##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930983_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3006865 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.376721269494972 31.0 31.0 34.0 30.0 34.0 2 31.544977243740572 31.0 31.0 34.0 30.0 34.0 3 31.611126538770446 31.0 31.0 34.0 30.0 34.0 4 35.37016261122465 37.0 35.0 37.0 33.0 37.0 5 35.25318562689046 37.0 35.0 37.0 33.0 37.0 6 35.282281712015674 37.0 35.0 37.0 32.0 37.0 7 35.16883864091005 37.0 35.0 37.0 32.0 37.0 8 35.173877776355106 37.0 35.0 37.0 32.0 37.0 9 36.683103830734005 39.0 35.0 39.0 32.0 39.0 10-11 36.632029040212984 39.0 35.0 39.0 32.0 39.0 12-13 36.51819885495358 38.0 35.0 39.0 32.0 39.0 14-15 37.589933036567984 39.0 36.0 41.0 32.0 41.0 16-17 37.55015406411661 39.0 36.0 41.0 32.0 41.0 18-19 37.584880431944896 39.0 36.0 41.0 32.0 41.0 20-21 37.57718071812336 39.0 36.0 41.0 32.0 41.0 22-23 37.479249151524925 39.0 36.0 41.0 32.0 41.0 24-25 37.35775334110444 39.0 36.0 41.0 31.5 41.0 26-27 37.11395606387383 39.0 36.0 40.0 31.0 41.0 28-29 36.93640303106392 39.0 36.0 40.0 31.0 41.0 30-31 36.71540607908902 38.5 35.5 40.0 30.0 41.0 32-33 36.55225558846173 38.0 35.0 40.0 30.0 41.0 34-35 36.61455718830077 38.5 35.0 40.0 30.0 41.0 36-37 36.679305855101575 39.0 35.0 40.0 30.0 41.0 38-39 36.664107467412066 39.0 35.0 40.0 30.0 41.0 40-41 36.555634855572166 39.0 35.0 40.0 30.0 41.0 42-43 36.41246198282929 39.0 35.0 40.0 30.0 41.0 44-45 36.24455953293547 39.0 35.0 40.0 30.0 41.0 46-47 35.998292241254596 38.5 35.0 40.0 29.0 41.0 48-49 35.77934792549715 38.5 35.0 40.0 28.5 41.0 50-51 35.522291822213504 38.0 34.5 40.0 27.5 41.0 52-53 35.322230462624695 38.0 34.0 40.0 27.0 41.0 54-55 35.18508961991975 38.0 34.0 40.0 27.0 41.0 56-57 35.026723680644125 38.0 34.0 40.0 26.0 41.0 58-59 34.83352195725448 38.0 34.0 40.0 26.0 41.0 60-61 34.535628802756364 38.0 33.0 40.0 26.0 41.0 62-63 34.292613569282295 37.0 33.0 40.0 26.0 41.0 64-65 33.9899539886227 37.0 33.0 40.0 25.0 41.0 66-67 33.68597060393466 36.5 32.0 39.0 24.0 41.0 68-69 33.34702888224114 36.0 32.0 39.0 23.0 40.5 70-71 32.98457380028701 36.0 31.5 39.0 22.5 40.0 72-73 32.575210892407874 35.0 31.0 38.5 22.0 40.0 74-75 32.06382112266431 35.0 31.0 37.5 21.0 39.5 76-77 30.6725044190544 33.5 29.5 36.0 20.0 39.0 78-79 31.39905765639628 34.0 30.5 36.0 20.5 39.0 80-81 31.357309855946312 34.0 31.0 36.0 21.0 39.0 82-83 31.079624625648307 34.0 31.0 36.0 20.0 37.5 84-85 30.731824508250288 34.0 30.5 35.5 19.5 37.0 86-87 30.382555086443855 34.0 30.0 35.0 18.0 37.0 88-89 30.026083478972286 34.0 30.0 35.0 17.5 36.0 90-91 29.68775252630231 34.0 29.5 35.0 11.5 36.0 92-93 29.37088312910623 34.0 29.0 35.0 7.0 35.5 94-95 28.99366948632546 34.0 29.0 35.0 2.0 35.0 96-97 28.575753650396678 34.0 29.0 35.0 2.0 35.0 98-99 28.093842922778375 34.0 29.0 35.0 2.0 35.0 100-101 26.979006373748074 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37543495329385124 1101 2 0.44305479077363685 1101 3 0.3283521194236876 1101 4 0.20650957439962525 1101 5 0.2637527641176334 1101 6 0.3314208485821979 1101 7 0.4315687238090433 1101 8 0.3295125753395638 1101 9 0.4835144057359315 1101 10-11 0.407794393347757 1101 12-13 0.3491936392184627 1101 14-15 0.46605820028641176 1101 16-17 0.6828308632120041 1101 18-19 0.5053848957027824 1101 20-21 0.5134039325919133 1101 22-23 0.4937987509316173 1101 24-25 0.457955709401368 1101 26-27 