##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930980_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1314136 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37508674901228 31.0 31.0 34.0 30.0 34.0 2 31.529760998861608 31.0 31.0 34.0 30.0 34.0 3 31.592283447070926 31.0 31.0 34.0 30.0 34.0 4 35.36320137337383 37.0 35.0 37.0 33.0 37.0 5 35.23722963224507 37.0 35.0 37.0 33.0 37.0 6 35.26668016095746 37.0 35.0 37.0 32.0 37.0 7 35.149655743393375 37.0 35.0 37.0 32.0 37.0 8 35.15282512616655 37.0 35.0 37.0 32.0 37.0 9 36.658202043015336 39.0 35.0 39.0 32.0 39.0 10-11 36.60778640871264 39.0 35.0 39.0 32.0 39.0 12-13 36.49412960302435 38.0 35.0 39.0 32.0 39.0 14-15 37.564947615771885 39.0 36.0 41.0 32.0 41.0 16-17 37.51426260295738 39.0 36.0 41.0 32.0 41.0 18-19 37.554016098790385 39.0 36.0 41.0 32.0 41.0 20-21 37.542799603693986 39.0 36.0 41.0 32.0 41.0 22-23 37.44080255011658 39.0 36.0 41.0 32.0 41.0 24-25 37.31969902658477 39.0 36.0 41.0 31.5 41.0 26-27 37.069770556472086 39.0 36.0 40.0 31.0 41.0 28-29 36.889853865962124 39.0 36.0 40.0 30.5 41.0 30-31 36.65422490518485 38.5 35.5 40.0 30.0 41.0 32-33 36.48674946885254 38.0 35.0 40.0 30.0 41.0 34-35 36.54864793293845 38.5 35.0 40.0 30.0 41.0 36-37 36.59315131767184 39.0 35.0 40.0 30.0 41.0 38-39 36.56955254250701 39.0 35.0 40.0 30.0 41.0 40-41 36.460168886629695 39.0 35.0 40.0 30.0 41.0 42-43 36.31877750856837 39.0 35.0 40.0 30.0 41.0 44-45 36.14402124285462 39.0 35.0 40.0 30.0 41.0 46-47 35.89731313958373 38.0 35.0 40.0 29.0 41.0 48-49 35.67075972349893 38.0 35.0 40.0 28.0 41.0 50-51 35.40837782390864 38.0 34.5 40.0 27.0 41.0 52-53 35.210438265141505 38.0 34.0 40.0 26.5 41.0 54-55 35.06405767743978 38.0 34.0 40.0 26.0 41.0 56-57 34.90180087905666 38.0 34.0 40.0 26.0 41.0 58-59 34.7017915192948 38.0 33.5 40.0 26.0 41.0 60-61 34.40820356492783 38.0 33.0 40.0 25.5 41.0 62-63 34.160848648846084 37.0 33.0 40.0 25.0 41.0 64-65 33.85595022128608 37.0 33.0 40.0 24.0 41.0 66-67 33.538439324392606 36.5 32.0 39.0 23.0 41.0 68-69 33.1947439230034 36.0 32.0 39.0 22.5 40.5 70-71 32.83234688038377 35.5 31.5 39.0 22.0 40.0 72-73 32.41987358994807 35.0 31.0 38.0 22.0 40.0 74-75 31.90348259236487 35.0 31.0 37.5 20.0 39.5 76-77 30.502590295068394 33.5 29.5 36.0 19.5 39.0 78-79 31.241378365709487 34.0 30.5 36.0 20.0 39.0 80-81 31.197514564702587 34.0 31.0 36.0 20.0 38.5 82-83 30.923283434895627 34.0 31.0 36.0 20.0 37.5 84-85 30.58063358739126 34.0 30.0 35.0 18.5 37.0 86-87 30.240804604698447 34.0 30.0 35.0 18.0 36.5 88-89 29.8858824353035 34.0 30.0 35.0 13.5 36.0 90-91 29.542508918407226 34.0 29.0 35.0 7.5 36.0 92-93 29.231240906572836 34.0 29.0 35.0 4.5 35.5 94-95 28.857463763263468 34.0 29.0 35.0 2.0 35.0 96-97 28.446955642338388 34.0 29.0 35.0 2.0 35.0 98-99 27.960991480333846 34.0 29.0 35.0 2.0 35.0 100-101 26.833964292889014 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2707949558357363 1101 2 0.33750117000458957 1101 3 0.31157908930201117 1101 4 0.13521091359751125 1101 5 0.28524614335545095 1101 6 0.2559770844490501 1101 7 0.438612375412319 1101 8 0.35962128815810246 1101 9 0.5104177588110019 1101 10-11 0.4327539307177304 1101 12-13 0.5324006554973764 1101 14-15 