##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930980_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1314136 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.089184833228828 28.0 2.0 31.0 2.0 33.0 2 19.747734633249525 27.0 2.0 31.0 2.0 33.0 3 20.421456378944036 27.0 2.0 31.0 2.0 33.0 4 21.892349802455758 30.0 2.0 35.0 2.0 37.0 5 21.22072220835591 30.0 2.0 35.0 2.0 37.0 6 22.24660461322116 30.0 2.0 35.0 2.0 37.0 7 22.47578789409924 30.0 2.0 35.0 2.0 37.0 8 22.9363330735936 32.0 2.0 35.0 2.0 37.0 9 24.304363475317622 32.0 2.0 37.0 2.0 39.0 10-11 24.69844939945333 32.5 2.0 37.0 2.0 39.0 12-13 24.604678282917448 32.5 2.0 37.0 2.0 39.0 14-15 24.548556998666804 32.5 2.0 38.0 2.0 40.0 16-17 23.326549535207924 31.5 2.0 38.0 2.0 40.0 18-19 22.22406546963176 29.5 2.0 38.0 2.0 40.0 20-21 21.878756080040425 28.0 2.0 37.5 2.0 40.0 22-23 21.51872370896163 27.0 2.0 37.0 2.0 40.0 24-25 21.354819820779582 27.0 2.0 37.0 2.0 40.0 26-27 21.07190579970414 27.0 2.0 37.0 2.0 39.0 28-29 20.886223724180752 26.5 2.0 37.0 2.0 39.0 30-31 20.880960950769172 26.0 2.0 37.0 2.0 39.0 32-33 20.74150696731541 26.0 2.0 36.5 2.0 39.0 34-35 20.414301487821653 25.0 2.0 36.0 2.0 39.0 36-37 19.928752807928554 24.0 2.0 36.0 2.0 39.0 38-39 19.820288767677013 24.0 2.0 36.0 2.0 39.0 40-41 19.703279188759762 24.0 2.0 35.0 2.0 39.0 42-43 19.56768819969927 24.0 2.0 35.0 2.0 39.0 44-45 19.180978985432255 23.0 2.0 35.0 2.0 38.5 46-47 18.838017526344306 20.0 2.0 35.0 2.0 38.0 48-49 18.60255825881035 18.5 2.0 35.0 2.0 38.0 50-51 16.36691788368936 11.0 2.0 33.0 2.0 37.5 52-53 18.274461319071996 19.5 2.0 34.0 2.0 38.0 54-55 19.032586809888777 22.0 2.0 35.5 2.0 39.0 56-57 19.022583279051787 22.0 2.0 36.0 2.0 39.0 58-59 18.75128905988421 21.0 2.0 35.5 2.0 39.0 60-61 18.467486241911033 19.0 2.0 35.0 2.0 39.0 62-63 18.033969467391504 16.0 2.0 34.5 2.0 39.0 64-65 17.691320761321506 13.0 2.0 34.0 2.0 38.0 66-67 17.346217210395274 9.5 2.0 34.0 2.0 38.0 68-69 16.910894686699095 8.0 2.0 34.0 2.0 38.0 70-71 16.568415673872416 7.0 2.0 33.0 2.0 37.0 72-73 16.173810777575532 4.0 2.0 32.5 2.0 36.5 74-75 15.73671750869012 2.0 2.0 32.0 2.0 36.0 76-77 15.326277112871118 2.0 2.0 31.5 2.0 36.0 78-79 14.956659736891767 2.0 2.0 31.0 2.0 35.0 80-81 14.532363849708098 2.0 2.0 31.0 2.0 35.0 82-83 14.077124818131457 2.0 2.0 30.5 2.0 34.0 84-85 13.687297585638017 2.0 2.0 30.0 2.0 34.0 86-87 13.205839806534483 2.0 2.0 29.5 2.0 34.0 88-89 12.790384709040769 2.0 2.0 29.0 2.0 34.0 90-91 12.312686814758898 2.0 2.0 29.0 2.0 34.0 92-93 11.77161952796362 2.0 2.0 28.0 2.0 34.0 94-95 11.20908262158559 2.0 2.0 26.5 2.0 34.0 96-97 10.563231278954387 2.0 2.0 25.5 2.0 33.5 98-99 9.883731211990234 2.0 2.0 24.0 2.0 33.0 100-101 8.94174765777667 2.0 2.0 10.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.568950544562249 1101 2 9.447634311673436 1101 3 8.675466264870394 1101 4 10.703731061791466 1101 5 11.565324925090025 1101 6 10.60978162849776 1101 7 10.310097386527392 1101 8 9.82632267607811 1101 9 9.568666075333102 1101 10-11 9.035181890348664 1101 12-13 8.867712617747088 1101 14-15 9.77753433414858 1101 16-17 10.77530033862579 1101 18-19 11.49237014950738 1101 20-21 11.70063840199236 1101 22-23 11.652513989223838 1101 24-25 11.566597223402116 1101 26-27 11.812293916140856 1101 28-29 11.729193029514224 1101 30-31 11.291008821778096 1101 32-33 11.507924503077945 1101 34-35 10.917423077278034 1101 36-37 11.560604674119435 1101 38-39 11.039684527317295 1101 40-41 10.70855792055853 1101 42-43 10.396922143000804 1101 44-45 10.478096022578004 1101 46-47 10.341098211729324 1101 48-49 10.463059873051439 1101 50-51 12.045180263886937 1101 52-53 11.244751396849729 1101 54-55 10.829889018983959 1101 56-57 11.080033486673862 1101 58-59 11.02295254329633 1101 60-61 10.613714665760433 1101 62-63 10.670072810678807 1101 64-65 10.404494982199523 1101 66-67 10.00872240987092 1101 68-69 9.97875537062216 1101 70-71 9.70573308323581 1101 72-73 9.715608950916472 1101 74-75 9.477836571795253 1101 76-77 9.372086669382503 1101 78-79 9.015727016855697 1101 80-81 8.647435006723043 1101 82-83 8.46332214412582 1101 84-85 7.972680592171352 1101 86-87 7.745838922438887 1101 88-89 7.438237274121521 