0.4759761252055199 1101 28-29 0.6143310221574865 1101 30-31 0.6091729585395669 1101 32-33 0.6231815269530969 1101 34-35 0.6251791351508373 1101 36-37 0.7218787844528478 1101 38-39 0.7076347463227393 1101 40-41 0.6899310885909813 1101 42-43 0.7851088028639666 1101 44-45 0.873999025405368 1101 46-47 0.7359772792436203 1101 48-49 0.7731008362301282 1101 50-51 0.6765146362955932 1101 52-53 0.7099743065055364 1101 54-55 0.6366434513293839 1101 56-57 0.7527195971103993 1101 58-59 0.7769936265611648 1101 60-61 0.7988811209209743 1101 62-63 0.5171020012985323 1101 64-65 0.6689772004430807 1101 66-67 0.6713658124632857 1101 68-69 0.650463677325817 1101 70-71 0.5639063525631229 1101 72-73 0.6340863130332437 1101 74-75 0.7100268553863458 1101 76-77 0.5178995810886455 1101 78-79 0.5763405864597395 1101 80-81 0.6671071607416259 1101 82-83 0.6559943507407056 1101 84-85 0.6863718979357856 1101 86-87 0.6955639883349214 1101 88-89 0.7521442987070444 1101 90-91 0.7165461390017605 1101 92-93 0.6093561965942413 1101 94-95 0.7995473967214046 1101 96-97 0.9751209294691883 1101 98-99 0.9645720629150496 1101 100-101 1.2568245747702314 1102 1 0.2865303679425004 1102 2 0.36354004290492625 1102 3 0.3904742818903095 1102 4 0.2203605527339434 1102 5 0.34512905605759414 1102 6 0.28056887226887284 1102 7 0.2931378307674919 1102 8 0.3742951168379207 1102 9 0.5509898704626721 1102 10-11 0.4623701810574019 1102 12-13 0.4073976019150294 1102 14-15 0.4801525813497136 1102 16-17 0.4956590604973883 1102 18-19 0.4903557925252855 1102 20-21 0.5720187034600954 1102 22-23 0.5496452956087339 1102 24-25 0.5714390745078006 1102 26-27 0.5728674912572984 1102 28-29 0.49223576250768986 1102 30-31 0.4346868053452866 1102 32-33 0.6800473588068243 1102 34-35 0.6138375694086804 1102 36-37 0.6374509177278327 1102 38-39 0.6199472302511992 1102 40-41 0.6895650108865894 1102 42-43 0.6111251441721208 1102 44-45 0.6899990245152168 1102 46-47 0.6227319202799535 1102 48-49 0.6033917745384656 1102 50-51 0.43631575484657503 1102 52-53 0.338462406699108 1102 54-55 0.6024822816699498 1102 56-57 0.525123691706213 1102 58-59 0.5578619050156703 1102 60-61 0.4262511263386983 1102 62-63 0.22543420802495007 1102 64-65 0.32383229765228805 1102 66-67 0.19009435894675875 1102 68-69 0.39569290038541993 1102 70-71 0.43765900398382485 1102 72-73 0.4756985659766997 1102 74-75 0.5185777295609881 1102 76-77 0.5589365579410739 1102 78-79 0.61671244796932 1102 80-81 0.5201265729855074 1102 82-83 0.4371854229400185 1102 84-85 0.4580308741889141 1102 86-87 0.48801762470492704 1102 88-89 0.6010364351910482 1102 90-91 0.44397881158152686 1102 92-93 0.5713391382633759 1102 94-95 0.5909252208467741 1102 96-97 0.6339872480170996 1102 98-99 0.6726297669980852 1102 100-101 0.8400706447480459 1103 1 0.36330844860430744 1103 2 0.4466794434275876 1103 3 0.3739053530446057 1103 4 0.25124145328131675 1103 5 0.26748213482592575 1103 6 0.3379503272644797 1103 7 0.40525721267692205 1103 8 0.41781849461558096 1103 9 0.43092361287285996 1103 10-11 0.46998151791098053 1103 12-13 0.516706171835601 1103 14-15 0.5171867353182265 1103 16-17 0.5324534822237368 1103 18-19 0.45371786567620376 1103 20-21 0.49657474027830517 1103 22-23 0.4295879389685098 1103 24-25 0.5169903475377922 1103 26-27 0.5861164737978442 1103 28-29 0.4761738641174844 1103 30-31 0.5762427603826481 1103 32-33 0.6404840404723942 1103 34-35 0.6324694146974466 