##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930982_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4979064 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3456587422857 31.0 31.0 34.0 30.0 34.0 2 31.500249846155825 31.0 31.0 34.0 30.0 34.0 3 31.570432916708842 31.0 31.0 34.0 30.0 34.0 4 35.34213940612131 37.0 35.0 37.0 33.0 37.0 5 35.21642280557149 37.0 35.0 37.0 33.0 37.0 6 35.23399518463711 37.0 35.0 37.0 32.0 37.0 7 35.11334560069925 37.0 35.0 37.0 32.0 37.0 8 35.11717684287649 37.0 35.0 37.0 32.0 37.0 9 36.621284040534526 39.0 35.0 39.0 32.0 39.0 10-11 36.56375947366814 38.5 35.0 39.0 32.0 39.0 12-13 36.448441815570156 38.0 35.0 39.0 32.0 39.0 14-15 37.51482798775031 39.0 36.0 41.0 32.0 41.0 16-17 37.47206161640019 39.0 36.0 41.0 32.0 41.0 18-19 37.49997298689071 39.0 36.0 41.0 32.0 41.0 20-21 37.483870763661606 39.0 36.0 41.0 32.0 41.0 22-23 37.38138372995407 39.0 36.0 41.0 32.0 41.0 24-25 37.254997425218875 39.0 36.0 41.0 31.0 41.0 26-27 37.00537671337424 39.0 36.0 40.0 31.0 41.0 28-29 36.81837650610637 39.0 36.0 40.0 30.5 41.0 30-31 36.59673143385986 38.5 35.0 40.0 30.0 41.0 32-33 36.43455245805236 38.0 35.0 40.0 30.0 41.0 34-35 36.50970855164746 38.0 35.0 40.0 30.0 41.0 36-37 36.57140257686987 39.0 35.0 40.0 30.0 41.0 38-39 36.558482678672135 39.0 35.0 40.0 30.0 41.0 40-41 36.45089890389037 39.0 35.0 40.0 30.0 41.0 42-43 36.315603394533596 39.0 35.0 40.0 30.0 41.0 44-45 36.155881306205345 38.5 35.0 40.0 30.0 41.0 46-47 35.925898120610626 38.0 35.0 40.0 29.0 41.0 48-49 35.721763066311254 38.0 35.0 40.0 28.0 41.0 50-51 35.467251374957215 38.0 34.5 40.0 27.5 41.0 52-53 35.26593542481076 38.0 34.0 40.0 27.0 41.0 54-55 35.11936450304716 38.0 34.0 40.0 26.0 41.0 56-57 34.951858522003334 38.0 34.0 40.0 26.0 41.0 58-59 34.75706769384768 38.0 33.5 40.0 26.0 41.0 60-61 34.4583910751097 38.0 33.0 40.0 26.0 41.0 62-63 34.209606765448285 37.0 33.0 40.0 25.5 41.0 64-65 33.90331636628892 37.0 32.5 40.0 25.0 41.0 66-67 33.58453285597454 36.0 32.0 39.0 23.5 41.0 68-69 33.24555328069694 36.0 32.0 39.0 23.0 40.0 70-71 32.873282910201596 35.5 31.5 39.0 22.5 40.0 72-73 32.45203526204925 35.0 31.0 38.0 22.0 40.0 74-75 31.936366855296498 35.0 31.0 37.5 21.0 39.5 76-77 30.529058172379386 33.5 29.5 36.0 20.0 39.0 78-79 31.268739967993987 34.0 30.0 36.0 20.0 39.0 80-81 31.232591908840696 34.0 31.0 36.0 20.0 38.5 82-83 30.96164098312454 34.0 30.5 36.0 20.0 37.0 84-85 30.621374017285177 34.0 30.0 35.0 19.5 37.0 86-87 30.277629992303773 34.0 30.0 35.0 18.0 36.5 88-89 29.92562979708636 34.0 30.0 35.0 16.5 36.0 90-91 29.592028140228766 34.0 29.0 35.0 10.0 36.0 92-93 29.276169275992437 34.0 29.0 35.0 6.5 35.5 94-95 28.89852309590718 34.0 29.0 35.0 2.0 35.0 96-97 28.481677781205462 34.0 29.0 35.0 2.0 35.0 98-99 27.993438425374727 34.0 29.0 35.0 2.0 35.0 100-101 26.86224449012907 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31280646786946775 1101 2 0.37349060381111343 1101 3 0.2653630493359884 1101 4 0.18012256946476413 1101 5 0.31112457390032944 1101 6 0.29719842133143004 1101 7 0.38472845777945963 1101 8 0.3975873289743461 1101 9 0.4646468637992669 1101 10-11 0.4201784506582058 1101 12-13 0.4115674309184669 1101 14-15 0.47343582749027036 1101 16-17 0.6279884982574444 1101 18-19 0.5133481135007187 1101 