##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930981_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1993512 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.06776031446011 28.0 2.0 31.0 2.0 33.0 2 19.77170942537592 27.0 2.0 31.0 2.0 33.0 3 20.411842015498276 27.0 2.0 31.0 2.0 33.0 4 21.927146162150013 30.0 2.0 35.0 2.0 37.0 5 21.262278330905456 30.0 2.0 35.0 2.0 37.0 6 22.23644502766976 30.0 2.0 35.0 2.0 37.0 7 22.412949106902794 30.0 2.0 35.0 2.0 37.0 8 22.83654123978185 31.0 2.0 35.0 2.0 37.0 9 24.14621532250621 32.0 2.0 37.0 2.0 39.0 10-11 24.489772823037935 32.0 2.0 37.0 2.0 39.0 12-13 24.38190816007127 32.0 2.0 37.0 2.0 39.0 14-15 24.321821739723664 32.0 2.0 38.0 2.0 40.0 16-17 23.147862415676457 31.0 2.0 38.0 2.0 40.0 18-19 22.098657294262587 28.5 2.0 37.0 2.0 40.0 20-21 21.75080987724177 27.0 2.0 37.0 2.0 40.0 22-23 21.37318937633684 27.0 2.0 37.0 2.0 39.5 24-25 21.188407192933877 27.0 2.0 37.0 2.0 39.0 26-27 20.8974894557946 26.5 2.0 37.0 2.0 39.0 28-29 20.690942918828682 25.5 2.0 36.0 2.0 39.0 30-31 20.665207182098726 25.0 2.0 36.0 2.0 39.0 32-33 20.529903256162992 25.0 2.0 36.0 2.0 39.0 34-35 20.199633862249136 25.0 2.0 36.0 2.0 39.0 36-37 19.723993384539448 24.0 2.0 36.0 2.0 39.0 38-39 19.58737670001485 24.0 2.0 35.0 2.0 39.0 40-41 19.445284001300216 24.0 2.0 35.0 2.0 39.0 42-43 19.287133962574593 23.5 2.0 35.0 2.0 38.0 44-45 18.898576732921597 21.5 2.0 35.0 2.0 38.0 46-47 18.542016551693695 19.0 2.0 35.0 2.0 38.0 48-49 18.280863119961154 17.0 2.0 34.5 2.0 38.0 50-51 16.084913208448206 10.0 2.0 32.5 2.0 37.5 52-53 17.98034674484026 17.5 2.0 33.5 2.0 38.0 54-55 18.717239224042793 21.0 2.0 35.0 2.0 39.0 56-57 18.66834536235548 20.0 2.0 35.0 2.0 39.0 58-59 18.379709276894246 18.5 2.0 35.0 2.0 39.0 60-61 18.077923784757754 16.0 2.0 35.0 2.0 39.0 62-63 17.62698744727897 12.5 2.0 34.0 2.0 38.0 64-65 17.256866023379843 8.5 2.0 34.0 2.0 38.0 66-67 16.8785231791933 8.0 2.0 33.5 2.0 38.0 68-69 16.416545774492455 7.0 2.0 33.0 2.0 37.5 70-71 16.049027043729858 2.0 2.0 32.0 2.0 37.0 72-73 15.638779701351183 2.0 2.0 32.0 2.0 36.0 74-75 15.172492315070087 2.0 2.0 31.0 2.0 36.0 76-77 14.739052737079085 2.0 2.0 31.0 2.0 35.0 78-79 14.339458453222253 2.0 2.0 30.5 2.0 35.0 80-81 13.892161923279117 2.0 2.0 30.0 2.0 34.0 82-83 13.413192396133056 2.0 2.0 29.5 2.0 34.0 84-85 12.998439939162644 2.0 2.0 29.0 2.0 34.0 86-87 12.50117807166448 2.0 2.0 29.0 2.0 34.0 88-89 12.071315346985621 2.0 2.0 28.5 2.0 34.0 90-91 11.572391337498846 2.0 2.0 26.5 2.0 34.0 92-93 11.018479948954408 2.0 2.0 26.0 2.0 33.0 94-95 10.447772072603525 2.0 2.0 25.0 2.0 33.0 96-97 9.80386950266665 2.0 2.0 22.0 2.0 32.0 98-99 9.135628729598817 2.0 2.0 13.0 2.0 32.0 100-101 8.23576130968863 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.720252147221704 1101 2 9.522783384254204 1101 3 8.752079140048018 1101 4 10.827498725445931 1101 5 11.610276145747957 1101 6 10.873116796773793 1101 7 10.453672105155576 1101 8 9.797179117907039 1101 9 9.474707614656204 1101 10-11 9.142752860712374 1101 12-13 9.108150313108897 1101 14-15 9.60369927856527 1101 16-17 10.755724984833794 1101 18-19 11.585753900438835 1101 20-21 11.587781952868372 1101 22-23 11.837688618154836 1101 24-25 11.760014277636852 1101 26-27 11.639863892058258 1101 28-29 11.73816871418698 1101 30-31 11.276193971403984 1101 32-33 11.228355745202276 1101 34-35 11.158258896178932 1101 36-37 11.455763830447413 1101 38-39 11.226835938225666 1101 40-41 10.909879165206075 1101 42-43 10.587788746937235 1101 44-45 10.376294844350088 1101 46-47 10.22957286549028 1101 48-49 10.646759403858542 1101 50-51 12.146215407608997 1101 52-53 11.182834475966263 1101 54-55 10.92333404829241 1101 56-57 10.907512581988883 1101 58-59 10.746806564661714 1101 60-61 10.479145926362637 1101 62-63 10.340967589012074 1101 64-65 10.160609287776229 1101 66-67 9.837944088796416 1101 68-69 9.750443558183733 1101 70-71 9.502751930609472 1101 72-73 9.587731073441098 1101 74-75 9.231453417642983 1101 76-77 9.073730861106881 1101 78-79 8.522699641475592 1101 80-81 8.383624073424292 1101 82-83 