##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930984_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 660373 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.93142966172148 28.0 2.0 31.0 2.0 33.0 2 19.57413158926849 27.0 2.0 31.0 2.0 33.0 3 20.246306254192707 27.0 2.0 31.0 2.0 33.0 4 21.66986233537713 30.0 2.0 35.0 2.0 37.0 5 20.956273197117387 30.0 2.0 35.0 2.0 37.0 6 22.020084103983656 30.0 2.0 35.0 2.0 37.0 7 22.256153719185974 30.0 2.0 35.0 2.0 37.0 8 22.740947918827693 31.0 2.0 35.0 2.0 37.0 9 24.092387181183966 32.0 2.0 37.0 2.0 39.0 10-11 24.506151069168485 32.0 2.0 37.0 2.0 39.0 12-13 24.452610872946046 32.0 2.0 37.0 2.0 39.0 14-15 24.40761206166818 32.0 2.0 38.0 2.0 40.0 16-17 23.154267361021724 31.5 2.0 38.0 2.0 40.0 18-19 22.045525786184474 28.5 2.0 38.0 2.0 40.0 20-21 21.69817285079796 27.5 2.0 37.5 2.0 40.0 22-23 21.316276558853858 27.0 2.0 37.0 2.0 40.0 24-25 21.164291998612903 27.0 2.0 37.0 2.0 39.5 26-27 20.90042748567855 26.5 2.0 37.0 2.0 39.0 28-29 20.711582696445795 25.5 2.0 37.0 2.0 39.0 30-31 20.70929156703863 25.5 2.0 37.0 2.0 39.0 32-33 20.571009111517277 25.5 2.0 36.5 2.0 39.0 34-35 20.23671167658278 25.0 2.0 36.0 2.0 39.0 36-37 19.7644073879459 24.0 2.0 36.0 2.0 39.0 38-39 19.669884292664904 24.0 2.0 35.5 2.0 39.0 40-41 19.545906631555198 24.0 2.0 35.0 2.0 39.0 42-43 19.40527171765048 23.5 2.0 35.0 2.0 39.0 44-45 19.023395868698447 22.0 2.0 35.0 2.0 38.5 46-47 18.688613859137185 19.0 2.0 35.0 2.0 38.0 48-49 18.462103992743494 18.5 2.0 35.0 2.0 38.0 50-51 16.200130078001372 10.0 2.0 32.5 2.0 37.5 52-53 18.09300349953738 18.5 2.0 34.0 2.0 38.0 54-55 18.859568758868093 22.0 2.0 35.5 2.0 39.0 56-57 18.848811202153932 21.5 2.0 36.0 2.0 39.0 58-59 18.575746585641753 19.5 2.0 35.0 2.0 39.0 60-61 18.305053356209292 18.0 2.0 35.0 2.0 39.0 62-63 17.862676850810075 14.5 2.0 34.0 2.0 38.5 64-65 17.530765945912385 13.0 2.0 34.0 2.0 38.0 66-67 17.190676329892348 8.0 2.0 34.0 2.0 38.0 68-69 16.76525009350776 7.5 2.0 33.5 2.0 37.5 70-71 16.43028636846146 7.0 2.0 33.0 2.0 37.0 72-73 16.031286106488302 2.0 2.0 32.0 2.0 36.0 74-75 15.585321477407465 2.0 2.0 32.0 2.0 36.0 76-77 15.200069506173026 2.0 2.0 31.0 2.0 35.0 78-79 14.856943727257171 2.0 2.0 31.0 2.0 35.0 80-81 14.4377707750014 2.0 2.0 31.0 2.0 35.0 82-83 14.002014013292488 2.0 2.0 30.0 2.0 34.0 84-85 13.629335239326865 2.0 2.0 30.0 2.0 34.0 86-87 13.158674718681716 2.0 2.0 29.0 2.0 34.0 88-89 12.750674997312125 2.0 2.0 29.0 2.0 34.0 90-91 12.28346404229125 2.0 2.0 29.0 2.0 34.0 92-93 11.746459198059277 2.0 2.0 28.0 2.0 34.0 94-95 11.189872238871063 2.0 2.0 26.5 2.0 34.0 96-97 10.547705614857058 2.0 2.0 25.0 2.0 33.0 98-99 9.866907793020005 2.0 2.0 24.0 2.0 33.0 100-101 8.927306234506862 2.0 2.0 10.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.121570914283769 1101 2 9.870471952228566 1101 3 9.063238816230847 1101 4 11.611961231550424 1101 5 12.122910918554744 1101 6 11.237457160899705 1101 7 10.888478788142557 1101 8 10.298961266518713 1101 9 10.248452598268646 1101 10-11 9.499589718306044 1101 12-13 9.372851046738933 1101 14-15 10.111590248252046 1101 16-17 11.114981405581158 1101 18-19 12.142094145532806 1101 20-21 12.356575886128102 1101 22-23 12.386041486531173 1101 24-25 12.552435918235446 1101 26-27 12.585336484725744 1101 28-29 12.25888615378765 1101 30-31 11.831428312550482 1101 32-33 11.659731576518961 1101 34-35 11.675826739441224 1101 36-37 12.280679478337465 1101 38-39 12.280966884197252 1101 40-41 11.822454741027464 1101 42-43 11.305725340670111 1101 44-45 10.978329923078455 1101 46-47 10.737018839713777 1101 48-49 10.88055084920924 1101 50-51 12.757637140635335 1101 52-53 12.074356271176335 1101 54-55 11.908610808375556 1101 56-57 11.770280241805782 1101 58-59 11.698638956370566 1101 60-61 11.393088412238654 1101 62-63 11.39919432913683 1101 64-65 11.221999444019271 1101 66-67 10.839598997616086 1101 68-69 10.569857191394124 1101 70-71 10.532907957825163 1101 72-73 10.541764409694501 1101 74-75 