.0 37.0 2.0 39.0 12-13 23.56297257869855 32.0 2.0 37.0 2.0 39.0 14-15 23.48630461683386 31.5 2.0 38.0 2.0 40.0 16-17 22.398736380382104 29.5 2.0 37.0 2.0 40.0 18-19 21.424156677229277 27.0 2.0 37.0 2.0 39.5 20-21 21.10591717195929 27.0 2.0 37.0 2.0 39.5 22-23 20.744086873944152 26.0 2.0 37.0 2.0 39.0 24-25 20.563909816048803 25.5 2.0 36.5 2.0 39.0 26-27 20.269088839642524 25.0 2.0 36.0 2.0 39.0 28-29 20.041890559075153 24.0 2.0 36.0 2.0 39.0 30-31 19.97320734033758 24.0 2.0 36.0 2.0 39.0 32-33 19.805771824786426 24.0 2.0 36.0 2.0 39.0 34-35 19.460594045498855 23.5 2.0 35.0 2.0 39.0 36-37 19.015663753361725 20.0 2.0 35.0 2.0 38.5 38-39 18.8442479848729 19.0 2.0 35.0 2.0 38.0 40-41 18.65458252001768 19.0 2.0 35.0 2.0 38.0 42-43 18.47747767691858 18.5 2.0 35.0 2.0 38.0 44-45 18.06264508953279 15.5 2.0 34.0 2.0 38.0 46-47 17.685883499355327 15.0 2.0 34.0 2.0 38.0 48-49 17.396929082946365 12.5 2.0 33.5 2.0 38.0 50-51 15.316983366756567 7.0 2.0 31.0 2.0 37.5 52-53 17.103045343890344 12.5 2.0 33.5 2.0 37.5 54-55 17.784051239458705 13.5 2.0 34.5 2.0 38.0 56-57 17.71176926594334 11.0 2.0 34.5 2.0 39.0 58-59 17.42594107332475 8.5 2.0 34.0 2.0 38.0 60-61 17.118921316338504 8.0 2.0 34.0 2.0 38.0 62-63 16.675882865751582 6.5 2.0 34.0 2.0 38.0 64-65 16.31220671971126 2.0 2.0 33.0 2.0 38.0 66-67 15.929002028295368 2.0 2.0 32.5 2.0 37.5 68-69 15.48416868516535 2.0 2.0 32.0 2.0 37.0 70-71 15.118807382170697 2.0 2.0 31.0 2.0 36.0 72-73 14.713178652592013 2.0 2.0 31.0 2.0 36.0 74-75 14.253256761429867 2.0 2.0 31.0 2.0 35.0 76-77 13.83284998782412 2.0 2.0 30.0 2.0 35.0 78-79 13.443041885787963 2.0 2.0 29.5 2.0 34.5 80-81 13.005762358899117 2.0 2.0 29.0 2.0 34.0 82-83 12.54346788888575 2.0 2.0 29.0 2.0 34.0 84-85 12.131268001407669 2.0 2.0 28.5 2.0 34.0 86-87 11.65784901451716 2.0 2.0 26.5 2.0 34.0 88-89 11.23851135181268 2.0 2.0 26.0 2.0 33.5 90-91 10.754568626537921 2.0 2.0 25.5 2.0 33.0 92-93 10.223257532441657 2.0 2.0 24.5 2.0 32.5 94-95 9.673097108553115 2.0 2.0 21.0 2.0 32.0 96-97 9.065450885403983 2.0 2.0 12.5 2.0 32.0 98-99 8.428608888620857 2.0 2.0 2.0 2.0 32.0 100-101 7.597474211182419 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.163774444597873 1101 2 9.761296492712194 1101 3 9.01217396179667 1101 4 11.05648050583022 1101 5 11.85797294552258 1101 6 11.027357515543581 1101 7 10.845087756640957 1101 8 10.226239144472714 1101 9 9.968699556011366 1101 10-11 9.764242101526335 1101 12-13 9.687774192068137 1101 14-15 10.263931000536754 1101 16-17 11.275261115426478 1101 18-19 12.16314783505048 1101 20-21 11.904691425971972 1101 22-23 12.21704792119294 1101 24-25 12.09289538888634 1101 26-27 12.128263458295582 1101 28-29 12.100866785901665 1101 30-31 11.722418566853495 1101 32-33 11.55681486810683 1101 34-35 11.595009721056297 1101 36-37 11.808358834745395 1101 38-39 11.627081031503728 1101 40-41 11.407604946453333 1101 42-43 11.123937399490295 1101 44-45 10.897535431105794 1101 46-47 10.676703121310396 1101 48-49 10.943179553347814 1101 50-51 12.3145343344813 1101 52-53 11.616609297827981 1101 54-55 11.26554976738867 1101 56-57 11.434296009405593 1101 58-59 11.2977867843526 1101 60-61 10.965175723196289 1101 62-63 11.066518546383406 1101 64-65 10.999480171587987 1101 66-67 10.5305329948996 1101 68-69 10.605521569432304 1101 70-71 10.252169410337277 1101 72-73 10.15634888242994 1101 74-75 10.142883676790406 1101 76-77 9.799837435730206 1101 78-79 9.384245351523647 1101 80-81 9.199273027693371 1101 82-83 8.867838920127067 1101 84-85 8.448660083076005 1101 86-87 8.35408527793255 1101 88-89 8.064740163094976 1101 90-91 7.818967173440717 1101 92-93 7.300300763947687 1101 94-95 7.001546236815932 1101 96-97 6.691676505090674 1101 98-99 6.303562456277117 1101 100-101 5.744309633749146 1102 1 7.7488978282390875 1102 2 8.735983146185323 1102 3 8.44689000999118 1102 4 10.294574741376977 1102 5 10.779021250313356 1102 6 9.93498269875932 1102 7 9.820677706292972 1102 8 9.420379649636843 1102 9 9.332576531282506 1102 10-11 8.621141339388984 1102 12-13 8.836120789117832 1102 14-15 9.239792640826362 1102 16-17 9.891711131367 1102 18-19 10.735450127477833 