FastQCFastQC Report
Sat 18 Jun 2016
SRR1930983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006865
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1353224.500434838278406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.057.652591
GTATCAA46300.045.9558261
ATCAACG54300.038.0554243
TCAACGC55650.037.491694
CAACGCA56700.036.3466035
TATCAAC58600.035.337462
AACGCAG60450.033.7491876
ACGCAGA67750.028.7042887
CGCAGAG69300.027.902598
GTACATG46950.025.66291
GCAGAGT75450.024.0971989
ACATGGG46450.024.0879483
GTGGTAT12800.024.0333731
TACATGG48250.023.902142
AGAGTAC66500.020.7278810-11
GAGTACT51200.020.64021312-13
TGGTATC12800.020.4226342
CAGAGTA72700.020.25147410-11
AGTACTT55950.019.31718812-13
CATGGGG28050.018.146314
Produced by FastQC (version 0.11.5)