0.46785165416137886 1101 16-17 0.5482359091333109 1101 18-19 0.4555088933678846 1101 20-21 0.44136559925318863 1101 22-23 0.5233200373075704 1101 24-25 0.385449659100793 1101 26-27 0.3967214513561501 1101 28-29 0.4409954372180991 1101 30-31 0.37761825016460193 1101 32-33 0.30102531787014186 1101 34-35 0.16810929868578484 1101 36-37 0.3820713272020271 1101 38-39 0.21708453463201494 1101 40-41 0.27902535861827715 1101 42-43 0.3509489639888912 1101 44-45 0.39119177908614233 1101 46-47 0.23205825444005512 1101 48-49 0.294315687888691 1101 50-51 0.30890051893871373 1101 52-53 0.045973314310529645 1101 54-55 -0.012400126342498652 1101 56-57 0.07743306023380825 1101 58-59 0.20298537405702888 1101 60-61 0.12809821240905706 1101 62-63 -0.27166203583137616 1101 64-65 0.05980029376580376 1101 66-67 0.1448202313578406 1101 68-69 0.02419649636007648 1101 70-71 0.008434013463990198 1101 72-73 -0.044553977501330166 1101 74-75 -0.08193642805859369 1101 76-77 0.14812844591114072 1101 78-79 0.23280889694727236 1101 80-81 0.022762368316282533 1101 82-83 -0.004153811553347708 1101 84-85 0.005571471443783338 1101 86-87 0.014294012972424497 1101 88-89 -0.1017993553701011 1101 90-91 -0.006933744109808515 1101 92-93 -0.19828366129463504 1101 94-95 0.00885580477656589 1101 96-97 0.04529044674214333 1101 98-99 0.14897845887604788 1101 100-101 0.25567729440939146 1102 1 0.3162496047761074 1102 2 0.4254621566757173 1102 3 0.3263377205115354 1102 4 0.1348187540563046 1102 5 0.2666239722828223 1102 6 0.23512945066800484 1102 7 0.3067887140195964 1102 8 0.22896044525983683 1102 9 0.3198931074004179 1102 10-11 0.435180311159165 1102 12-13 0.37652093748797455 1102 14-15 0.36155165951159063 1102 16-17 0.4349965688720161 1102 18-19 0.4609968502565138 1102 20-21 0.409446430790787 1102 22-23 0.38048289678241076 1102 24-25 0.5039928290931357 1102 26-27 0.3799229075259163 1102 28-29 0.3263835385626024 1102 30-31 0.3492142882718099 1102 32-33 0.5357114593276151 1102 34-35 0.3422716766410687 1102 36-37 0.5725655279080257 1102 38-39 0.46790841117176996 1102 40-41 0.5447828867723743 1102 42-43 0.6133367090316924 1102 44-45 0.6418494573878206 1102 46-47 0.5759451758909009 1102 48-49 0.4817901462930294 1102 50-51 0.4309688560115035 1102 52-53 0.42035562034523366 1102 54-55 0.5369345684503699 1102 56-57 0.4352049338002004 1102 58-59 0.3448415465571415 1102 60-61 0.24072398520240768 1102 62-63 0.24912213526629756 1102 64-65 0.2515785466125209 1102 66-67 0.05555897114263075 1102 68-69 0.12698782991880364 1102 70-71 0.28931956548688476 1102 72-73 0.21811949190964697 1102 74-75 0.09340584355258486 1102 76-77 0.2814777729790272 1102 78-79 0.267447284869899 1102 80-81 0.08280973481413056 1102 82-83 0.014974068884097136 1102 84-85 0.17455865255091396 1102 86-87 0.17109806638807967 1102 88-89 0.32991792776681095 1102 90-91 0.18341873275619136 1102 92-93 0.37344510773420936 1102 94-95 0.5276592571465386 1102 96-97 0.43781198136658617 1102 98-99 0.4024404478591528 1102 100-101 0.6684587926751249 1103 1 0.3653430257564416 1103 2 0.449700768228837 1103 3 0.42093446694190817 1103 4 0.21564272106981974 1103 5 0.26981317474671584 1103 6 0.2764018123711054 1103 7 0.3954068272093707 