8.219696701422256 1101 84-85 7.821632823111058 1101 86-87 7.669491821523261 1101 88-89 7.2404530653015335 1101 90-91 6.915559432664002 1101 92-93 6.767006591133109 1101 94-95 6.4665733411390445 1101 96-97 6.194836488635589 1101 98-99 5.705548948724491 1101 100-101 4.9679083631378 1102 1 7.7085182162982555 1102 2 8.96257165286983 1102 3 8.501948629422543 1102 4 10.177424946268491 1102 5 10.941609023532003 1102 6 10.072645968197552 1102 7 9.664739972095575 1102 8 9.37877195588004 1102 9 9.283451189925813 1102 10-11 8.191280177399765 1102 12-13 8.5179819720864 1102 14-15 8.978596532395347 1102 16-17 9.954442090143491 1102 18-19 10.986397116220648 1102 20-21 10.82965952329106 1102 22-23 10.986847408204618 1102 24-25 11.038664366270897 1102 26-27 11.261848177022724 1102 28-29 11.000948498550887 1102 30-31 11.043741107314705 1102 32-33 10.882036781477861 1102 34-35 10.56441786590188 1102 36-37 10.492785344540437 1102 38-39 10.640306686161193 1102 40-41 10.28883851804314 1102 42-43 10.169050533567571 1102 44-45 10.152050002172452 1102 46-47 10.086104550923597 1102 48-49 10.00944878574041 1102 50-51 11.147114480806712 1102 52-53 10.726162588874576 1102 54-55 10.521488649269436 1102 56-57 10.3467154980702 1102 58-59 10.314867679003168 1102 60-61 10.267435409540564 1102 62-63 10.109192389850737 1102 64-65 9.825777027530261 1102 66-67 9.651562536561745 1102 68-69 9.691911134251267 1102 70-71 9.394977741579156 1102 72-73 9.062212886131118 1102 74-75 8.763389261590477 1102 76-77 8.543301725193327 1102 78-79 8.184960425048656 1102 80-81 7.798219547075293 1102 82-83 7.853940142998061 1102 84-85 7.6051645043001255 1102 86-87 7.188079859251795 1102 88-89 6.753344968549655 1102 90-91 6.725398377600547 1102 92-93 6.551084011875316 1102 94-95 6.3309494579067955 1102 96-97 6.258524966492638 1102 98-99 5.590718343735237 1102 100-101 4.724464665209419 1103 1 7.736958236092743 1103 2 9.045789587672779 1103 3 8.29250549490736 1103 4 10.219246343997671 1103 5 11.022002639948674 1103 6 10.170082526729214 1103 7 9.817154746988734 1103 8 9.187022569606157 1103 9 8.982331720218454 1103 10-11 8.461254519420219 1103 12-13 8.314043312816327 1103 14-15 8.845434252259 1103 16-17 9.723344230803558 1103 18-19 10.438004933102238 1103 20-21 10.97412947323652 1103 22-23 11.074987974010888 1103 24-25 11.05496674784705 1103 26-27 11.102958262369121 1103 28-29 10.648734856711883 1103 30-31 10.433015004513674 1103 32-33 10.78532207020984 1103 34-35 10.67864439079123 1103 36-37 10.76593227979112 1103 38-39 10.26627301889986 1103 40-41 10.06610514617432 1103 42-43 9.753513247713283 1103 44-45 9.854287832652357 1103 46-47 9.64295062938351 1103 48-49 9.929448841187156 1103 50-51 11.474104013848118 1103 52-53 10.724230269939373 1103 54-55 10.21440380327969 1103 56-57 10.05086312224664 1103 58-59 10.053920413012676 1103 60-61 9.734703465425333 1103 62-63 9.855548845861371 1103 64-65 9.533154077846792 1103 66-67 9.13571065975886 1103 68-69 9.240178908743825 1103 70-71 9.062527397601437 1103 72-73 8.841721641864659 1103 74-75 8.723640878902613 1103 76-77 8.341729948693597 1103 78-79 7.8747944729518675 1103 80-81 7.590538579307873 1103 82-83 7.134108368411239 1103 84-85 7.023873690300379 1103 86-87 6.841667679266125 1103 88-89 6.452839571502473 1103 90-91 6.035045902665937 1103 92-93 5.680788494605734 1103 94-95 5.456883908769202 1103 96-97 5.24899367165875 1103 98-99 4.5737201055554415 1103 100-101 3.7773553117600063 1104 1 8.08705494789158 1104 2 9.722802881707349 1104 3 8.888121226693308 1104 4 10.796614759663957 1104 5 11.58769809500583 1104 6 10.819087265095654 1104 7 10.408204044421012 1104 8 9.653531631859902 1104 9 9.55546119122026 1104 10-11 9.061708911363969 1104 12-13 8.933524855702121 1104 14-15 9.585824988394418 1104 16-17 10.561286981512264 1104 18-19 11.408232026733582 1104 20-21 11.61553031038203 1104 22-23 11.902125611786822 1104 24-25 11.522674217682177 1104 26-27 11.729533027241757 1104 28-29 11.666326170047878 1104 30-31 11.358490632116961 1104 32-33 11.196821051921585 1104 34-35 11.125294273611328 1104 36-37 11.334819735912614 