20-21 0.5416703697356269 1101 22-23 0.5211605737545071 1101 24-25 0.5348273493704525 1101 26-27 0.5816003071417697 1101 28-29 0.5622967053170029 1101 30-31 0.49377670385778316 1101 32-33 0.5209329176060891 1101 34-35 0.5726788349880678 1101 36-37 0.5667819493000934 1101 38-39 0.5193806748877634 1101 40-41 0.5128801168819948 1101 42-43 0.5080682554862506 1101 44-45 0.48067480164909426 1101 46-47 0.4558790440749192 1101 48-49 0.49456509835448514 1101 50-51 0.46583550923232764 1101 52-53 0.45588916401296586 1101 54-55 0.5063158268958787 1101 56-57 0.5692235939459565 1101 58-59 0.49555485986627446 1101 60-61 0.38937211067847954 1101 62-63 0.1901179883599582 1101 64-65 0.32723142270391037 1101 66-67 0.3248428975110258 1101 68-69 0.38146844519214085 1101 70-71 0.2428152161306727 1101 72-73 0.2720354761881225 1101 74-75 0.2670192935359701 1101 76-77 0.18083158804114063 1101 78-79 0.23700403643487533 1101 80-81 0.18340032925160799 1101 82-83 0.1837117064384408 1101 84-85 0.22704869073525558 1101 86-87 0.15250564310953152 1101 88-89 0.1921327170631102 1101 90-91 0.2761901078516331 1101 92-93 0.26580348692415257 1101 94-95 0.31888523588801476 1101 96-97 0.4532163361730035 1101 98-99 0.5379195539557422 1101 100-101 0.6846147120689494 1102 1 0.3308143726804502 1102 2 0.41398914443908197 1102 3 0.40962540662545166 1102 4 0.15464490992560798 1102 5 0.24614715009721522 1102 6 0.3552813314517067 1102 7 0.4512304717676514 1102 8 0.3880628196775149 1102 9 0.4957628120913782 1102 10-11 0.4757345701755753 1102 12-13 0.5080397047834708 1102 14-15 0.5883280406923248 1102 16-17 0.6222786870621917 1102 18-19 0.6296521043845189 1102 20-21 0.5514466275557623 1102 22-23 0.4856178475244448 1102 24-25 0.5295570054149792 1102 26-27 0.5039421819184042 1102 28-29 0.4456481740508025 1102 30-31 0.4998800798298575 1102 32-33 0.6962322483928887 1102 34-35 0.5283109725290132 1102 36-37 0.5738619459936558 1102 38-39 0.6352884826006289 1102 40-41 0.7994116292954416 1102 42-43 0.7256319718914455 1102 44-45 0.7161931083328241 1102 46-47 0.7116142738345701 1102 48-49 0.716702947861755 1102 50-51 0.5714193515009143 1102 52-53 0.5278032576441589 1102 54-55 0.7210618289706119 1102 56-57 0.7057049097300876 1102 58-59 0.6811383565068283 1102 60-61 0.5275724021983947 1102 62-63 0.4314209721691 1102 64-65 0.38101605761274726 1102 66-67 0.3371675376147749 1102 68-69 0.4494455415751517 1102 70-71 0.4567137044552183 1102 72-73 0.40390946568940933 1102 74-75 0.5238998491640352 1102 76-77 0.5377557258321595 1102 78-79 0.5724624181480742 1102 80-81 0.6072771066578149 1102 82-83 0.6256565714372613 1102 84-85 0.6259958989148267 1102 86-87 0.562884478009547 1102 88-89 0.5617401343855022 1102 90-91 0.5496976070935062 1102 92-93 0.5220899893522493 1102 94-95 0.6406136450935449 1102 96-97 0.7424991564138814 1102 98-99 0.8258285430880399 1102 100-101 1.0960991538128226 1103 1 0.3518744643847711 1103 2 0.38887233722549297 1103 3 0.36299637377454985 1103 4 0.2423210664089126 1103 5 0.321044010389258 1103 6 0.3440632712169105 1103 7 0.3713380132688684 1103 8 0.3228866605178027 1103 9 0.44612157519282647 1103 10-11 0.40893740202565 1103 12-13 0.4595311222679612 1103 14-15 0.5775376698961097 1103 16-17 0.567010042068695 1103 18-19 0.6028718225626974 1103 20-21 0.5694413439764361 