##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930985_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2619934 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.341470052299027 31.0 31.0 34.0 30.0 34.0 2 31.491089851881764 31.0 31.0 34.0 30.0 34.0 3 31.547907695384694 31.0 31.0 34.0 30.0 34.0 4 35.33149919043762 37.0 35.0 37.0 33.0 37.0 5 35.203646351396635 37.0 35.0 37.0 33.0 37.0 6 35.239410992796 37.0 35.0 37.0 32.0 37.0 7 35.122015287408004 37.0 35.0 37.0 32.0 37.0 8 35.12723984649995 37.0 35.0 37.0 32.0 37.0 9 36.61875871682264 39.0 35.0 39.0 32.0 39.0 10-11 36.55983337748202 38.0 35.0 39.0 32.0 39.0 12-13 36.44455451931232 38.0 35.0 39.0 32.0 39.0 14-15 37.48182110694391 39.0 36.0 41.0 32.0 41.0 16-17 37.4419559805705 39.0 36.0 41.0 32.0 41.0 18-19 37.49091637422927 39.0 36.0 41.0 32.0 41.0 20-21 37.488037294069244 39.0 36.0 41.0 32.0 41.0 22-23 37.38460281823893 39.0 36.0 41.0 32.0 41.0 24-25 37.25950252945303 39.0 36.0 40.5 31.0 41.0 26-27 36.99862668296224 39.0 36.0 40.0 31.0 41.0 28-29 36.813447781509 39.0 35.5 40.0 30.5 41.0 30-31 36.57156248974211 38.0 35.0 40.0 30.0 41.0 32-33 36.38385432610134 38.0 35.0 40.0 30.0 41.0 34-35 36.44577630581534 38.0 35.0 40.0 30.0 41.0 36-37 36.496296471590504 38.0 35.0 40.0 30.0 41.0 38-39 36.46994561695065 38.5 35.0 40.0 30.0 41.0 40-41 36.344501235527304 38.5 35.0 40.0 30.0 41.0 42-43 36.1776802392732 38.0 35.0 40.0 30.0 41.0 44-45 35.964236885356655 38.0 35.0 40.0 29.5 41.0 46-47 35.666350564556204 38.0 35.0 40.0 28.0 41.0 48-49 35.39864382079854 38.0 34.0 40.0 27.0 41.0 50-51 35.11237630413591 38.0 34.0 40.0 26.5 41.0 52-53 34.90745682906516 38.0 34.0 40.0 26.0 41.0 54-55 34.74891161380401 38.0 34.0 40.0 26.0 41.0 56-57 34.56818816809889 38.0 33.0 40.0 25.5 41.0 58-59 34.35529998083921 38.0 33.0 40.0 25.0 41.0 60-61 34.041622040860574 37.0 33.0 40.0 24.0 41.0 62-63 33.78367069552134 37.0 33.0 40.0 23.0 41.0 64-65 33.45838005842896 36.5 32.0 39.0 23.0 41.0 66-67 33.1355066578013 36.0 32.0 39.0 22.0 40.5 68-69 32.79202949387275 36.0 31.0 39.0 22.0 40.0 70-71 32.420356772346175 35.0 31.0 38.5 21.5 40.0 72-73 31.99947804028651 35.0 31.0 38.0 20.5 40.0 74-75 31.48562196604953 35.0 30.5 37.0 19.0 39.0 76-77 30.117375475870766 33.5 28.5 35.5 18.0 38.5 78-79 30.843589571340345 34.0 30.0 36.0 18.5 39.0 80-81 30.816149948815504 34.0 30.0 36.0 18.0 38.0 82-83 30.552963547936706 34.0 30.0 35.5 18.0 37.0 84-85 30.21740719422703 34.0 30.0 35.0 17.0 37.0 86-87 29.8853581807786 34.0 30.0 35.0 12.0 36.0 88-89 29.539012051448623 34.0 29.0 35.0 7.5 36.0 90-91 29.202004134455294 34.0 29.0 35.0 4.5 36.0 92-93 28.88715001980966 34.0 29.0 35.0 2.0 35.0 94-95 28.504008116234992 34.0 29.0 35.0 2.0 35.0 96-97 28.082875751831917 34.0 29.0 35.0 2.0 35.0 98-99 27.589267325054756 33.0 27.5 35.0 2.0 35.0 100-101 26.46301109111909 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3775349365046985 1101 2 0.48137916271627645 1101 3 0.4148796727756441 1101 4 0.3199587146471643 1101 5 0.36482370120423013 1101 6 0.4171394560460513 1101 7 0.4980090605372993 1101 8 0.47458476527337723 1101 9 0.5970562356946303 1101 10-11 0.5481185665080304 