##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930984_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 660373 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30407966406864 31.0 31.0 34.0 30.0 34.0 2 31.476312629377638 31.0 31.0 34.0 30.0 34.0 3 31.529540123536243 31.0 31.0 34.0 30.0 34.0 4 35.322320870174885 37.0 35.0 37.0 33.0 37.0 5 35.168214024498276 37.0 35.0 37.0 32.0 37.0 6 35.18465473300695 37.0 35.0 37.0 32.0 37.0 7 35.048636149570015 37.0 35.0 37.0 32.0 37.0 8 35.06151220598056 37.0 35.0 37.0 32.0 37.0 9 36.54463462315994 39.0 35.0 39.0 32.0 39.0 10-11 36.51108767317864 38.0 35.0 39.0 32.0 39.0 12-13 36.41019999303424 38.0 35.0 39.0 32.0 39.0 14-15 37.45374205184039 39.0 36.0 41.0 32.0 41.0 16-17 37.40558593401002 39.0 36.0 41.0 32.0 41.0 18-19 37.44272403626435 39.0 36.0 41.0 32.0 41.0 20-21 37.430069067027276 39.0 36.0 41.0 32.0 41.0 22-23 37.32786243532064 39.0 36.0 40.5 31.5 41.0 24-25 37.19078838777479 39.0 36.0 40.5 31.0 41.0 26-27 36.930418112188114 39.0 36.0 40.0 31.0 41.0 28-29 36.73353241274249 39.0 35.5 40.0 30.0 41.0 30-31 36.50375166761815 38.0 35.0 40.0 30.0 41.0 32-33 36.330116464482956 38.0 35.0 40.0 30.0 41.0 34-35 36.39646381666119 38.0 35.0 40.0 30.0 41.0 36-37 36.43200433694291 38.0 35.0 40.0 30.0 41.0 38-39 36.40908092850556 38.5 35.0 40.0 30.0 41.0 40-41 36.281347814038426 38.5 35.0 40.0 30.0 41.0 42-43 36.12863260611806 38.0 35.0 40.0 30.0 41.0 44-45 35.95105644840113 38.0 35.0 40.0 29.0 41.0 46-47 35.69963490330465 38.0 35.0 40.0 28.0 41.0 48-49 35.46679300940529 38.0 34.0 40.0 27.0 41.0 50-51 35.19983176174677 38.0 34.0 40.0 26.5 41.0 52-53 34.97708340589334 38.0 34.0 40.0 26.0 41.0 54-55 34.824022938551394 38.0 34.0 40.0 26.0 41.0 56-57 34.65301730991425 38.0 33.0 40.0 26.0 41.0 58-59 34.43394187830211 37.5 33.0 40.0 26.0 41.0 60-61 34.120923326665384 37.0 33.0 40.0 25.0 41.0 62-63 33.85486762784063 37.0 33.0 40.0 24.5 41.0 64-65 33.53139740722288 36.0 32.0 39.0 23.0 41.0 66-67 33.20987684233002 36.0 32.0 39.0 23.0 40.5 68-69 32.850679843058394 35.5 31.5 39.0 22.0 40.0 70-71 32.473370352815756 35.0 31.0 38.0 22.0 40.0 72-73 32.057600023017294 35.0 31.0 37.5 21.5 40.0 74-75 31.55955043589002 35.0 31.0 37.0 20.0 39.0 76-77 30.136622787424685 33.5 28.5 35.5 18.5 38.5 78-79 30.893704769880053 34.0 30.0 36.0 20.0 38.5 80-81 30.871886040162153 34.0 30.0 36.0 20.0 37.5 82-83 30.620586395870212 34.0 30.0 35.0 19.0 37.0 84-85 30.324335640615228 34.0 30.0 35.0 18.0 37.0 86-87 30.009216003682766 34.0 30.0 35.0 17.0 36.0 88-89 29.682475661482222 34.0 29.5 35.0 11.5 36.0 90-91 29.36611430206868 34.0 29.0 35.0 7.0 35.5 92-93 29.086005181919916 34.0 29.0 35.0 3.5 35.0 94-95 28.732304318922793 34.0 29.0 35.0 2.0 35.0 96-97 28.338652549392542 34.0 29.0 35.0 2.0 35.0 98-99 27.876768886674654 33.5 29.0 35.0 2.0 35.0 100-101 26.778468380748457 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3511530181613054 1101 2 0.3447772433212002 1101 3 0.3410492983898905 1101 4 0.2170748358675212 1101 5 0.22405362988163802 1101 6 0.37570334747231016 1101 7 0.6023630324278315 1101 8 0.5265464949569676 1101 9 0.4794880889221744 1101 10-11 0.4969331856499366 1101 12-13 0.41502052932843725 1101 14-15 0.7078140386588672 1101 16-17 0.692608716628321 1101 18-19 0.5812975472354154 