1103 36-37 0.6821404277915164 1103 38-39 0.5470004959071133 1103 40-41 0.5356142407980116 1103 42-43 0.7397878849266277 1103 44-45 0.7433427929981704 1103 46-47 0.8369518831354767 1103 48-49 0.6864774456927307 1103 50-51 0.583645836797885 1103 52-53 0.58541245586472 1103 54-55 0.600095640387714 1103 56-57 0.6252716200938124 1103 58-59 0.7159816101644338 1103 60-61 0.50476446060852 1103 62-63 0.4700531254666416 1103 64-65 0.41775765310227087 1103 66-67 0.42846502555990895 1103 68-69 0.41013013272307575 1103 70-71 0.45428948424454063 1103 72-73 0.5067051047760955 1103 74-75 0.5558874892957633 1103 76-77 0.49746196081963134 1103 78-79 0.5148900082114842 1103 80-81 0.514867514354254 1103 82-83 0.4485875393592593 1103 84-85 0.3615249985911717 1103 86-87 0.3690781194423671 1103 88-89 0.4194536650990166 1103 90-91 0.40352275497623324 1103 92-93 0.3361308981202882 1103 94-95 0.41429132477922437 1103 96-97 0.6792083875242199 1103 98-99 0.6462373725735979 1103 100-101 0.8893314127931831 1104 1 0.29025029000448654 1104 2 0.39833814822237557 1104 3 0.38308531911986776 1104 4 0.24852880715167203 1104 5 0.3031530981818804 1104 6 0.3554733880890737 1104 7 0.2632065321826431 1104 8 0.24312962412943762 1104 9 0.43498154319718196 1104 10-11 0.48935036113736174 1104 12-13 0.5025744393744915 1104 14-15 0.6238109738444777 1104 16-17 0.6399004289972225 1104 18-19 0.6076052350426338 1104 20-21 0.5928912775888548 1104 22-23 0.5465011334592376 1104 24-25 0.6021178444052495 1104 26-27 0.6146491413554003 1104 28-29 0.6251549764023707 1104 30-31 0.6146333472673078 1104 32-33 0.6297278070515304 1104 34-35 0.5045402609164 1104 36-37 0.5060094226461516 1104 38-39 0.6034561937969727 1104 40-41 0.4138652921983237 1104 42-43 0.5554465638946198 1104 44-45 0.6048077843670256 1104 46-47 0.4088801196488916 1104 48-49 0.40992427666220976 1104 50-51 0.43812509788507725 1104 52-53 0.33413527941608123 1104 54-55 0.45771587659454127 1104 56-57 0.5137413327665996 1104 58-59 0.4956690893363742 1104 60-61 0.433088347723789 1104 62-63 0.3692709486181158 1104 64-65 0.31366319036617085 1104 66-67 0.33350376991133857 1104 68-69 0.32816412661129135 1104 70-71 0.30521599952733425 1104 72-73 0.28061092363532225 1104 74-75 0.318331107745756 1104 76-77 0.4578602224266888 1104 78-79 0.5341818917676484 1104 80-81 0.4658376435881806 1104 82-83 0.3565827222462623 1104 84-85 0.33233291475241344 1104 86-87 0.3670126010403614 1104 88-89 0.5406164080374722 1104 90-91 0.5324260369781264 1104 92-93 0.4929578352962025 1104 94-95 0.6302020440636724 1104 96-97 0.7378312447337265 1104 98-99 0.8155217788573417 1104 100-101 0.9632683071132888 1105 1 0.26058575243373383 1105 2 0.34316584074976575 1105 3 0.29724559200764844 1105 4 0.018917496978744452 1105 5 0.1458985933264998 1105 6 0.20375258526412665 1105 7 0.23765089007847706 1105 8 0.2557784336532478 1105 9 0.36760912667332235 1105 10-11 0.2947474245428836 1105 12-13 0.19467706763953885 1105 14-15 0.3677628484874589 1105 16-17 0.398183131391157 1105 18-19 0.42314674267621655 1105 20-21 0.3899021859598335 1105 22-23 0.4008264275081501 1105 24-25 0.37152109711855275 1105 26-27 0.3343724075702781 1105 28-29 0.371877353330234 1105 30-31 0.3676610612154789 1105 32-33 0.30328239109888955 1105 34-35 0.46241475136142896 1105 36-37 0.4445001338161134 1105 38-39 0.41241851918514527 1105 40-41 0.31332474200878124 1105 42-43 