1101 90-91 7.072616037730631 1101 92-93 6.828032365635618 1101 94-95 6.496825345266389 1101 96-97 6.1741640689083575 1101 98-99 5.690078556807007 1101 100-101 5.039113340255071 1102 1 7.664935149596687 1102 2 8.95073743768016 1102 3 8.442359575846648 1102 4 10.3793456822277 1102 5 11.112816071618816 1102 6 10.104788282119006 1102 7 9.797918243040254 1102 8 9.087084296579228 1102 9 8.71538212336667 1102 10-11 7.819148086082375 1102 12-13 8.154526381237886 1102 14-15 8.76594826793566 1102 16-17 9.425996606792339 1102 18-19 10.77333253975678 1102 20-21 10.895856446112301 1102 22-23 11.218928114252723 1102 24-25 10.760092099146167 1102 26-27 11.111702603299992 1102 28-29 10.755325710348721 1102 30-31 10.6959755878222 1102 32-33 10.703370785189207 1102 34-35 10.221396239677617 1102 36-37 10.613935241276614 1102 38-39 10.408081135507228 1102 40-41 10.475993367910686 1102 42-43 9.77798652888124 1102 44-45 9.605322765923528 1102 46-47 9.362448993600967 1102 48-49 9.204029673920903 1102 50-51 11.0580720107784 1102 52-53 10.0340888692022 1102 54-55 9.810029752464597 1102 56-57 9.710517032479121 1102 58-59 9.661517193841767 1102 60-61 9.717136072776505 1102 62-63 9.830390932319979 1102 64-65 9.520974618931152 1102 66-67 9.177677148171306 1102 68-69 9.022979185008325 1102 70-71 8.682078008327075 1102 72-73 8.548313918308605 1102 74-75 8.194414789125632 1102 76-77 7.914609228516634 1102 78-79 7.940548496042785 1102 80-81 7.188186017857184 1102 82-83 7.0126870050372485 1102 84-85 6.982953578469102 1102 86-87 6.941584589047496 1102 88-89 6.763038149280463 1102 90-91 6.691590640135496 1102 92-93 6.447756567627948 1102 94-95 6.006188510045112 1102 96-97 5.946301731136618 1102 98-99 5.627374579774239 1102 100-101 4.906547763097539 1103 1 7.801574645245175 1103 2 9.220306345871723 1103 3 8.479173333823859 1103 4 10.671778391064954 1103 5 11.457630085270488 1103 6 10.453541140234261 1103 7 10.07955431287527 1103 8 9.560519383861777 1103 9 9.33891437815748 1103 10-11 8.656091139082125 1103 12-13 8.829231654747886 1103 14-15 9.517851809139056 1103 16-17 10.604873130606506 1103 18-19 11.191537738695054 1103 20-21 11.659484006921883 1103 22-23 11.699329769856991 1103 24-25 11.634576124538679 1103 26-27 11.6092156645643 1103 28-29 11.247194589720838 1103 30-31 10.803784704410873 1103 32-33 11.135980941072134 1103 34-35 11.361281567439939 1103 36-37 11.441382455153907 1103 38-39 11.402999605632 1103 40-41 10.968663753070803 1103 42-43 10.867242706839637 1103 44-45 10.590847442433663 1103 46-47 11.010408812341232 1103 48-49 10.685646343895641 1103 50-51 12.579634450854702 1103 52-53 11.739544883663505 1103 54-55 11.500225477991336 1103 56-57 10.89914703228849 1103 58-59 10.725746711009524 1103 60-61 10.848326226169966 1103 62-63 10.634917996246443 1103 64-65 10.50913930243869 1103 66-67 10.485549846961 1103 68-69 10.187502335321362 1103 70-71 9.883745443138498 1103 72-73 9.68343927168323 1103 74-75 9.3315356367986 1103 76-77 8.96946261379225 1103 78-79 8.530018577084858 1103 80-81 8.534241678635489 1103 82-83 8.435231661160705 1103 84-85 7.748820253209702 1103 86-87 7.447661342968681 1103 88-89 7.55759082550801 1103 90-91 7.288854587009036 1103 92-93 6.9237071888159605 1103 94-95 6.38093998437842 1103 96-97 6.33899471208775 1103 98-99 5.6852699864928695 1103 100-101 5.1489607917297135 1104 1 8.074337663486386 1104 2 9.523079097891344 1104 3 8.830409853640624 1104 4 10.896607225046289 1104 5 11.598041763604854 1104 6 10.833978445352468 1104 7 10.518885926757722 1104 8 9.745165310797947 1104 9 9.709891770494739 1104 10-11 8.81276812612025 1104 12-13 8.682503469135185 1104 14-15 9.171563843278772 1104 16-17 10.08511438532905 1104 18-19 11.191017229019025 1104 20-21 11.666023511214103 1104 22-23 11.59486762985556 1104 24-25 11.60630252287569 1104 26-27 11.660281980741626 1104 28-29 11.435160450005373 1104 30-31 11.325023313965378 1104 32-33 11.407891566288669 1104 34-35 11.364534409208296 1104 36-37 11.308303006624698 1104 38-39 11.076982370794976 1104 40-41 10.906124226781348 1104 42-43 10.304601718283703 1104 44-45 10.627203379763529 1104 46-47 10.518629180685004 1104 48-49 10.579054871125475 1104 50-51 12.651336652228427 