1101 20-21 0.6245992604604069 1101 22-23 0.6225969551579524 1101 24-25 0.4979998116264781 1101 26-27 0.619316113187864 1101 28-29 0.4557907680616964 1101 30-31 0.8159011421939582 1101 32-33 0.7420166980732219 1101 34-35 0.7348921246438849 1101 36-37 0.9616738370304603 1101 38-39 1.0722359071663234 1101 40-41 0.7596918471063745 1101 42-43 0.7106116731786116 1101 44-45 0.5707402580991499 1101 46-47 0.9338123173449375 1101 48-49 0.9241347393031702 1101 50-51 0.7622621706890413 1101 52-53 0.5728313137840573 1101 54-55 0.5755684172395661 1101 56-57 0.7676905323381931 1101 58-59 0.6281139174527226 1101 60-61 0.6518351594011094 1101 62-63 0.6710490207022772 1101 64-65 1.0097832803886604 1101 66-67 0.816488017181463 1101 68-69 0.8112664810584604 1101 70-71 0.5790144109030493 1101 72-73 0.7775329645725435 1101 74-75 1.0022331092513674 1101 76-77 0.6559435988714419 1101 78-79 0.6878674681853276 1101 80-81 1.0125945470616884 1101 82-83 0.9433441279775678 1101 84-85 1.0168999440621107 1101 86-87 1.3513716167393426 1101 88-89 1.079756936751867 1101 90-91 0.912500883480778 1101 92-93 0.8056311787094081 1101 94-95 0.9978275370242393 1101 96-97 1.0707873509216306 1101 98-99 0.9240757631577985 1101 100-101 1.006427552294216 1102 1 0.3131331786011806 1102 2 0.24508960868095997 1102 3 0.34256496282024784 1102 4 0.17337958956564137 1102 5 0.1973999912611717 1102 6 0.16455154508827974 1102 7 0.24496628225857364 1102 8 0.25159317024060357 1102 9 0.494283128006316 1102 10-11 0.43157368338285096 1102 12-13 0.3362457298782786 1102 14-15 0.47173327503070794 1102 16-17 0.6167562515578453 1102 18-19 0.5087469737962138 1102 20-21 0.48714696157446014 1102 22-23 0.4205763257084172 1102 24-25 0.4939990730285828 1102 26-27 0.52642973416404 1102 28-29 0.42907942648069053 1102 30-31 0.42292474655169343 1102 32-33 0.611106632625237 1102 34-35 0.5668675149723583 1102 36-37 0.6865974126649377 1102 38-39 0.7754554759072221 1102 40-41 0.868178542085964 1102 42-43 0.9329860602449429 1102 44-45 0.7430066765148595 1102 46-47 0.7070743041732683 1102 48-49 0.9820864637240874 1102 50-51 0.9596255300784691 1102 52-53 0.7986460795869803 1102 54-55 0.9246674816822349 1102 56-57 0.977066365825408 1102 58-59 1.1582540971782151 1102 60-61 1.0788627132059148 1102 62-63 0.5618583496040159 1102 64-65 0.6422115748430244 1102 66-67 0.5302915911746169 1102 68-69 0.650377804174525 1102 70-71 0.6861175171175304 1102 72-73 0.7507985418073204 1102 74-75 0.8002329964100205 1102 76-77 0.8421036078987463 1102 78-79 1.0616582705898772 1102 80-81 0.7597273510665303 1102 82-83 0.7901427677264437 1102 84-85 0.8882967147479803 1102 86-87 0.7957433981964321 1102 88-89 0.9347468323223325 1102 90-91 0.9531199208415231 1102 92-93 1.085318710766611 1102 94-95 1.1505134174944764 1102 96-97 1.4085343195927678 1102 98-99 1.099395309330422 1102 100-101 1.2659818802727152 1103 1 0.22839215833148074 1103 2 0.4039465223180656 1103 3 0.3403215778345867 1103 4 0.2147237386888392 1103 5 0.35901780363891334 1103 6 0.24012341016823058 1103 7 0.32566128589849797 1103 8 0.31181295263721864 1103 9 0.5722444704716594 1103 10-11 0.32846587709642705 1103 12-13 0.4783602068922548 1103 14-15 0.5741093812473181 1103 16-17 0.5027856087017639 1103 18-19 0.5574786936751863 1103 20-21 