1103 22-23 0.47342414863708626 1103 24-25 0.5985789794096021 1103 26-27 0.6403517388949567 1103 28-29 0.5590513579729688 1103 30-31 0.5643982003372869 1103 32-33 0.690658546814845 1103 34-35 0.6577078144430715 1103 36-37 0.6113608709711897 1103 38-39 0.6669416048762784 1103 40-41 0.6483865842158707 1103 42-43 0.6357931021477725 1103 44-45 0.7564326715150571 1103 46-47 0.6825460230219207 1103 48-49 0.6797988983903807 1103 50-51 0.7032049736418458 1103 52-53 0.7232362766962339 1103 54-55 0.6982185381635659 1103 56-57 0.7320021897537217 1103 58-59 0.7030391960770714 1103 60-61 0.5571989509890685 1103 62-63 0.48690087469087473 1103 64-65 0.5611163121566634 1103 66-67 0.48027720560858 1103 68-69 0.39272575006349086 1103 70-71 0.5333013386813619 1103 72-73 0.587732777315324 1103 74-75 0.6062704797998713 1103 76-77 0.7070284967708282 1103 78-79 0.7352838052607247 1103 80-81 0.5955715839506652 1103 82-83 0.6245688806497682 1103 84-85 0.6721590589563213 1103 86-87 0.609526536029005 1103 88-89 0.6379441506337784 1103 90-91 0.5935189132003131 1103 92-93 0.5219363637341381 1103 94-95 0.6119848598992732 1103 96-97 0.6851889500702342 1103 98-99 0.6547516970915659 1103 100-101 1.0188358166647227 1104 1 0.30513225213101336 1104 2 0.38582186713655275 1104 3 0.3830841693719975 1104 4 0.23133747665606563 1104 5 0.3039303247859806 1104 6 0.2812097731493992 1104 7 0.2723683886203858 1104 8 0.2990588400362242 1104 9 0.41744118638573013 1104 10-11 0.39786113888505525 1104 12-13 0.44798298845082485 1104 14-15 0.5875532797506295 1104 16-17 0.5531686757690863 1104 18-19 0.5095549274752145 1104 20-21 0.5000941718799865 1104 22-23 0.4820838461724364 1104 24-25 0.4436602554747182 1104 26-27 0.5394807478866994 1104 28-29 0.5537188201729819 1104 30-31 0.5812654510892585 1104 32-33 0.5380075441489538 1104 34-35 0.56867732385723 1104 36-37 0.6485897723877585 1104 38-39 0.66769380965831 1104 40-41 0.6051473334611188 1104 42-43 0.671061747373912 1104 44-45 0.7464908185702583 1104 46-47 0.5078763556349202 1104 48-49 0.4762999978600533 1104 50-51 0.5064579252487533 1104 52-53 0.3825117489776346 1104 54-55 0.5584872585928267 1104 56-57 0.8064737605433479 1104 58-59 0.6716831379795423 1104 60-61 0.605825695672614 1104 62-63 0.4818384896504213 1104 64-65 0.5077126650596995 1104 66-67 0.4807706537967462 1104 68-69 0.44735034879698077 1104 70-71 0.5466335798339443 1104 72-73 0.4916183260285436 1104 74-75 0.5714555408034947 1104 76-77 0.5818066764803795 1104 78-79 0.7219015829551978 1104 80-81 0.6604121220903316 1104 82-83 0.7325576159160292 1104 84-85 0.757967905574116 1104 86-87 0.8065250520978289 1104 88-89 0.7617780683532303 1104 90-91 0.7862405697793449 1104 92-93 0.7441884936601433 1104 94-95 0.8303375921675915 1104 96-97 0.8670658534323685 1104 98-99 0.998887727741117 1104 100-101 1.1215500362333124 1105 1 0.29930266517894566 1105 2 0.33452657418940035 1105 3 0.34965951343646395 1105 4 0.19955613380309956 1105 5 0.2522763847060858 1105 6 0.2795340292090813 1105 7 0.3033460297322108 1105 8 0.34294126931170155 1105 9 0.3600641537620888 1105 10-11 0.44365822316017756 1105 12-13 0.4802231325560484 1105 14-15 0.471445815334242 1105 16-17 0.43169710259418537 1105 18-19 0.47646681182475703 1105 20-21 0.5085452157608401 1105 22-23 0.5435801588175764 