FastQCFastQC Report
Sat 18 Jun 2016
SRR1930980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314136
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.2893916611370513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34890.26549763494798106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.14344025275922734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.062.2210161
GTATCAA35550.037.433171
GTGGTAT9350.029.481841
ATCAACG45300.029.1631984
TCAACGC46250.028.516355
CAACGCA46250.028.4137736
TATCAAC47550.027.9831123
AACGCAG48750.026.9566546
TGGTATC10150.026.6899052
CGCAGAG55150.023.6563669
ACGCAGA55600.023.4649038
GAGTACT44900.020.86803812-13
CAGAGTA61300.020.31560310-11
GCAGAGT62850.019.9278079
ACATGGG32850.019.8186363
TACATGG33900.019.7677672
ACTGCGC1200.004797779519.7674038
GTACTTT48950.019.28684614-15
GTACATG35600.019.0908031
CTACCGT1250.006015973619.0085284
Produced by FastQC (version 0.11.5)