1101 12-13 0.542050487503694 1101 14-15 0.6426951403174996 1101 16-17 0.7445816139607757 1101 18-19 0.6319626994856051 1101 20-21 0.6199910030711422 1101 22-23 0.6360388609092098 1101 24-25 0.6846790582558526 1101 26-27 0.6463364895375818 1101 28-29 0.6428360000134603 1101 30-31 0.6691275008809896 1101 32-33 0.776422765104023 1101 34-35 0.7634998501676691 1101 36-37 0.753924808009252 1101 38-39 0.7749585559313772 1101 40-41 0.8033743121149897 1101 42-43 0.7843174416289358 1101 44-45 0.8109833067768122 1101 46-47 0.9413665964085212 1101 48-49 0.8175642410301194 1101 50-51 0.8094071376847083 1101 52-53 0.6361120915555816 1101 54-55 0.8385123552734584 1101 56-57 0.9333951600527968 1101 58-59 0.9089487497081734 1101 60-61 0.8573142720353886 1101 62-63 0.606086025707171 1101 64-65 0.7471400965339114 1101 66-67 0.7493953127314654 1101 68-69 0.7603262804845556 1101 70-71 0.6827462966164362 1101 72-73 0.7396738939193703 1101 74-75 0.9023337035256702 1101 76-77 0.8408500593873143 1101 78-79 0.9340429264909602 1101 80-81 0.9095512618094865 1101 82-83 0.9499518578249457 1101 84-85 0.9267296301771708 1101 86-87 1.0052072948495088 1101 88-89 0.9524414475041212 1101 90-91 0.8774419334363124 1101 92-93 0.8743627832787197 1101 94-95 1.0052053915527814 1101 96-97 1.21180354054189 1101 98-99 1.272535405655102 1101 100-101 1.4364063083092162 1102 1 0.34358262974090437 1102 2 0.43108030301491596 1102 3 0.4026826679894775 1102 4 0.150397690523846 1102 5 0.22371199524768315 1102 6 0.2976537233134451 1102 7 0.40254238424616773 1102 8 0.29647100008225635 1102 9 0.5143550091768248 1102 10-11 0.5253266294039136 1102 12-13 0.4989754908622288 1102 14-15 0.6139911748372455 1102 16-17 0.5149503913930076 1102 18-19 0.612786954742468 1102 20-21 0.5431551864252313 1102 22-23 0.470780774854461 1102 24-25 0.5908620754856457 1102 26-27 0.5512618097014439 1102 28-29 0.6723941367971591 1102 30-31 0.6182461605137703 1102 32-33 0.7156893808412192 1102 34-35 0.6736309033047405 1102 36-37 0.7481426096610448 1102 38-39 0.7678148967497336 1102 40-41 0.7685743506128446 1102 42-43 0.7121108925812578 1102 44-45 0.7866579717752415 1102 46-47 0.7105338194895907 1102 48-49 0.6343234429213283 1102 50-51 0.5606984201246732 1102 52-53 0.5265711384527947 1102 54-55 0.7107801600331598 1102 56-57 0.7063164259106145 1102 58-59 0.6917506320931892 1102 60-61 0.3679267489517102 1102 62-63 0.29484886083405826 1102 64-65 0.2707616425037642 1102 66-67 0.29523984954403915 1102 68-69 0.4292897857148503 1102 70-71 0.39288296402499157 1102 72-73 0.42488720615908093 1102 74-75 0.34332119372576386 1102 76-77 0.3911714764294345 1102 78-79 0.5238923236585613 1102 80-81 0.5884740836225362 1102 82-83 0.6549890623989718 1102 84-85 0.5718445122072922 1102 86-87 0.6017802833863719 1102 88-89 0.6429833049056342 1102 90-91 0.7166857913230551 1102 92-93 0.7360662334490513 1102 94-95 0.6665339888471067 1102 96-97 0.7667577341019189 1102 98-99 0.9372877060025893 1102 100-101 1.123998668087772 1103 1 0.3285268086633977 1103 2 0.39996925150887463 1103 3 0.3345248781006944 1103 4 0.25660162814639875 1103 5 0.23075815248100895 1103 6 0.2976702078400635 1103 