10.293551408527776 1101 76-77 10.047611012568824 1101 78-79 9.617905129257013 1101 80-81 9.654337771110313 1101 82-83 9.498530807007683 1101 84-85 9.2125340819452 1101 86-87 8.962736757626839 1101 88-89 9.032159933772377 1101 90-91 8.512794759404393 1101 92-93 8.263892535491628 1101 94-95 7.965010809917038 1101 96-97 7.987301024165308 1101 98-99 7.540803881308062 1101 100-101 6.784624995878199 1102 1 8.132485749843152 1102 2 9.342274438841482 1102 3 8.327623523151097 1102 4 10.97881357352125 1102 5 11.569070209500765 1102 6 10.632440337280986 1102 7 10.275445099871902 1102 8 9.535174105812121 1102 9 9.336261116764366 1102 10-11 8.78417174370561 1102 12-13 8.7829511164954 1102 14-15 9.455310114483769 1102 16-17 10.199276454923602 1102 18-19 11.605324536784526 1102 20-21 11.607060590996692 1102 22-23 11.86788044141515 1102 24-25 11.152850610178575 1102 26-27 11.468544410291486 1102 28-29 11.27522634992642 1102 30-31 11.449146198929501 1102 32-33 11.365037427855622 1102 34-35 11.172692827539539 1102 36-37 11.22188657298058 1102 38-39 11.361522781696276 1102 40-41 10.839389918803874 1102 42-43 10.796917304314679 1102 44-45 10.544215163430522 1102 46-47 10.193932680217667 1102 48-49 9.760764632266625 1102 50-51 11.873825257415866 1102 52-53 10.815344351026976 1102 54-55 10.512282511869532 1102 56-57 10.285807467344856 1102 58-59 10.421055658941295 1102 60-61 10.479830067108274 1102 62-63 10.496612313995573 1102 64-65 10.506381432488936 1102 66-67 10.450975969775051 1102 68-69 10.128341065340084 1102 70-71 9.740374464462285 1102 72-73 9.305965749429017 1102 74-75 8.831917465851184 1102 76-77 8.97480013466316 1102 78-79 8.893438048154865 1102 80-81 8.343395751045072 1102 82-83 8.69221528209026 1102 84-85 7.8828014133499735 1102 86-87 7.735670478724067 1102 88-89 7.765297846412658 1102 90-91 7.349565906898292 1102 92-93 7.217516024956346 1102 94-95 7.059216663305358 1102 96-97 7.362000968770467 1102 98-99 6.553817822343326 1102 100-101 5.49511385535995 1103 1 7.917809158797873 1103 2 9.134746470813432 1103 3 8.812623052684046 1103 4 10.804639243193954 1103 5 11.121455477444137 1103 6 10.544163385709378 1103 7 10.444065442867142 1103 8 9.840497316675457 1103 9 9.45081489114818 1103 10-11 8.593549637696999 1103 12-13 8.508067123765347 1103 14-15 9.390139285421771 1103 16-17 10.274800035227369 1103 18-19 11.577410983866887 1103 20-21 11.644585290516819 1103 22-23 11.9643274247309 1103 24-25 11.813995479363975 1103 26-27 11.579486731031153 1103 28-29 11.287519158713756 1103 30-31 10.845059078336416 1103 32-33 10.72861312600396 1103 34-35 11.30715231042197 1103 36-37 11.658814274575711 1103 38-39 11.32188717080719 1103 40-41 11.173859801484248 1103 42-43 10.493337297678618 1103 44-45 10.036463599746615 1103 46-47 9.98452779627446 1103 48-49 9.949180495425093 1103 50-51 11.521239917169147 1103 52-53 10.795359405972572 1103 54-55 10.117326661487962 1103 56-57 9.944513336297497 1103 58-59 9.830888172671507 1103 60-61 10.033090651378828 1103 62-63 9.803135215836338 1103 64-65 9.214190442675786 1103 66-67 9.319502714588769 1103 68-69 8.853136412173345 1103 70-71 9.008445351466008 1103 72-73 8.556402788557019 1103 74-75 8.537953558102341 1103 76-77 8.347263945842444 1103 78-79 7.707022908029964 1103 80-81 7.560461819475744 1103 82-83 7.387637390079782 1103 84-85 7.471490642625902 1103 86-87 7.2728856604481535 1103 88-89 7.121333691049578 1103 90-91 6.3879906841692815 1103 92-93 6.136625406069324 1103 94-95 6.210392583316052 1103 96-97 5.740995605446095 1103 98-99 5.7941905808154495 1103 100-101 5.196598797938208 1104 1 7.819874128520215 1104 2 9.336060801922123 1104 3 8.842698544666295 1104 4 10.987249448644405 1104 5 11.637952402109129 1104 6 10.701533486698342 1104 7 10.222660350131399 1104 8 9.693808831700121 1104 9 9.577298448927845 1104 10-11 8.651382822773659 1104 12-13 8.557222536402524 1104 14-15 9.419785467297036 1104 16-17 10.556419140835285 1104 18-19 11.031870814235877 1104 20-21 11.0701743134974 1104 22-23 