1104 38-39 10.974952519068719 1104 40-41 10.497675152212413 1104 42-43 10.121496013568386 1104 44-45 10.33385759307318 1104 46-47 10.28704404581735 1104 48-49 10.034280337508012 1104 50-51 11.743368083112186 1104 52-53 10.836890816642473 1104 54-55 10.623485501723092 1104 56-57 10.460118799587423 1104 58-59 10.524845469044113 1104 60-61 10.052800340969018 1104 62-63 10.022229840201941 1104 64-65 9.86076248205094 1104 66-67 9.617055004932993 1104 68-69 9.352508895541899 1104 70-71 8.875737512939212 1104 72-73 8.89503286964397 1104 74-75 8.689869528109986 1104 76-77 8.314103123194432 1104 78-79 8.016017237578888 1104 80-81 7.771248413209195 1104 82-83 7.729274825279829 1104 84-85 7.109202152224585 1104 86-87 7.006094111777665 1104 88-89 6.737627675439512 1104 90-91 6.357157597118059 1104 92-93 6.015286463146255 1104 94-95 5.568776901513257 1104 96-97 5.3495278892142455 1104 98-99 4.769315369235812 1104 100-101 4.069118946076832 1105 1 7.863385474943222 1105 2 8.99148788244996 1105 3 8.34215169712796 1105 4 10.513413923474651 1105 5 10.415890003132212 1105 6 10.411809693350094 1105 7 9.886837107176525 1105 8 9.626550902852014 1105 9 9.471131588801029 1105 10-11 8.86909737199618 1105 12-13 8.582632663597455 1105 14-15 9.305718767338039 1105 16-17 10.0959687371078 1105 18-19 10.750411791801017 1105 20-21 11.407307782182418 1105 22-23 11.546830360354924 1105 24-25 11.489814925964009 1105 26-27 11.580085405769296 1105 28-29 11.365745483028132 1105 30-31 11.057988449515463 1105 32-33 10.837074722812751 1105 34-35 10.814820466504386 1105 36-37 10.910509050489757 1105 38-39 10.91460361550632 1105 40-41 10.747536959868771 1105 42-43 10.381547873386072 1105 44-45 10.157586809782227 1105 46-47 10.242236671985562 1105 48-49 10.028689214872497 1105 50-51 11.580842496590384 1105 52-53 10.685791810622312 1105 54-55 10.556920678626046 1105 56-57 10.382882735796723 1105 58-59 10.18753777974294 1105 60-61 9.968228971742526 1105 62-63 9.943292921796282 1105 64-65 9.877297879949971 1105 66-67 9.67265556373258 1105 68-69 9.249586364773858 1105 70-71 9.161431729854849 1105 72-73 8.903583597680324 1105 74-75 8.795398561902617 1105 76-77 8.656806734956024 1105 78-79 8.200426572792756 1105 80-81 8.08595464699602 1105 82-83 7.990620835457483 1105 84-85 7.914741906392971 1105 86-87 7.528527391397448 1105 88-89 7.170487972192916 1105 90-91 6.72681513694816 1105 92-93 6.5279464452119615 1105 94-95 6.254564586704957 1105 96-97 5.7019267407939935 1105 98-99 5.306413006174655 1105 100-101 4.881197794440467 1106 1 7.801013070147576 1106 2 8.991114262997453 1106 3 8.506517038918904 1106 4 10.355672510423837 1106 5 11.183118561064596 1106 6 10.335777572424256 1106 7 9.501777161611148 1106 8 9.218239500823088 1106 9 8.87829131617805 1106 10-11 8.510965919131618 1106 12-13 8.338901417674425 1106 14-15 9.04172333354808 1106 16-17 9.813591946051279 1106 18-19 11.091227636324934 1106 20-21 11.14412706823411 1106 22-23 11.304482923505837 1106 24-25 11.113256791137097 1106 26-27 11.23065427006513 1106 28-29 11.316430864407891 1106 30-31 10.959549396048068 1106 32-33 11.030569785457555 1106 34-35 10.766388498535338 1106 36-37 11.116244930103768 1106 38-39 10.721828574455415 1106 40-41 10.521398556467734 1106 42-43 10.129484868684901 1106 44-45 9.665281098645622 1106 46-47 9.597248368681246 1106 48-49 9.900600837339152 1106 50-51 11.615987130731236 1106 52-53 10.915259610968715 1106 54-55 10.086048824924713 1106 56-57 10.224792896176414 1106 58-59 10.173227772320036 1106 60-61 9.932577443299312 1106 62-63 9.72059694590947 1106 64-65 9.718243062935777 1106 66-67 9.639056018104217 1106 68-69 9.182581506146423 1106 70-71 8.817113737187777 1106 72-73 8.775502305645322 1106 74-75 8.434494654756387 1106 76-77 8.525161887125535 1106 78-79 7.923536656694052 1106 80-81 7.707082590851881 1106 82-83 7.716645645948523 1106 84-85 7.486365272791959 1106 86-87 7.310292017890463 1106 88-89 7.053585122248023 1106 90-91 6.78513969775973 1106 92-93 6.428734622551863 1106 94-95 6.354327391212683 1106 96-97 5.8077599054249855 1106 98-99 5.229783116618453 1106 