FastQCFastQC Report
Sat 18 Jun 2016
SRR1930982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4979064
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2275074.569272457634608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31900.054.258021
GTATCAA64950.048.473691
ATCAACG78000.039.1635673
TCAACGC80150.038.172514
CAACGCA81700.037.1319475
TATCAAC83550.036.632832
AACGCAG89250.034.3127756
ACGCAGA99150.029.42727
CGCAGAG101950.028.4502358
GTACATG73850.025.036681
GCAGAGT116150.024.4089789
ACATGGG72600.023.994073
TACATGG75450.023.617882
AGAGTAC101250.021.78450810-11
GAGTACT77350.020.61697612-13
CAGAGTA111850.019.781310-11
AGTACTT84350.018.79213112-13
GTACTTT86950.018.42442514-15
CAACGCG2803.7774407E-718.0275735
GAGTACA56350.017.499761
Produced by FastQC (version 0.11.5)