FastQCFastQC Report
Sat 18 Jun 2016
SRR1930985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619934
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105470.40256739291905824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87110.33248929171498215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55760.212829788842009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.058.662471
GTATCAA94400.043.1497271
GTGGTAT21050.033.4178771
ATCAACG120850.033.113223
TATCAAC124400.032.246532
CAACGCA124150.032.1376235
TCAACGC124900.031.9615574
AACGCAG129300.031.150526
ACGCAGA146150.027.1041367
CGCAGAG146450.027.048618
TGGTATC24200.025.532242
GCAGAGT162550.023.5523629
GTACATG95250.023.35341
TACATGG99300.022.1611672
ACATGGG95100.022.0890873
GAGTACT106000.021.88521812-13
AGAGTAC151700.021.17146910-11
CAGAGTA157650.020.52287910-11
AGTACTT117050.019.73810612-13
GTACTTT116850.019.73128914-15
Produced by FastQC (version 0.11.5)