100-101 4.713865653270348 1107 1 7.413382948331407 1107 2 8.445876167759359 1107 3 8.110071856194654 1107 4 9.796901746304236 1107 5 10.910228195616092 1107 6 9.556242688703328 1107 7 9.628277715321005 1107 8 8.228383465385658 1107 9 5.610251449068411 1107 10-11 7.543789839397398 1107 12-13 7.604837187331128 1107 14-15 8.268971395563593 1107 16-17 9.277506674733448 1107 18-19 10.596211025029259 1107 20-21 10.958960955277295 1107 22-23 10.305125226938841 1107 24-25 10.452276724691913 1107 26-27 10.410155931194694 1107 28-29 10.569575936390173 1107 30-31 10.233907092615066 1107 32-33 10.505099132079923 1107 34-35 10.488071776497687 1107 36-37 10.97033130884131 1107 38-39 10.684010191288195 1107 40-41 10.053607858793313 1107 42-43 9.712203584078036 1107 44-45 9.114721851691026 1107 46-47 9.715183030918237 1107 48-49 9.951890981303713 1107 50-51 11.154918470709086 1107 52-53 10.232778990813674 1107 54-55 10.185317927914749 1107 56-57 9.99597783526833 1107 58-59 9.77186564607419 1107 60-61 9.625168805425556 1107 62-63 9.794943568279347 1107 64-65 9.701620693057592 1107 66-67 9.56513021522493 1107 68-69 9.391335658970345 1107 70-71 9.040973094884345 1107 72-73 8.643885472865698 1107 74-75 8.830491332497251 1107 76-77 8.636341289118889 1107 78-79 8.347019491688512 1107 80-81 8.171285248659194 1107 82-83 7.891933575073656 1107 84-85 7.60791566038886 1107 86-87 7.080790356760895 1107 88-89 7.0962983005426 1107 90-91 6.7322216468184255 1107 92-93 6.603319340159834 1107 94-95 6.2755067932060395 1107 96-97 5.8839448445169005 1107 98-99 5.5180943015575465 1107 100-101 4.836905520379984 1108 1 6.495118114139785 1108 2 7.801710676457056 1108 3 7.816703753943422 1108 4 9.056973470864431 1108 5 10.843313335730457 1108 6 9.351179547036388 1108 7 8.694803556936556 1108 8 5.947509431644502 1108 9 6.989803170567193 1108 10-11 5.949084664514114 1108 12-13 4.973471905954369 1108 14-15 7.178016234347332 1108 16-17 8.287205016102991 1108 18-19 8.021004652914367 1108 20-21 8.236901935585845 1108 22-23 9.215026277659913 1108 24-25 9.020850652376936 1108 26-27 7.5975237710390005 1108 28-29 8.974518042861785 1108 30-31 9.397522732067948 1108 32-33 9.932365070567368 1108 34-35 9.546949986882442 1108 36-37 9.193026446659612 1108 38-39 8.9307344781427 1108 40-41 9.351574861122174 1108 42-43 8.607005521515852 1108 44-45 7.798130517679805 1108 46-47 8.729618910692128 1108 48-49 9.542499218694513 1108 50-51 11.086856564541433 1108 52-53 10.276119977682395 1108 54-55 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-14.529753505705305 2313 68-69 -14.090989922425006 2313 70-71 -13.716933881859843 2313 72-73 -13.304021503878488 2313 74-75 -12.85764820238647 2313 76-77 -12.434123827160178 2313 78-79 -12.036701438544045 2313 80-81 -11.612203123433831 2313 82-83 -11.135973401670528 2313 84-85 -10.726968060541372 2313 86-87 -10.248279410680963 2313 88-89 -9.82974821108531 2313 90-91 -9.360336492716458 2313 92-93 -8.83412252030528 2313 94-95 -8.274958323073031 2313 96-97 -7.636287713622634 2313 98-99 -6.962835931000594 2313 100-101 -6.095420061015366 2314 1 -18.66708216794766 2314 2 -17.37412383224064 2314 3 -18.02062581822395 2314 4 -19.47147034671902 2314 5 -18.771167153221118 2314 6 -19.78422242757574 2314 7 -19.95869902886504 2314 8 -20.405570022986435 2314 9 -21.70742296953624 2314 10-11 -22.06760550943981 2314 12-13 -21.985860487087475 2314 14-15 -21.90660999978525 2314 16-17 -20.728074720615393 2314 18-19 -19.644486649389346 2314 20-21 -19.288015788908748 2314 22-23 -18.927898028875113 2314 24-25 -18.75888606600576 2314 26-27 -18.460774301363443 2314 28-29 -18.248331040354014 2314 30-31 -18.22306965157098 2314 32-33 -18.09466830978054 2314 34-35 -17.76123054908371 2314 36-37 -17.298040784181946 2314 38-39 -17.15436190173506 2314 40-41 -17.023601443532268 2314 42-43 -16.863610369410335 2314 44-45 -16.498766520401997 2314 46-47 -16.141561155128276 2314 48-49 -15.88392297218466 2314 50-51 -13.789965250221146 2314 52-53 -15.601407055697951 2314 54-55 -16.335379746503857 2314 56-57 -16.294254722871205 2314 58-59 -16.015343656251392 