FastQCFastQC Report
Sat 18 Jun 2016
SRR1930984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660373
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.2927133604796078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.2796904173853262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.15915247897778984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.055.3337551
GTATCAA19450.045.2087481
GGACCGT459.696296E-442.1563646
TATCAAC23400.037.37142
CAACGCA23450.037.0104145
ATCAACG23500.037.0073243
TCAACGC23800.036.5408444
AACGCAG24300.035.715816
TGGTATC5050.031.9981562
GTGGTAT5200.031.9915331
ACGCAGA28150.030.157157
CGCAGAG28300.029.9973078
GCAGAGT32650.025.1291949
CGTAGTC1050.002189049522.6300323
GTACATG13000.022.302671
AGAGCCG1100.002902251421.5572325
CAGAGTA30400.021.52886610-11
GAGTACT24800.021.51377112-13
TACATGG13200.021.2429812
AGAGTAC29200.019.89614510-11
Produced by FastQC (version 0.11.5)