FastQCFastQC Report
Sat 18 Jun 2016
SRR1930982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4979064
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109340.21959950705594464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87380.1754948319603845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58070.11662834621125578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59000.053.808311
GTATCAA115450.044.21151
ATCAACG142950.035.405323
CAACGCA144300.035.0757265
TCAACGC145150.034.8676384
TATCAAC151850.033.5196532
AACGCAG152650.033.343526
ACGCAGA172000.029.178697
CGCAGAG172300.029.1003578
GCAGAGT204350.024.0717899
GTACATG116600.023.5180971
ACATGGG111250.022.170243
AGAGTAC187400.021.401710-11
TACATGG121950.021.3951572
CAGAGTA197900.019.85870410-11
GAGTACT151450.019.82616212-13
ACGCGTC1200.00480296719.7650248
AGTACTT161800.019.15893212-13
GTGGTAT30950.018.5801661
GTACTTT163900.018.11755614-15
Produced by FastQC (version 0.11.5)