2314 60-61 -15.715993985272117 2314 62-63 -15.282498292185767 2314 64-65 -14.886518841826128 2314 66-67 -14.529753505705305 2314 68-69 -14.090989922425006 2314 70-71 -13.716933881859843 2314 72-73 -13.304021503878488 2314 74-75 -12.85764820238647 2314 76-77 -12.434123827160178 2314 78-79 -12.036701438544045 2314 80-81 -11.612203123433831 2314 82-83 -11.135973401670528 2314 84-85 -10.726968060541372 2314 86-87 -10.248279410680963 2314 88-89 -9.82974821108531 2314 90-91 -9.360336492716458 2314 92-93 -8.83412252030528 2314 94-95 -8.274958323073031 2314 96-97 -7.636287713622634 2314 98-99 -6.962835931000594 2314 100-101 -6.095420061015366 2315 1 -18.66708216794766 2315 2 -17.37412383224064 2315 3 -18.02062581822395 2315 4 -19.47147034671902 2315 5 -18.771167153221118 2315 6 -19.78422242757574 2315 7 -19.95869902886504 2315 8 -20.405570022986435 2315 9 -21.70742296953624 2315 10-11 -22.06760550943981 2315 12-13 -21.985860487087475 2315 14-15 -21.90660999978525 2315 16-17 -20.728074720615393 2315 18-19 -19.644486649389346 2315 20-21 -19.288015788908748 2315 22-23 -18.927898028875113 2315 24-25 -18.75888606600576 2315 26-27 -18.460774301363443 2315 28-29 -18.248331040354014 2315 30-31 -18.22306965157098 2315 32-33 -18.09466830978054 2315 34-35 -17.76123054908371 2315 36-37 -17.298040784181946 2315 38-39 -17.15436190173506 2315 40-41 -17.023601443532268 2315 42-43 -16.863610369410335 2315 44-45 -16.498766520401997 2315 46-47 -16.141561155128276 2315 48-49 -15.88392297218466 2315 50-51 -13.789965250221146 2315 52-53 -15.601407055697951 2315 54-55 -16.335379746503857 2315 56-57 -16.294254722871205 2315 58-59 -16.015343656251392 2315 60-61 -15.715993985272117 2315 62-63 -15.282498292185767 2315 64-65 -14.886518841826128 2315 66-67 -14.529753505705305 2315 68-69 -14.090989922425006 2315 70-71 -13.716933881859843 2315 72-73 -13.304021503878488 2315 74-75 -12.85764820238647 2315 76-77 -12.434123827160178 2315 78-79 -12.036701438544045 2315 80-81 -11.612203123433831 2315 82-83 -11.135973401670528 2315 84-85 -10.726968060541372 2315 86-87 -10.248279410680963 2315 88-89 -9.82974821108531 2315 90-91 -9.360336492716458 2315 92-93 -8.83412252030528 2315 94-95 -8.274958323073031 2315 96-97 -7.636287713622634 2315 98-99 -6.962835931000594 2315 100-101 -6.095420061015366 2316 1 -18.66708216794766 2316 2 -17.37412383224064 2316 3 -18.02062581822395 2316 4 -19.47147034671902 2316 5 -18.771167153221118 2316 6 -19.78422242757574 2316 7 -19.95869902886504 2316 8 -20.405570022986435 2316 9 -21.70742296953624 2316 10-11 -22.06760550943981 2316 12-13 -21.985860487087475 2316 14-15 -21.90660999978525 2316 16-17 -20.728074720615393 2316 18-19 -19.644486649389346 2316 20-21 -19.288015788908748 2316 22-23 -18.927898028875113 2316 24-25 -18.75888606600576 2316 26-27 -18.460774301363443 2316 28-29 -18.248331040354014 2316 30-31 -18.22306965157098 2316 32-33 -18.09466830978054 2316 34-35 -17.76123054908371 2316 36-37 -17.298040784181946 2316 38-39 -17.15436190173506 2316 40-41 -17.023601443532268 2316 42-43 -16.863610369410335 2316 44-45 -16.498766520401997 2316 46-47 -16.141561155128276 2316 48-49 -15.88392297218466 2316 50-51 -13.789965250221146 2316 52-53 -15.601407055697951 2316 54-55 -16.335379746503857 2316 56-57 -16.294254722871205 2316 58-59 -16.015343656251392 2316 60-61 -15.715993985272117 2316 62-63 -15.282498292185767 2316 64-65 -14.886518841826128 2316 66-67 -14.529753505705305 2316 68-69 -14.090989922425006 2316 70-71 -13.716933881859843 2316 72-73 -13.304021503878488 2316 74-75 -12.85764820238647 2316 76-77 -12.434123827160178 2316 78-79 -12.036701438544045 2316 80-81 -11.612203123433831 2316 82-83 -11.135973401670528 2316 84-85 -10.726968060541372 2316 86-87 -10.248279410680963 2316 88-89 -9.82974821108531 2316 90-91 -9.360336492716458 2316 92-93 -8.83412252030528 2316 94-95 -8.274958323073031 2316 96-97 -7.636287713622634 2316 98-99 -6.962835931000594 2316 100-101 -6.095420061015366 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 509982.0 3 22118.0 4 77424.0 5 42523.0 6 24839.0 7 24526.0 8 24292.0 9 23895.0 10 25391.0 11 26706.0 12 27640.0 13 27888.0 14 28512.0 15 29721.0 16 30978.0 17 30961.0 18 30593.0 19 31764.0 20 33282.0 21 35329.0 22 37332.0 23 39724.0 24 42420.0 25 44897.0 26 47997.0 27 51370.0 28 54876.0 29 59355.0 30 63636.0 31 69299.0 32 74194.0 33 78740.0 34 78980.0 35 71985.0 36 49914.0 37 18584.0 38 1843.