0.5481046343968146 1103 22-23 0.6238564715036787 1103 24-25 0.6397373843985292 1103 26-27 0.6550863774063984 1103 28-29 0.6346140999022722 1103 30-31 0.5487437306399983 1103 32-33 0.8027253859370802 1103 34-35 0.4973473418404808 1103 36-37 0.5987092154451474 1103 38-39 0.5936756742957456 1103 40-41 0.7705516769317242 1103 42-43 0.7810326315306 1103 44-45 0.4585313463212728 1103 46-47 0.6434238265254066 1103 48-49 0.46751808010030516 1103 50-51 0.25977112724972073 1103 52-53 0.43252119287048885 1103 54-55 0.48978135946975243 1103 56-57 0.8277834566552613 1103 58-59 0.6487419962704593 1103 60-61 0.6101871522358593 1103 62-63 0.49009850570003266 1103 64-65 0.17981798706130547 1103 66-67 -0.04378963619963372 1103 68-69 -0.1725557312120216 1103 70-71 0.026646520173088106 1103 72-73 0.19443287500693884 1103 74-75 0.2157631137296505 1103 76-77 0.226980320770231 1103 78-79 0.4551928152520581 1103 80-81 0.4528095045180258 1103 82-83 0.36433046922253 1103 84-85 0.43673872596985674 1103 86-87 0.5059282669856451 1103 88-89 0.38374820410520627 1103 90-91 0.39181682615879154 1103 92-93 0.2478613623189041 1103 94-95 0.2776360905396871 1103 96-97 0.5791281480555313 1103 98-99 0.7485271738161039 1103 100-101 1.040546450637251 1104 1 0.27377535030564104 1104 2 0.35589946707620257 1104 3 0.37588415245843265 1104 4 0.30746661404381825 1104 5 0.288725111662373 1104 6 0.12315127132387715 1104 7 0.5243498089290242 1104 8 0.5319955224090265 1104 9 0.8260654493462738 1104 10-11 0.4697065238443656 1104 12-13 0.4349998860869988 1104 14-15 0.41125219761675424 1104 16-17 0.38062726105809475 1104 18-19 0.5231208036451633 1104 20-21 0.24744009564950176 1104 22-23 0.3723247501247542 1104 24-25 0.3486294551241045 1104 26-27 0.6188899460317998 1104 28-29 0.6248029963493451 1104 30-31 0.5722677838982335 1104 32-33 0.48639400134657507 1104 34-35 0.4048946373057305 1104 36-37 0.3649314292106638 1104 38-39 0.6236001347445139 1104 40-41 0.5721438109240466 1104 42-43 0.5303749902863899 1104 44-45 0.5530592479105536 1104 46-47 0.32852070076727813 1104 48-49 0.41845661621230335 1104 50-51 0.2733782359591288 1104 52-53 0.22086774245402552 1104 54-55 0.07932878812816568 1104 56-57 0.49093437878006796 1104 58-59 0.44435827628053914 1104 60-61 0.4274544566440781 1104 62-63 0.2942248774706897 1104 64-65 0.23821010773430373 1104 66-67 0.2798031542447106 1104 68-69 0.3739524673028711 1104 70-71 0.6354938760509512 1104 72-73 0.7999658617209207 1104 74-75 0.7299184550133511 1104 76-77 0.6406878000420448 1104 78-79 0.7155129069650634 1104 80-81 0.6288765958171822 1104 82-83 0.3701505347725913 1104 84-85 0.3614548580403927 1104 86-87 0.40304130516927117 1104 88-89 0.5572156502690042 1104 90-91 0.27348540351309936 1104 92-93 0.4075516406450497 1104 94-95 0.5429901702938515 1104 96-97 0.5905708678599275 1104 98-99 0.6217926896304142 1104 100-101 0.7618778114432594 1105 1 0.2585246347278982 1105 2 0.24049028212877488 1105 3 0.3204153902005906 1105 4 -0.023189700187266737 1105 5 -0.1772129395902482 1105 6 0.1590724894086577 1105 7 0.2799057629617039 1105 8 0.10402735973472943 1105 9 0.17184023456087516 1105 10-11 0.45212247568044006 1105 12-13 0.3418402937589846 1105 14-15 0.3990307798202153 1105 16-17 0.39906529549996606 1105 18-19 0.30608725162260697 1105 