FastQCFastQC Report
Sat 18 Jun 2016
SRR1930983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006865
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81070.26961636122672616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71220.23685798996629379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.14101065395353632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.059.0332871
GTATCAA80100.042.7146951
ATCAACG97550.034.53623
TCAACGC99200.034.1533624
CAACGCA98650.034.140135
TATCAAC104000.032.5324482
AACGCAG105300.032.2538346
ACGCAGA118900.028.1656347
CGCAGAG119200.028.015168
GCAGAGT138350.023.725959
GTACATG80150.022.8855361
GGACCGT2704.1836756E-1122.8389596
GCCGCGA1252.2781828E-422.7686828
ACATGGG74700.022.4548283
GTGGTAT20550.022.4304121
TACATGG79750.022.4040872
CAGAGTA133250.020.96740710-11
GAGTACT99600.020.95511412-13
AGAGTAC126150.020.79383310-11
TAACGCA2601.4861143E-820.1036534
Produced by FastQC (version 0.11.5)