1105 24-25 0.6217256953347743 1105 26-27 0.46763461513743465 1105 28-29 0.5221478290253714 1105 30-31 0.4635154030040596 1105 32-33 0.6346875220555717 1105 34-35 0.6529732589129225 1105 36-37 0.5740261806377234 1105 38-39 0.6048379258815331 1105 40-41 0.4550633835487261 1105 42-43 0.5129591837202199 1105 44-45 0.637368613038511 1105 46-47 0.6155710423382459 1105 48-49 0.5817332213773412 1105 50-51 0.49677058116093775 1105 52-53 0.5336821887333301 1105 54-55 0.590972934589935 1105 56-57 0.6127640227799418 1105 58-59 0.6299405281191639 1105 60-61 0.6427310579410062 1105 62-63 0.5234756150599935 1105 64-65 0.5112420737879333 1105 66-67 0.47989984599009716 1105 68-69 0.359325313970281 1105 70-71 0.4464996594684223 1105 72-73 0.5192386439497056 1105 74-75 0.611477295288207 1105 76-77 0.5893094824789884 1105 78-79 0.6435039680708492 1105 80-81 0.575863697657713 1105 82-83 0.5804992551847938 1105 84-85 0.6339747490461853 1105 86-87 0.5697304148375331 1105 88-89 0.606698026976396 1105 90-91 0.6167874303488787 1105 92-93 0.590343069227746 1105 94-95 0.7517304672091392 1105 96-97 0.7605248748553635 1105 98-99 0.831037671419157 1105 100-101 0.8896443227241484 1106 1 0.29281044081318086 1106 2 0.3293054677319809 1106 3 0.3232579390306505 1106 4 0.17460811584907532 1106 5 0.25593550669795206 1106 6 0.22217716259753928 1106 7 0.305777508532465 1106 8 0.3098476938656063 1106 9 0.41281295917779204 1106 10-11 0.37702972625968556 1106 12-13 0.40251686378655904 1106 14-15 0.44220208132187366 1106 16-17 0.3568965234488459 1106 18-19 0.4680905536781026 1106 20-21 0.3839953278764483 1106 22-23 0.4180777088361438 1106 24-25 0.4751350317889731 1106 26-27 0.4508458699135076 1106 28-29 0.543318851574 1106 30-31 0.5636737751864516 1106 32-33 0.5085222120329718 1106 34-35 0.5548380137271707 1106 36-37 0.48742040445229406 1106 38-39 0.6029764057932354 1106 40-41 0.5786380662544559 1106 42-43 0.5465563413018373 1106 44-45 0.5262489191212225 1106 46-47 0.5807102714124852 1106 48-49 0.5572570852445438 1106 50-51 0.48839729896215545 1106 52-53 0.4295839203468219 1106 54-55 0.5089837592768376 1106 56-57 0.5888723369313524 1106 58-59 0.5966277676428575 1106 60-61 0.6141851190100454 1106 62-63 0.4114371295845558 1106 64-65 0.3926816044680166 1106 66-67 0.4062302564250544 1106 68-69 0.4821059361504041 1106 70-71 0.43153102984066294 1106 72-73 0.5189797364848658 1106 74-75 0.7242209510202109 1106 76-77 0.6791798734797219 1106 78-79 0.6415706370665184 1106 80-81 0.510229296735428 1106 82-83 0.6379248273754676 1106 84-85 0.7130084199555213 1106 86-87 0.7063014500662881 1106 88-89 0.8031191261880224 1106 90-91 0.6352300262552646 1106 92-93 0.638931111351642 1106 94-95 0.7800261677407967 1106 96-97 0.6652307541638542 1106 98-99 0.8084119279173976 1106 100-101 1.0334615322425194 1107 1 0.18754079142721736 1107 2 0.20772198684851517 1107 3 0.14993077200777805 1107 4 0.15899255899704912 1107 5 0.14904907398735645 1107 6 0.16783973423270027 1107 7 0.15399781385576006 1107 8 0.20766084811162955 1107 9 0.13800304060801238 1107 10-11 0.14255775326827091 1107 12-13 0.20715147170086823 1107 14-15 0.2112977541868588 1107 16-17 0.26711991095745447 1107 18-19 0.30527611549130285 1107 20-21 0.27143100353481486 1107 22-23 0.28421058834066315 1107 24-25 