FastQCFastQC Report
Sat 18 Jun 2016
SRR1930981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993512
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49630.2489576185144609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44850.22497983458338852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.13363350709702274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.051.574161
GTATCAA49200.040.0801851
TCAACGC60050.032.12224
ATCAACG60500.031.8856753
CAACGCA60800.031.6081185
TATCAAC63200.030.825742
AACGCAG64100.030.2021926
ACGCAGA73750.026.0572937
CGCAGAG74700.025.5353498
GTGGTAT13850.024.0156881
GTACATG49700.022.1808591
GCAGAGT86350.021.705029
TACATGG50200.021.4866582
TGGTATC14600.020.8292622
ACATGGG49800.020.7040523
TACGGCC1656.3146515E-520.1560944
AGAGTAC78650.020.14991210-11
GAGTACT61900.019.8917112-13
CAGAGTA82650.019.2321310-11
GTACTTT66900.018.33411214-15
Produced by FastQC (version 0.11.5)