11.633847834204747 1104 24-25 11.56865367240843 1104 26-27 11.792918072333876 1104 28-29 11.5195095402646 1104 30-31 11.262622023998365 1104 32-33 11.447129592008782 1104 34-35 11.178433010256903 1104 36-37 11.07401304158757 1104 38-39 10.965172094768167 1104 40-41 10.183604308455486 1104 42-43 10.105935468175382 1104 44-45 10.647376638881624 1104 46-47 10.545796651431651 1104 48-49 10.738137117265918 1104 50-51 12.148427495955282 1104 52-53 11.416403450097565 1104 54-55 10.548692937930372 1104 56-57 10.860803811559247 1104 58-59 10.909159372979751 1104 60-61 10.251297918968646 1104 62-63 10.084200142746592 1104 64-65 9.948152558734602 1104 66-67 9.683486331829673 1104 68-69 9.462151448730108 1104 70-71 9.167160465560222 1104 72-73 9.050806297245494 1104 74-75 8.732744991879176 1104 76-77 8.824247691519615 1104 78-79 8.570750841966158 1104 80-81 8.30233109038588 1104 82-83 8.305314501310342 1104 84-85 7.330806553024956 1104 86-87 7.389183508902878 1104 88-89 7.120509558351134 1104 90-91 7.167893738573934 1104 92-93 6.561730414607958 1104 94-95 6.273695012664215 1104 96-97 5.903658945454943 1104 98-99 5.400950847392053 1104 100-101 4.987141845492925 1105 1 7.648763072199706 1105 2 8.90424633884638 1105 3 8.467330108985358 1105 4 10.400845537790708 1105 5 10.26406555417024 1105 6 9.95737074067296 1105 7 9.554473244203479 1105 8 9.394268101485817 1105 9 9.290988902437146 1105 10-11 8.5771868811137 1105 12-13 7.8479308967264565 1105 14-15 9.230501986440007 1105 16-17 10.06518426771164 1105 18-19 10.454781181539634 1105 20-21 10.867738099099771 1105 22-23 11.198296900983781 1105 24-25 10.95467003935805 1105 26-27 11.42301201082445 1105 28-29 11.07037663893027 1105 30-31 10.943552801798308 1105 32-33 10.697178343193944 1105 34-35 10.89604485219996 1105 36-37 10.901483157671425 1105 38-39 10.767238920875492 1105 40-41 10.45408372873105 1105 42-43 10.351984380743776 1105 44-45 10.210802491130785 1105 46-47 9.538418972077721 1105 48-49 8.84324915533443 1105 50-51 10.66517661002857 1105 52-53 10.163049704773758 1105 54-55 9.863478780137914 1105 56-57 9.756469551019467 1105 58-59 9.471962106632414 1105 60-61 9.360666439824072 1105 62-63 9.391947882820961 1105 64-65 9.394155297766705 1105 66-67 9.326834989098753 1105 68-69 9.496717720082568 1105 70-71 8.901611078928582 1105 72-73 8.467049519710041 1105 74-75 8.19922771154529 1105 76-77 7.912113017211153 1105 78-79 7.765064100271227 1105 80-81 7.680760203921228 1105 82-83 7.86057256879095 1105 84-85 7.166312209283152 1105 86-87 6.792156322552316 1105 88-89 6.602118594019068 1105 90-91 6.220957442363975 1105 92-93 6.060422537985438 1105 94-95 5.90849596213557 1105 96-97 5.346827391446478 1105 98-99 4.53941429969322 1105 100-101 4.026749101462423 1106 1 8.032673086447886 1106 2 9.308903358580327 1106 3 8.957221308664664 1106 4 10.640894687475026 1106 5 11.543695885799892 1106 6 10.582460608779261 1106 7 9.831813845415667 1106 8 9.231583953566183 1106 9 8.790195884480955 1106 10-11 8.480997506682865 1106 12-13 8.548609101368665 1106 14-15 9.02727043826852 1106 16-17 10.260716570121627 1106 18-19 11.356387042900923 1106 20-21 11.474268296962105 1106 22-23 11.610208727844427 1106 24-25 11.044139458058453 1106 26-27 11.562247663517446 1106 28-29 11.60868550635248 1106 30-31 11.176774154024638 1106 32-33 11.434582647277114 1106 34-35 10.897716943632314 1106 36-37 11.159702004483886 1106 38-39 10.881304967942416 1106 40-41 10.80403013466038 1106 42-43 10.014975714190395 1106 44-45 10.153345725859744 1106 46-47 9.970624651306316 1106 48-49 9.993631721139671 1106 50-51 11.978478614795392 1106 52-53 11.371755037601648 1106 54-55 10.883467625599632 1106 56-57 10.613667496144366 1106 58-59 10.647837803246404 1106 60-61 10.30405171147629 1106 62-63 10.507017184747298 1106 64-65 10.064994023186419 1106 66-67 9.661271793846403 1106 68-69 9.286563604450091 1106 70-71 8.616131150561628 1106 72-73 8.718221617678257 1106 74-75 8.573155285476787 1106 76-77 8.712539373293643 1106 78-79 