1104 52-53 11.638106539175737 1104 54-55 11.080129625020884 1104 56-57 10.736556087452065 1104 58-59 10.61125300496963 1104 60-61 10.661869926435585 1104 62-63 10.480834208761774 1104 64-65 10.484246951428744 1104 66-67 10.438437534556204 1104 68-69 9.863628731458647 1104 70-71 9.66899711905317 1104 72-73 9.796783621967268 1104 74-75 9.435767080752386 1104 76-77 8.98968412599247 1104 78-79 8.73622207354605 1104 80-81 8.285834278764174 1104 82-83 8.161845571115004 1104 84-85 8.166042991756013 1104 86-87 7.674285701611668 1104 88-89 7.488188985438937 1104 90-91 7.390525277337398 1104 92-93 6.962111894025455 1104 94-95 6.345917629683937 1104 96-97 6.185776241005872 1104 98-99 5.880061865112102 1104 100-101 5.135430839738689 1105 1 7.9022833285639535 1105 2 9.21382613608068 1105 3 8.856716038271095 1105 4 10.612648032513185 1105 5 10.986205240010861 1105 6 10.604439927745066 1105 7 9.996503186590456 1105 8 9.423311020359623 1105 9 9.595972801239693 1105 10-11 8.919506932070394 1105 12-13 8.626417052061853 1105 14-15 9.323298053431333 1105 16-17 10.348403743712822 1105 18-19 10.94137377180013 1105 20-21 11.184294687389912 1105 22-23 11.626478142039108 1105 24-25 11.659553748198395 1105 26-27 11.689955290546617 1105 28-29 11.340892200133329 1105 30-31 11.011523995965657 1105 32-33 11.005812872299114 1105 34-35 11.150581942886244 1105 36-37 11.054711719077837 1105 38-39 10.811699266983545 1105 40-41 10.49590038598085 1105 42-43 10.09470212909173 1105 44-45 10.503320506947873 1105 46-47 10.538331887515998 1105 48-49 10.04254815965313 1105 50-51 11.776046768610637 1105 52-53 10.586915785438137 1105 54-55 10.566364474297483 1105 56-57 10.728046224575657 1105 58-59 10.594783970998243 1105 60-61 10.241589730169572 1105 62-63 10.519481784780016 1105 64-65 10.392105631322732 1105 66-67 10.09485651051067 1105 68-69 9.828432693223753 1105 70-71 9.701524378966294 1105 72-73 9.493860801889394 1105 74-75 9.232612460970893 1105 76-77 8.905667586480673 1105 78-79 8.601506249263627 1105 80-81 8.054758915722964 1105 82-83 8.137129023295932 1105 84-85 7.92717835474363 1105 86-87 7.838486448194297 1105 88-89 7.520644159663883 1105 90-91 6.969332352989854 1105 92-93 6.665696080194113 1105 94-95 6.302707753140728 1105 96-97 5.959608868221036 1105 98-99 5.903498289018826 1105 100-101 5.377905656643719 1106 1 8.037167485332994 1106 2 9.130845369102442 1106 3 8.858332455447286 1106 4 10.72493983095299 1106 5 11.217854219793935 1106 6 10.544862070317752 1106 7 9.738865029774562 1106 8 9.20454184004711 1106 9 8.832255510261174 1106 10-11 8.753210401510746 1106 12-13 8.241016344586512 1106 14-15 8.834467730382162 1106 16-17 10.177000271929842 1106 18-19 11.340207140272973 1106 20-21 11.379949102082914 1106 22-23 11.714551794439135 1106 24-25 11.106565719056032 1106 26-27 11.543449328544131 1106 28-29 11.351703454145085 1106 30-31 11.297374489090029 1106 32-33 11.289758804315888 1106 34-35 11.047281172159796 1106 36-37 10.97181528690879 1106 38-39 10.576011586893589 1106 40-41 10.908075052741221 1106 42-43 10.640826241819259 1106 44-45 10.016565757258274 1106 46-47 9.970835928558937 1106 48-49 10.17961565092682 1106 50-51 11.599529327703568 1106 52-53 10.969498304706299 1106 54-55 10.377386565332223 1106 56-57 10.248197558705485 1106 58-59 10.312161626959078 1106 60-61 10.156688003648615 1106 62-63 10.131525264059423 1106 64-65 9.8427955369146 1106 66-67 9.734936628579405 1106 68-69 9.339888171472403 1106 70-71 9.20036243270631 1106 72-73 8.863385481534248 1106 74-75 8.493481109505575 1106 76-77 8.139973112753308 1106 78-79 8.151303611458205 1106 80-81 7.644008570184507 1106 82-83 7.5279131027580615 1106 84-85 7.226920665048874 1106 86-87 7.3890193973357245 1106 88-89 7.124638349932583 1106 90-91 6.706774665637733 1106 92-93 6.362060312864479 1106 94-95 6.125845945096122 1106 96-97 5.595769760295905 1106 98-99 5.371455746792854 1106 100-101 4.626812818074601 1107 1 7.205339222435118 1107 2 8.53333036683362 1107 3 8.334367063909738 1107 4 10.167245974108031 1107 5 10.945402311179038 1107 6 9.821279574409278 1107 7 9.754455153004752 1107 8 8.020935993113593 1107 9 5.5344040085047475 1107 10-11 7.277851086338185 1107 12-13 