1102 20-21 10.783114878388016 1102 22-23 11.139300286706078 1102 24-25 10.907021397199472 1102 26-27 11.182870268807907 1102 28-29 10.556446870476652 1102 30-31 10.714977150773823 1102 32-33 10.53427156302531 1102 34-35 10.388940894098166 1102 36-37 10.525961480671338 1102 38-39 10.448424386311647 1102 40-41 10.373252121572484 1102 42-43 9.88309969003285 1102 44-45 9.893995432429161 1102 46-47 9.497932686866037 1102 48-49 9.400839926015134 1102 50-51 11.15541986550115 1102 52-53 10.376421239784431 1102 54-55 10.083380693603104 1102 56-57 10.010913771757494 1102 58-59 10.077914869946177 1102 60-61 9.937959539657733 1102 62-63 9.894133844471146 1102 64-65 9.644076736054863 1102 66-67 9.612080789393206 1102 68-69 9.417411393364743 1102 70-71 8.938127616096317 1102 72-73 8.780271393452958 1102 74-75 8.331882612083922 1102 76-77 8.124882199519758 1102 78-79 7.886687058342384 1102 80-81 7.681988590654415 1102 82-83 7.747700027060894 1102 84-85 7.549451795609267 1102 86-87 7.078224038662329 1102 88-89 6.747660145690736 1102 90-91 6.666980824140799 1102 92-93 6.425463928712523 1102 94-95 5.973929535160321 1102 96-97 5.5532626570890535 1102 98-99 5.094876977790808 1102 100-101 4.52424823768034 1103 1 8.048431735233947 1103 2 9.219486326022569 1103 3 8.738112921583266 1103 4 10.836239801500795 1103 5 11.239749799334145 1103 6 10.571907228126083 1103 7 10.198263751330934 1103 8 9.673545936041805 1103 9 9.697662974463697 1103 10-11 9.13631939742234 1103 12-13 8.986466098040655 1103 14-15 9.57336192640933 1103 16-17 10.563760345222715 1103 18-19 11.221481377111118 1103 20-21 11.662955461839669 1103 22-23 11.756196814729496 1103 24-25 11.607529260318671 1103 26-27 11.43691791858939 1103 28-29 11.114390749883679 1103 30-31 10.941586934647706 1103 32-33 11.06269158541074 1103 34-35 10.944928004315948 1103 36-37 10.958229215088291 1103 38-39 10.623435087284012 1103 40-41 10.386540186719337 1103 42-43 10.173055109167308 1103 44-45 10.277720217389906 1103 46-47 9.933295251456045 1103 48-49 9.82278199044784 1103 50-51 11.458363624766713 1103 52-53 10.847559278416341 1103 54-55 10.415255774977993 1103 56-57 10.154918684705223 1103 58-59 10.025540106247675 1103 60-61 9.933494049036312 1103 62-63 9.736653739059584 1103 64-65 9.744683618093266 1103 66-67 9.505997621883035 1103 68-69 9.265908675094243 1103 70-71 8.99203935235283 1103 72-73 8.892453905661286 1103 74-75 8.568466652156408 1103 76-77 8.243719012205803 1103 78-79 7.795037909977731 1103 80-81 7.319365326908139 1103 82-83 7.192331086591267 1103 84-85 6.951310398045509 1103 86-87 6.788924787506874 1103 88-89 6.451167983105245 1103 90-91 6.1633781867283 1103 92-93 5.529987422515736 1103 94-95 5.2337564085150134 1103 96-97 4.869161508033729 1103 98-99 4.520712952839379 1103 100-101 3.981629449902136 1104 1 8.008097766192947 1104 2 9.515043174461102 1104 3 8.83352171562375 1104 4 10.839977523785912 1104 5 11.646294259613992 1104 6 11.005800776579708 1104 7 10.397341830487935 1104 8 10.014256662279017 1104 9 9.776612113870371 1104 10-11 9.185101639143944 1104 12-13 9.136014065386195 1104 14-15 9.865144961589618 1104 16-17 10.51315252439024 1104 18-19 11.386335309073402 1104 20-21 11.492207846828872 1104 22-23 11.545115637718741 1104 24-25 11.591833214695338 1104 26-27 11.506769147799087 1104 28-29 11.478712163607803 1104 30-31 11.163328381087073 1104 32-33 11.294769567963332 1104 34-35 11.148575933345356 1104 36-37 11.254557541997613 1104 38-39 11.01631070205168 1104 40-41 10.779673187111179 1104 42-43 10.160411871421687 1104 44-45 10.309701025675743 1104 46-47 10.204736952865925 1104 48-49 10.026475236714187 1104 50-51 11.51809676322688 1104 52-53 10.911718038536737 1104 54-55 10.677238385098882 1104 56-57 10.552690596267688 1104 58-59 10.492853488780511 1104 60-61 10.06894482800434 1104 62-63 9.934664420902187 1104 64-65 9.509352020744785 1104 66-67 9.40992684341431 1104 68-69 9.295093789765849 1104 70-71 8.885221333351069 1104 72-73 8.68992031934742 1104 74-75 8.415251651988166 1104 76-77 8.176619109135844 1104 78-79 7.998712703716462 1104 80-81 7.820871107433776 1104 82-83 7.483086071758692 