1103 8 0.4561759821930167 1103 9 0.5607535865208959 1103 10-11 0.48246578851347266 1103 12-13 0.3725876999204232 1103 14-15 0.5227852439217315 1103 16-17 0.597852508649467 1103 18-19 0.46848205646133323 1103 20-21 0.6408640559563281 1103 22-23 0.38098331225340587 1103 24-25 0.4613361247593417 1103 26-27 0.4526076239122929 1103 28-29 0.5385168055102767 1103 30-31 0.6650913918390273 1103 32-33 0.7876079919109742 1103 34-35 0.7512725950506436 1103 36-37 0.6313086973069346 1103 38-39 0.5668182623759606 1103 40-41 0.8347880899718874 1103 42-43 0.7055121174099597 1103 44-45 0.7979919801994768 1103 46-47 0.8600137975700761 1103 48-49 0.6351791224625956 1103 50-51 0.4891832951144437 1103 52-53 0.5477916764994148 1103 54-55 0.6010569939038888 1103 56-57 0.5583864772441203 1103 58-59 0.8721398412757821 1103 60-61 0.7518573183114654 1103 62-63 0.5079785078961265 1103 64-65 0.6187172578562539 1103 66-67 0.4576726588093649 1103 68-69 0.48775766795800735 1103 70-71 0.6900859160367574 1103 72-73 0.6407622687136936 1103 74-75 0.8569179023038878 1103 76-77 0.5969708041679489 1103 78-79 0.8692458089105877 1103 80-81 0.8228005843598076 1103 82-83 0.7594100656051843 1103 84-85 0.8057821669757779 1103 86-87 0.6197178728604591 1103 88-89 0.5763511008550566 1103 90-91 0.6275743286066415 1103 92-93 0.6285260769198295 1103 94-95 0.7304648918527796 1103 96-97 1.0065659832886311 1103 98-99 1.0150120619387444 1103 100-101 1.245535951414201 1104 1 0.43342063201685477 1104 2 0.33325288243850437 1104 3 0.46943563004593614 1104 4 0.27498962303361907 1104 5 0.37331018587182285 1104 6 0.3596866601227191 1104 7 0.5177488732825921 1104 8 0.4244191176795127 1104 9 0.6641510601379608 1104 10-11 0.4332719873355231 1104 12-13 0.5554458012287071 1104 14-15 0.7842460805867262 1104 16-17 0.7516794783647711 1104 18-19 0.6592184690415479 1104 20-21 0.5527120440338251 1104 22-23 0.6726180370954324 1104 24-25 0.541269143165529 1104 26-27 0.6659640726779728 1104 28-29 0.7609707625386761 1104 30-31 0.8745383812418552 1104 32-33 0.7066268050499218 1104 34-35 0.558698588088518 1104 36-37 0.7029468317210714 1104 38-39 0.9040516145902586 1104 40-41 0.8391548169476479 1104 42-43 0.7084649718267357 1104 44-45 0.7839797387357805 1104 46-47 0.7843881135934154 1104 48-49 0.7653296385728297 1104 50-51 0.7448046896294329 1104 52-53 0.6794245315761032 1104 54-55 0.7713290997546025 1104 56-57 0.9363999615518352 1104 58-59 0.8900582425164316 1104 60-61 0.9351017461357714 1104 62-63 0.7345616217153363 1104 64-65 0.7581470344192951 1104 66-67 0.8395243864175441 1104 68-69 0.7423429924997791 1104 70-71 0.9160036133657385 1104 72-73 0.8582473243392172 1104 74-75 0.9832703200754587 1104 76-77 0.9805798362344227 1104 78-79 1.1189065014936723 1104 80-81 0.8301421629916881 1104 82-83 0.7349491354519166 1104 84-85 0.744450839057432 1104 86-87 0.8678475575850264 1104 88-89 1.040564892024264 1104 90-91 1.060255352138185 1104 92-93 0.8661663780620366 1104 94-95 0.9544713040480488 1104 96-97 1.1566575467974118 1104 98-99 1.2136155122057382 1104 100-101 1.587923806738356 1105 1 0.18577654773287477 1105 2 0.2721944287167126 1105 3 0.28240408652788673 1105 4 0.25092157843160834 1105 5 