20-21 0.25810819982238087 1105 22-23 0.28246118620018024 1105 24-25 0.44308795833944004 1105 26-27 0.36945758914174576 1105 28-29 0.4776080386788166 1105 30-31 0.39692490056262386 1105 32-33 0.47185157884975126 1105 34-35 0.30067244845596974 1105 36-37 0.46645044889717724 1105 38-39 0.5781371329715412 1105 40-41 0.15042924855278983 1105 42-43 0.32304908805325283 1105 44-45 0.4472041745372124 1105 46-47 0.3148154537948926 1105 48-49 0.3326141845256032 1105 50-51 0.11418600387210631 1105 52-53 0.2152316571629811 1105 54-55 0.33630020760577395 1105 56-57 0.6372820744740579 1105 58-59 0.5873460627077662 1105 60-61 0.1771905278140835 1105 62-63 -0.10444662471582689 1105 64-65 0.20666865129545187 1105 66-67 0.09067321160299713 1105 68-69 -0.001436141282269432 1105 70-71 -0.005956286789214005 1105 72-73 0.27833266339661833 1105 74-75 0.28598231794518725 1105 76-77 0.48309595168848674 1105 78-79 0.42378581497886003 1105 80-81 0.5571282998672267 1105 82-83 0.364617555436368 1105 84-85 0.4627026642370602 1105 86-87 0.5433976483148548 1105 88-89 0.6589319161299478 1105 90-91 0.40780220328357686 1105 92-93 0.5594390399766525 1105 94-95 0.2876146516821052 1105 96-97 0.3867942760496774 1105 98-99 0.27559315271063767 1105 100-101 0.373980881535136 1106 1 0.2237778122462757 1106 2 0.21139698051615952 1106 3 0.20704650177647466 1106 4 0.08252133626977098 1106 5 0.2608358005133695 1106 6 0.21607610156187462 1106 7 0.26318267001737894 1106 8 0.4253129202720629 1106 9 0.39347786021283326 1106 10-11 0.23534208501016707 1106 12-13 0.42772261338007667 1106 14-15 0.48330200939020074 1106 16-17 0.37203732636666587 1106 18-19 0.6095103005587887 1106 20-21 0.20915132344168086 1106 22-23 0.29260375514262904 1106 24-25 0.10067958023544321 1106 26-27 0.18608525849683133 1106 28-29 0.35707366657911166 1106 30-31 0.30633567079220825 1106 32-33 0.4595946584609152 1106 34-35 0.31143265638353057 1106 36-37 -0.0016828652642928432 1106 38-39 0.11206370473748706 1106 40-41 0.03326193483575679 1106 42-43 0.08930377753443253 1106 44-45 -0.014305043207208712 1106 46-47 -0.025198620095210345 1106 48-49 -0.2356888441929783 1106 50-51 -0.1842741469755964 1106 52-53 -0.018889465436721764 1106 54-55 -0.3284536300346872 1106 56-57 -0.12054788853296827 1106 58-59 0.02091120128983448 1106 60-61 0.010816885639478357 1106 62-63 -0.022278853488430173 1106 64-65 -0.18162910516088715 1106 66-67 -0.026307830039158375 1106 68-69 -0.16862567693103614 1106 70-71 -0.10987431836178274 1106 72-73 0.1303683666336113 1106 74-75 0.04798216108449438 1106 76-77 -0.22358653176488374 1106 78-79 -0.12908604290534598 1106 80-81 -0.3528423758973567 1106 82-83 0.029059850308332358 1106 84-85 0.26094277438812696 1106 86-87 0.30073088578887663 1106 88-89 0.20637791901444302 1106 90-91 0.31594828416269394 1106 92-93 0.2672064765678961 1106 94-95 0.25366738301895 1106 96-97 0.12247202318152262 1106 98-99 -0.1272784873114965 1106 100-101 -0.13881197956036218 1107 1 0.22136543286524457 1107 2 0.3090170995102106 1107 3 0.272693219440594 1107 4 0.08533369565180493 1107 5 0.2619232461410874 1107 6 0.2369000603639364 1107 7 0.04193123258925624 1107 8 0.21106113286143113 1107 9 0.09469279946586795 1107 10-11 0.288576923268252 1107 12-13 0.2112959347140091 1107 14-15 0.20017866555739516 