7 0.3013315817961768 1103 8 0.3419093404089395 1103 9 0.4778750311710951 1103 10-11 0.45990205784793403 1103 12-13 0.42978546226855485 1103 14-15 0.5213066445317054 1103 16-17 0.5505888901946676 1103 18-19 0.4660714547611917 1103 20-21 0.5662348508338013 1103 22-23 0.44385267197760214 1103 24-25 0.5306570917941542 1103 26-27 0.4333454063681046 1103 28-29 0.5398134198934841 1103 30-31 0.38140786757311673 1103 32-33 0.5626661745393235 1103 34-35 0.6335201384618259 1103 36-37 0.6446811105302501 1103 38-39 0.631185622018613 1103 40-41 0.7175592111118405 1103 42-43 0.6483686507862174 1103 44-45 0.5835258558381682 1103 46-47 0.7286651223762846 1103 48-49 0.7314156981554447 1103 50-51 0.6803072115741742 1103 52-53 0.8835525729642413 1103 54-55 0.7910415040417575 1103 56-57 0.8784523930801384 1103 58-59 0.9468859812326684 1103 60-61 0.8879267405697462 1103 62-63 0.7048223905958935 1103 64-65 0.67401144119696 1103 66-67 0.797257527662147 1103 68-69 0.6206078744932455 1103 70-71 0.8119182336475816 1103 72-73 0.8136885966708114 1103 74-75 0.7413532904960789 1103 76-77 0.8279366227757095 1103 78-79 0.7957553461831317 1103 80-81 0.8117901752586256 1103 82-83 0.8562374719215562 1103 84-85 0.7323533699860612 1103 86-87 0.8672922927831088 1103 88-89 0.8100257628994285 1103 90-91 0.9569826916173731 1103 92-93 0.7163536034517968 1103 94-95 0.7064727837555687 1103 96-97 1.0108899411229864 1103 98-99 0.9930297136614676 1103 100-101 1.287689668150918 1104 1 0.3679736995753089 1104 2 0.4208694526414263 1104 3 0.4183224191385726 1104 4 0.18028197392505518 1104 5 0.30348661919536823 1104 6 0.36516358392536574 1104 7 0.33291525369720887 1104 8 0.3752286353487193 1104 9 0.6407620050976703 1104 10-11 0.45081473071827105 1104 12-13 0.508266504467322 1104 14-15 0.6499052944579446 1104 16-17 0.5573252838977965 1104 18-19 0.625908334417062 1104 20-21 0.5206672836476045 1104 22-23 0.5808183220547178 1104 24-25 0.5750289080547404 1104 26-27 0.7192275674413011 1104 28-29 0.646733606198886 1104 30-31 0.7686736425847016 1104 32-33 0.740400649179584 1104 34-35 0.588342652282229 1104 36-37 0.7178619355319071 1104 38-39 0.7394993812001047 1104 40-41 0.6578240374464244 1104 42-43 0.7815670002618944 1104 44-45 0.7973190116871294 1104 46-47 0.5726538858724126 1104 48-49 0.6603410392287117 1104 50-51 0.5892933754347922 1104 52-53 0.6466048869654699 1104 54-55 0.5806292311570971 1104 56-57 0.7101299286473903 1104 58-59 0.7019446874065594 1104 60-61 0.6276246672654509 1104 62-63 0.5925791927945667 1104 64-65 0.5949545715922895 1104 66-67 0.42703198350253757 1104 68-69 0.504325608349184 1104 70-71 0.599602094090038 1104 72-73 0.6036904202147149 1104 74-75 0.6343784250095794 1104 76-77 0.5292318545340287 1104 78-79 0.7680495543688899 1104 80-81 0.7023627517633457 1104 82-83 0.6785721942598606 1104 84-85 0.6983955633186198 1104 86-87 0.6787822204166396 1104 88-89 0.8557101508670755 1104 90-91 0.751315703297827 1104 92-93 0.6927136090092638 1104 94-95 0.8212287816411212 1104 96-97 0.7677070646344362 1104 98-99 0.8835799029084193 1104 100-101 1.1174305024005449 1105 1 0.29264206206268284 1105 2 0.3620462349289397 1105 