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.78173713647827 20.669215643153578 14.367186364647678 23.181860855720473 2 14.787846244608282 20.930925944897606 41.809992475439195 22.471235335054914 3 18.39589935804003 26.36877055470826 30.56253212561595 24.67279796163576 4 12.284736286255733 17.588650274851396 39.88227829208027 30.244335146812595 5 12.595012175168574 37.97054881458535 36.049224369273006 13.385214640973068 6 29.35169482758736 38.83190409736557 18.39673294610816 13.419668128938907 7 25.814043019456484 33.144526815592826 23.422340240006054 17.61908992494464 8 24.062576420825366 37.009288394291744 21.105813642950647 17.822321541932244 9 25.14647783010616 17.190329169023716 21.631237093025632 36.031955907844484 10-11 23.712093662900266 27.260047452142157 29.535582591720868 19.492276293236706 12-13 24.37972588916455 24.800322057850057 29.687899737557238 21.132052315428155 14-15 21.668803457425092 26.592355794671136 27.184434855721435 24.554405892182338 16-17 21.192475355225575 29.628248186305107 27.86785377894122 21.311422679528096 18-19 21.054285871785925 28.582659021286837 29.908160257149913 20.454894849777325 20-21 21.662879571064313 27.714565588844348 30.075585755260516 20.546969084830824 22-23 21.331083426719406 27.685131244357862 29.79478651751519 21.188998811407544 24-25 21.275509030728056 27.845068068132324 29.91970359701661 20.959719304123013 26-27 21.167593133362182 27.99382495591755 29.72680086778347 21.111781042936794 28-29 21.25012599028363 28.076850713272993 29.810510613421492 20.862512683021887 30-31 21.419253425658187 27.933275814067592 29.741618129043463 20.905852631230758 32-33 21.210656403363583 28.0413346781143 29.619956603958947 21.12805231456317 34-35 21.36750862729744 28.079940216754018 29.694001734403123 20.858549421545423 36-37 21.448925124002944 27.98485909570904 29.500879531725175 21.065336248562836 38-39 21.413096280869468 28.028424751298964 29.55783298214407 21.0006459856875 40-41 21.54391020865185 28.037428769882293 29.497888857288213 20.92077216417764 42-43 21.429463300938547 28.03107858728568 29.251706616954532 21.28775149482124 44-45 21.430348841584035 28.174645868137077 29.307716018621257 21.087289271657635 46-47 21.529107408660177 28.24082550381158 28.896770992479592 21.333296095048652 48-49 21.52887122295882 28.297927060364223 28.90261688480797 21.270584831868984 50-51 21.763939238338555 28.491088205706518 28.525297071469247 21.219675484485684 52-53 21.280777015121394 28.695889679361226 28.690914903081726 21.332418402435653 54-55 21.19837778169366 28.63946227456478 28.75981599398329 21.40234394975827 56-57 21.225714519125045 28.655394266169004 28.856548424984364 21.262342789721586 58-59 21.26689495574426 28.592659844079467 28.83654473306459 21.303900467111685 60-61 21.223883519532908 28.72556552949278 28.871169409801578 21.179381541172738 62-63 21.294922574532066 28.584588840721224 28.810338436927424 21.310150147819286 64-65 21.073360666189135 28.469759275680396 28.90015329942389 21.556726758706578 66-67 21.06761637506379 28.61666773318296 28.662013623906507 21.653702267846743 68-69 21.15750368537433 28.58438849110245 28.695147151732126 21.562960671791092 70-71 21.228598849483443 28.47515572247418 28.64690062095365 21.64934480708872 72-73 21.13731082048677 28.594237624317277 28.597909527525907 21.670542027670045 74-75 21.119230216985795 28.818991419550617 28.345601277739142 21.716177085724446 76-77 21.20684580854192 28.677175375405955 28.398108855157943 21.71786996089418 78-79 21.026426353188597 28.70053669869333 28.430334482534725 21.842702465583343 80-81 21.136580057135085 28.80069583653103 28.174288955680527 21.888435150653358 82-83 21.328602481621076 28.614159532598077 28.2507169751095 21.80652101067134 84-85 21.363582875198478 