7.777810121020414 1106 80-81 7.402972985453495 1106 82-83 7.356099860373195 1106 84-85 7.4554467739729375 1106 86-87 6.983737696216274 1106 88-89 6.951666312349552 1106 90-91 5.945635333284791 1106 92-93 5.585548571196334 1106 94-95 5.119765531596537 1106 96-97 5.007557316545494 1106 98-99 4.856502747338785 1106 100-101 4.3585748292306885 1107 1 7.3733685516746235 1107 2 8.37752492750188 1107 3 8.3762753559623 1107 4 9.908406311893806 1107 5 11.15851514483694 1107 6 9.669431260246686 1107 7 9.681796346290618 1107 8 8.261082478639928 1107 9 5.023096739438209 1107 10-11 7.39115824864577 1107 12-13 7.415522005723442 1107 14-15 8.114653569111983 1107 16-17 9.103599425978839 1107 18-19 10.460363094098366 1107 20-21 10.836031458804012 1107 22-23 10.184967489906324 1107 24-25 10.40519015552358 1107 26-27 10.334671542323505 1107 28-29 10.497178581698716 1107 30-31 10.026669159274377 1107 32-33 10.503341709324008 1107 34-35 10.514186120173488 1107 36-37 11.119341522507984 1107 38-39 10.984668549763324 1107 40-41 10.422549208706677 1107 42-43 9.898950265462833 1107 44-45 9.686612812505413 1107 46-47 9.991242370420359 1107 48-49 9.84782651140016 1107 50-51 11.670800248713697 1107 52-53 10.728118469430058 1107 54-55 10.53667871504516 1107 56-57 10.33780628920471 1107 58-59 10.138000795096808 1107 60-61 9.697632032460241 1107 62-63 10.065008035204773 1107 64-65 10.288489098432656 1107 66-67 10.130779568457676 1107 68-69 9.973385513354646 1107 70-71 9.768536030317641 1107 72-73 9.17063024724678 1107 74-75 9.342885548963615 1107 76-77 8.684515774473587 1107 78-79 8.545990462003362 1107 80-81 8.134811393533091 1107 82-83 7.890223154208503 1107 84-85 7.583727859918639 1107 86-87 7.2365875537234 1107 88-89 7.353239983665983 1107 90-91 7.242539238089549 1107 92-93 6.583161190565363 1107 94-95 6.209998519947122 1107 96-97 5.829484970162813 1107 98-99 5.151088018075249 1107 100-101 5.124649025520874 1108 1 6.512216500581527 1108 2 7.974749196723202 1108 3 7.504867839381607 1108 4 9.259966125207491 1108 5 10.863575326610459 1108 6 8.887141896454008 1108 7 8.459516808093618 1108 8 5.316154942408044 1108 9 6.919989394805441 1108 10-11 5.701370727152277 1108 12-13 4.430942049938626 1108 14-15 6.71052681892775 1108 16-17 8.189708490090847 1108 18-19 8.022540691740222 1108 20-21 8.122748481648525 1108 22-23 9.160022758882008 1108 24-25 8.738124324032547 1108 26-27 7.479132362760744 1108 28-29 9.377122084523577 1108 30-31 9.470528161976414 1108 32-33 10.06381825071433 1108 34-35 9.506534437540228 1108 36-37 8.96733955738885 1108 38-39 8.911989846741953 1108 40-41 8.916297673457354 1108 42-43 8.34489106624888 1108 44-45 7.933376506929388 1108 46-47 8.476043402107905 1108 48-49 8.925817177084266 1108 50-51 10.534660479615194 1108 52-53 9.877584202665137 1108 54-55 9.236930495934374 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-16.334734637914575 2313 54-55 -17.08408399681925 2313 56-57 -17.063323654298113 2313 58-59 -16.78431558908398 2313 60-61 -16.524119379969136 2313 62-63 -16.09106541112291 2313 64-65 -15.77718886766904 2313 66-67 -15.425011391994303 2313 68-69 -14.987772678735748 2313 70-71 -14.653780353863148 2313 72-73 -14.238268057837967 2313 74-75 -13.777877162900793 2313 76-77 -13.398817558859749 2313 78-79 -13.093133831781946 2313 80-81 -12.658973917201372 2313 82-83 -12.171923738446864 2313 84-85 -11.80045293104181 2313 86-87 -11.31567233328225 2313 88-89 -10.904528377915938 2313 90-91 -10.44743251332288 2313 92-93 -9.904126945027855 2313 94-95 -9.350735943329715 2313 96-97 -8.678283391419107 2313 98-99 -7.974618196614017 2313 100-101 -6.995407471654268 2314 1 -19.060247267534415 2314 2 -17.738294281537666 2314 3 -18.38643650844448 2314 4 -19.845830976241785 2314 5 -19.146569600925773 2314 6 -20.231698683256138 2314 7 -20.40859913393537 2314 8 -20.90558418802674 2314 9 -22.246677271861227 2314 10-11 -22.639208982992653 2314 12-13 -22.592246355858475 2314 14-15 -22.56048767382587 2314 16-17 -21.363914698314947 2314 