0.23690645905099927 1107 26-27 0.24654048550964092 1107 28-29 0.2945104795527058 1107 30-31 0.4117202211074513 1107 32-33 0.3716169348545293 1107 34-35 0.3059553149927794 1107 36-37 0.27137487292991125 1107 38-39 0.35848478450702714 1107 40-41 0.2979941463141671 1107 42-43 0.3126203317461176 1107 44-45 0.3051886168240969 1107 46-47 0.3112415100916195 1107 48-49 0.3099729837048315 1107 50-51 0.2715330278695163 1107 52-53 0.38991958902172286 1107 54-55 0.40016874204366815 1107 56-57 0.4392354957602791 1107 58-59 0.42137428936364785 1107 60-61 0.4165956723485138 1107 62-63 0.1442900277989736 1107 64-65 0.27674247999195956 1107 66-67 0.2411629386464469 1107 68-69 0.31376507860890257 1107 70-71 0.3406593759067107 1107 72-73 0.26620193243823564 1107 74-75 0.4347582675039696 1107 76-77 0.4303416034607963 1107 78-79 0.4977067827999804 1107 80-81 0.3589353899618928 1107 82-83 0.41023871779603027 1107 84-85 0.4261290580122541 1107 86-87 0.3698674300731817 1107 88-89 0.4442330011867526 1107 90-91 0.4445238661038431 1107 92-93 0.3366061055684497 1107 94-95 0.5714470064269186 1107 96-97 0.6922192619846221 1107 98-99 0.7359502388187451 1107 100-101 0.9716379653459306 1108 1 0.06087055790585438 1108 2 0.07258181868361291 1108 3 0.10219910710147673 1108 4 0.02392244032225932 1108 5 0.10115107757573583 1108 6 0.13975075350755617 1108 7 0.06528950339180994 1108 8 0.08712758411672183 1108 9 0.17275104693609933 1108 10-11 0.0811181526483793 1108 12-13 0.1553219116231972 1108 14-15 0.13707239200504517 1108 16-17 0.13401780820436215 1108 18-19 0.12060605723780071 1108 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-1.1573887013289443 2316 94-95 -1.7090019171268978 2316 96-97 -1.2452937336753855 2316 98-99 -1.3464424299331235 2316 100-101 -1.7037735823210411 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 17.0 4 45.0 5 94.0 6 231.0 7 677.0 8 1724.0 9 3229.0 10 5990.0 11 9188.0 12 13489.0 13 17199.0 14 20443.0 15 25596.0 16 30459.0 17 35057.0 18 30595.0 19 24262.0 20 23823.0 21 25390.0 22 28084.0 23 32805.0 24 40554.0 25 51506.0 26 66688.0 27 85706.0 28 108878.0 29 136381.0 30 167377.0 31 202609.0 32 244648.0 33 295481.0 34 364252.0 35 457920.0 36 601203.0 37 837807.0 38 828287.0 39 161328.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.730254870202906 21.908762830277205 14.913309263508346 23.447673036011544 2 14.606110795655972 22.148967892603224 40.88025441194754 22.36466689979326 3 17.770609386664717 26.940180443414153 31.525016183108228 23.764193986812902 4 11.968563247713302 18.575600625129766 40.237719113294304 29.21811701386262 5 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30.615927693488647 20.58645606590526 26-27 20.61114398868748 28.44552174437429 30.359716391654917 20.58361787528331 28-29 20.574394079359028 28.5322487367333 30.368601032866575 20.524756151041096 30-31 20.865500541050817 28.281838518299068 30.300271906281463 20.552389034368655 32-33 20.69993247280607 28.47464978115183 30.126670489491303 20.698747256550806 34-35 20.849892424909854 28.421326764132047 30.307390974137586 20.42138983682051 36-37 20.90383639557225 28.31969892806993 30.27687535040855 20.49958932594927 38-39 20.691641771530183 28.478044462886153 30.3255650197633 20.504748745820365 40-41 20.90869825748044 28.43837031706467 