0.37476510715161737 1105 44-45 0.48198028981958885 1105 46-47 0.4431622962599917 1105 48-49 0.40059528266003497 1105 50-51 0.3141064780325564 1105 52-53 0.33697515582466053 1105 54-55 0.3813897354510587 1105 56-57 0.43860875110798503 1105 58-59 0.6397368309368332 1105 60-61 0.4236676662832721 1105 62-63 0.25806057706491714 1105 64-65 0.2830347176030159 1105 66-67 0.2614524541560286 1105 68-69 0.15044750083884395 1105 70-71 0.11861989161852193 1105 72-73 0.25113219512100926 1105 74-75 0.35195078176040084 1105 76-77 0.3887664351025997 1105 78-79 0.5743251675894321 1105 80-81 0.525651120505664 1105 82-83 0.5228120479460081 1105 84-85 0.49665668338199964 1105 86-87 0.4284569784096277 1105 88-89 0.4525335842591076 1105 90-91 0.5759349720407023 1105 92-93 0.6741808970399816 1105 94-95 0.6509234051271804 1105 96-97 0.6709343046599869 1105 98-99 0.7231097663424073 1105 100-101 0.7982528257298966 1106 1 0.24318052707554472 1106 2 0.2088859707586792 1106 3 0.18571123279754076 1106 4 0.0058560169579351395 1106 5 0.09603126371116133 1106 6 0.114300888978498 1106 7 0.349435064800133 1106 8 0.17508641040971895 1106 9 0.3560766829170916 1106 10-11 0.3040967549405238 1106 12-13 0.2519976803428321 1106 14-15 0.3221948559234491 1106 16-17 0.22121086917962884 1106 18-19 0.23108730715039627 1106 20-21 0.1761418173055347 1106 22-23 0.216072281054565 1106 24-25 0.31628475027219594 1106 26-27 0.35172873844780383 1106 28-29 0.4043232770427494 1106 30-31 0.4046834075053525 1106 32-33 0.44979344225965434 1106 34-35 0.4415486032787044 1106 36-37 0.3444278506354195 1106 38-39 0.3280074406031446 1106 40-41 0.2525186081205746 1106 42-43 0.3399280417076156 1106 44-45 0.4717363416457161 1106 46-47 0.25923194619567624 1106 48-49 0.2902223437892246 1106 50-51 0.283890697112092 1106 52-53 0.2130434750239587 1106 54-55 0.29834906330821553 1106 56-57 0.3978887219124374 1106 58-59 0.43828123720661694 1106 60-61 0.3812359774174965 1106 62-63 0.19439920713229952 1106 64-65 0.2444229982512809 1106 66-67 0.122863619437517 1106 68-69 0.2714454119859724 1106 70-71 0.2587419463561389 1106 72-73 0.2570395557062142 1106 74-75 0.41257852640418236 1106 76-77 0.3653924538216238 1106 78-79 0.4144314450358699 1106 80-81 0.3189881133640462 1106 82-83 0.39049219134293267 1106 84-85 0.35376891486561135 1106 86-87 0.3976560767349149 1106 88-89 0.48292165521558417 1106 90-91 0.47852547219595465 1106 92-93 0.4490608787322756 1106 94-95 0.5373261020862472 1106 96-97 0.5516127180996193 1106 98-99 0.6355762963061586 1106 100-101 0.8433449792242875 1107 1 0.1658601946638072 1107 2 0.14705392223226 1107 3 0.12380300699614821 1107 4 -0.030382757474178845 1107 5 0.12222379903526104 1107 6 0.09237726839708671 1107 7 0.10559947427937288 1107 8 0.14165653607235384 1107 9 0.17805069003593132 1107 10-11 0.18165766813179118 1107 12-13 0.10682440691569894 1107 14-15 0.12556955899389521 1107 16-17 0.22313237870453406 1107 18-19 0.23344710984627426 1107 20-21 0.2047239789776114 1107 22-23 0.18426189575190222 1107 24-25 0.18473172466517696 1107 26-27 0.20625170191307518 1107 28-29 0.39462058250814636 1107 30-31 0.3763125506404563 1107 32-33 0.36680029273760084 1107 34-35 0.24015515661549358 1107 36-37 0.34212689861863765 1107 38-39 0.3613985790451366 1107 40-41 0.345016092527878 1107 42-43 0.18270710819245295 1107 44-45 0.1338105642018519 1107 46-47 0.2056445193295957 1107 48-49 0.1602177582160067 