3 0.3448924199625516 1105 4 0.27140880094842856 1105 5 0.3284083479998827 1105 6 0.3074033230929203 1105 7 0.29860548548910515 1105 8 0.36050355621645025 1105 9 0.4135096664998059 1105 10-11 0.39043986620421123 1105 12-13 0.4048472514356263 1105 14-15 0.5279223817812309 1105 16-17 0.5189755112068255 1105 18-19 0.5910716580596116 1105 20-21 0.49413323577692125 1105 22-23 0.5099382801299228 1105 24-25 0.49174807786739905 1105 26-27 0.39998786245157447 1105 28-29 0.5562251593744136 1105 30-31 0.37637711258783213 1105 32-33 0.5631404019917667 1105 34-35 0.6730874326790897 1105 36-37 0.6014339793420547 1105 38-39 0.613064210574592 1105 40-41 0.5274423877066141 1105 42-43 0.5290089622068663 1105 44-45 0.5939046468793734 1105 46-47 0.5995305068352792 1105 48-49 0.5359099079453173 1105 50-51 0.5136216155655404 1105 52-53 0.5165317649641494 1105 54-55 0.6090545845332898 1105 56-57 0.6540313327731582 1105 58-59 0.7761284533631709 1105 60-61 0.5058005887486559 1105 62-63 0.38213737683395266 1105 64-65 0.411057401277823 1105 66-67 0.4830314901760815 1105 68-69 0.35854353623502533 1105 70-71 0.38280730914936356 1105 72-73 0.38227321679280024 1105 74-75 0.541908661125369 1105 76-77 0.5791492978630863 1105 78-79 0.6128078416306018 1105 80-81 0.475549426014485 1105 82-83 0.5454061114864643 1105 84-85 0.6374207322405354 1105 86-87 0.6690990329660984 1105 88-89 0.7672753575223723 1105 90-91 0.705932451969197 1105 92-93 0.7294111222677451 1105 94-95 0.776714150484775 1105 96-97 0.7280820483734765 1105 98-99 0.619549640430666 1105 100-101 0.7036088968746554 1106 1 0.22497232104029052 1106 2 0.17218204974582108 1106 3 0.21799962486014834 1106 4 0.2101889477182084 1106 5 0.14801681351688956 1106 6 0.19057099634329688 1106 7 0.17990809150252574 1106 8 0.2284400870997274 1106 9 0.33707425350453946 1106 10-11 0.21802662397802663 1106 12-13 0.1771428122796621 1106 14-15 0.3162008473322899 1106 16-17 0.2584828548026863 1106 18-19 0.26221590031433806 1106 20-21 0.2273330956120745 1106 22-23 0.25778310150747075 1106 24-25 0.23485035394868703 1106 26-27 0.3229738635132904 1106 28-29 0.39099241946323815 1106 30-31 0.35242200462396767 1106 32-33 0.4781340995494432 1106 34-35 0.4155959330539929 1106 36-37 0.35627872817538986 1106 38-39 0.2628676209907326 1106 40-41 0.3221013395448509 1106 42-43 0.3533182011709215 1106 44-45 0.2612073194134794 1106 46-47 0.15704104604307645 1106 48-49 0.2711572431239446 1106 50-51 0.1805228724137038 1106 52-53 0.16181098293227336 1106 54-55 0.2965545354284558 1106 56-57 0.32815473283930885 1106 58-59 0.3008524776238346 1106 60-61 0.37665028054049543 1106 62-63 0.14937716898560893 1106 64-65 0.2877711928758444 1106 66-67 0.1894107455686438 1106 68-69 0.07918074156999211 1106 70-71 0.0696640521341223 1106 72-73 0.2261251993580906 1106 74-75 0.3243976007515599 1106 76-77 0.34044347617676607 1106 78-79 0.36748524117454195 1106 80-81 0.11971160711784634 1106 82-83 0.31783857662006554 1106 84-85 0.40323910300223176 1106 86-87 0.3872882638952895 1106 88-89 0.29746298252692327 1106 90-91 0.3348561577162421 1106 92-93 0.412560842789194 1106 94-95 0.5534866321856953 1106 96-97 