0.16291108841679147 1105 6 0.3118067564870799 1105 7 0.29413156521408723 1105 8 0.29450516494521395 1105 9 0.3466663950952338 1105 10-11 0.38760985751160604 1105 12-13 0.36661291943584473 1105 14-15 0.4633908066745107 1105 16-17 0.5370594824916068 1105 18-19 0.5022608602497698 1105 20-21 0.47304610358357024 1105 22-23 0.5014110439797506 1105 24-25 0.42035089476735976 1105 26-27 0.2989530561625813 1105 28-29 0.29813240866494084 1105 30-31 0.35695349297467516 1105 32-33 0.5548258687399041 1105 34-35 0.3779234927558619 1105 36-37 0.2922459926865315 1105 38-39 0.31662507365818016 1105 40-41 0.04939018073919499 1105 42-43 0.07672261518748513 1105 44-45 0.10691156282526038 1105 46-47 0.15750845917595058 1105 48-49 0.07042789216973944 1105 50-51 0.011575599168594408 1105 52-53 -0.08604508390436649 1105 54-55 0.13726472643113397 1105 56-57 0.08638991243173422 1105 58-59 0.29095891165383136 1105 60-61 0.15822383356579195 1105 62-63 -0.005584747068610341 1105 64-65 0.1138548006472817 1105 66-67 0.2698796965065995 1105 68-69 0.04064348530862816 1105 70-71 0.08197166520312038 1105 72-73 0.0521487143335051 1105 74-75 0.26780351834861094 1105 76-77 0.22579926882090717 1105 78-79 0.3132521965846067 1105 80-81 0.14742906135086642 1105 82-83 0.28147254186245263 1105 84-85 0.2011434190551249 1105 86-87 0.24264677616544716 1105 88-89 0.22633373010480184 1105 90-91 0.24571842899117513 1105 92-93 0.1903992282336766 1105 94-95 0.16597151708939606 1105 96-97 0.3008005176388835 1105 98-99 0.6148259622187346 1105 100-101 0.6956361714437058 1106 1 0.32608155862612165 1106 2 0.3334777233965909 1106 3 0.30183388951240175 1106 4 0.21426404803593613 1106 5 0.3109842722901064 1106 6 0.3128562563348041 1106 7 0.4000935676972759 1106 8 0.25851294248872847 1106 9 0.4146543071059128 1106 10-11 0.38159163182228184 1106 12-13 0.46163950832707457 1106 14-15 0.4622288604145268 1106 16-17 0.5083341094272029 1106 18-19 0.49840957062998115 1106 20-21 0.4129270977972652 1106 22-23 0.4206440657428203 1106 24-25 0.5967863434988274 1106 26-27 0.6811818377588494 1106 28-29 0.7075287119891343 1106 30-31 0.8270122930777504 1106 32-33 0.6139843947548513 1106 34-35 0.6225600331545778 1106 36-37 0.5155762805961999 1106 38-39 0.4959560175052573 1106 40-41 0.5106687901519038 1106 42-43 0.6004698918759388 1106 44-45 0.5808544494183678 1106 46-47 0.3821225102922199 1106 48-49 0.5227037607011411 1106 50-51 0.09171709423735308 1106 52-53 0.1181646286544975 1106 54-55 0.33274110097138276 1106 56-57 0.6070238290808376 1106 58-59 0.48398489146033796 1106 60-61 0.43609765932071554 1106 62-63 0.3544944339466909 1106 64-65 0.23857661290863064 1106 66-67 0.2018238481218404 1106 68-69 0.3799575621938587 1106 70-71 0.33159567251214384 1106 72-73 0.324037111269341 1106 74-75 0.6490601070055853 1106 76-77 0.4984255953371779 1106 78-79 0.6549299907608166 1106 80-81 0.6598965573098248 1106 82-83 0.822649012450686 1106 84-85 0.8381500018555101 1106 86-87 0.6393998860115353 1106 88-89 0.7753041426425789 1106 90-91 0.7454396555941258 1106 92-93 0.8429257585590655 1106 94-95 0.9440585225008782 1106 96-97 0.9047642536709297 1106 98-99 1.0909264143470132 1106 100-101 1.1897093084643409 1107 1 