1104 84-85 7.162075304160135 1104 86-87 6.945959867932759 1104 88-89 6.675662112487855 1104 90-91 6.347780943535922 1104 92-93 5.7455175582069415 1104 94-95 5.553717267447611 1104 96-97 5.011531984857896 1104 98-99 4.562906556759131 1104 100-101 3.901412513303934 1105 1 7.962340545862279 1105 2 9.101918306436527 1105 3 8.512896219018614 1105 4 10.657037663777228 1105 5 10.558124344420044 1105 6 10.564483176350585 1105 7 10.025247442460454 1105 8 9.711626983261791 1105 9 9.48822981925592 1105 10-11 9.121109422680142 1105 12-13 8.729744103482549 1105 14-15 9.602600440997218 1105 16-17 10.512460224707414 1105 18-19 10.856288628521881 1105 20-21 11.283775104153715 1105 22-23 11.587485146646966 1105 24-25 11.400075011950886 1105 26-27 11.54204556560261 1105 28-29 11.37568065668626 1105 30-31 10.970301955798877 1105 32-33 10.871162109036998 1105 34-35 10.920644211103511 1105 36-37 10.898962168244541 1105 38-39 10.615677829394816 1105 40-41 10.42733689426073 1105 42-43 9.995904523586532 1105 44-45 9.88483104050998 1105 46-47 10.051761375704451 1105 48-49 9.711035827773319 1105 50-51 11.169773450531833 1105 52-53 10.41845424957236 1105 54-55 10.310280805079714 1105 56-57 10.088080940157553 1105 58-59 9.902042210521873 1105 60-61 9.682459124535924 1105 62-63 9.773619829967046 1105 64-65 9.446857799844228 1105 66-67 9.277913666140288 1105 68-69 9.17240400546502 1105 70-71 8.933255816922795 1105 72-73 8.755150488458773 1105 74-75 8.45963619545057 1105 76-77 8.061288088943636 1105 78-79 7.848344179321574 1105 80-81 7.6898945913093915 1105 82-83 7.5155826930554 1105 84-85 7.280118692512703 1105 86-87 6.840484249042541 1105 88-89 6.552019654408019 1105 90-91 6.08042748361393 1105 92-93 5.799340348034242 1105 94-95 5.61452134186837 1105 96-97 5.331321979339105 1105 98-99 5.160342239522141 1105 100-101 4.543573259302547 1106 1 7.880527432853846 1106 2 9.124502210891865 1106 3 8.578328088870627 1106 4 10.470664636187642 1106 5 10.926696095493345 1106 6 10.292118672313382 1106 7 9.387334735935326 1106 8 8.974212553353254 1106 9 9.152612390730965 1106 10-11 8.700068366059249 1106 12-13 8.347046461745691 1106 14-15 8.926878056445315 1106 16-17 9.898033707675626 1106 18-19 10.97901032770119 1106 20-21 10.996538119489355 1106 22-23 11.05815391002476 1106 24-25 10.758609603101384 1106 26-27 11.17819894397621 1106 28-29 10.961316483157358 1106 30-31 10.671409904994878 1106 32-33 10.70670849922902 1106 34-35 10.502943854851186 1106 36-37 10.84964827414181 1106 38-39 10.461550377852124 1106 40-41 10.322111510909075 1106 42-43 9.987039856259372 1106 44-45 9.640519017628957 1106 46-47 9.59237127351139 1106 48-49 9.443193028205652 1106 50-51 10.861772648617086 1106 52-53 10.311474579499258 1106 54-55 10.04542600219439 1106 56-57 9.834634173337967 1106 58-59 9.440719276414807 1106 60-61 9.151573294201917 1106 62-63 9.20809007780375 1106 64-65 8.926139682830144 1106 66-67 8.706790872878988 1106 68-69 8.537201427709801 1106 70-71 8.339084718380775 1106 72-73 8.12484562728928 1106 74-75 7.507094646864671 1106 76-77 7.43844546091554 1106 78-79 7.246478679962033 1106 80-81 6.727129546307099 1106 82-83 6.598296388800776 1106 84-85 6.37884285714774 1106 86-87 6.061693100170027 1106 88-89 5.756466077886577 1106 90-91 5.616133575835169 1106 92-93 5.397027418481484 1106 94-95 5.061014221928689 1106 96-97 4.396735516718101 1106 98-99 4.007917612830216 1106 100-101 3.6234063342789566 1107 1 7.025421496800021 1107 2 7.932565019821649 1107 3 7.934738969282968 1107 4 9.239671934672018 1107 5 10.197429539743048 1107 6 9.223185162075456 1107 7 9.34673248032379 1107 8 7.958837532000683 1107 9 5.217138496248154 1107 10-11 7.586265010956044 1107 12-13 7.564600693376825 1107 14-15 8.015451642281086 1107 16-17 9.006438664748185 1107 18-19 10.209645593022973 1107 20-21 10.42394288702128 1107 22-23 9.407448241667833 1107 24-25 9.720484245762687 1107 26-27 9.950542118602414 1107 28-29 10.016045365951829 1107 30-31 9.730623217767342 1107 32-33 10.021514625681483 1107 34-35 10.04896849357348 1107 36-37 10.10459622066795 1107 38-39 9.934474342418167 1107 40-41 9.373068301104755 