0.5670551051378787 1106 98-99 0.6015167724016841 1106 100-101 0.9064802990286154 1107 1 0.1119253734634853 1107 2 0.16635933029793648 1107 3 0.11571515323001336 1107 4 0.07503048628894504 1107 5 0.07436974753569103 1107 6 0.08648198868601753 1107 7 0.2097295411061637 1107 8 0.16058766047262196 1107 9 0.14473653109654805 1107 10-11 0.16920626091800983 1107 12-13 0.1677236958198094 1107 14-15 0.13165398594577482 1107 16-17 0.09816540934241402 1107 18-19 0.134742024551592 1107 20-21 0.12393341525713453 1107 22-23 0.20817688582271643 1107 24-25 0.13310076910507007 1107 26-27 0.22138612921025924 1107 28-29 0.1120547128112932 1107 30-31 0.19587567675923623 1107 32-33 0.12493975488032305 1107 34-35 -0.07192480526527589 1107 36-37 0.08882733988775016 1107 38-39 0.12271636288553367 1107 40-41 0.034898725288115884 1107 42-43 0.04670639208747929 1107 44-45 0.06844215557783428 1107 46-47 5.253332805921218E-4 1107 48-49 0.1307386391258092 1107 50-51 0.1295608172004421 1107 52-53 -0.03614860825275912 1107 54-55 -0.1554066641219478 1107 56-57 0.1319493675133856 1107 58-59 0.022447512751256227 1107 60-61 0.024149163186770295 1107 62-63 -0.16011187470780186 1107 64-65 -0.1050293440779555 1107 66-67 -0.1262145786199298 1107 68-69 -0.08534130793365335 1107 70-71 -0.0575886928900502 1107 72-73 -0.017417744075334696 1107 74-75 0.1078242223964736 1107 76-77 0.13662684542921966 1107 78-79 0.21442396494987648 1107 80-81 0.07593610590766886 1107 82-83 0.019165439051786137 1107 84-85 -0.024459335510552904 1107 86-87 0.01864752763522759 1107 88-89 0.15853106349431556 1107 90-91 0.1810199489033053 1107 92-93 -0.044342648268468565 1107 94-95 0.10141096725297416 1107 96-97 0.1943507503053361 1107 98-99 0.17082663389662045 1107 100-101 0.39000800901116506 1108 1 0.019068793322507105 1108 2 0.13870472793156452 1108 3 0.0426936493381902 1108 4 0.03452300099394279 1108 5 0.06270363857088768 1108 6 0.15025422168527314 1108 7 0.09135517573578511 1108 8 0.15896564505509048 1108 9 0.026419105916346552 1108 10-11 0.20452770389163533 1108 12-13 0.13592610961587326 1108 14-15 0.2213484355252362 1108 16-17 0.22068587856077926 1108 18-19 0.176722739767186 1108 20-21 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-0.8442666648707551 2316 92-93 -0.8680597558190115 2316 94-95 -1.0148787442602547 2316 96-97 -0.9701098146451237 2316 98-99 -1.192557685397066 2316 100-101 -1.7519877784224676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 31.0 5 52.0 6 128.0 7 408.0 8 893.0 9 1736.0 10 3193.0 11 5381.0 12 7570.0 13 9958.0 14 12546.0 15 16428.0 16 20114.0 17 23780.0 18 20023.0 19 14457.0 20 13363.0 21 13742.0 22 15253.0 23 17802.0 24 21779.0 25 27661.0 26 35854.0 27 45511.0 28 58102.0 29 73068.0 30 88834.0 31 107404.0 32 128831.0 33 157100.0 34 195340.0 35 244453.0 36 321490.0 37 437902.0 38 406650.0 39 73059.