0.21169875476963185 1107 2 0.23992066888877517 1107 3 0.09847144387491369 1107 4 0.10871435572902755 1107 5 0.13731841628129615 1107 6 0.113389557037884 1107 7 0.1368173882920658 1107 8 0.06390356231059258 1107 9 0.2369560655706593 1107 10-11 0.15444377088834216 1107 12-13 0.35639088109203243 1107 14-15 0.33430093248659887 1107 16-17 0.38042581063606207 1107 18-19 0.2947848083464777 1107 20-21 0.3936793271063692 1107 22-23 0.3505129838969623 1107 24-25 0.26669181773366546 1107 26-27 0.3423806426758276 1107 28-29 0.30420272046280417 1107 30-31 0.2666748758642754 1107 32-33 0.2190726748855738 1107 34-35 0.19303220311741853 1107 36-37 0.21315348428545633 1107 38-39 0.25630159228207106 1107 40-41 0.2678596822324337 1107 42-43 0.2265248942467295 1107 44-45 0.30619082144404786 1107 46-47 0.3090914146804806 1107 48-49 0.4350288192061669 1107 50-51 0.45807963674590724 1107 52-53 0.5866198410825376 1107 54-55 0.5368813192881774 1107 56-57 0.5388454639585021 1107 58-59 0.36288739328304587 1107 60-61 0.44648784577201894 1107 62-63 0.2336480357658246 1107 64-65 0.32294366247092654 1107 66-67 0.4011939312780015 1107 68-69 0.46005438227370377 1107 70-71 0.4017930345600931 1107 72-73 0.24286884623010963 1107 74-75 0.6242218561911024 1107 76-77 0.4000168300353728 1107 78-79 0.4763458888320642 1107 80-81 0.3685201214512155 1107 82-83 0.4471630001225684 1107 84-85 0.49241895671869074 1107 86-87 0.5862791081878704 1107 88-89 0.630083455061957 1107 90-91 0.6251724444101043 1107 92-93 0.27748476578360837 1107 94-95 0.364031806480952 1107 96-97 0.4652288778876752 1107 98-99 0.6217464534013466 1107 100-101 0.7386355691902651 1108 1 -0.012111377249652833 1108 2 0.08303845415374411 1108 3 0.047406029590625565 1108 4 -0.04196397228075455 1108 5 0.03354623066528717 1108 6 0.09577393844661941 1108 7 0.08064970721489573 1108 8 0.019469498306420974 1108 9 0.03744491907220748 1108 10-11 -0.022221251159500355 1108 12-13 0.10381013425654828 1108 14-15 0.07770074069452448 1108 16-17 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2316 70-71 -1.5553549028510005 2316 72-73 -2.1479917512094353 2316 74-75 -3.3282319133679295 2316 76-77 -2.258672122074632 2316 78-79 -2.263151503296907 2316 80-81 -1.7929189491983841 2316 82-83 -1.5317369524615927 2316 84-85 -1.4853785776577872 2316 86-87 -1.3889579709965751 2316 88-89 -1.5417633917848867 2316 90-91 -1.1753017139587882 2316 92-93 -1.0180344376303303 2316 94-95 -1.6470516453919757 2316 96-97 -1.3829456123920778 2316 98-99 -1.2761316878978413 2316 100-101 -1.644543662673179 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 7.0 5 25.0 6 63.0 7 161.0 8 467.0 9 853.0 10 1522.0 11 2534.0 12 3683.0 13 4982.0 14 5804.0 15 7261.0 16 8576.0 17 10090.0 18 8616.0 19 6659.0 20 6355.0 21 6715.0 22 7372.0 23 8595.0 24 10431.0 25 13293.0 26 17233.0 27 22203.0 28 27945.0 29 35146.0 30 43064.0 31 52182.0 32 63309.0 33 77052.0 34 95267.0 35 120051.0 36 157797.0 37 220898.0 38 221643.0 39 46251.