FastQCFastQC Report
Sat 18 Jun 2016
SRR1930980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314136
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN597634.547702825278358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.067.778371
GTATCAA22550.044.8805431
ATCAACG27350.035.6302033
TCAACGC28050.035.279014
TATCAAC28350.034.2408872
CAACGCA29050.033.8770835
AACGCAG31400.030.8599556
TGGTATC5000.029.7895722
ACGCAGA36650.025.5423937
CGCAGAG36950.025.1709378
GTGGTAT6150.025.06021
GTACATG21800.024.7440721
GAGTACT26050.023.1445112-13
TACATGG21250.022.7198352
GCAGAGT39700.022.6469979
ACATGGG21400.022.1785133
CGCGCCC500.002752340621.7577994-95
CAGAGTA37350.020.83045610-11
AGAGTAC34500.020.09712810-11
GTACTAG2851.736953E-819.8283351
Produced by FastQC (version 0.11.5)