28.63727549363231 28.17444302007241 21.824698611096803 86-87 21.269808131008634 28.66759130071696 28.23027843408832 21.832322134186086 88-89 21.31545316006517 28.63115602102256 28.103804048793315 21.94958677011896 90-91 21.297035515582913 28.619676361605688 28.200515161302125 21.88277296150927 92-93 21.27755955357563 28.558740483706984 28.130912889733793 22.032787072983588 94-95 21.168424835567635 28.545817039969034 28.14540666256457 22.14035146189876 96-97 21.17454640857558 28.476727951825314 28.115752723285198 22.23297291631391 98-99 21.09404935465196 28.453174450022267 27.838711156702146 22.614065038623625 100-101 21.40643121379653 28.515150848453803 27.79502588263396 22.28339205511571 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 118743.0 1 107129.0 2 70860.5 3 36295.5 4 22546.5 5 17647.5 6 16434.0 7 16537.5 8 17082.0 9 16917.0 10 15644.0 11 13575.0 12 10835.0 13 8284.5 14 6298.0 15 4925.0 16 4473.0 17 4740.0 18 5534.0 19 6467.5 20 7447.0 21 8623.5 22 9869.5 23 11258.5 24 12936.5 25 14952.5 26 17021.0 27 19354.0 28 21857.5 29 24766.0 30 28238.0 31 31877.5 32 35666.5 33 38922.0 34 42067.5 35 45107.0 36 48531.0 37 52058.0 38 54544.5 39 56517.5 40 57468.5 41 57933.0 42 58817.5 43 59856.5 44 60184.5 45 59995.5 46 59790.5 47 58831.5 48 57350.0 49 55072.5 50 51703.0 51 47928.5 52 43990.0 53 40074.5 54 35766.5 55 30976.5 56 26098.0 57 21402.5 58 17314.5 59 13711.5 60 10489.0 61 7817.0 62 5752.0 63 4078.0 64 2782.5 65 1856.0 66 1175.0 67 709.5 68 436.5 69 295.5 70 200.5 71 130.5 72 93.0 73 73.5 74 46.0 75 32.5 76 22.0 77 12.5 78 11.0 79 7.0 80 4.0 81 2.5 82 0.5 83 1.5 84 2.5 85 1.5 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.990236326643632 2 29.001430640999402 3 22.80553114302798 4 29.08630597658805 5 32.18340295919964 6 24.83852617892443 7 23.77547764949496 8 19.795968120583172 9 11.679688910826721 10-11 15.30334404809201 12-13 15.392859436010417 14-15 19.51736432988615 16-17 22.803750366187913 18-19 26.746590941012645 20-21 28.793330564350754 22-23 28.401810473174983 24-25 26.929684897808492 26-27 28.367323597751103 28-29 25.347326727905322 30-31 27.208464258053123 32-33 26.21707318541348 34-35 24.2817198993535 36-37 27.22531893462392 38-39 27.355616620316308 40-41 24.422401269718968 42-43 25.275443538839998 44-45 24.00131025045247 46-47 27.257999951843782 48-49 22.66414749447207 50-51 28.29468796776744 52-53 21.853542893145363 54-55 22.997278170384728 56-57 25.08788509926201 58-59 24.360450300775717 60-61 23.91385153437752 62-63 23.739184915867074 64-65 22.301470971832625 66-67 22.78554129596411 68-69 23.776731717692194 70-71 22.278320872911724 72-73 22.130967859736987 74-75 19.471992142510302 76-77 21.682111770583774 78-79 20.811487465337557 80-81 21.825802904622595 82-83 22.68060086921975 84-85 19.645555181007186 86-87 21.1907427695444 88-89 20.04527687819286 90-91 21.17667212437146 92-93 21.155327883654575 94-95 20.229048031815207 96-97 19.122683986853353 98-99 18.506209142458136 100-101 20.199376778268704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1993512.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.79849282900116 #Duplication Level Percentage of deduplicated Percentage of total 1 91.26385223428701 74.65245562533347 2 6.053654830190814 9.903596824332231 3 1.3485076431606884 3.309176783367985 4 0.5218864930179308 1.7075811422671912 5 0.26000666665754557 1.0634076729039867 6 0.16004931556784632 0.7855075675057813 7 0.09301304485082496 0.5325828817563638 8 0.06462970482786319 0.42292899575219434 9 0.042820278085041 0.3152370788887555 >10 0.18560394772840325 2.3696306920796006 >50 0.004150728500201514 0.22176353574298446 >100 0.0015820829389966242 0.18077168702721955 >500 6.075754672205641E-5 0.03404364888648506 >1k 1.2151509344411282E-4 0.13557821045595803 >5k 0.0 0.0 >10k+ 6.075754672205641E-5 4.365737653699845 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 87844 4.40649466870528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.003254557785456E-4 0.0 0.0 0.0 0.0 7 1.003254557785456E-4 0.0 0.0 5.01627278892728E-5 0.0 8 1.003254557785456E-4 