1107 42-43 9.25415135841179 1107 44-45 8.644809421958907 1107 46-47 9.016559895301384 1107 48-49 9.063337219487252 1107 50-51 10.563908958752359 1107 52-53 9.44637681007271 1107 54-55 9.43893010149808 1107 56-57 9.092732160385243 1107 58-59 9.051391506060053 1107 60-61 8.885986892143332 1107 62-63 8.901696279448466 1107 64-65 8.643958725345747 1107 66-67 8.62946492461032 1107 68-69 8.093901182086979 1107 70-71 7.911292874556018 1107 72-73 7.587453472399483 1107 74-75 7.6137350430435955 1107 76-77 7.422371557228702 1107 78-79 7.224925452974256 1107 80-81 6.702145672860963 1107 82-83 6.784478135647809 1107 84-85 6.538087019023239 1107 86-87 6.047030416196664 1107 88-89 5.691426925655806 1107 90-91 5.5674803283333265 1107 92-93 5.027755855114462 1107 94-95 4.728263940542748 1107 96-97 4.31932805817719 1107 98-99 4.0869905719338995 1107 100-101 3.6786964289478723 1108 1 6.513716777706257 1108 2 7.8217664297543585 1108 3 7.684879564752556 1108 4 8.902630078952221 1108 5 10.685515389949789 1108 6 9.433227383616476 1108 7 8.859616953357932 1108 8 5.778028248097673 1108 9 6.849516638391147 1108 10-11 5.95294813567768 1108 12-13 4.764433070816967 1108 14-15 7.078425932615215 1108 16-17 8.45292931602696 1108 18-19 7.923034805742944 1108 20-21 8.07519182719274 1108 22-23 8.995338935222748 1108 24-25 8.633879581503109 1108 26-27 7.299689224753749 1108 28-29 9.064268247016248 1108 30-31 9.522561812842252 1108 32-33 9.831618506428445 1108 34-35 9.332857461762632 1108 36-37 8.91102336413461 1108 38-39 8.720189686707418 1108 40-41 8.985358451153857 1108 42-43 8.31605353516506 1108 44-45 7.911937304166035 1108 46-47 8.62386875371297 1108 48-49 8.861299579681535 1108 50-51 10.602773368559488 1108 52-53 9.980121087778596 1108 54-55 9.406297132503965 1108 56-57 9.2622799616749 1108 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5.272995876381408 1109 20-21 7.325240440667006 1109 22-23 8.708222740531184 1109 24-25 8.259143579675861 1109 26-27 8.707396543859538 1109 28-29 9.055003521956312 1109 30-31 6.6444083995204615 1109 32-33 5.52874170763344 1109 34-35 7.087784205886766 1109 36-37 5.547319341485359 1109 38-39 5.965756022107978 1109 40-41 6.812144205399626 1109 42-43 5.723026250659718 1109 44-45 6.468057316172793 1109 46-47 6.051696263846566 1109 48-49 6.482581516997932 1109 50-51 8.318363323935406 1109 52-53 8.950892236376095 1109 54-55 8.951072071409175 1109 56-57 8.1210205871837 1109 58-59 7.752299623298956 1109 60-61 7.831859257176966 1109 62-63 8.203187254595523 1109 64-65 8.198440921239108 1109 66-67 7.707125379880489 1109 68-69 7.415370852612403 1109 70-71 7.0883867677859556 1109 72-73 6.544313548955225 1109 74-75 5.730268304226103 1109 76-77 5.476382876083099 1109 78-79 6.346069748046363 1109 80-81 6.37239123726893 1109 82-83 6.10791163244302 1109 84-85 5.655890661169076 1109 86-87 5.601851109394241 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58-59 -15.220547822217302 2313 60-61 -14.91412145021853 2313 62-63 -14.469195739330878 2313 64-65 -14.089519064470071 2313 66-67 -13.724814598683286 2313 68-69 -13.296311891224684 2313 70-71 -12.932513553757452 2313 72-73 -12.53750138494974 2313 74-75 -12.080378355295155 2313 76-77 -11.671705726989428 2313 78-79 -11.288978483464893 2313 80-81 -10.863943167875766 2313 82-83 -10.40584328002572 2313 84-85 -10.004166502103521 2313 86-87 -9.538198908469282 2313 88-89 -9.122043641335887 2313 90-91 -8.637106164985562 2313 92-93 -8.1015326967093 2313 94-95 -7.562815871663824 2313 96-97 -6.954236405528119 2313 98-99 -6.320196138069711 2313 100-101 -5.498623903302037 2314 1 -18.25410179680778 2314 2 -17.04504422539323 2314 3 -17.63297500688171 2314 4 -19.127247530838996 2314 5 -18.480071363109968 2314 6 -19.38822690004083 2314 7 -19.565149065360572 2314 8 -19.96207254383897 2314 9 -21.144363851169686 2314 10-11 -21.453099380521024 2314 12-13 -21.309361025655317 2314 14-15 -21.213150741250875 2314 16-17 -20.121224751647627 2314 18-19 -19.146439636301693 2314 20-21 -18.834995357832458 2314 22-23 -18.463624347714617 2314 24-25 -18.289266566805026 2314 26-27 -18.001606671574546 2314 28-29 -17.76582534549635 2314 30-31 -17.71321932466675 2314 32-33 -17.537531961534118 2314 34-35 -17.212858880929954 2314 36-37 -16.75600327373436 2314 38-39 -16.596944942522246 2314 40-41 -16.411149828183795 2314 42-43 -16.249821194856537 2314 44-45 -15.846162047885802 2314 46-47 -15.471883586099842 2314 48-49 -15.187836489432936 2314 50-51 -13.16216543633612 2314 52-53 -14.915667249154449 2314 54-55 -15.57822410579697 2314 56-57 -15.517957619318622 2314 58-59 -15.220547822217302 2314 60-61 -14.91412145021853 2314 62-63 -14.469195739330878 2314 64-65 -14.089519064470071 2314 66-67 -13.724814598683286 2314 68-69 -13.296311891224684 2314 70-71 -12.932513553757452 2314 72-73 -12.53750138494974 2314 74-75 -12.080378355295155 2314 76-77 -11.671705726989428 2314 78-79 -11.288978483464893 2314 80-81 -10.863943167875766 2314 82-83 -10.40584328002572 2314 84-85 -10.004166502103521 2314 86-87 -9.538198908469282 2314 88-89 -9.122043641335887 2314 90-91 -8.637106164985562 2314 92-93 -8.1015326967093 2314 94-95 -7.562815871663824 2314 96-97 -6.954236405528119 2314 98-99 -6.320196138069711 2314 100-101 -5.498623903302037 2315 1 -18.25410179680778 2315 2 -17.04504422539323 2315 3 -17.63297500688171 2315 4 -19.127247530838996 2315 5 -18.480071363109968 2315 6 -19.38822690004083 2315 7 -19.565149065360572 2315 8 -19.96207254383897 2315 9 -21.144363851169686 2315 10-11 -21.453099380521024 2315 12-13 -21.309361025655317 2315 14-15 -21.213150741250875 2315 16-17 -20.121224751647627 2315 18-19 -19.146439636301693 2315 20-21 -18.834995357832458 2315 22-23 -18.463624347714617 2315 24-25 -18.289266566805026 2315 26-27 -18.001606671574546 2315 28-29 -17.76582534549635 2315 30-31 -17.71321932466675 2315 32-33 -17.537531961534118 2315 34-35 -17.212858880929954 2315 36-37 -16.75600327373436 2315 38-39 -16.596944942522246 2315 40-41 -16.411149828183795 2315 42-43 -16.249821194856537 2315 44-45 -15.846162047885802 2315 46-47 -15.471883586099842 2315 48-49 -15.187836489432936 2315 50-51 -13.16216543633612 2315 52-53 -14.915667249154449 2315 54-55 -15.57822410579697 2315 56-57 -15.517957619318622 2315 58-59 -15.220547822217302 2315 60-61 -14.91412145021853 2315 62-63 -14.469195739330878 2315 64-65 -14.089519064470071 2315 66-67 -13.724814598683286 2315 68-69 -13.296311891224684 2315 70-71 -12.932513553757452 2315 72-73 -12.53750138494974 2315 74-75 -12.080378355295155 2315 76-77 -11.671705726989428 2315 78-79 -11.288978483464893 2315 80-81 -10.863943167875766 2315 82-83 -10.40584328002572 2315 84-85 -10.004166502103521 2315 86-87 -9.538198908469282 2315 88-89 -9.122043641335887 2315 90-91 -8.637106164985562 2315 92-93 -8.1015326967093 2315 94-95 -7.562815871663824 2315 96-97 -6.954236405528119 2315 98-99 -6.320196138069711 2315 100-101 -5.498623903302037 2316 1 -18.25410179680778 2316 2 -17.04504422539323 2316 3 -17.63297500688171 2316 4 -19.127247530838996 2316 5 -18.480071363109968 2316 6 -19.38822690004083 2316 7 -19.565149065360572 2316 8 -19.96207254383897 2316 9 -21.144363851169686 2316 10-11 -21.453099380521024 2316 12-13 -21.309361025655317 2316 14-15 -21.213150741250875 2316 16-17 -20.121224751647627 2316 18-19 -19.146439636301693 2316 20-21 -18.834995357832458 2316 22-23 -18.463624347714617 2316 24-25 -18.289266566805026 2316 26-27 -18.001606671574546 2316 28-29 -17.76582534549635 2316 30-31 -17.71321932466675 2316 32-33 -17.537531961534118 2316 34-35 -17.212858880929954 2316 36-37 -16.75600327373436 2316 38-39 -16.596944942522246 2316 40-41 -16.411149828183795 2316 42-43 -16.249821194856537 2316 44-45 -15.846162047885802 2316 46-47 -15.471883586099842 2316 48-49 -15.187836489432936 2316 50-51 -13.16216543633612 2316 52-53 -14.915667249154449 2316 54-55 -15.57822410579697 2316 56-57 -15.517957619318622 2316 58-59 -15.220547822217302 2316 60-61 -14.91412145021853 2316 62-63 -14.469195739330878 2316 64-65 -14.089519064470071 2316 66-67 -13.724814598683286 2316 68-69 -13.296311891224684 2316 70-71 -12.932513553757452 2316 72-73 -12.53750138494974 2316 74-75 -12.080378355295155 2316 76-77 -11.671705726989428 2316 78-79 -11.288978483464893 2316 80-81 -10.863943167875766 2316 82-83 -10.40584328002572 2316 84-85 -10.004166502103521 2316 86-87 -9.538198908469282 2316 88-89 -9.122043641335887 2316 90-91 -8.637106164985562 2316 92-93 -8.1015326967093 2316 94-95 -7.562815871663824 2316 96-97 -6.954236405528119 2316 98-99 -6.320196138069711 2316 100-101 -5.498623903302037 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 712043.0 3 30570.0 4 98853.0 5 55726.0 6 35734.0 7 35172.0 8 34178.0 9 34465.0 10 37072.0 11 37931.0 12 39597.0 13 39751.0 14 40318.0 15 41438.0 16 42862.0 17 42978.0 18 41646.0 19 42643.0 20 44185.0 21 46723.0 22 49388.0 23 51953.0 24 54909.0 25 58326.0 26 62230.0 27 65969.0 28 70085.0 29 74321.0 30 79791.0 31 85311.0 32 89992.0 33 94252.0 34 93242.0 35 81704.0 36 53908.0 37 18898.0 38 1755.0 39 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50143051026946 21.23852565421117 14.470543889157353 22.789499946362017 2 14.81289051612397 20.997502320433618 41.83085594910471 22.35875121433771 3 18.528639889893622 26.820877251079732 30.042423573425303 24.608059285601342 4 11.953068584229118 17.634194313669788 39.813310896233475 30.59942620586762 5 12.419537667094438 38.428742115457275 35.56409584336099 13.587624374087298 6 28.902912194758066 39.29914807650451 18.24630674763874 13.551632981098683 7 25.960059586691635 33.299278037810446 23.204301042767103 17.536361332730817 8 24.491755009269518 36.6908783751466 20.75400674598847 18.063359869595413 9 25.632645167109562 16.790790414009866 21.676065206380486 35.90049921250009 10-11 23.880430535504157 27.56273853812232 28.933043823931403 19.62378710244212 12-13 24.545496132657743 25.116029326307483 29.307533538329423 21.03094100270535 14-15 22.009573823797233 26.527144496981137 27.111928254454437 24.351353424767193 16-17 20.94465103188187 29.678302086638098 27.903426478354397 21.473620403125633 18-19 20.66093698274537 28.37305761459149 30.49278184688397 20.473223555779168 20-21 21.58611064384967 27.688449090118215 30.341426061524245 20.38401420450787 22-23 21.31130507902577 27.558729348175987 30.132218389221492 20.997747183576752 24-25 21.025616888145382 27.865061231938267 30.461722445890732 20.647599434025622 26-27 20.865696313947744 28.025187308129922 30.25788411584122 20.85123226208111 28-29 20.850541133922768 27.95278396505291 30.402533242113016 20.79414165891131 30-31 21.22291550114227 27.804882041230456 30.275129322054884 20.69707313557239 32-33 21.03843576152112 28.052106913375418 30.076065407814628 20.83339191728883 34-35 21.10795832094818 27.932999432903564 30.377727820087387 20.58131442606087 36-37 21.21444800675342 27.829909343374716 30.085574656082226 20.870067993789643 38-39 21.221645475181358 28.043663454873514 30.108497230026828 20.6261938399183 40-41 21.285431835196384 27.942517999007737 30.20457176447815 20.56747840131773 42-43 21.1034466450869 28.006709363551174 29.788093706964485 21.10175028439744 44-45 21.19861900452443 28.16666510910233 29.698601197775393 20.936114688597847 46-47 21.26503145182446 28.081746476447258 29.392893005149112 21.26032906657917 48-49 21.33825607456404 28.316246734198785 29.202990585158094 21.14250660607908 50-51 21.576297424143988 28.427650935065152 28.738728103671328 21.25732353711953 52-53 20.99511538194246 28.891763140595028 28.72298339141455 21.39013808604796 54-55 20.963717419347123 28.78491834525274 28.88109270420665 21.370271531193488 56-57 20.990168253078718 28.816778188498514 28.903274796442002 21.289778761980767 58-59 20.96021881000225 28.79841376967363 29.01088616324319 21.230481257080932 60-61 21.107618168204507 29.02652379388951 28.872180413178945 20.99367762472704 62-63 20.986626284316856 28.848317407686814 28.935516491529878 21.22953981646645 64-65 20.796182693834766 28.62218889480846 28.997838575608732 21.58378983574805 66-67 20.727935236965312 28.655221272292998 28.7740625647194 21.84278092602229 68-69 20.81500460443194 28.771478113331494 28.773241298429657 21.64027598380691 70-71 20.99594696007189 28.430125497801857 28.92931639615533 21.644611145970927 72-73 20.990865240729775 28.760094606661024 28.74863116665278 21.500408985956422 74-75 20.941415556412103 