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.205258063137066 22.171069506959245 15.234534838981551 22.38913759092214 2 15.104319549642774 21.983161226466123 40.8691240929129 22.043395130978205 3 18.367572061259082 27.065306965295093 30.841917348406746 23.725203625039075 4 12.160680865381122 18.423472421090462 39.60019040372855 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27.93198640796895 31.12149523792257 20.400604217499215 26-27 20.487333806003548 28.10081044174944 30.988074778590903 20.42378097365611 28-29 20.413591044955 27.962282212775836 31.13749942269472 20.486627319574445 30-31 20.80877814502674 27.817002884594466 30.960798601714114 20.413420368664685 32-33 20.662356755716697 28.065915371867256 30.77775007835797 20.49397779405808 34-35 20.711887446916982 27.894302163365047 31.135675510703066 20.258134879014904 36-37 20.77759685608481 27.83942092844127 30.9659691639204 20.417013051553525 38-39 20.69028956695209 28.114935917168765 30.928236809425595 20.26653770645355 40-41 20.704924340161625 27.937172027463813 31.025285852350713 20.33261778002385 42-43 20.645336393275915 28.11614268585544 30.5444376878347 20.694083233033943 44-45 20.623441287039086 28.30070203277069 30.47570006216243 20.600156618027793 46-47 20.75381307860385 28.188972801133577 30.184779139952084 20.87243498031048 48-49 20.91587297739739 28.4510810594671 29.82527797813126 20.80776798500425 50-51 21.017009688956836 28.567738377897996 29.45112864473532 20.964123288409848 52-53 20.922350186303458 28.817864344487337 29.300694277000815 20.959091192208383 54-55 20.91174410237127 28.778309476862816 29.398698599152123 20.91124782161379 56-57 20.910644479994627 28.835631053248544 29.25528605362835 20.998438413128476 58-59 20.783519747949445 28.793236964131548 29.58398716994573 20.83925611797328 60-61 20.970318400115566 29.002300191192166 29.390474174153404 20.636907234538867 62-63 20.885835741414027 28.78557745539105 29.46936014621032 20.85922665698461 64-65 20.64597020453249 28.620965794937973 29.530624012145612 21.202439988383922 66-67 20.635838692192763 28.62617217775718 29.456982728882814 21.28100640116724 68-69 20.697952982712167 28.765723016255496 29.324000458391673 21.21232354264066 70-71 20.821105786603397 28.446427420260388 29.508959067137575 21.223507725998637 72-73 20.8702596763856 28.79038083537849 29.24099961903655 21.098359869199363 74-75 20.83193966813802 28.944774119082407 29.13346426770006 21.089821945079514 76-77 20.652649305700976 28.83943219915828 29.247725272117293 21.260193223023446 78-79 20.59186884122046 28.840212742526923 29.2357029857204 21.33221543053222 80-81 20.659612287795145 28.93121694333405 29.10944669195739 21.299724076913417 82-83 20.838845558704914 28.834085133442294 29.34801029338907 20.979059014463722 84-85 20.88023973122987 28.870231082157034 28.967161004819204 21.282368181793892 86-87 20.569640303916053 28.970825219261247 29.160963596792893 21.298570880029803 88-89 20.830735460068325 28.802107994044885 29.005815610064616 21.361340935822177 90-91 20.608590532603554 28.67505461313517 29.341348222052826 21.375006632208446 92-93 20.758872714119512 28.87806824070831 29.080360491739288 21.282698553432887 94-95 20.409554907049724 28.734113945031975 29.15598626419608 21.700344883722217 96-97 20.471229607780046 28.685733566469068 29.165116054494522 21.677920771256364 98-99 20.45208009056717 28.850230578327547 28.986913410795843 21.710775920309445 100-101 20.38906323594411 