18-19 -20.256444815506153 2314 20-21 -19.90478775023553 2314 22-23 -19.525484487494804 2314 24-25 -19.385064081764554 2314 26-27 -19.17521546332621 2314 28-29 -18.95621124880976 2314 30-31 -18.93237038874822 2314 32-33 -18.842703488416102 2314 34-35 -18.47027715666267 2314 36-37 -18.026138703480715 2314 38-39 -17.8959811677508 2314 40-41 -17.782253051180327 2314 42-43 -17.639891542446772 2314 44-45 -17.24488436263583 2314 46-47 -16.92613051093557 2314 48-49 -16.697371228712836 2314 50-51 -14.416064158065964 2314 52-53 -16.334734637914575 2314 54-55 -17.08408399681925 2314 56-57 -17.063323654298113 2314 58-59 -16.78431558908398 2314 60-61 -16.524119379969136 2314 62-63 -16.09106541112291 2314 64-65 -15.77718886766904 2314 66-67 -15.425011391994303 2314 68-69 -14.987772678735748 2314 70-71 -14.653780353863148 2314 72-73 -14.238268057837967 2314 74-75 -13.777877162900793 2314 76-77 -13.398817558859749 2314 78-79 -13.093133831781946 2314 80-81 -12.658973917201372 2314 82-83 -12.171923738446864 2314 84-85 -11.80045293104181 2314 86-87 -11.31567233328225 2314 88-89 -10.904528377915938 2314 90-91 -10.44743251332288 2314 92-93 -9.904126945027855 2314 94-95 -9.350735943329715 2314 96-97 -8.678283391419107 2314 98-99 -7.974618196614017 2314 100-101 -6.995407471654268 2315 1 -19.060247267534415 2315 2 -17.738294281537666 2315 3 -18.38643650844448 2315 4 -19.845830976241785 2315 5 -19.146569600925773 2315 6 -20.231698683256138 2315 7 -20.40859913393537 2315 8 -20.90558418802674 2315 9 -22.246677271861227 2315 10-11 -22.639208982992653 2315 12-13 -22.592246355858475 2315 14-15 -22.56048767382587 2315 16-17 -21.363914698314947 2315 18-19 -20.256444815506153 2315 20-21 -19.90478775023553 2315 22-23 -19.525484487494804 2315 24-25 -19.385064081764554 2315 26-27 -19.17521546332621 2315 28-29 -18.95621124880976 2315 30-31 -18.93237038874822 2315 32-33 -18.842703488416102 2315 34-35 -18.47027715666267 2315 36-37 -18.026138703480715 2315 38-39 -17.8959811677508 2315 40-41 -17.782253051180327 2315 42-43 -17.639891542446772 2315 44-45 -17.24488436263583 2315 46-47 -16.92613051093557 2315 48-49 -16.697371228712836 2315 50-51 -14.416064158065964 2315 52-53 -16.334734637914575 2315 54-55 -17.08408399681925 2315 56-57 -17.063323654298113 2315 58-59 -16.78431558908398 2315 60-61 -16.524119379969136 2315 62-63 -16.09106541112291 2315 64-65 -15.77718886766904 2315 66-67 -15.425011391994303 2315 68-69 -14.987772678735748 2315 70-71 -14.653780353863148 2315 72-73 -14.238268057837967 2315 74-75 -13.777877162900793 2315 76-77 -13.398817558859749 2315 78-79 -13.093133831781946 2315 80-81 -12.658973917201372 2315 82-83 -12.171923738446864 2315 84-85 -11.80045293104181 2315 86-87 -11.31567233328225 2315 88-89 -10.904528377915938 2315 90-91 -10.44743251332288 2315 92-93 -9.904126945027855 2315 94-95 -9.350735943329715 2315 96-97 -8.678283391419107 2315 98-99 -7.974618196614017 2315 100-101 -6.995407471654268 2316 1 -19.060247267534415 2316 2 -17.738294281537666 2316 3 -18.38643650844448 2316 4 -19.845830976241785 2316 5 -19.146569600925773 2316 6 -20.231698683256138 2316 7 -20.40859913393537 2316 8 -20.90558418802674 2316 9 -22.246677271861227 2316 10-11 -22.639208982992653 2316 12-13 -22.592246355858475 2316 14-15 -22.56048767382587 2316 16-17 -21.363914698314947 2316 18-19 -20.256444815506153 2316 20-21 -19.90478775023553 2316 22-23 -19.525484487494804 2316 24-25 -19.385064081764554 2316 26-27 -19.17521546332621 2316 28-29 -18.95621124880976 2316 30-31 -18.93237038874822 2316 32-33 -18.842703488416102 2316 34-35 -18.47027715666267 2316 36-37 -18.026138703480715 2316 38-39 -17.8959811677508 2316 40-41 -17.782253051180327 2316 42-43 -17.639891542446772 2316 44-45 -17.24488436263583 2316 46-47 -16.92613051093557 2316 48-49 -16.697371228712836 2316 50-51 -14.416064158065964 2316 52-53 -16.334734637914575 2316 54-55 -17.08408399681925 2316 56-57 -17.063323654298113 2316 58-59 -16.78431558908398 2316 60-61 -16.524119379969136 2316 62-63 -16.09106541112291 2316 64-65 -15.77718886766904 2316 66-67 -15.425011391994303 2316 68-69 -14.987772678735748 2316 70-71 -14.653780353863148 2316 72-73 -14.238268057837967 2316 74-75 -13.777877162900793 2316 76-77 -13.398817558859749 2316 78-79 -13.093133831781946 2316 80-81 -12.658973917201372 2316 82-83 -12.171923738446864 2316 84-85 -11.80045293104181 2316 86-87 -11.31567233328225 2316 88-89 -10.904528377915938 2316 90-91 -10.44743251332288 2316 92-93 -9.904126945027855 2316 94-95 -9.350735943329715 2316 96-97 -8.678283391419107 2316 98-99 -7.974618196614017 2316 100-101 -6.995407471654268 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 173668.0 3 6294.0 4 26804.0 5 13791.0 6 7511.0 7 7662.0 8 7672.0 9 6937.0 10 7472.0 11 7918.0 12 8213.0 13 8367.0 14 8612.0 15 8919.0 16 9381.0 17 9560.0 18 9326.0 19 9720.0 20 10139.0 21 10982.0 22 11615.0 23 12712.0 24 13241.0 25 14487.0 26 15494.0 27 16716.0 28 18125.0 29 19465.0 30 21289.0 31 23342.0 32 24883.0 33 26659.0 34 28153.0 35 26249.0 36 19752.0 37 8314.0 38 921.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.585793923664 21.24046986955541 14.883224992185987 23.290511214594602 2 15.509640968367112 21.62861741615478 39.892038673367715 22.96970294211039 3 18.313862584892195 25.398903116656108 31.60655724560405 24.680677052847646 4 12.952394331571462 18.00467978998016 38.38371643809156 30.659209440356822 5 13.062061417181017 37.4148811626731 35.71723805103551 13.805819369110377 6 29.948429403478134 37.810885844447924 17.953322843467586 14.287361908606359 7 25.503775523881867 33.39683416534317 23.186062853638028 17.913327457136937 8 24.23501342081718 35.575459626910906 21.446562058051047 18.74296489422087 9 24.86339744500383 16.561912054634092 22.25296333126596 36.32172716909612 10-11 23.67890958220405 26.908226612189274 29.427670203347837 19.985193602258835 12-13 24.141567807014603 25.01066895962467 29.334882106863564 21.512881126497167 14-15 21.806869655026794 26.91740687768055 27.080879100300866 24.194844366991795 16-17 21.02645193207059 29.568501230764916 28.159981413346742 21.245065423817756 18-19 20.77534664800916 28.274180553788746 30.237353178136793 20.7131196200653 20-21 21.579968413747125 27.611521878125156 30.235038881693637 20.573470826434086 22-23 21.394140549784442 27.36422648011984 29.905040397809913 21.33659257228581 24-25 21.306399198105076 27.657607041693005 30.139693160270447 20.89630059993147 26-27 21.142342581116793 27.801680829269937 29.907985622685658 21.147990966927615 28-29 21.07908474664312 27.880736565981874 30.10788769706257 20.932290990312445 30-31 21.41231553686056 27.69451140317589 29.89582943606792 20.997343623895624 32-33 21.12719488425156 27.87960815185589 29.6869803528654 21.30621661102715 34-35 21.44830844560253 27.850362731603155 29.690404946321447 21.010923876472862 36-37 21.52907361142116 27.567566412846457 29.560565973850363 21.342794001882023 38-39 21.292786254840316 27.716120462956518 29.76182697509011 21.229266307113058 40-41 21.611450492019735 27.70175167022222 29.564035351200296 21.122762486557747 42-43 21.55858310626703 27.803503308680423 29.192474373945764 21.445439211106788 44-45 21.507326177019497 27.963993756637134 29.18212150121721 21.346558565126156 46-47 21.533221401148552 27.973029334074795 28.988760440171536 21.504988824605118 48-49 21.626592786701607 28.035746434358185 28.792982736892643 21.54467804204757 50-51 21.737788392942907 28.22988763778159 28.53283715950557 21.499486809769934 52-53 21.378203574329653 28.401307203769242 28.60759844346581 21.612890778435293 54-55 21.213028843206214 28.48328175521719 28.616255800653512 21.68743360092308 56-57 21.30501613765273 28.457890877150827 28.649098238737825 21.587994746458623 58-59 21.07826254525476 28.41619618117484 28.789612008347788 21.715929265222613 60-61 21.102659661127404 28.51234115347018 28.805290811339194 21.579708374063213 62-63 21.026322764890658 28.353920791013355 28.84312468961399 21.776631754481997 64-65 21.0743419304964 28.30716178207321 