1107 50-51 0.11938163973367466 1107 52-53 0.08977629642608775 1107 54-55 0.10091564000602915 1107 56-57 0.14026539314475883 1107 58-59 0.05300894327983485 1107 60-61 0.0889917700022167 1107 62-63 -0.1039396303825697 1107 64-65 -0.12601275069829398 1107 66-67 -0.04198236056818416 1107 68-69 0.050661363606941734 1107 70-71 -0.02456069201639366 1107 72-73 -0.02986711418678567 1107 74-75 0.1405276200688803 1107 76-77 0.16637613645017169 1107 78-79 0.22964967301304284 1107 80-81 0.30329514661766765 1107 82-83 0.3596213257649552 1107 84-85 0.22578228024318747 1107 86-87 0.28134313650619447 1107 88-89 0.4449392676007129 1107 90-91 0.3494119117945935 1107 92-93 0.252754779521581 1107 94-95 0.4343158549980366 1107 96-97 0.5235197838157077 1107 98-99 0.6258014574111357 1107 100-101 0.8726498790163184 1108 1 0.09798549869400475 1108 2 0.17568048308531203 1108 3 0.1411432585055259 1108 4 0.011244980509829361 1108 5 0.08567364299276647 1108 6 0.1178618454963285 1108 7 0.10955422728684994 1108 8 0.10444966795201793 1108 9 0.13455271197923935 1108 10-11 0.2508587522546364 1108 12-13 0.09202475644463703 1108 14-15 0.09073529807432124 1108 16-17 0.21007176286867235 1108 18-19 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-1.9813878741688207 2316 78-79 -1.5504331925130295 2316 80-81 -1.1621556478571087 2316 82-83 -0.8490365318281228 2316 84-85 -0.9944540121474752 2316 86-87 -0.8511536118509824 2316 88-89 -0.8600693585905326 2316 90-91 -0.9311669548048798 2316 92-93 -0.9928404539317341 2316 94-95 -1.456244554007231 2316 96-97 -1.2816674579023655 2316 98-99 -1.2422915339939173 2316 100-101 -1.639826950525915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 23.0 5 61.0 6 123.0 7 359.0 8 881.0 9 1729.0 10 3083.0 11 5428.0 12 7576.0 13 9990.0 14 12237.0 15 15820.0 16 19069.0 17 22336.0 18 19119.0 19 14698.0 20 13980.0 21 14464.0 22 16219.0 23 18828.0 24 23303.0 25 29671.0 26 38313.0 27 49930.0 28 63182.0 29 79796.0 30 98092.0 31 119661.0 32 144844.0 33 176284.0 34 217640.0 35 275148.0 36 360479.0 37 505511.0 38 519422.0 39 109525.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.87846609592538 22.282490663454322 15.220739586787388 22.618303653832914 2 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19.877380594073895 20-21 20.644990712918602 28.45252447316391 31.05591704316622 19.846567770751264 22-23 20.374151543314422 28.229232090058172 30.962585011955174 20.43403135467223 24-25 20.222515515840268 28.50852213440227 31.014153865187332 20.25480848457013 26-27 20.283891501962646 28.63818716518405 30.836882947137013 20.241038385716294 28-29 20.183493866354844 28.692375895369853 30.92330931490612 20.20082092336918 30-31 20.560244576306868 28.447527812339835 30.83623438429704 20.155993227056253 32-33 20.356205995666343 28.67025165672179 30.692912537197653 20.28062981041421 34-35 20.449279144029997 28.588567852463054 30.821858461137275 20.140294542369674 36-37 20.48180910552428 28.427758233410955 30.847446184258924 20.242986476805836 38-39 20.280419381921998 28.724794872779803 30.892808711831155 20.101977033467044 40-41 20.467382219900088 28.63033293412855 30.78158221451232 20.120702631459046 42-43 20.404863295483384 28.661881955921807 30.506538953162142 20.426715795432663 44-45 20.451906597366694 28.905764875663493 30.32600521712118 20.316323309848638 46-47 20.508426686472315 28.7729172594697 30.181009825382887 