7.439622930799214 1107 14-15 8.023071614402003 1107 16-17 9.13575843586628 1107 18-19 10.532048812165577 1107 20-21 11.054007795751115 1107 22-23 10.177885586635409 1107 24-25 10.547647208015281 1107 26-27 10.609214074614481 1107 28-29 10.583932712265689 1107 30-31 10.373448355826035 1107 32-33 10.55413880400998 1107 34-35 10.773940853912858 1107 36-37 11.038567607990483 1107 38-39 10.688112057483021 1107 40-41 10.490606650552959 1107 42-43 10.009450468762669 1107 44-45 9.473125967723405 1107 46-47 9.829364406102695 1107 48-49 9.59000609230198 1107 50-51 11.112461614829403 1107 52-53 10.15503163090176 1107 54-55 10.091471108279244 1107 56-57 9.549771458581592 1107 58-59 9.747582021887109 1107 60-61 9.667316798827493 1107 62-63 9.85232985472819 1107 64-65 10.040424745070123 1107 66-67 9.80245652538623 1107 68-69 9.34524284703081 1107 70-71 9.366294153901187 1107 72-73 8.837215508505192 1107 74-75 8.904927190561162 1107 76-77 8.850500448238195 1107 78-79 8.47593537395075 1107 80-81 8.078371795888991 1107 82-83 7.863585872301803 1107 84-85 7.86338726747473 1107 86-87 7.412803283089049 1107 88-89 7.668268822510422 1107 90-91 7.493958113941725 1107 92-93 6.9435100138388 1107 94-95 6.537240531533156 1107 96-97 5.933446741181715 1107 98-99 5.804941269667172 1107 100-101 5.281890136479593 1108 1 6.707281864036286 1108 2 7.9312196239019634 1108 3 7.807034632198331 1108 4 9.072161598982497 1108 5 11.154062722800688 1108 6 9.263445300484452 1108 7 8.694241503629932 1108 8 6.122855149970057 1108 9 7.240451742278385 1108 10-11 6.210683428951771 1108 12-13 4.75794267971845 1108 14-15 6.909118585951468 1108 16-17 8.239326932523305 1108 18-19 7.877912922016499 1108 20-21 8.141079289288175 1108 22-23 9.130438677093906 1108 24-25 8.991250859687074 1108 26-27 7.52129718176981 1108 28-29 9.601344641897978 1108 30-31 9.848922248103158 1108 32-33 10.124262397629131 1108 34-35 9.933492140784164 1108 36-37 9.458422311583135 1108 38-39 9.178698331473772 1108 40-41 9.489501425506273 1108 42-43 8.696977957759348 1108 44-45 8.046522232618965 1108 46-47 8.96436666702439 1108 48-49 9.514542551853115 1108 50-51 10.961219858953324 1108 52-53 10.381219850830217 1108 54-55 9.651065708024003 1108 56-57 9.546915178008938 1108 58-59 9.621172550942788 1108 60-61 9.321573975322561 1108 62-63 9.510840808610535 1108 64-65 9.429713229407959 1108 66-67 8.718705857152539 1108 68-69 9.171538396362678 1108 70-71 9.265387776659963 1108 72-73 8.824456493916841 1108 74-75 8.837836013099926 1108 76-77 8.543467282216502 1108 78-79 8.411783394691488 1108 80-81 8.199501576914347 1108 82-83 7.881781656790126 1108 84-85 7.66989949447745 1108 86-87 7.31205527336966 1108 88-89 6.693638269475587 1108 90-91 6.68587946476776 1108 92-93 6.452393015518783 1108 94-95 6.283281858503633 1108 96-97 6.159897619439061 1108 98-99 5.641699375766716 1108 100-101 5.0823429388379555 1109 1 6.714713252794688 1109 2 8.051859817709872 1109 3 8.048403226208908 1109 4 8.921179943861294 1109 5 10.39934111654173 1109 6 9.364713421257466 1109 7 7.867994254951487 1109 8 8.3970124199206 1109 9 7.7255566566053595 1109 10-11 6.022555086596082 1109 12-13 6.579903456591143 1109 14-15 7.760556452751604 1109 16-17 6.682906635206216 1109 18-19 5.829319916578477 1109 20-21 8.216728067834737 1109 22-23 9.531388280472829 1109 24-25 9.047315767285749 1109 26-27 9.560601592294677 1109 28-29 10.045737946006962 1109 30-31 7.4710042574858555 1109 32-33 6.570837271524756 1109 34-35 8.137650713317512 1109 36-37 6.620637974167007 1109 38-39 7.116922316228422 1109 40-41 8.035242851537003 1109 42-43 6.913222344858237 1109 44-45 7.584792782144806 1109 46-47 7.272023648765664 1109 48-49 7.888838695592614 1109 50-51 9.517962725004736 1109 52-53 9.88165498528343 1109 54-55 9.833190630843408 1109 56-57 8.778954663704113 1109 58-59 8.85487462461359 1109 60-61 9.35260929275276 1109 62-63 9.655873147536429 1109 64-65 9.742276179029801 1109 66-67 9.6523561864906 1109 68-69 9.036030809587075 1109 70-71 8.367572064434352 1109 72-73 7.617859168416782 1109 74-75 6.996242133803198 1109 76-77 6.995885060523063 1109 78-79 7.722211954691323 1109 80-81 7.677658442756524 1109 82-83 7.367615043115675 1109 84-85 