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T 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20.97865061150444 18-19 20.097919850000302 28.88936152726963 30.96232049042108 20.050398132308985 20-21 20.83003585625841 28.198945923405187 31.045378864896783 19.92563935543962 22-23 20.458462664681072 27.97293420518959 30.99436053178869 20.574242598340653 24-25 20.341710263483307 28.31366879102064 30.98982212498811 20.354798820507945 26-27 20.25205825123031 28.369699132715066 30.887884568994316 20.490358047060308 28-29 20.228386233209942 28.44060363730169 31.006971513929138 20.324038615559225 30-31 20.562374576976726 28.202895925811212 30.978925507570853 20.255803989641212 32-33 20.38406182618312 28.50807191236318 30.687119113889654 20.420747147564043 34-35 20.53467360700721 28.432171286731982 30.83231954080811 20.200835565452692 36-37 20.657693381821947 28.219580663301684 30.75366613323106 20.369059821645312 38-39 20.39824827071903 28.507453600480925 30.79105567942289 20.303242449377155 40-41 20.52934625410401 28.495146720807174 30.795940831504787 20.17956619358403 42-43 20.487326534425225 28.55126635720105 30.456081897402974 20.505325210970746 44-45 20.50205415886789 28.73094190708589 30.33720776703019 20.429796167016036 46-47 20.661987560859135 28.60618075757374 30.09720338128497 20.634628300282152 48-49 20.564573820395736 28.835388127853882 29.918911719939118 20.681126331811264 50-51 20.63061836998509 29.02867219959721 29.747754939286057 20.592954491131643 52-53 20.70543062351321 29.095975361924115 29.57727110835154 20.621322906211134 54-55 20.65776294839517 28.95579446962801 29.72144929477559 20.664993287201234 56-57 20.616886020068467 29.17214431854459 29.601275140281793 20.609694521105144 58-59 20.54194197523488 29.12506198129316 29.686175821758738 20.64682022171322 60-61 20.52520177650181 29.147780477528194 29.822197434297397 20.504820311672596 62-63 20.45279184642089 29.097814648874255 29.753492454041336 20.695901050663515 64-65 20.36123999310369 28.92208543185088 29.83271871753051 20.88395585751492 66-67 20.33460069663656 29.134834765300006 29.614721301507217 20.915843236556213 68-69 20.559355200469945 29.052826719662495 29.588608439916236 20.79920963995133 70-71 20.533521591098488 28.780935621260163 29.654106868319108 21.03143591932224 72-73 20.468798590235306 29.00962810748846 29.543587156019246 20.97798614625699 74-75 20.33625691951427 29.260379748284187 29.412675825286794 20.990687506914746 76-77 20.315373314782708 29.087596793116187 29.54025438349996 21.05677550860114 78-79 20.36877227347547 29.203166058248346 29.39744898075663 21.030612687519547 80-81 20.49480437900049 29.039889722178575 29.23016690440217 21.235138994418765 82-83 20.4198043429295 29.073284652425624 29.435537874314377 21.0713731303305 84-85 20.465271478751056 28.992813529193324 29.276079492533498 21.26583549952212 86-87 20.31072126477016 29.107794018275122 29.278780887214108 21.302703829740604 88-89 20.37085230969421 29.03400335581961 29.200424613909533 21.394719720576653 90-91 20.264332434088118 29.053661729062107 29.36003926746394 21.321966569385836 92-93 20.288145945236288 29.011638858604137 29.27672399522801 21.423491200931565 94-95 20.017639368513652 28.923465839133222 29.389904619566458 21.66899017278667 96-97 20.119424519008884 28.744638585594867 29.41511739086266 21.72081950453359 98-99 20.017562870205214 28.88346411634717 29.25112012759714 21.847852885850475 