1107 16-17 0.12706832481674013 1107 18-19 0.1752195150980569 1107 20-21 0.3810239798088588 1107 22-23 0.38228677099183983 1107 24-25 0.2548968693709881 1107 26-27 0.4695710842055476 1107 28-29 0.45869358558315554 1107 30-31 0.3835005625476171 1107 32-33 0.2781849789448927 1107 34-35 0.22179963803444025 1107 36-37 0.23025553781555175 1107 38-39 0.5358612648594772 1107 40-41 0.3741261416254176 1107 42-43 0.28723138115424973 1107 44-45 0.21273985454789823 1107 46-47 0.19064058794438665 1107 48-49 0.44759574157773585 1107 50-51 0.6219042941023503 1107 52-53 0.5150088396480257 1107 54-55 0.6331982873694386 1107 56-57 0.42249370938714037 1107 58-59 0.333351829258433 1107 60-61 0.6265673481163532 1107 62-63 0.5360932279075001 1107 64-65 0.34121350270871886 1107 66-67 0.6136851703108235 1107 68-69 0.49010388659078075 1107 70-71 0.709228476498474 1107 72-73 0.8610818309603943 1107 74-75 1.0087403731738895 1107 76-77 0.47172245278588676 1107 78-79 0.7002537401054987 1107 80-81 0.32441126142077437 1107 82-83 0.537990653768162 1107 84-85 0.7846103410097953 1107 86-87 0.9256433901636569 1107 88-89 0.9961071825512668 1107 90-91 1.1294326099464023 1107 92-93 0.8975124612686578 1107 94-95 1.0997563285716723 1107 96-97 1.1120412947179474 1107 98-99 0.8440166979292378 1107 100-101 0.9949125842114519 1108 1 0.08133841247713391 1108 2 -0.019550819919906814 1108 3 0.03312063285252975 1108 4 0.08947272783063198 1108 5 0.18505024196520736 1108 6 0.027811384362955494 1108 7 0.0010690365686158998 1108 8 0.3936084185406301 1108 9 0.3943243396279925 1108 10-11 0.1518291954372586 1108 12-13 0.17222690990437428 1108 14-15 0.07333022537070377 1108 16-17 0.15091381732472087 1108 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-1.0852263892464933 2316 92-93 -0.3733460940178652 2316 94-95 -0.7955977876510865 2316 96-97 -0.6870860257017455 2316 98-99 -0.5812002108681789 2316 100-101 -1.5861860840694213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 6.0 5 21.0 6 37.0 7 103.0 8 264.0 9 513.0 10 950.0 11 1422.0 12 1994.0 13 2508.0 14 3075.0 15 3802.0 16 4581.0 17 5049.0 18 4281.0 19 3333.0 20 3230.0 21 3346.0 22 3655.0 23 4459.0 24 5311.0 25 6956.0 26 8977.0 27 11638.0 28 15095.0 29 18926.0 30 22870.0 31 27672.0 32 33198.0 33 40231.0 34 50026.0 35 62746.0 36 81598.0 37 110952.0 38 100286.0 39 17258.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.57695417912211 22.054988687885587 15.310727276581954 23.057329856410348 2 15.659585294491796 22.319346260320692 39.13018457605661 22.890883869130914 3 18.158153368092204 25.83776400412503 31.96125137993122 24.04283124785155 4 13.07187063891754 18.668315029427827 38.17517140950185 30.08464292215278 5 12.645923529937322 38.100862707329156 35.98475383993349 13.268459922800035 6 30.00507295207953 38.70844154369175 17.47896996358075 13.807515540647975 7 25.34158725447588 34.00169298260226 22.99927465235556 17.6574451105663 8 24.24500315733524 36.21834525347269 21.099328100889352 18.437323488302717 9 24.767063462618854 16.36544801195688 22.107960198251593 36.759528327172674 10-11 23.60749152372977 27.199400944617665 29.369992413378498 19.823115118274064 12-13 24.03149432214824 25.126708693420234 29.45926014540267 21.38253683902885 14-15 21.608772617899277 27.12474616618184 