7.268082442745426 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-15.963815237732693 2313 64-65 -15.623871129752068 2313 66-67 -15.271156561384753 2313 68-69 -14.83932329806165 2313 70-71 -14.499637567293693 2313 72-73 -14.117976525634209 2313 74-75 -13.688956166480088 2313 76-77 -13.268987460723396 2313 78-79 -12.9003092917676 2313 80-81 -12.472837307951693 2313 82-83 -12.021549262833334 2313 84-85 -11.648348152155426 2313 86-87 -11.161876660011947 2313 88-89 -10.753139427845197 2313 90-91 -10.267777305688432 2313 92-93 -9.727186998963475 2313 94-95 -9.154082370316063 2313 96-97 -8.515699773754275 2313 98-99 -7.857576511271661 2313 100-101 -6.915122665796368 2314 1 -18.921972299613245 2314 2 -17.60607219362157 2314 3 -18.295789408351997 2314 4 -19.740293143957402 2314 5 -19.060998826801047 2314 6 -20.123955133983326 2314 7 -20.359342855530098 2314 8 -20.809078231637475 2314 9 -22.147672805151014 2314 10-11 -22.55180751963621 2314 12-13 -22.464359999499507 2314 14-15 -22.4221630939906 2314 16-17 -21.185365319468005 2314 18-19 -20.09348461599943 2314 20-21 -19.735821507236086 2314 22-23 -19.381147130292046 2314 24-25 -19.197018510334647 2314 26-27 -18.91425676464583 2314 28-29 -18.737902280621935 2314 30-31 -18.7517294535623 2314 32-33 -18.59948850751207 2314 34-35 -18.299672232858125 2314 36-37 -17.814206218467554 2314 38-39 -17.73187371776591 2314 40-41 -17.588340717868096 2314 42-43 -17.469568023413718 2314 44-45 -17.08128370510732 2314 46-47 -16.748160487211674 2314 48-49 -16.50138793330257 2314 50-51 -14.292943790576 2314 52-53 -16.179605637946032 2314 54-55 -16.940534581621183 2314 56-57 -16.932447602584837 2314 58-59 -16.66955880314845 2314 60-61 -16.393849630759988 2314 62-63 -15.963815237732693 2314 64-65 -15.623871129752068 2314 66-67 -15.271156561384753 2314 68-69 -14.83932329806165 2314 70-71 -14.499637567293693 2314 72-73 -14.117976525634209 2314 74-75 -13.688956166480088 2314 76-77 -13.268987460723396 2314 78-79 -12.9003092917676 2314 80-81 -12.472837307951693 2314 82-83 -12.021549262833334 2314 84-85 -11.648348152155426 2314 86-87 -11.161876660011947 2314 88-89 -10.753139427845197 2314 90-91 -10.267777305688432 2314 92-93 -9.727186998963475 2314 94-95 -9.154082370316063 2314 96-97 -8.515699773754275 2314 98-99 -7.857576511271661 2314 100-101 -6.915122665796368 2315 1 -18.921972299613245 2315 2 -17.60607219362157 2315 3 -18.295789408351997 2315 4 -19.740293143957402 2315 5 -19.060998826801047 2315 6 -20.123955133983326 2315 7 -20.359342855530098 2315 8 -20.809078231637475 2315 9 -22.147672805151014 2315 10-11 -22.55180751963621 2315 12-13 -22.464359999499507 2315 14-15 -22.4221630939906 2315 16-17 -21.185365319468005 2315 18-19 -20.09348461599943 2315 20-21 -19.735821507236086 2315 22-23 -19.381147130292046 2315 24-25 -19.197018510334647 2315 26-27 -18.91425676464583 2315 28-29 -18.737902280621935 2315 30-31 -18.7517294535623 2315 32-33 -18.59948850751207 2315 34-35 -18.299672232858125 2315 36-37 -17.814206218467554 2315 38-39 -17.73187371776591 2315 40-41 -17.588340717868096 2315 42-43 -17.469568023413718 2315 44-45 -17.08128370510732 2315 46-47 -16.748160487211674 2315 48-49 -16.50138793330257 2315 50-51 -14.292943790576 2315 52-53 -16.179605637946032 2315 54-55 -16.940534581621183 2315 56-57 -16.932447602584837 2315 58-59 -16.66955880314845 2315 60-61 -16.393849630759988 2315 62-63 -15.963815237732693 2315 64-65 -15.623871129752068 2315 66-67 -15.271156561384753 2315 68-69 -14.83932329806165 2315 70-71 -14.499637567293693 2315 72-73 -14.117976525634209 2315 74-75 -13.688956166480088 2315 76-77 -13.268987460723396 2315 78-79 -12.9003092917676 2315 80-81 -12.472837307951693 2315 82-83 -12.021549262833334 2315 84-85 -11.648348152155426 2315 86-87 -11.161876660011947 2315 88-89 -10.753139427845197 2315 90-91 -10.267777305688432 2315 92-93 -9.727186998963475 2315 94-95 -9.154082370316063 2315 96-97 -8.515699773754275 2315 98-99 -7.857576511271661 2315 100-101 -6.915122665796368 2316 1 -18.921972299613245 2316 2 -17.60607219362157 2316 3 -18.295789408351997 2316 4 -19.740293143957402 2316 5 -19.060998826801047 2316 6 -20.123955133983326 2316 7 -20.359342855530098 2316 8 -20.809078231637475 