30.157029513241547 20.495901912213345 42-43 20.8250392991593 28.438108996363948 29.933515825519365 20.803335878957384 44-45 20.7673139032147 28.651573173167705 29.896180302427585 20.684932621190015 46-47 20.893791370209875 28.601279656894153 29.633101952748227 20.871827020147744 48-49 20.95207219736711 28.692274882121083 29.516877457244068 20.838775463267744 50-51 20.980452563933326 28.825063104279824 29.290175452435147 20.9043088793517 52-53 21.00430458476079 28.874065962613976 29.181346718940453 20.940282733684786 54-55 20.96480022128611 28.834759677275013 29.313489764964864 20.886950336474012 56-57 20.942211202889418 28.86379834835207 29.230508737729917 20.9634817110286 58-59 20.84105080327475 28.827033959479152 29.434357538392497 20.8975576988536 60-61 20.927648761716615 28.909034493686924 29.35355807894544 20.809758665651017 62-63 20.900684773616817 28.831419417951487 29.323190880368866 20.944704928062833 64-65 20.709369377295957 28.749044017621582 29.366822305010547 21.174764300071907 66-67 20.720204895474733 28.84059457865453 29.260731384380474 21.178469141490254 68-69 20.805234939220483 28.838758105344713 29.234078650536116 21.121928304898685 70-71 20.830493846273708 28.61232581319722 29.24213327030595 21.315047070223123 72-73 20.86260135280235 28.699418148336314 29.196658056961255 21.241322441900078 74-75 20.71348963814988 28.937043870958906 29.037991906434613 21.3114745844566 76-77 20.654922409104106 28.797093303800775 29.12024739724194 21.42773688985318 78-79 20.592636333873628 28.838257240242065 29.147158836419436 21.421947589464867 80-81 20.645647779204975 28.83045328923935 29.02248747078139 21.501411460774282 82-83 20.705357472810153 28.737690457483577 29.201482447303352 21.355469622402925 84-85 20.74646961758274 28.702191817578566 28.999617197127815 21.55172136771088 86-87 20.53317651671077 28.790200326808414 29.076368570478305 21.60025458600251 88-89 20.620464692270637 28.62330297987059 28.999267632625724 21.756964695233044 90-91 20.50333738522215 28.6310433868113 29.152851279076586 21.71276794888996 92-93 20.552212062288074 28.591638275989524 29.05582584730101 21.800323814421393 94-95 20.27004852457963 28.55578117342717 29.140677094630963 22.03349320736224 96-97 20.327009351660077 28.419908366283813 29.156211636226697 22.096870645829412 98-99 20.21163013771263 28.475773759887403 29.071799438609347 22.240796663790626 100-101 20.169453535845292 28.41896589399132 29.00955882471083 22.402021745452558 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 149.5 2 173.5 3 245.0 4 222.0 5 239.0 6 401.0 7 871.0 8 1570.5 9 2031.0 10 2033.0 11 2042.5 12 2319.0 13 2680.0 14 3123.0 15 3658.5 16 4276.5 17 4931.0 18 5568.5 19 6401.0 20 7502.0 21 9121.0 22 11647.5 23 15127.5 24 19707.0 25 25810.0 26 34640.0 27 45245.5 28 56288.5 29 69836.0 30 83959.5 31 98055.5 32 115126.0 33 130578.5 34 144443.0 35 157373.5 36 171453.0 37 184652.5 38 190978.5 39 194668.0 40 197709.5 41 199673.5 42 200939.5 43 203935.5 44 207350.5 45 210963.0 46 209818.5 47 203474.5 48 197698.5 49 188982.0 50 176674.0 51 162482.5 52 147589.5 53 133348.0 54 119035.5 55 101397.0 56 82043.0 57 64244.0 58 49634.5 59 37504.5 60 26405.0 61 18079.0 62 12466.5 63 7926.0 64 4447.0 65 2526.0 66 1473.0 67 842.5 68 454.0 69 271.5 70 152.5 71 84.5 72 56.0 73 40.5 74 32.5 75 22.5 76 16.5 77 11.5 78 6.0 79 3.0 80 4.0 81 2.5 82 0.0 83 0.