28.769404804899818 21.84909148253058 66-67 20.909257572888773 28.392534955454774 28.62735787152662 22.070849600129833 68-69 21.02186013335149 28.33373638187054 28.63886783327203 22.005535651505944 70-71 21.1695556190065 28.12552541856895 28.62793749396857 22.076981468455983 72-73 21.057682087430578 28.305616620292785 28.48680753333009 22.149893758946547 74-75 20.99705875319831 28.573519213754512 28.19656137162534 22.232860661421842 76-77 21.03213348194497 28.438067351083145 28.357311603653162 22.172487563318725 78-79 20.864911584149436 28.57089900585212 28.366235846488973 22.197953563509476 80-81 20.9863477616679 28.53083400013649 28.15577576819186 22.32704247000375 82-83 21.13825210235554 28.373159969347434 28.190446615946346 22.29814131235068 84-85 20.97611400870206 28.454620651492025 28.198977234189933 22.37028810561599 86-87 20.918506979832987 28.374485132479986 28.245394271544 22.461613616143026 88-89 21.159798893994257 28.348449775668648 28.095521488277193 22.396229842059906 90-91 21.06031233277338 28.302045824749445 28.120534497933004 22.517107344544172 92-93 20.999639324596103 28.235297576810144 28.086126934219863 22.678936164373894 94-95 20.96362892627438 28.092208519366284 28.22420850776189 22.719954046597447 96-97 20.941309121573283 28.058601209952055 28.148078900626345 22.85201076784832 98-99 20.802103742058648 28.180920301925244 27.797121842990162 23.21985411302595 100-101 21.191412103412073 28.132051284530146 27.69391697103402 22.982619641023756 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 41828.0 1 38236.5 2 25617.0 3 13008.0 4 8131.0 5 6331.0 6 5757.0 7 5813.0 8 5961.0 9 5945.5 10 5540.0 11 4829.0 12 3941.5 13 3038.0 14 2324.5 15 1826.0 16 1664.5 17 1721.0 18 1969.0 19 2217.5 20 2558.5 21 2997.0 22 3437.0 23 3916.0 24 4407.5 25 4999.5 26 5638.0 27 6371.5 28 7214.5 29 8155.0 30 9116.0 31 10160.0 32 11349.0 33 12458.0 34 13377.0 35 14300.5 36 15311.5 37 16173.0 38 16815.5 39 17398.0 40 17718.0 41 17747.0 42 17908.5 43 18245.0 44 18451.0 45 18711.5 46 18779.5 47 18517.5 48 18291.0 49 17796.0 50 16972.0 51 16063.5 52 15133.0 53 14016.5 54 12652.0 55 11093.5 56 9466.5 57 7882.5 58 6351.5 59 4931.0 60 3789.0 61 2857.0 62 2047.5 63 1400.5 64 909.5 65 601.5 66 406.0 67 266.0 68 164.0 69 99.5 70 56.5 71 32.5 72 27.5 73 19.0 74 11.5 75 5.5 76 4.5 77 4.0 78 1.0 79 2.0 80 2.0 81 0.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 23.452049069238143 2 30.850443612927847 3 24.45663284234819 4 31.012321824181182 5 34.420244316469635 6 26.473674726253194 7 25.4388050389704 8 21.299780578551818 9 12.591520246890772 10-11 16.442904237453682 12-13 16.435938477193947 14-15 20.75228696509397 16-17 24.393789570439736 18-19 28.576728606408803 20-21 30.724378495183785 22-23 30.400470643106242 24-25 28.84521323555021 26-27 30.295681380068533 28-29 27.118840412918154 30-31 29.1699539502675 32-33 28.058536614913088 34-35 25.96169134716289 36-37 29.113773579477055 38-39 29.31532633829669 40-41 26.142573969559628 42-43 27.05826858457266 44-45 25.635739195878692 46-47 29.19986129051309 48-49 24.298843229508172 50-51 29.84593555460323 52-53 23.474536360511408 54-55 24.66901281548458 56-57 26.90161469351412 58-59 26.133563910093233 60-61 25.64202352306954 62-63 25.446603661869883 64-65 23.89914487721333 66-67 24.42157689669323 68-69 25.48408248065866 70-71 23.895207708370876 72-73 23.779969804943573 74-75 20.842084700616166 76-77 23.306146677710927 78-79 22.38484916857594 80-81 23.4490961926063 82-83 24.415065425146093 84-85 21.188252699610675 86-87 22.813546283691185 88-89 21.629442754322177 90-91 22.746538698583983 92-93 22.747598705580028 94-95 21.70402181797257 96-97 20.627736143058545 98-99 20.043445143880806 100-101 21.68168595627017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 660373.