FastQCFastQC Report
Sat 18 Jun 2016
SRR1930984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660373
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310974.709005365149696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5300.056.4374771
GTATCAA9900.049.4884531
TCAACGC12350.039.3969884
ATCAACG12400.038.8385123
CAACGCA12450.038.6643875
TATCAAC12550.038.6185462
GTACTAC859.277184E-636.4038931
AACGCAG13900.034.2718776
ACGCAGA15850.029.1263647
GTGGTAT2002.746674E-1028.3646981
CGCAGAG16550.027.4236228
GCAGAGT17300.025.8030199
ATACCGT950.001352313224.5809636
CAGAGTA16750.021.3704410-11
CTACGAC1200.003264213221.1227023
TGGTATC2851.6600097E-819.90032
GAGTACT13550.019.8558912-13
GTATATG1858.080281E-519.5137981
AGAGTAC15650.018.7933410-11
GTATAGG1400.007208594618.4186341
Produced by FastQC (version 0.11.5)