28.72631524305574 28.997142676113214 21.88747884488693 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 567.0 1 393.0 2 301.5 3 425.0 4 379.5 5 352.5 6 626.5 7 1456.0 8 2442.0 9 2852.0 10 2578.5 11 2200.5 12 2254.5 13 2535.5 14 2868.5 15 3217.5 16 3608.0 17 4015.0 18 4504.5 19 4946.0 20 5505.5 21 6385.0 22 7461.0 23 9052.5 24 11129.0 25 13442.0 26 17220.5 27 21574.5 28 25922.5 29 32679.5 30 40052.5 31 47108.0 32 56616.0 33 66765.5 34 75729.0 35 83098.0 36 92064.5 37 102045.5 38 104953.5 39 104049.5 40 102176.0 41 99358.5 42 97634.0 43 99586.0 44 102838.5 45 105199.0 46 104122.5 47 101206.0 48 101683.0 49 100819.5 50 96874.5 51 90130.5 52 81423.0 53 73105.0 54 65637.5 55 56665.0 56 46217.5 57 36378.5 58 28458.5 59 21903.0 60 15708.0 61 10420.5 62 6810.5 63 4391.5 64 2649.5 65 1534.0 66 847.0 67 465.0 68 254.0 69 129.5 70 76.5 71 46.0 72 31.0 73 19.5 74 11.5 75 11.0 76 9.0 77 2.5 78 2.5 79 2.5 80 0.5 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004198579048174496 2 0.005610828364378645 3 0.0017176005197077483 4 0.1694317490440599 5 0.0018321072210215983 6 2.290134026276998E-4 7 3.816890043794996E-5 8 4.961957056933495E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.0077345459847463E-4 24-25 6.297868572261744E-4 26-27 0.002538231879123673 28-29 1.145067013138499E-4 30-31 0.005916179567882244 32-33 0.02080205073868273 34-35 0.012767497196494263 36-37 6.107024070071994E-4 38-39 1.7176005197077482E-4 40-41 0.001908445021897498 42-43 0.01057278542131214 44-45 0.007042162130801768 46-47 0.010152927516494692 48-49 0.009866660763210066 50-51 0.18683676764376508 52-53 0.11324712759939753 54-55 0.017137836296639532 56-57 0.0062215307713858435 58-59 0.01753860975123801 60-61 0.12194963689925013 62-63 0.2353112711999615 64-65 0.24103660626565404 66-67 0.2512467871328056 68-69 0.24613215447412035 70-71 0.2339562752344143 72-73 0.16021395958829499 74-75 0.2641287910306137 76-77 0.20574945781076928 78-79 0.005629912814597619 80-81 3.816890043794996E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.542225109487491E-5 90-91 0.0054581527626268445 92-93 0.01742410304992416 94-95 0.0025000629786857227 96-97 3.816890043794996E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2619934.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.92772145222636 #Duplication Level Percentage of deduplicated Percentage of total 1 70.39680502874397 33.03561657515585 2 15.999422819317813 15.01632914922681 3 5.586006628223915 7.864156892387459 4 2.601124700058386 4.88259421547383 5 1.4375961182072603 3.373155509801609 6 0.9122373702733375 2.568553272629919 7 0.5605610768845248 1.841409785109792 8 0.41329886016526807 1.5516139029086682 9 0.2941217815233267 1.2422218532723326 >10 1.556191232805142 13.550181859266141 >50 0.15018030513826386 4.919189676073634 >100 0.08739392401177686 7.393376341079466 >500 0.0039175390815979145 1.1748600588271148 >1k 8.977693728640123E-4 0.6355925892810981 >5k 1.6323079506618405E-4 0.5471956205519161 >10k+ 8.161539753309203E-5 0.4039526989543682 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10547 0.40256739291905824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8711 