100-101 20.04339733482684 28.81288542433964 29.164371115318353 21.979346125515168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 222.0 1 165.5 2 179.5 3 233.0 4 173.0 5 163.0 6 271.5 7 547.0 8 927.0 9 1160.5 10 1078.5 11 924.5 12 937.0 13 1047.5 14 1177.5 15 1334.0 16 1503.5 17 1690.0 18 1927.0 19 2113.5 20 2486.5 21 3020.5 22 3616.0 23 4562.5 24 5879.5 25 7465.5 26 9947.0 27 12990.5 28 15917.0 29 19490.0 30 23168.0 31 26779.0 32 31394.0 33 35747.0 34 39228.0 35 42059.0 36 46450.0 37 51078.5 38 52386.0 39 52419.0 40 52578.0 41 52326.0 42 51739.0 43 52203.5 44 53119.5 45 53673.0 46 52888.0 47 50580.5 48 48941.5 49 47371.0 50 44928.5 51 41683.0 52 37792.0 53 34107.0 54 30669.5 55 26119.0 56 20905.0 57 16456.5 58 12867.0 59 9892.0 60 7040.0 61 4705.0 62 3209.5 63 2045.0 64 1106.0 65 637.0 66 374.0 67 188.0 68 95.0 69 60.5 70 36.5 71 14.5 72 10.5 73 9.5 74 6.5 75 5.0 76 3.5 77 1.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004185259364327588 2 0.006696414982924141 3 0.001902390620148904 4 0.17707451892346 5 0.0015980081209250793 6 6.087649984476492E-4 7 0.0 8 3.043824992238246E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.804781240297808E-4 24-25 8.370518728655176E-4 26-27 0.0026633468682084657 28-29 1.902390620148904E-4 30-31 0.006315936858894361 32-33 0.019708766824742645 34-35 0.013354782153445304 36-37 9.8924312247743E-4 38-39 4.5657374883573696E-4 40-41 0.0018643428077459257 42-43 0.011452391533296403 44-45 0.007191036544162857 46-47 0.010539244035624928 48-49 0.010349004973610037 50-51 0.19286436107069588 52-53 0.11669264063993376 54-55 0.017349802455758005 56-57 0.006430080296103296 58-59 0.01761613714257885 60-61 0.12700359780114082 62-63 0.24369623844107458 64-65 0.2503546056115957 66-67 0.2594480327759075 68-69 0.2542354824766995 70-71 0.24103289157286611 72-73 0.16314901958397 74-75 0.2686936511898312 76-77 0.21074683290009558 78-79 0.0055549806108348 80-81 3.804781240297808E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.609562480595615E-5 90-91 0.005402789361222887 92-93 0.01662689402010142 94-95 0.002282868744178685 96-97 3.804781240297808E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1314136.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.92723323476368 #Duplication Level Percentage of deduplicated Percentage of total 1 80.03046688349609 51.9615678923178 2 12.821860640502303 16.64975872619059 3 3.327671471285803 6.481695052345275 4 1.299674280218428 3.3753702048386187 5 0.6510369614412079 2.1135014319972574 6 0.42229886875307165 1.6451218287784521 7 0.2651499032611655 1.2050814727848902 8 0.19236330454325432 0.9991693711911798 9 0.14053455434799259 0.8212067808926117 >10 0.7750281538330872 9.161525760953886 >50 0.05097827768277423 2.275613854326859 >100 0.022230966908935848 2.430465191671529 >500 3.5286686296300994E-4 0.17908506356207315 >1k 3.5286686296300994E-4 0.7008373681488892 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3803 0.2893916611370513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3489 0.26549763494798106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1885 0.14344025275922734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.609562480595615E-5 