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.85995793448097 #Duplication Level Percentage of deduplicated Percentage of total 1 95.52469797514446 82.97271247825047 2 2.965654346641553 5.151932235949919 3 0.6671505378199879 1.738460029530315 4 0.2842584849769166 0.9876272019045707 5 0.16602504496470374 0.721046421085224 6 0.09883717769069818 0.5150995857946118 7 0.06667818072404366 0.40541647809867076 8 0.055271929316109934 0.38407339642839317 9 0.034640227499071904 0.2707963833069213 >10 0.13395641246610177 1.7332087710945359 >50 0.0019454242687076274 0.10799277710041663 >100 3.5370339506455846E-4 0.0649784301650879 >500 3.537033950645283E-4 0.1697427076416489 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.7685169753226416E-4 4.77691310364919 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 31097 4.709005365149696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.571478543186957E-5 0.0 12-13 0.0 0.0 0.0 1.5142957086373914E-4 0.0 14-15 0.0 0.0 0.0 1.5142957086373914E-4 0.0 16-17 0.0 0.0 0.0 1.5142957086373914E-4 0.0 18-19 0.0 0.0 0.0 1.5142957086373914E-4 0.0 20-21 0.0 0.0 0.0 1.5142957086373914E-4 0.0 22-23 0.0 0.0 0.0 2.2714435629560872E-4 0.0 24-25 0.0 0.0 0.0 4.5428871259121744E-4 0.0 26-27 0.0 0.0 0.0 0.001060006996046174 0.0 28-29 0.0 0.0 0.0 0.003180020988138522 0.0 30-31 0.0 0.0 0.0 0.006890045474300131 0.0 32-33 0.0 0.0 0.0 0.016354393653283825 0.0 34-35 0.0 0.0 0.0 0.03180020988138522 0.0 36-37 0.0 0.0 0.0 0.056786089073902174 0.0 38-39 0.0 0.0 0.0 0.08646628496319504 0.0 40-41 0.0 0.0 0.0 0.11440504078755492 0.0 42-43 0.0 0.0 0.0 0.1458266767417808 0.0 44-45 0.0 0.0 0.0 0.18868124529621894 0.0 46-47 0.0 7.571478543186957E-5 0.0 0.242060169025687 0.0 48-49 0.0 1.5142957086373914E-4 0.0 0.29248621612331216 0.0 50-51 0.0 1.5142957086373914E-4 0.0 0.3460922842090758 0.0 52-53 0.0 2.2714435629560872E-4 0.0 0.40931413004468686 7.571478543186957E-5 54-55 0.0 3.0285914172747827E-4 0.0 0.4931303975177665 1.5142957086373914E-4 56-57 0.0 3.0285914172747827E-4 0.0 0.5823224147565088 1.5142957086373914E-4 58-59 0.0 3.0285914172747827E-4 0.0 0.6787830513967106 1.5142957086373914E-4 60-61 0.0 3.0285914172747827E-4 0.0 0.7710793748381597 1.5142957086373914E-4 62-63 0.0 3.7857392715934783E-4 0.0 0.8776100779408 1.5142957086373914E-4 64-65 0.0 4.542887125912174E-4 0.0 0.999586597271542 1.5142957086373914E-4 66-67 0.0 4.542887125912174E-4 1.5142957086373914E-4 1.1368575032595216 1.5142957086373914E-4 68-69 0.0 4.542887125912174E-4 1.5142957086373914E-4 1.280488451223778 1.5142957086373914E-4 70-71 0.0 4.542887125912174E-4 1.5142957086373914E-4 1.4360066205008382 1.5142957086373914E-4 72-73 0.0 4.542887125912174E-4 1.5142957086373914E-4 1.6009134231714501 1.5142957086373914E-4 74-75 0.0 4.542887125912174E-4 1.5142957086373914E-4 1.7833103412768239 1.5142957086373914E-4 76-77 0.0 4.542887125912174E-4 1.5142957086373914E-4 1.983954522671278 1.5142957086373914E-4 78-79 0.0 4.542887125912174E-4 1.5142957086373914E-4 2.2114017381086146 1.5142957086373914E-4 80-81 0.0 4.542887125912174E-4 1.5142957086373914E-4 2.44732900951432 1.5142957086373914E-4 82-83 0.0 4.542887125912174E-4 1.5142957086373914E-4 2.6887077454711203 1.5142957086373914E-4 84-85 0.0 4.542887125912174E-4 1.5142957086373914E-4 2.941897987955292 1.5142957086373914E-4 86-87 0.0 4.542887125912174E-4 1.5142957086373914E-4 3.225601288968507 1.5142957086373914E-4 88-89 0.0 4.542887125912174E-4 1.5142957086373914E-4 3.526340416703893 1.5142957086373914E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 530 0.0 56.437477 1 GTATCAA 990 0.0 49.488453 1 TCAACGC 1235 0.0 39.396988 4 ATCAACG 1240 0.0 38.838512 3 CAACGCA 1245 0.0 38.664387 5 TATCAAC 1255 0.0 38.618546 2 GTACTAC 85 9.277184E-6 36.403893 1 AACGCAG 1390 0.0 34.271877 6 ACGCAGA 1585 0.0 29.126364 7 GTGGTAT 200 2.746674E-10 28.364698 1 CGCAGAG 1655 0.0 27.423622 8 GCAGAGT 1730 0.0 25.803019 9 ATACCGT 95 0.0013523132 24.580963 6 CAGAGTA 1675 0.0 21.37044 10-11 CTACGAC 120 0.0032642132 21.122702 3 TGGTATC 285 1.6600097E-8 19.9003 2 GAGTACT 1355 0.0 19.85589 12-13 GTATATG 185 8.080281E-5 19.513798 1 AGAGTAC 1565 0.0 18.79334 10-11 GTATAGG 140 0.0072085946 18.418634 1 >>END_MODULE