27.153290640289658 24.11319057562923 16-17 20.70072519621487 29.77029648395679 28.45210206958794 21.076876250240396 18-19 20.551945642841243 28.58990297907395 30.637079347580837 20.221072030503972 20-21 21.20248707927187 27.773091873834936 30.700831196914468 20.323589849978724 22-23 21.068579527902457 27.53826631050378 30.53854494133609 20.85460922025767 24-25 21.123704229223183 27.823059665838596 30.46339965731307 20.589836447625157 26-27 20.97576303654852 27.955493635998817 30.385550196485223 20.683193130967435 28-29 20.87464205835187 27.932547211954457 30.56106170300724 20.631749026686432 30-31 21.13452450632601 27.772044108282305 30.362038951765747 20.73139243362594 32-33 21.093893179487726 27.89744164524733 30.131742168247683 20.87692300701726 34-35 21.27694698293749 27.801017147322792 30.21492462265894 20.707111247080775 36-37 21.33089328155416 27.643732249494036 30.202227998458437 20.823146470493366 38-39 21.10701073484228 27.80761781599187 30.254189677651873 20.831181771513975 40-41 21.234549205928563 27.728709610251943 30.298586003899374 20.73815517992012 42-43 21.30604567479708 27.95099489094705 29.754724567752312 20.988234866503557 44-45 21.155993993853013 28.025657121092916 29.709528738565265 21.108820146488803 46-47 21.380849866765814 27.9393491023446 29.467282847963094 21.212518182926487 48-49 21.30839932576408 28.10126563108149 29.35621979606332 21.234115247091108 50-51 21.26333979651595 28.31731192290303 29.217074939540417 21.202273341040602 52-53 21.422101140297617 28.286703400577768 28.981054671066246 21.31014078805837 54-55 21.33407445215013 28.357866486536427 29.091892170241252 21.216166891072195 56-57 21.263172401051296 28.413996345763742 29.007639367921623 21.315191885263342 58-59 21.173274850834034 28.338845096852804 29.124072611394546 21.363807440918617 60-61 21.151542718520204 28.44772951254643 29.21461602607839 21.186111742854976 62-63 21.11731283879067 28.277247080575062 29.212920305353705 21.392519775280558 64-65 20.948182489569174 28.36122350926649 29.119484109544747 21.57110989161959 66-67 20.962391563759144 28.351085956501016 29.077081201550975 21.609441278188868 68-69 21.04429779216974 28.246503345220365 29.14546964404183 21.563729218568064 70-71 21.010828845348183 28.076141931644997 29.14005245933491 21.77297676367191 72-73 20.99633414072228 28.252141956874205 28.99615820374412 21.75536569865939 74-75 21.04531098153547 28.478043002915456 28.80489249271137 21.671753522837705 76-77 20.87962197211225 28.514155560175357 28.794353253950312 21.811869213762083 78-79 20.827777437041377 28.550681510658617 28.73785937278758 21.883681679512428 80-81 20.92313120483606 28.518168547424782 28.626895143949167 21.931805103789987 82-83 20.897507923552293 28.30158107614939 28.888976381529837 21.911934618768484 84-85 20.87259775914521 28.341634197642847 28.717936681239237 22.067831361972704 86-87 20.67937362672308 28.435974820290955 28.820984504212017 22.06366704877395 88-89 20.73331395031891 28.278152314150205 28.67345980750244 22.315073928028443 90-91 20.60201170313295 28.218740156526373 28.788601560417725 22.390646579922947 92-93 20.65503182089426 28.196780652411636 28.7778413892751 22.37034613741901 94-95 20.43325181152562 28.024623119382756 28.917249055432304 22.62487601365932 