29.03814549058606 28.392812630868224 21.62762632213361 76-77 20.83218666733976 28.81452584636203 28.62744877006747 21.72583871623074 78-79 20.693045867455893 28.93329711578606 28.55194669720442 21.821710319553624 80-81 20.84385895987821 28.907282816873742 28.271521877915827 21.977336345332223 82-83 21.076354220492778 28.674678930474272 28.60202376663141 21.646943082401535 84-85 21.074487015813386 28.791675451085375 28.243957493839368 21.889880039261875 86-87 20.864175582242073 28.795307480803 28.454547004342313 21.88596993261262 88-89 21.133948871340333 28.68548862029029 28.294077866501816 21.88648464186756 90-91 21.02472818664671 28.623646532362233 28.42950283145503 21.922122449536026 92-93 21.030724636834563 28.803412999354755 28.208430904798725 21.957431459011953 94-95 20.87967285478062 28.547205057506975 28.307477457310092 22.265644630402317 96-97 20.826735984289694 28.611093981984375 28.256876817334465 22.305293216391465 98-99 20.795143536192167 28.732523687268024 27.96956352331411 22.502769253225694 100-101 21.036352363553927 28.751285866324714 27.880875468519324 22.331486301602034 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 159469.0 1 144558.5 2 95949.0 3 48723.5 4 30156.5 5 23617.5 6 21941.5 7 22278.5 8 23003.5 9 22805.5 10 21128.0 11 18180.0 12 14776.0 13 11378.5 14 8672.0 15 7114.5 16 6556.5 17 6911.0 18 7864.5 19 9149.0 20 10663.0 21 12358.5 22 14074.0 23 15989.0 24 17922.5 25 19700.0 26 22243.0 27 25286.5 28 28546.5 29 32395.5 30 36705.0 31 41376.0 32 46909.5 33 53158.0 34 58625.5 35 63232.5 36 68336.0 37 73257.5 38 75699.0 39 75859.0 40 75107.0 41 73852.5 42 72729.0 43 73106.5 44 73907.5 45 74387.5 46 73534.5 47 71728.0 48 70840.5 49 69420.0 50 66441.5 51 62012.5 52 57022.0 53 51846.5 54 46379.0 55 40531.5 56 34177.5 57 28114.0 58 22867.0 59 18070.5 60 13679.0 61 10131.0 62 7406.5 63 5191.0 64 3598.0 65 2452.0 66 1594.5 67 1014.0 68 697.0 69 470.5 70 266.5 71 173.5 72 117.5 73 69.0 74 46.5 75 28.0 76 22.5 77 20.0 78 14.0 79 9.0 80 5.5 81 6.5 82 6.5 83 3.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.435374326223485 2 29.47475012729328 3 23.23676855981868 4 29.595020332573263 5 32.72250369665801 6 25.295141022636447 7 24.234465448366258 8 20.1993638007675 9 11.98129418527337 10-11 15.648810237204449 12-13 15.728907674773488 14-15 19.897256953801126 16-17 23.242875583888754 18-19 27.246373381924887 20-21 29.31858207115141 22-23 28.916701718440237 24-25 27.421854901688363 26-27 28.88217794799411 28-29 25.827120072490377 30-31 27.71503022595226 32-33 26.703458942095487 34-35 24.752073907205297 36-37 27.74753104467517 38-39 27.864213373313984 40-41 24.874691499862212 42-43 25.74847305313798 44-45 24.441359973190163 46-47 27.759382488261153 48-49 23.125773397345124 50-51 28.70566586791881 52-53 22.28485526734643 54-55 23.423567158562008 56-57 25.556693412887498 58-59 24.837915764290244 60-61 24.37609115344127 62-63 24.20854876496889 64-65 22.732423793881832 66-67 23.23907777829518 68-69 24.233053199050055 70-71 22.716507362399206 72-73 22.586332327455576 74-75 19.839335647386537 76-77 22.122637440485143 78-79 21.2401342934593 80-81 22.276668038202487 82-83 23.142281446784537 84-85 20.06884524571993 86-87 21.63714811136464 88-89 20.47551579543607 90-91 21.605238910598512 92-93 21.591020995185374 94-95 20.629928082157793 96-97 19.533469163727023 98-99 18.9381297391461 100-101 20.615423899991374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2619934.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.0305048385311 #Duplication Level Percentage of deduplicated Percentage of total 1 88.2504089086143 64.44971914802905 2 7.398055406987466 10.805674423914388 3 1.9939186985705133 4.368506674907746 4 0.8542719133541241 2.4955163640651836 5 0.3897781128051895 1.4232846176586456 6 0.24949106355130624 1.0932274994312363 7 0.1567682000191823 0.8014202553020093 8 0.12322746361542089 0.7199491102244728 9 0.08968770376926014 0.5894944455669927 >10 0.46152910069241354 5.993489217908935 >50 0.022850157092398442 1.1176465788222785 >100 0.009633521642640272 1.1608968746051609 >500 1.627496939200857E-4 0.06719378052146324 >1k 1.627496939088139E-4 0.25189743899099115 >5k 0.0 0.0 >10k+ 5.4249897969604634E-5 4.662083570051416 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 117673 4.491449021234886 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.11034629116611334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.816890043794996E-5 0.0 8 0.0 0.0 0.0 3.816890043794996E-5 0.0 9 0.0 0.0 0.0 3.816890043794996E-5 0.0 10-11 0.0 0.0 0.0 5.725335065692494E-5 0.0 12-13 0.0 0.0 0.0 9.542225109487491E-5 0.0 14-15 0.0 3.816890043794996E-5 0.0 1.145067013138499E-4 0.0 16-17 0.0 3.816890043794996E-5 0.0 1.3359115153282488E-4 0.0 18-19 0.0 7.633780087589992E-5 0.0 1.9084450218974982E-4 0.0 20-21 0.0 1.145067013138499E-4 0.0 2.4809785284667476E-4 0.0 22-23 0.0 1.145067013138499E-4 0.0 3.8168900437949964E-4 0.0 24-25 0.0 1.145067013138499E-4 0.0 4.198579048174496E-4 0.0 26-27 0.0 1.145067013138499E-4 0.0 4.389423550364246E-4 0.0 28-29 0.0 1.145067013138499E-4 0.0 8.015469091969493E-4 0.0 30-31 0.0 1.5267560175179985E-4 0.0 0.001965698372554423 0.0 32-33 0.0 1.5267560175179985E-4 0.0 0.004790197004962721 0.0 34-35 0.0 1.5267560175179985E-4 0.0 0.008988776053137217 0.0 36-37 0.0 1.7176005197077482E-4 0.0 0.016030938183938984 0.0 38-39 0.0 1.9084450218974982E-4 0.0 0.02828315522452092 0.0 40-41 0.0 2.0992895240872482E-4 0.0 0.04055445671532183 0.0 42-43 3.816890043794996E-5 2.290134026276998E-4 0.0 0.052520407002619146 0.0 44-45 3.816890043794996E-5 2.290134026276998E-4 0.0 0.06759712267560938 0.0 46-47 3.816890043794996E-5 2.290134026276998E-4 0.0 0.08692967074743105 0.0 48-49 3.816890043794996E-5 2.290134026276998E-4 0.0 0.10836150834333995 0.0 50-51 3.816890043794996E-5 2.290134026276998E-4 0.0 0.13275143572318998 0.0 52-53 3.816890043794996E-5 2.290134026276998E-4 0.0 0.16172163115559401 0.0 54-55 3.816890043794996E-5 2.290134026276998E-4 0.0 0.1935926630212822 1.908445021897498E-5 56-57 3.816890043794996E-5 2.290134026276998E-4 0.0 0.231895154610765 3.816890043794996E-5 58-59 3.816890043794996E-5 2.290134026276998E-4 0.0 0.27441530969864125 3.816890043794996E-5 60-61 3.816890043794996E-5 2.290134026276998E-4 0.0 0.3218592529430131 3.816890043794996E-5 62-63 3.816890043794996E-5 2.290134026276998E-4 0.0 0.3701238275468008 3.816890043794996E-5 64-65 3.816890043794996E-5 2.290134026276998E-4 0.0 0.42121290078299684 3.816890043794996E-5 66-67 3.816890043794996E-5 2.290134026276998E-4 0.0 0.4809663144186075 3.816890043794996E-5 68-69 3.816890043794996E-5 2.290134026276998E-4 0.0 0.5511016689733406 3.816890043794996E-5 70-71 3.816890043794996E-5 2.290134026276998E-4 0.0 0.6278974966544959 3.816890043794996E-5 72-73 3.816890043794996E-5 2.290134026276998E-4 0.0 0.7087010588816359 3.816890043794996E-5 74-75 3.816890043794996E-5 2.290134026276998E-4 0.0 0.7905733503210386 3.816890043794996E-5 76-77 3.816890043794996E-5 2.290134026276998E-4 0.0 0.8808809687572282 3.816890043794996E-5 78-79 3.816890043794996E-5 2.290134026276998E-4 0.0 0.9869905119747291 3.816890043794996E-5 80-81 3.816890043794996E-5 2.290134026276998E-4 0.0 1.099932288370623 3.816890043794996E-5 82-83 3.816890043794996E-5 2.290134026276998E-4 0.0 1.2213666451139609 3.816890043794996E-5 84-85 3.816890043794996E-5 2.290134026276998E-4 0.0 1.3382207338047447 3.816890043794996E-5 86-87 3.816890043794996E-5 2.290134026276998E-4 0.0 1.464521625353921 3.816890043794996E-5 88-89 3.816890043794996E-5 2.290134026276998E-4 0.0 1.605822894775212 3.816890043794996E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2260 0.0 60.80064 1 GTATCAA 4685 0.0 48.700367 1 ATCAACG 5920 0.0 38.47691 3 TATCAAC 6170 0.0 36.556137 2 TCAACGC 6240 0.0 36.33594 4 CAACGCA 6260 0.0 36.135204 5 AACGCAG 6940 0.0 32.703243 6 ACGCAGA 7895 0.0 28.149012 7 CGCAGAG 7980 0.0 27.276636 8 GTACATG 5570 0.0 27.154924 1 TACATGG 5850 0.0 24.62313 2 GCAGAGT 8450 0.0 24.355183 9 ACATGGG 5885 0.0 24.233906 3 GTGGTAT 1185 0.0 22.499184 1 GTACTAG 880 0.0 22.140259 1 TGGTATC 1235 0.0 21.168789 2 GAGTACT 5330 0.0 20.683243 12-13 GAGTACA 4200 0.0 20.630949 1 AGAGTAC 7665 0.0 20.431501 10-11 CAGAGTA 8170 0.0 20.401669 10-11 >>END_MODULE