0.33248929171498215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5576 0.212829788842009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.145067013138499E-4 0.0 10-11 0.0 0.0 0.0 1.145067013138499E-4 0.0 12-13 0.0 0.0 0.0 1.145067013138499E-4 0.0 14-15 0.0 0.0 0.0 1.145067013138499E-4 0.0 16-17 0.0 0.0 0.0 1.145067013138499E-4 0.0 18-19 0.0 0.0 0.0 1.145067013138499E-4 0.0 20-21 0.0 3.816890043794996E-5 0.0 1.3359115153282488E-4 0.0 22-23 0.0 3.816890043794996E-5 0.0 3.4352010394154964E-4 0.0 24-25 0.0 3.816890043794996E-5 0.0 4.389423550364246E-4 0.0 26-27 0.0 3.816890043794996E-5 0.0 5.152801559123245E-4 0.0 28-29 0.0 3.816890043794996E-5 0.0 0.001240489264233374 0.0 30-31 0.0 3.816890043794996E-5 0.0 0.003397032138977547 0.0 32-33 0.0 3.816890043794996E-5 0.0 0.009179620555326966 0.0 34-35 0.0 3.816890043794996E-5 0.0 0.017519525301019034 0.0 36-37 0.0 3.816890043794996E-5 0.0 0.031584765112403594 0.0 38-39 0.0 3.816890043794996E-5 0.0 0.05622279034510029 0.0 40-41 0.0 3.816890043794996E-5 0.0 0.0834563008075776 0.0 42-43 0.0 3.816890043794996E-5 0.0 0.11122417587618619 0.0 44-45 0.0 3.816890043794996E-5 0.0 0.14500365276377192 0.0 46-47 0.0 3.816890043794996E-5 0.0 0.18855436816347282 0.0 48-49 0.0 3.816890043794996E-5 0.0 0.2336509240309107 0.0 50-51 0.0 3.816890043794996E-5 0.0 0.28370943695528206 0.0 52-53 0.0 3.816890043794996E-5 0.0 0.33571456380198894 0.0 54-55 0.0 3.816890043794996E-5 0.0 0.3921472830994979 0.0 56-57 0.0 3.816890043794996E-5 0.0 0.45966806797423143 0.0 58-59 0.0 3.816890043794996E-5 0.0 0.5383723406772842 0.0 60-61 0.0 3.816890043794996E-5 0.0 0.626198980585007 0.0 62-63 0.0 3.816890043794996E-5 0.0 0.7155905454106859 0.0 64-65 0.0 3.816890043794996E-5 0.0 0.808627240228189 0.0 66-67 0.0 3.816890043794996E-5 0.0 0.9198705005545941 0.0 68-69 0.0 3.816890043794996E-5 0.0 1.0492249041388066 0.0 70-71 0.0 5.725335065692494E-5 0.0 1.1910796226164475 0.0 72-73 0.0 7.633780087589992E-5 0.0 1.341026147986934 0.0 74-75 0.0 7.633780087589992E-5 0.0 1.495190336855814 0.0 76-77 0.0 7.633780087589992E-5 0.0 1.6693931984546175 0.0 78-79 0.0 7.633780087589992E-5 0.0 1.8763258921789632 0.0 80-81 0.0 7.633780087589992E-5 0.0 2.098621568329584 0.0 82-83 0.0 7.633780087589992E-5 0.0 2.336585578109983 0.0 84-85 0.0 7.633780087589992E-5 0.0 2.5687288305735945 0.0 86-87 0.0 7.633780087589992E-5 0.0 2.8202046311090276 0.0 88-89 0.0 7.633780087589992E-5 0.0 3.115059386992191 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4910 0.0 58.66247 1 GTATCAA 9440 0.0 43.149727 1 GTGGTAT 2105 0.0 33.417877 1 ATCAACG 12085 0.0 33.11322 3 TATCAAC 12440 0.0 32.24653 2 CAACGCA 12415 0.0 32.137623 5 TCAACGC 12490 0.0 31.961557 4 AACGCAG 12930 0.0 31.15052 6 ACGCAGA 14615 0.0 27.104136 7 CGCAGAG 14645 0.0 27.04861 8 TGGTATC 2420 0.0 25.53224 2 GCAGAGT 16255 0.0 23.552362 9 GTACATG 9525 0.0 23.3534 1 TACATGG 9930 0.0 22.161167 2 ACATGGG 9510 0.0 22.089087 3 GAGTACT 10600 0.0 21.885218 12-13 AGAGTAC 15170 0.0 21.171469 10-11 CAGAGTA 15765 0.0 20.522879 10-11 AGTACTT 11705 0.0 19.738106 12-13 GTACTTT 11685 0.0 19.731289 14-15 >>END_MODULE