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0043381647634977176 2 0.0073708632787206595 3 0.0021489982856215547 4 0.17224120838776122 5 0.001867820939839295 6 6.226069799464317E-4 7 0.0 8 7.23027460582953E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.117239706097371E-4 24-25 7.631956528375614E-4 26-27 0.003123076947795811 28-29 1.707148170820861E-4 30-31 0.006557457385564838 32-33 0.021479940808151892 34-35 0.013677269462694194 36-37 0.0010945832389380817 38-39 2.5105120159130313E-4 40-41 0.001918031180157556 42-43 0.011769280330600288 44-45 0.007722334960948484 46-47 0.010885580100998903 48-49 0.010935790341317163 50-51 0.19103992236291803 52-53 0.11572456188552707 54-55 0.01794513988974635 56-57 0.006818550635219792 58-59 0.018286569523910518 60-61 0.12476240514281399 62-63 0.24143091954632437 64-65 0.24732560175968815 66-67 0.2575684907846133 68-69 0.2528186020505059 70-71 0.23922166897232092 72-73 0.16315315489015605 74-75 0.27390690298417536 76-77 0.2116160788453412 78-79 0.005904724261427449 80-81 5.021024031826062E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.1046252870017336E-4 90-91 0.006346574376228142 92-93 0.01815602289908304 94-95 0.002892109842331812 96-97 3.0126144190956375E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4979064.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.56257825930949 #Duplication Level Percentage of deduplicated Percentage of total 1 71.1355934291768 38.10205790072704 2 16.144968450065463 17.295322722014276 3 5.535848880609868 8.895430166981306 4 2.543123293728391 5.448649617735995 5 1.3785112130454973 3.6918307365042557 6 0.8097631931283196 2.602380264206638 7 0.5157569252517911 1.9337689470105928 8 0.36721339815734777 1.5735117101335931 9 0.2539079162897805 1.2239966373236615 >10 1.1992942528664716 11.286637124405017 >50 0.0755356236846437 2.7696311913247595 >100 0.037934293510840274 3.6517755808569063 >500 0.002099284846633081 0.7463006430011506 >1k 3.373842286558208E-4 0.26711195912454017 >5k 7.497427303318821E-5 0.2920501725487105 >10k+ 3.7487136516594106E-5 0.21954462610158823 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10934 0.21959950705594464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8738 0.1754948319603845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5807 0.11662834621125578 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.0336384509217E-5 0.0 3 0.0 0.0 0.0 8.0336384509217E-5 0.0 4 0.0 0.0 0.0 1.0042048063652124E-4 0.0 5 0.0 0.0 0.0 1.0042048063652124E-4 2.008409612730425E-5 6 0.0 0.0 0.0 1.0042048063652124E-4 2.008409612730425E-5 7 0.0 0.0 0.0 1.4058867289112973E-4 2.008409612730425E-5 8 0.0 0.0 0.0 1.4058867289112973E-4 2.008409612730425E-5 9 0.0 0.0 0.0 1.60672769018434E-4 2.008409612730425E-5 10-11 0.0 0.0 0.0 2.2092505740034673E-4 2.008409612730425E-5 12-13 0.0 2.008409612730425E-5 0.0 2.2092505740034673E-4 2.008409612730425E-5 14-15 0.0 2.008409612730425E-5 0.0 2.3096710546399886E-4 2.008409612730425E-5 16-17 0.0 2.008409612730425E-5 0.0 2.4100915352765097E-4 2.008409612730425E-5 18-19 0.0 2.008409612730425E-5 0.0 2.4100915352765097E-4 2.008409612730425E-5 20-21 0.0 2.008409612730425E-5 0.0 2.6109324965495524E-4 