2316 9 -22.147672805151014 2316 10-11 -22.55180751963621 2316 12-13 -22.464359999499507 2316 14-15 -22.4221630939906 2316 16-17 -21.185365319468005 2316 18-19 -20.09348461599943 2316 20-21 -19.735821507236086 2316 22-23 -19.381147130292046 2316 24-25 -19.197018510334647 2316 26-27 -18.91425676464583 2316 28-29 -18.737902280621935 2316 30-31 -18.7517294535623 2316 32-33 -18.59948850751207 2316 34-35 -18.299672232858125 2316 36-37 -17.814206218467554 2316 38-39 -17.73187371776591 2316 40-41 -17.588340717868096 2316 42-43 -17.469568023413718 2316 44-45 -17.08128370510732 2316 46-47 -16.748160487211674 2316 48-49 -16.50138793330257 2316 50-51 -14.292943790576 2316 52-53 -16.179605637946032 2316 54-55 -16.940534581621183 2316 56-57 -16.932447602584837 2316 58-59 -16.66955880314845 2316 60-61 -16.393849630759988 2316 62-63 -15.963815237732693 2316 64-65 -15.623871129752068 2316 66-67 -15.271156561384753 2316 68-69 -14.83932329806165 2316 70-71 -14.499637567293693 2316 72-73 -14.117976525634209 2316 74-75 -13.688956166480088 2316 76-77 -13.268987460723396 2316 78-79 -12.9003092917676 2316 80-81 -12.472837307951693 2316 82-83 -12.021549262833334 2316 84-85 -11.648348152155426 2316 86-87 -11.161876660011947 2316 88-89 -10.753139427845197 2316 90-91 -10.267777305688432 2316 92-93 -9.727186998963475 2316 94-95 -9.154082370316063 2316 96-97 -8.515699773754275 2316 98-99 -7.857576511271661 2316 100-101 -6.915122665796368 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 336304.0 3 13954.0 4 52362.0 5 27840.0 6 15201.0 7 15347.0 8 14958.0 9 14591.0 10 15218.0 11 16440.0 12 16881.0 13 17495.0 14 17866.0 15 18293.0 16 19378.0 17 19265.0 18 18785.0 19 19316.0 20 20554.0 21 21794.0 22 23174.0 23 24832.0 24 26841.0 25 28274.0 26 30636.0 27 33300.0 28 35152.0 29 38255.0 30 41628.0 31 45397.0 32 49230.0 33 53684.0 34 56308.0 35 54055.0 36 41356.0 37 17834.0 38 2321.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17890911670693 20.807107019127308 14.199553840635973 22.814430023529784 2 14.924691426333428 21.06264124086918 41.96308941448845 22.049577918308938 3 18.535779012566966 26.459939057178705 30.766664675071198 24.237617255183128 4 11.986748416646648 17.64388760935654 40.17712254127805 30.19224143271876 5 12.23803652203812 38.008969406331865 36.410916762588535 13.342077309041475 6 28.8849352425487 39.3267927556824 18.299149533820056 13.48912246794885 7 25.33879043265705 33.42012636608232 23.694397057443805 17.546686143816828 8 23.724034699518405 37.34648602295436 21.136309692436086 17.79316958509115 9 24.89771338953826 17.132509032934472 21.990538163606587 35.97923941392068 10-11 23.493035664939434 27.69411469929911 29.56295445016872 19.249895185592734 12-13 24.219710653119098 25.337335269734705 29.675818712643075 20.76713536450312 14-15 21.507710228480516 27.039848016464248 27.490393790256242 23.962047964798998 16-17 20.658316181905516 29.919041942396092 28.35871412821387 21.063927747484527 18-19 20.4154372254131 28.5494135486354 30.775341912607495 20.259807313344 20-21 21.15056848944234 27.925013535462913 30.833405522468873 20.091012452625883 22-23 20.773635267780264 27.822778543137595 30.558593077320474 20.844993111761667 24-25 20.668629775597793 28.031815637000058 30.799567491042502 20.499987096359646 26-27 20.64257702357737 28.138681182441594 30.57229045325206 20.646451340728973 28-29 20.59167291946668 28.25344865970201 30.618398486135273 20.536479934696033 30-31 20.889457063758815 28.001420202003096 30.59302428947569 20.516098444762395 32-33 20.660868585344325 28.30558194445966 30.39813943879539 20.63541003140062 34-35 20.914038510958367 28.22516833435077 30.454424026840048 20.406369127850816 36-37 20.93375798539133 28.064417916928104 30.26498394317499 20.736840154505572 38-39 20.81198520617201 28.269550501751677 30.377766420385623 20.540697871690686 40-41 21.03627159153519 28.294863309359837 30.303213757533793 20.36565134157118 42-43 20.85282460889595 28.382729537121033 30.032661937618993 20.731783916364023 44-45 20.914788007066093 28.562488997169112 29.930785870240456 20.591937125524343 46-47 21.0703720627565 28.408589704828834 29.618088850507217 20.902949381907444 48-49 20.919818856838166 