0.0 0.0 0.0 0.0 7 7.609562480595615E-5 0.0 0.0 0.0 0.0 8 1.521912496119123E-4 0.0 0.0 0.0 0.0 9 1.521912496119123E-4 0.0 0.0 7.609562480595615E-5 0.0 10-11 1.521912496119123E-4 0.0 0.0 7.609562480595615E-5 0.0 12-13 1.521912496119123E-4 0.0 0.0 3.043824992238246E-4 0.0 14-15 1.521912496119123E-4 0.0 0.0 3.043824992238246E-4 0.0 16-17 1.521912496119123E-4 0.0 0.0 3.043824992238246E-4 0.0 18-19 2.2828687441786848E-4 0.0 0.0 3.8047812402978076E-4 0.0 20-21 2.2828687441786848E-4 0.0 0.0 3.8047812402978076E-4 0.0 22-23 2.2828687441786848E-4 0.0 0.0 4.1852593643275886E-4 0.0 24-25 3.043824992238246E-4 0.0 0.0 4.94621561238715E-4 0.0 26-27 3.043824992238246E-4 0.0 0.0 7.990040604625396E-4 0.0 28-29 3.043824992238246E-4 0.0 0.0 0.0012555778092982765 0.0 30-31 3.043824992238246E-4 0.0 0.0 0.00376673342789483 0.0 32-33 3.043824992238246E-4 0.0 0.0 0.011186056846475556 0.0 34-35 3.043824992238246E-4 0.0 0.0 0.021420918382876658 0.0 36-37 3.043824992238246E-4 0.0 0.0 0.042004784892887795 0.0 38-39 3.043824992238246E-4 0.0 0.0 0.07708486792843358 0.0 40-41 3.043824992238246E-4 0.0 0.0 0.11414343720893423 0.0 42-43 3.043824992238246E-4 0.0 0.0 0.15576774397779225 0.0 44-45 3.043824992238246E-4 0.0 0.0 0.20895858571715561 0.0 46-47 3.043824992238246E-4 0.0 0.0 0.26918827275106993 0.0 48-49 3.043824992238246E-4 0.0 0.0 0.33923429538495253 0.0 50-51 3.043824992238246E-4 0.0 0.0 0.41441677269323723 0.0 52-53 3.043824992238246E-4 0.0 0.0 0.4886100068790445 0.0 54-55 3.043824992238246E-4 0.0 0.0 0.5728859113516409 0.0 56-57 3.043824992238246E-4 0.0 0.0 0.675120383278443 0.0 58-59 3.043824992238246E-4 0.0 0.0 0.7977484826532413 0.0 60-61 3.043824992238246E-4 0.0 0.0 0.9296982960667693 0.0 62-63 3.043824992238246E-4 0.0 0.0 1.0576150413655816 0.0 64-65 3.043824992238246E-4 0.0 0.0 1.194320831329482 0.0 66-67 3.043824992238246E-4 0.0 0.0 1.353855308735169 0.0 68-69 3.043824992238246E-4 0.0 0.0 1.5435997491888207 0.0 70-71 3.043824992238246E-4 0.0 0.0 1.7522539524067524 0.0 72-73 3.043824992238246E-4 0.0 0.0 1.9671860446711755 0.0 74-75 3.043824992238246E-4 0.0 0.0 2.1828029975588525 0.0 76-77 3.043824992238246E-4 0.0 0.0 2.416264374463526 0.0 78-79 3.043824992238246E-4 0.0 0.0 2.689752050016132 0.0 80-81 3.043824992238246E-4 0.0 0.0 3.002390924531403 0.0 82-83 3.043824992238246E-4 0.0 0.0 3.3229056962140904 0.0 84-85 3.043824992238246E-4 0.0 0.0 3.6406810254037634 0.0 86-87 3.043824992238246E-4 0.0 0.0 3.9803338467251486 0.0 88-89 3.043824992238246E-4 0.0 0.0 4.357577906700676 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2070 0.0 62.221016 1 GTATCAA 3555 0.0 37.43317 1 GTGGTAT 935 0.0 29.48184 1 ATCAACG 4530 0.0 29.163198 4 TCAACGC 4625 0.0 28.51635 5 CAACGCA 4625 0.0 28.413773 6 TATCAAC 4755 0.0 27.983112 3 AACGCAG 4875 0.0 26.956654 6 TGGTATC 1015 0.0 26.689905 2 CGCAGAG 5515 0.0 23.656366 9 ACGCAGA 5560 0.0 23.464903 8 GAGTACT 4490 0.0 20.868038 12-13 CAGAGTA 6130 0.0 20.315603 10-11 GCAGAGT 6285 0.0 19.927807 9 ACATGGG 3285 0.0 19.818636 3 TACATGG 3390 0.0 19.767767 2 ACTGCGC 120 0.0047977795 19.767403 8 GTACTTT 4895 0.0 19.286846 14-15 GTACATG 3560 0.0 19.090803 1 CTACCGT 125 0.0060159736 19.008528 4 >>END_MODULE