96-97 20.352074018830283 28.000257430465382 28.926552816781438 22.7211157339229 98-99 20.29951254821139 28.064366653391343 28.76699986219909 22.86912093619818 100-101 20.26506232083989 27.951627337883288 28.657364852893743 23.12594548838308 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 104.0 1 83.0 2 83.0 3 104.0 4 81.5 5 94.0 6 207.5 7 405.5 8 629.0 9 769.5 10 683.0 11 500.0 12 456.5 13 486.0 14 559.5 15 631.5 16 704.5 17 806.5 18 874.5 19 976.0 20 1138.5 21 1305.5 22 1568.5 23 1935.5 24 2372.0 25 3010.5 26 4014.0 27 5281.5 28 6481.5 29 8023.0 30 9814.5 31 11499.0 32 13503.0 33 15403.0 34 17141.5 35 18563.0 36 20125.5 37 21912.0 38 23122.5 39 23951.0 40 24491.0 41 24851.0 42 25102.0 43 25846.0 44 26648.0 45 27173.5 46 27262.0 47 27000.0 48 26956.0 49 26446.5 50 25188.5 51 23653.0 52 22082.0 53 20607.0 54 18875.5 55 16346.5 56 13596.5 57 10870.5 58 8400.0 59 6407.0 60 4579.0 61 3170.0 62 2206.0 63 1430.5 64 815.0 65 445.0 66 270.0 67 143.0 68 73.5 69 40.0 70 18.5 71 10.0 72 5.0 73 2.0 74 2.5 75 3.0 76 2.5 77 2.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0028771618464110437 2 0.007420048972323217 3 0.0021200139920923475 4 0.1715697037886164 5 0.0015142957086373913 6 0.001211436566909913 7 0.0 8 9.085774251824348E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5142957086373914E-4 24-25 5.300034980230869E-4 26-27 0.0027257322755473044 28-29 0.0 30-31 0.006587186332572652 32-33 0.021805858204378435 34-35 0.014385809232055218 36-37 6.814330688868261E-4 38-39 0.0 40-41 0.0015900104940692608 42-43 0.011281503029348565 44-45 0.007495763757755088 46-47 0.01052435517502987 48-49 0.01060006996046174 50-51 0.1900441114339926 52-53 0.11576790692532857 54-55 0.016657252795011304 56-57 0.006587186332572652 58-59 0.017944404147353085 60-61 0.12462653682085731 62-63 0.24168159509852763 64-65 0.2466030561515992 66-67 0.2560674043305829 68-69 0.2531902424841718 70-71 0.23880443325211662 72-73 0.15839533112347112 74-75 0.27454181197595906 76-77 0.20806423036677757 78-79 0.0058300384782539565 80-81 1.5142957086373914E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.5142957086373914E-4 90-91 0.005602894121958348 92-93 0.01741440064933 94-95 0.0027257322755473044 96-97 7.571478543186957E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 660373.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.88893180692367 #Duplication Level Percentage of deduplicated Percentage of total 1 84.12028991688369 61.314380753303844 2 10.681912328929059 15.571863586216947 3 2.4244229355074367 5.301407940520297 4 0.9602720670100808 2.7997282083356563 5 0.47502670793580826 1.7312094660600283 6 0.2883208885387175 1.260924094992613 7 0.2135386688001879 1.0895223827822706 8 0.1462716771264329 0.852926903948234 9 0.11174330005913675 0.7330364800101925 >10 0.5494689291062035 6.899858921463023 >50 0.02331925718584238 1.1539824809953552 >100 0.004580504007444568 0.4784936105928851 >500 2.0820472747230597E-4 0.0795212977568661 >1k 6.24614182416918E-4 0.7331438730217942 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1933 0.2927133604796078 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1847 0.2796904173853262 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1051 0.15915247897778984 