FastQCFastQC Report
Sat 18 Jun 2016
SRR1930981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993512
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN878444.40649466870528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.054.8937031
GTATCAA29250.042.1690751
ATCAACG34100.036.0093353
TCAACGC34850.035.3708344
TATCAAC35950.033.594362
CAACGCA36300.033.533345
AACGCAG38750.031.7456286
ACGCAGA44250.027.325697
CGCAGAG45450.026.5611768
GTACATG28150.024.5447311
GTGGTAT7200.024.168481
GCAGAGT52100.023.0973389
TACATGG30050.021.6278062
GTATAGG4300.021.4243161
ACATGGG31000.020.6168213
AGAGTAC44700.020.1847810-11
TGGTATC8750.019.8849322
GAGTACT33950.019.86465312-13
CAGAGTA48450.019.85138710-11
TAACGCA2301.186545E-619.6877754
Produced by FastQC (version 0.11.5)