2.008409612730425E-5 22-23 0.0 2.008409612730425E-5 0.0 3.012614419095637E-4 2.008409612730425E-5 24-25 0.0 2.008409612730425E-5 0.0 3.615137302914765E-4 2.008409612730425E-5 26-27 0.0 2.008409612730425E-5 0.0 4.920603551189541E-4 2.008409612730425E-5 28-29 0.0 2.008409612730425E-5 0.0 0.0011146673350653858 2.008409612730425E-5 30-31 0.0 2.008409612730425E-5 0.0 0.004287954523179457 2.008409612730425E-5 32-33 0.0 2.008409612730425E-5 0.0 0.012974326098238544 2.008409612730425E-5 34-35 0.0 2.008409612730425E-5 0.0 0.025446549793294485 2.008409612730425E-5 36-37 0.0 2.008409612730425E-5 0.0 0.0456210243531716 2.008409612730425E-5 38-39 0.0 2.008409612730425E-5 0.0 0.07795641911813143 2.008409612730425E-5 40-41 0.0 2.008409612730425E-5 0.0 0.11623670633677334 2.008409612730425E-5 42-43 0.0 2.008409612730425E-5 0.0 0.15839322410798495 2.008409612730425E-5 44-45 0.0 2.008409612730425E-5 0.0 0.21146544812438645 2.008409612730425E-5 46-47 0.0 2.008409612730425E-5 0.0 0.27463997249282196 2.008409612730425E-5 48-49 0.0 2.008409612730425E-5 0.0 0.34358867449785746 2.008409612730425E-5 50-51 0.0 2.008409612730425E-5 0.0 0.4175182323424643 2.008409612730425E-5 52-53 0.0 2.008409612730425E-5 0.0 0.49538427302802296 2.008409612730425E-5 54-55 0.0 2.008409612730425E-5 0.0 0.5813241203567578 2.008409612730425E-5 56-57 0.0 2.008409612730425E-5 0.0 0.6845563744511016 2.008409612730425E-5 58-59 0.0 2.008409612730425E-5 0.0 0.8018173696903675 2.008409612730425E-5 60-61 0.0 2.008409612730425E-5 0.0 0.9277948626488834 2.008409612730425E-5 62-63 0.0 2.008409612730425E-5 0.0 1.060299686848773 2.008409612730425E-5 64-65 0.0 2.008409612730425E-5 0.0 1.2036097547651528 2.008409612730425E-5 66-67 0.0 2.008409612730425E-5 0.0 1.366039882194726 2.008409612730425E-5 68-69 0.0 2.008409612730425E-5 0.0 1.5478712464832747 2.008409612730425E-5 70-71 0.0 2.008409612730425E-5 0.0 1.7548579411712724 2.008409612730425E-5 72-73 0.0 2.008409612730425E-5 0.0 1.9727503000563962 2.008409612730425E-5 74-75 0.0 2.008409612730425E-5 0.0 2.1948904452724447 2.008409612730425E-5 76-77 0.0 2.008409612730425E-5 0.0 2.4461926980653392 2.008409612730425E-5 78-79 0.0 2.008409612730425E-5 0.0 2.7371811248057867 2.008409612730425E-5 80-81 0.0 2.008409612730425E-5 0.0 3.053154167128601 2.008409612730425E-5 82-83 0.0 2.008409612730425E-5 0.0 3.3862087332076873 2.008409612730425E-5 84-85 0.0 2.008409612730425E-5 0.0 3.7232700764641704 2.008409612730425E-5 86-87 0.0 2.008409612730425E-5 0.0 4.086059146859731 2.008409612730425E-5 88-89 0.0 2.008409612730425E-5 0.0 4.475499812816224 2.008409612730425E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5900 0.0 53.80831 1 GTATCAA 11545 0.0 44.2115 1 ATCAACG 14295 0.0 35.40532 3 CAACGCA 14430 0.0 35.075726 5 TCAACGC 14515 0.0 34.867638 4 TATCAAC 15185 0.0 33.519653 2 AACGCAG 15265 0.0 33.34352 6 ACGCAGA 17200 0.0 29.17869 7 CGCAGAG 17230 0.0 29.100357 8 GCAGAGT 20435 0.0 24.071789 9 GTACATG 11660 0.0 23.518097 1 ACATGGG 11125 0.0 22.17024 3 AGAGTAC 18740 0.0 21.4017 10-11 TACATGG 12195 0.0 21.395157 2 CAGAGTA 19790 0.0 19.858704 10-11 GAGTACT 15145 0.0 19.826162 12-13 ACGCGTC 120 0.004802967 19.765024 8 AGTACTT 16180 0.0 19.158932 12-13 GTGGTAT 3095 0.0 18.580166 1 GTACTTT 16390 0.0 18.117556 14-15 >>END_MODULE