20.537646228675097 48-49 20.58293460620017 28.910811790205145 30.014503351270307 20.491750252324387 50-51 20.62639913460864 29.077576177813736 29.700729928223364 20.595294759354264 52-53 20.646627356056243 29.190492203913525 29.62452928818051 20.538351151849717 54-55 20.60416481988033 29.127993644116774 29.728489561339945 20.53935197466295 56-57 20.631699486507816 29.254702965110535 29.559176323816374 20.554421224565274 58-59 20.529663976795707 29.230925311175714 29.740248940572258 20.499161771456322 60-61 20.643177792506677 29.310312964665798 29.670549401773094 20.375959841054435 62-63 20.53895967411663 29.21627610011389 29.711655090877542 20.533109134891934 64-65 20.395652879091635 29.103830126434026 29.74394313010775 20.756573864366587 66-67 20.43576284754719 29.151953359301885 29.62425177601609 20.788032017134842 68-69 20.47344245985771 29.24443379043693 29.554279815949425 20.727843933755928 70-71 20.539161874905616 28.986767972312517 29.676488207250763 20.797581945531103 72-73 20.556729489481146 29.148276932040133 29.512432401642297 20.78256117683642 74-75 20.46157852860694 29.309464595717376 29.41033767304318 20.818619202632505 76-77 20.399551758182703 29.232365058800497 29.534686668138587 20.833396514878213 78-79 20.31681720059607 29.25930255781572 29.488874214188943 20.935006027399265 80-81 20.41356044037908 29.238435792240686 29.41258733351425 20.93541643386598 82-83 20.530120241514002 29.271467126059868 29.483914309421937 20.71449832300419 84-85 20.55037389440497 29.164378846406475 29.377175230680457 20.908072028508098 86-87 20.378882989425865 29.26574688255043 29.43389543594408 20.921474692079624 88-89 20.481927510498483 29.208297817359817 29.333128912092103 20.976645760049593 90-91 20.45144933272225 29.155427855312183 29.499260649736886 20.89386216222868 92-93 20.46241067187523 29.223036577463834 29.340156043976513 20.974396706684427 94-95 20.21067671238435 29.150967461280096 29.398623846650928 21.239731979684624 96-97 20.261754395650353 29.1259217034888 29.385976654418585 21.226347246442266 98-99 20.216537822615912 29.172576753529007 29.27833474399416 21.33255067986092 100-101 20.178890638588694 29.12151027731541 29.2614400713035 21.438159012792394 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 311.0 1 215.0 2 191.0 3 284.0 4 248.5 5 241.5 6 424.5 7 921.0 8 1561.0 9 1949.5 10 1849.5 11 1651.5 12 1806.0 13 2105.0 14 2436.0 15 2699.5 16 3027.0 17 3481.5 18 3957.0 19 4524.0 20 5257.0 21 6314.5 22 7640.5 23 9692.5 24 12379.0 25 16082.0 26 21652.5 27 28291.5 28 35527.5 29 44676.0 30 54774.0 31 65182.0 32 77231.5 33 88346.0 34 97443.5 35 104168.0 36 113286.0 37 123074.5 38 124867.5 39 123836.0 40 122778.5 41 120741.0 42 119803.0 43 120378.5 44 121013.0 45 121438.5 46 119342.5 47 115505.5 48 112835.5 49 107979.0 50 100692.5 51 92325.0 52 83032.0 53 73732.5 54 64365.0 55 53978.5 56 43561.5 57 33995.0 58 26004.5 59 19775.5 60 14129.5 61 9746.5 62 6610.5 63 4109.5 64 2448.0 65 1372.5 66 752.0 67 421.5 68 236.0 69 134.0 70 69.0 71 35.5 72 23.5 73 14.0 74 10.0 75 9.5 76 8.5 77 3.5 78 1.