28.53854559712095 29.60268464780397 20.93895089823692 50-51 21.147173297098497 28.87699159426129 29.20367306348669 20.772162045153518 52-53 20.727253956314616 29.10205546460579 29.2264845704959 20.94420600858369 54-55 20.697634966570202 28.96716894521505 29.392575591258357 20.94262049695639 56-57 20.7402681918401 29.17581921275102 29.24187151262333 20.84204108278555 58-59 20.66028003097557 29.109259062465203 29.313407002859087 20.917053903700136 60-61 20.613399826758616 29.113598437795325 29.37270245438769 20.90029928105837 62-63 20.587415084168224 29.027989508787975 29.35567599078766 21.028919416256134 64-65 20.447983451527293 28.92759252154584 29.462580042953253 21.16184398397361 66-67 20.38918643431745 29.09451791419244 29.148120884901928 21.368174766588176 68-69 20.629809581308876 28.919450971966036 29.226719445020276 21.224020001704805 70-71 20.658817575848627 28.674220559979126 29.287068372486537 21.37989349168571 72-73 20.633357006325575 28.844167387631842 29.176313123112557 21.34616248293003 74-75 20.444539266619266 29.196504300095672 28.89605240787737 21.462904025407685 76-77 20.559197132785282 29.044824267326764 29.011711587083404 21.38426701280455 78-79 20.572031926281007 29.150329268860244 28.847723817502057 21.429914987356696 80-81 20.646633682338315 29.108047422751326 28.61623045138087 21.629088443529486 82-83 20.75436339559465 28.946989068148106 28.861590188772286 21.43705734748496 84-85 20.796212398479838 28.90115025127405 28.73000295279259 21.572634397453527 86-87 20.619432059735164 28.977782587058133 28.737481045180104 21.66530430802661 88-89 20.759517622362914 28.957882002757568 28.654703114727255 21.627897260152267 90-91 20.674824301040385 28.98054901571073 28.792026114562493 21.552600568686394 92-93 20.722851297672136 28.88696276656128 28.60476798102591 21.785417954740673 94-95 20.59187814963764 28.85519014107202 28.59458520862584 21.9583465006645 96-97 20.624934754344174 28.741731595979918 28.58765694545938 22.04567670421653 98-99 20.559289164066854 28.72506546441699 28.34450275541721 22.371142616098947 100-101 20.791884948934694 28.85625060711885 28.23278162860773 22.119082815338732 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 81038.0 1 73799.0 2 49138.5 3 24791.0 4 15381.0 5 12092.5 6 11149.0 7 11429.5 8 11845.5 9 11542.0 10 10569.5 11 9059.0 12 7277.0 13 5569.5 14 4272.0 15 3537.0 16 3359.0 17 3576.5 18 4056.0 19 4701.0 20 5519.0 21 6354.0 22 7185.5 23 8239.5 24 9422.0 25 10776.0 26 12435.0 27 14214.0 28 16146.5 29 18337.5 30 20606.5 31 22945.5 32 25694.0 33 28221.5 34 30334.5 35 32307.5 36 34538.5 37 36599.5 38 37772.5 39 38306.5 40 38364.5 41 38181.0 42 37752.0 43 37567.0 44 37658.5 45 37654.5 46 36835.0 47 35697.5 48 34452.0 49 32717.0 50 30834.0 51 28615.5 52 26164.5 53 23744.5 54 21231.5 55 18358.5 56 15334.0 57 12371.0 58 9806.5 59 7691.0 60 5846.0 61 4359.0 62 3182.5 63 2190.0 64 1425.0 65 920.0 66 578.5 67 361.5 68 221.0 69 134.0 70 91.5 71 59.0 72 37.5 73 26.0 74 17.0 75 10.5 76 7.0 77 4.5 78 3.5 79 3.5 80 3.0 81 2.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.771767914431994 2 29.859542695733165 3 23.58325165736271 4 29.98890525790329 5 33.1637669160574 6 25.66401042205677 7 24.570440197970377 8 20.491029847747875 9 12.177278455197941 10-11 15.874346338582917 12-13 15.929667857816849 14-15 20.171047745438827 16-17 23.559471774610845 18-19 27.63503929578065 20-21 29.725690491699492 22-23 29.298033080290015 24-25 27.75903711640196 26-27 29.292249812804762 28-29 26.169589753267548 30-31 28.094504678359012 32-33 27.068393225663094 34-35 25.08393347416097 36-37 28.142711256673586 38-39 28.234520628001974 40-41 25.21158388477296 42-43 26.098896917822813 44-45 24.788454163039443 46-47 28.141645917926304 48-49 23.43520761930272 50-51 28.98969360857628 52-53 22.60732526922632 54-55 23.744574381951335 56-57 25.865511636542944 58-59 25.170948821126583 60-61 24.713195590106352 62-63 24.55145433958129 64-65 23.042059573742748 66-67 23.553688507125596 68-69 24.56522764767117 70-71 23.010099411324248 72-73 22.90679960065016 74-75 20.105795747167722 76-77 22.439762703403606 78-79 21.516798870132163 80-81 22.56090693809469 82-83 23.457846067682492 84-85 20.368439796185477 86-87 21.94152659998661 88-89 20.77421971546324 90-91 21.8954126513542 92-93 21.907511855698345 94-95 20.898255583896947 96-97 19.81727918571594 98-99 19.22795661940621 100-101 20.8798023948815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1314136.