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.5142957086373914E-4 3 0.0 0.0 0.0 0.0 1.5142957086373914E-4 4 0.0 0.0 0.0 0.0 1.5142957086373914E-4 5 0.0 0.0 0.0 0.0 1.5142957086373914E-4 6 0.0 0.0 0.0 0.0 1.5142957086373914E-4 7 0.0 0.0 0.0 0.0 1.5142957086373914E-4 8 0.0 0.0 0.0 0.0 1.5142957086373914E-4 9 0.0 0.0 0.0 0.0 1.5142957086373914E-4 10-11 0.0 0.0 0.0 0.0 1.5142957086373914E-4 12-13 0.0 0.0 0.0 0.0 1.5142957086373914E-4 14-15 0.0 0.0 0.0 0.0 3.0285914172747827E-4 16-17 0.0 0.0 0.0 0.0 3.0285914172747827E-4 18-19 0.0 0.0 0.0 0.0 3.0285914172747827E-4 20-21 0.0 0.0 0.0 0.0 3.0285914172747827E-4 22-23 0.0 0.0 0.0 2.2714435629560872E-4 3.0285914172747827E-4 24-25 0.0 0.0 0.0 4.5428871259121744E-4 3.0285914172747827E-4 26-27 0.0 0.0 0.0 0.0012871513523417828 3.0285914172747827E-4 28-29 0.0 0.0 0.0 0.004012883627889086 3.0285914172747827E-4 30-31 0.0 0.0 0.0 0.011811506527371653 3.0285914172747827E-4 32-33 0.0 0.0 0.0 0.03217878380854457 3.0285914172747827E-4 34-35 0.0 0.0 0.0 0.0636761345482023 3.0285914172747827E-4 36-37 0.0 0.0 0.0 0.1122093120100307 3.0285914172747827E-4 38-39 0.0 0.0 0.0 0.17331114385354945 3.0285914172747827E-4 40-41 0.0 0.0 0.0 0.2381230001832298 3.0285914172747827E-4 42-43 0.0 0.0 0.0 0.30914346891832345 3.0285914172747827E-4 44-45 0.0 0.0 0.0 0.4058312499148209 3.0285914172747827E-4 46-47 0.0 0.0 0.0 0.5226591638361956 3.0285914172747827E-4 48-49 0.0 0.0 0.0 0.6306284478620416 3.0285914172747827E-4 50-51 0.0 0.0 0.0 0.740112027596525 3.0285914172747827E-4 52-53 0.0 0.0 0.0 0.8527756283191469 3.0285914172747827E-4 54-55 0.0 0.0 0.0 0.9982994459192003 3.0285914172747827E-4 56-57 0.0 0.0 0.0 1.1619948120229022 3.0285914172747827E-4 58-59 0.0 0.0 0.0 1.3371231107268164 3.0285914172747827E-4 60-61 0.0 0.0 0.0 1.5082385258028417 3.0285914172747827E-4 62-63 0.0 0.0 0.0 1.6967683415281969 4.542887125912174E-4 64-65 0.0 0.0 0.0 1.9133126278633439 4.542887125912174E-4 66-67 0.0 0.0 0.0 2.163549993715673 4.542887125912174E-4 68-69 0.0 0.0 0.0 2.4299146088649897 4.542887125912174E-4 70-71 0.0 0.0 0.0 2.713845054234501 4.542887125912174E-4 72-73 0.0 0.0 0.0 3.014962755897046 4.542887125912174E-4 74-75 0.0 0.0 0.0 3.3500763962185007 4.542887125912174E-4 76-77 0.0 0.0 0.0 3.7228959996850266 4.542887125912174E-4 78-79 0.0 0.0 0.0 4.149700245164475 4.542887125912174E-4 80-81 0.0 0.0 0.0 4.595130327860163 4.542887125912174E-4 82-83 0.0 0.0 0.0 5.0585048147032055 4.542887125912174E-4 84-85 0.0 0.0 0.0 5.54951519822888 4.542887125912174E-4 86-87 0.0 0.0 0.0 6.095721660334387 4.542887125912174E-4 88-89 0.0 0.0 0.0 6.683571254427422 4.542887125912174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1005 0.0 55.333755 1 GTATCAA 1945 0.0 45.208748 1 GGACCGT 45 9.696296E-4 42.156364 6 TATCAAC 2340 0.0 37.3714 2 CAACGCA 2345 0.0 37.010414 5 ATCAACG 2350 0.0 37.007324 3 TCAACGC 2380 0.0 36.540844 4 AACGCAG 2430 0.0 35.71581 6 TGGTATC 505 0.0 31.998156 2 GTGGTAT 520 0.0 31.991533 1 ACGCAGA 2815 0.0 30.15715 7 CGCAGAG 2830 0.0 29.997307 8 GCAGAGT 3265 0.0 25.129194 9 CGTAGTC 105 0.0021890495 22.630032 3 GTACATG 1300 0.0 22.30267 1 AGAGCCG 110 0.0029022514 21.557232 5 CAGAGTA 3040 0.0 21.528866 10-11 GAGTACT 2480 0.0 21.513771 12-13 TACATGG 1320 0.0 21.242981 2 AGAGTAC 2920 0.0 19.896145 10-11 >>END_MODULE