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004389954321194998 2 0.006717960400616589 3 0.0016961187150071587 4 0.17370251075455664 5 0.00232800607942159 6 4.3234398617829535E-4 7 9.977168911806816E-5 8 8.31430742650568E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.826864525011931E-4 24-25 6.152587495614202E-4 26-27 0.002743721450746874 28-29 2.3280060794215904E-4 30-31 0.006518417022380453 32-33 0.020852283025676245 34-35 0.01345254941608619 36-37 0.0010309741208867043 38-39 3.824581416192613E-4 40-41 0.0017958904041252268 42-43 0.011440487018871815 44-45 0.007516133913561134 46-47 0.010492655972250168 48-49 0.010592427661368236 50-51 0.18888443611535602 52-53 0.11423858404018804 54-55 0.017177359143160733 56-57 0.006584931481792499 58-59 0.017460045595661926 60-61 0.12300186406772502 62-63 0.23750650594556122 64-65 0.24332652114411524 66-67 0.25262191684694857 68-69 0.24849802036340177 70-71 0.23605981645334925 72-73 0.1613141926890632 74-75 0.26762092744436483 76-77 0.20794082873690703 78-79 0.005936415502525055 80-81 3.325722970602272E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 6.651445941204544E-5 90-91 0.005686986279729885 92-93 0.01772610343331011 94-95 0.002594063917069772 96-97 1.662861485301136E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3006865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.32657013207221 #Duplication Level Percentage of deduplicated Percentage of total 1 72.00435273721514 39.11749518792785 2 15.868435811405138 17.24155381989175 3 5.4855548893627235 8.94034147230887 4 2.3879001600959016 5.189057020633459 5 1.2567362107375943 3.413708394507531 6 0.7572691318503832 2.4683900760193977 7 0.4428657510827346 1.6841564099702313 8 0.32521064960866775 1.413406333092964 9 0.2317122364887911 1.1329317959460854 >10 1.1103044238086743 10.870935274453613 >50 0.08559938514656182 3.214862735747992 >100 0.0424657794023402 4.060360480818972 >500 0.0011027310892324142 0.3827762682673678 >1k 3.675770295543315E-4 0.3631731708150699 >5k 1.2252567651811048E-4 0.5068515595988544 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8107 0.26961636122672616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7122 0.23685798996629379 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4240 0.14101065395353632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.325722970602272E-5 0.0 3 0.0 0.0 0.0 3.325722970602272E-5 0.0 4 0.0 0.0 0.0 6.651445941204544E-5 0.0 5 0.0 0.0 0.0 6.651445941204544E-5 0.0 6 0.0 0.0 0.0 6.651445941204544E-5 0.0 7 0.0 0.0 0.0 9.977168911806816E-5 0.0 8 0.0 0.0 0.0 9.977168911806816E-5 3.325722970602272E-5 9 0.0 0.0 0.0 1.3302891882409088E-4 6.651445941204544E-5 10-11 0.0 0.0 0.0 1.6628614853011358E-4 6.651445941204544E-5 12-13 0.0 0.0 0.0 1.995433782361363E-4 6.651445941204544E-5 14-15 0.0 4.988584455903408E-5 0.0 1.995433782361363E-4 6.651445941204544E-5 16-17 0.0 9.977168911806816E-5 0.0 1.995433782361363E-4 6.651445941204544E-5 18-19 1.662861485301136E-5 9.977168911806816E-5 0.0 2.1617199308914767E-4 6.651445941204544E-5 20-21 3.325722970602272E-5 9.977168911806816E-5 0.0 2.6605783764818176E-4 1.3302891882409088E-4 22-23 3.325722970602272E-5 9.977168911806816E-5 0.0 3.4920091191323853E-4 1.3302891882409088E-4 24-25 3.325722970602272E-5 9.977168911806816E-5 0.0 3.990867564722726E-4 1.3302891882409088E-4 26-27 3.325722970602272E-5 9.977168911806816E-5 0.0 5.986301347084089E-4 1.3302891882409088E-4 28-29 3.325722970602272E-5 9.977168911806816E-5 0.0 9.312024317686362E-4 1.3302891882409088E-4 30-31 3.325722970602272E-5 9.977168911806816E-5 0.0 0.0027270928358938628 1.3302891882409088E-4 32-33 3.325722970602272E-5 9.977168911806816E-5 0.0 0.007848706210621361 