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.7440020531052 #Duplication Level Percentage of deduplicated Percentage of total 1 92.05790544828322 75.25181611969029 2 5.427967751404215 8.8740761402995 3 1.2271952505390158 3.0094755323886675 4 0.46910197119704194 1.5338508998658678 5 0.2440299778889775 0.9973993506787897 6 0.14902429722538943 0.7309105475012876 7 0.09180137399424547 0.5252948192984436 8 0.06538588209959334 0.4275922944474602 9 0.05469164768068108 0.40236427442675704 >10 0.20292999755081317 2.789123970906257 >50 0.007622861594095102 0.4340048520931521 >100 0.001968588602660725 0.2257457763866236 >500 9.37379850245525E-5 0.04160747295883424 >1k 1.87475970049105E-4 0.17738729263296732 >5k 0.0 0.0 >10k+ 9.37379850245525E-5 4.579350656425065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 59763 4.547702825278358 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.804781240297808E-5 0.0 18-19 0.0 0.0 0.0 1.1414343720893424E-4 0.0 20-21 0.0 0.0 0.0 1.521912496119123E-4 0.0 22-23 0.0 0.0 0.0 1.521912496119123E-4 0.0 24-25 0.0 0.0 0.0 1.521912496119123E-4 0.0 26-27 0.0 0.0 0.0 1.9023906201489038E-4 0.0 28-29 0.0 0.0 0.0 3.8047812402978076E-4 0.0 30-31 0.0 0.0 0.0 0.0016360559333280572 0.0 32-33 0.0 0.0 0.0 0.005060359049596085 0.0 34-35 0.0 3.804781240297808E-5 0.0 0.010387052786013016 0.0 36-37 0.0 7.609562480595615E-5 0.0 0.020545818697608163 0.0 38-39 0.0 1.1414343720893424E-4 0.0 0.03686833021848576 0.0 40-41 0.0 1.521912496119123E-4 0.0 0.05284841142773655 0.0 42-43 0.0 1.521912496119123E-4 0.0 0.07206255669124048 0.0 44-45 0.0 1.521912496119123E-4 0.0 0.097212160689609 0.0 46-47 0.0 1.521912496119123E-4 0.0 0.12567192436703659 0.0 48-49 0.0 1.521912496119123E-4 0.0 0.1588115689700305 0.0 50-51 0.0 1.521912496119123E-4 0.0 0.19807691136990388 0.0 52-53 7.609562480595615E-5 1.521912496119123E-4 0.0 0.23943488345194106 0.0 54-55 7.609562480595615E-5 1.521912496119123E-4 0.0 0.2865000273944249 0.0 56-57 7.609562480595615E-5 1.9023906201489038E-4 0.0 0.34558828005624986 0.0 58-59 7.609562480595615E-5 2.2828687441786848E-4 0.0 0.4127046211351032 0.0 60-61 7.609562480595615E-5 2.2828687441786848E-4 0.0 0.4851857037627765 0.0 62-63 7.609562480595615E-5 2.2828687441786848E-4 0.0 0.557096069204405 0.0 64-65 7.609562480595615E-5 2.2828687441786848E-4 0.0 0.6289303390212276 0.0 66-67 7.609562480595615E-5 2.2828687441786848E-4 0.0 0.7153369209883909 0.0 68-69 7.609562480595615E-5 2.2828687441786848E-4 0.0 0.8170006757291484 0.0 70-71 7.609562480595615E-5 2.2828687441786848E-4 0.0 0.9327040732466045 0.0 72-73 7.609562480595615E-5 2.2828687441786848E-4 0.0 1.0489401401377028 0.0 74-75 7.609562480595615E-5 2.2828687441786848E-4 0.0 1.166203497963681 0.0 76-77 7.609562480595615E-5 2.2828687441786848E-4 0.0 1.2941963388872995 0.0 78-79 7.609562480595615E-5 2.2828687441786848E-4 0.0 1.4430393810077495 0.0 80-81 7.609562480595615E-5 2.2828687441786848E-4 0.0 1.6119716680769722 0.0 82-83 7.609562480595615E-5 2.2828687441786848E-4 0.0 1.785888218570985 0.0 84-85 7.609562480595615E-5 2.2828687441786848E-4 0.0 1.9510157243999098 0.0 86-87 7.609562480595615E-5 2.2828687441786848E-4 0.0 2.12770976519934 0.0 88-89 7.609562480595615E-5 2.2828687441786848E-4 0.0 2.3189000225243053 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1190 0.0 67.77837 1 GTATCAA 2255 0.0 44.880543 1 ATCAACG 2735 0.0 35.630203 3 TCAACGC 2805 0.0 35.27901 4 TATCAAC 2835 0.0 34.240887 2 CAACGCA 2905 0.0 33.877083 5 AACGCAG 3140 0.0 30.859955 6 TGGTATC 500 0.0 29.789572 2 ACGCAGA 3665 0.0 25.542393 7 CGCAGAG 3695 0.0 25.170937 8 GTGGTAT 615 0.0 25.0602 1 GTACATG 2180 0.0 24.744072 1 GAGTACT 2605 0.0 23.14451 12-13 TACATGG 2125 0.0 22.719835 2 GCAGAGT 3970 0.0 22.646997 9 ACATGGG 2140 0.0 22.178513 3 CGCGCCC 50 0.0027523406 21.75779 94-95 CAGAGTA 3735 0.0 20.830456 10-11 AGAGTAC 3450 0.0 20.097128 10-11 GTACTAG 285 1.736953E-8 19.828335 1 >>END_MODULE