0.0 0.0 5.01627278892728E-5 0.0 9 1.003254557785456E-4 0.0 0.0 5.01627278892728E-5 0.0 10-11 1.003254557785456E-4 0.0 0.0 5.01627278892728E-5 0.0 12-13 1.003254557785456E-4 0.0 0.0 5.01627278892728E-5 0.0 14-15 1.003254557785456E-4 0.0 0.0 5.01627278892728E-5 0.0 16-17 1.003254557785456E-4 0.0 0.0 5.01627278892728E-5 5.01627278892728E-5 18-19 1.003254557785456E-4 0.0 0.0 7.52440918339092E-5 5.01627278892728E-5 20-21 1.504881836678184E-4 0.0 0.0 1.003254557785456E-4 5.01627278892728E-5 22-23 1.504881836678184E-4 0.0 0.0 1.504881836678184E-4 5.01627278892728E-5 24-25 1.504881836678184E-4 0.0 0.0 1.504881836678184E-4 5.01627278892728E-5 26-27 1.755695476124548E-4 0.0 0.0 2.006509115570912E-4 5.01627278892728E-5 28-29 2.006509115570912E-4 0.0 0.0 5.01627278892728E-4 5.01627278892728E-5 30-31 2.006509115570912E-4 0.0 0.0 0.0014547191087889112 5.01627278892728E-5 32-33 2.006509115570912E-4 0.0 0.0 0.0034612282243598235 5.01627278892728E-5 34-35 2.2573227550172758E-4 0.0 0.0 0.006496073261660827 5.01627278892728E-5 36-37 2.50813639446364E-4 0.0 0.0 0.011412020594809561 5.01627278892728E-5 38-39 2.50813639446364E-4 0.0 0.0 0.019814277516262756 5.01627278892728E-5 40-41 2.50813639446364E-4 0.0 0.0 0.02916962626761213 5.01627278892728E-5 42-43 2.50813639446364E-4 5.01627278892728E-5 0.0 0.03782269682851169 5.01627278892728E-5 44-45 2.50813639446364E-4 5.01627278892728E-5 0.0 0.04863276468864998 5.01627278892728E-5 46-47 2.50813639446364E-4 5.01627278892728E-5 0.0 0.06247767758608927 5.01627278892728E-5 48-49 2.50813639446364E-4 5.01627278892728E-5 0.0 0.07925711006505103 5.01627278892728E-5 50-51 2.50813639446364E-4 5.01627278892728E-5 0.0 0.09794272620380515 5.01627278892728E-5 52-53 3.009763673356368E-4 5.01627278892728E-5 0.0 0.11860977009418554 5.01627278892728E-5 54-55 3.009763673356368E-4 5.01627278892728E-5 0.0 0.14196051992664205 5.01627278892728E-5 56-57 3.009763673356368E-4 5.01627278892728E-5 0.0 0.1699262407249116 5.01627278892728E-5 58-59 3.009763673356368E-4 5.01627278892728E-5 0.0 0.19927143654013618 5.01627278892728E-5 60-61 3.009763673356368E-4 5.01627278892728E-5 0.0 0.23012151419203897 5.01627278892728E-5 62-63 3.009763673356368E-4 5.01627278892728E-5 0.0 0.2634295655105161 5.01627278892728E-5 64-65 3.009763673356368E-4 5.01627278892728E-5 0.0 0.29934607867923546 5.01627278892728E-5 66-67 3.009763673356368E-4 5.01627278892728E-5 0.0 0.3405798410042177 5.01627278892728E-5 68-69 3.009763673356368E-4 5.01627278892728E-5 0.0 0.3876826424922448 5.01627278892728E-5 70-71 3.009763673356368E-4 5.01627278892728E-5 0.0 0.4412062731500989 1.003254557785456E-4 72-73 3.009763673356368E-4 5.01627278892728E-5 0.0 0.4978149115731433 1.504881836678184E-4 74-75 3.009763673356368E-4 5.01627278892728E-5 0.0 0.554398468632243 1.504881836678184E-4 76-77 3.009763673356368E-4 5.01627278892728E-5 0.0 0.617101878493834 1.504881836678184E-4 78-79 3.009763673356368E-4 5.01627278892728E-5 0.0 0.6883329520966013 1.504881836678184E-4 80-81 3.009763673356368E-4 7.52440918339092E-5 0.0 0.7680666080766005 1.504881836678184E-4 82-83 3.009763673356368E-4 1.003254557785456E-4 0.0 0.8518885263795752 1.504881836678184E-4 84-85 3.009763673356368E-4 1.003254557785456E-4 0.0 0.9345316205771523 1.504881836678184E-4 86-87 3.009763673356368E-4 1.003254557785456E-4 0.0 1.0218900111963207 1.504881836678184E-4 88-89 3.009763673356368E-4 1.003254557785456E-4 0.0 1.1219646533354202 1.504881836678184E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1585 0.0 54.893703 1 GTATCAA 2925 0.0 42.169075 1 ATCAACG 3410 0.0 36.009335 3 TCAACGC 3485 0.0 35.370834 4 TATCAAC 3595 0.0 33.59436 2 CAACGCA 3630 0.0 33.53334 5 AACGCAG 3875 0.0 31.745628 6 ACGCAGA 4425 0.0 27.32569 7 CGCAGAG 4545 0.0 26.561176 8 GTACATG 2815 0.0 24.544731 1 GTGGTAT 720 0.0 24.16848 1 GCAGAGT 5210 0.0 23.097338 9 TACATGG 3005 0.0 21.627806 2 GTATAGG 430 0.0 21.424316 1 ACATGGG 3100 0.0 20.616821 3 AGAGTAC 4470 0.0 20.18478 10-11 TGGTATC 875 0.0 19.884932 2 GAGTACT 3395 0.0 19.864653 12-13 CAGAGTA 4845 0.0 19.851387 10-11 TAACGCA 230 1.186545E-6 19.687775 4 >>END_MODULE