1.3302891882409088E-4 34-35 3.325722970602272E-5 1.1640030397107952E-4 0.0 0.015281697049917439 1.3302891882409088E-4 36-37 3.325722970602272E-5 1.6628614853011358E-4 0.0 0.02876750369570965 1.3302891882409088E-4 38-39 3.325722970602272E-5 1.995433782361363E-4 0.0 0.04837264060741005 1.3302891882409088E-4 40-41 3.325722970602272E-5 1.995433782361363E-4 0.0 0.0713035004897127 1.3302891882409088E-4 42-43 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.09681179567423213 1.3302891882409088E-4 44-45 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.12602827197097308 1.3302891882409088E-4 46-47 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.16269436772186313 1.3302891882409088E-4 48-49 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.2037670464088012 1.3302891882409088E-4 50-51 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.24952899448428845 1.3302891882409088E-4 52-53 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.29590620130933715 1.3302891882409088E-4 54-55 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.3474881645833784 1.3302891882409088E-4 56-57 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.4103775859574673 1.4965753367710222E-4 58-59 3.325722970602272E-5 2.6605783764818176E-4 0.0 0.4814482858392378 1.6628614853011358E-4 60-61 3.325722970602272E-5 2.9931506735420444E-4 0.0 0.5625959263219333 1.6628614853011358E-4 62-63 3.325722970602272E-5 2.9931506735420444E-4 0.0 0.6444752258581612 1.6628614853011358E-4 64-65 3.325722970602272E-5 2.9931506735420444E-4 0.0 0.7329394568761817 1.6628614853011358E-4 66-67 3.325722970602272E-5 3.3257229706022717E-4 0.0 0.8353052099113196 1.6628614853011358E-4 68-69 3.325722970602272E-5 3.3257229706022717E-4 0.0 0.9554469522243266 1.8291476338312495E-4 70-71 3.325722970602272E-5 3.3257229706022717E-4 0.0 1.0896232454732753 1.995433782361363E-4 72-73 3.325722970602272E-5 3.3257229706022717E-4 0.0 1.2292703530088647 1.995433782361363E-4 74-75 3.325722970602272E-5 3.3257229706022717E-4 0.0 1.3726921561160879 1.995433782361363E-4 76-77 3.325722970602272E-5 3.3257229706022717E-4 0.0 1.5345717217101533 1.995433782361363E-4 78-79 3.325722970602272E-5 3.3257229706022717E-4 0.0 1.7295588594765645 1.995433782361363E-4 80-81 3.325722970602272E-5 3.3257229706022717E-4 0.0 1.9445834781408542 2.1617199308914767E-4 82-83 3.325722970602272E-5 3.3257229706022717E-4 0.0 2.1720463007151967 2.3280060794215904E-4 84-85 3.325722970602272E-5 3.3257229706022717E-4 0.0 2.3974970608923245 2.3280060794215904E-4 86-87 3.325722970602272E-5 3.3257229706022717E-4 0.0 2.6452966794319 2.3280060794215904E-4 88-89 3.325722970602272E-5 3.3257229706022717E-4 0.0 2.926569699670587 2.3280060794215904E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4210 0.0 59.033287 1 GTATCAA 8010 0.0 42.714695 1 ATCAACG 9755 0.0 34.5362 3 TCAACGC 9920 0.0 34.153362 4 CAACGCA 9865 0.0 34.14013 5 TATCAAC 10400 0.0 32.532448 2 AACGCAG 10530 0.0 32.253834 6 ACGCAGA 11890 0.0 28.165634 7 CGCAGAG 11920 0.0 28.01516 8 GCAGAGT 13835 0.0 23.72595 9 GTACATG 8015 0.0 22.885536 1 GGACCGT 270 4.1836756E-11 22.838959 6 GCCGCGA 125 2.2781828E-4 22.768682 8 ACATGGG 7470 0.0 22.454828 3 GTGGTAT 2055 0.0 22.430412 1 TACATGG 7975 0.0 22.404087 2 CAGAGTA 13325 0.0 20.967407 10-11 GAGTACT 9960 0.0 20.955114 12-13 AGAGTAC 12615 0.0 20.793833 10-11 TAACGCA 260 1.4861143E-8 20.103653 4 >>END_MODULE