FastQCFastQC Report
Sat 18 Jun 2016
SRR1930979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758838
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1222634.431684644042166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.054.6189771
GTATCAA44400.045.6283261
ATCAACG53100.036.6493033
TCAACGC54050.035.7233664
TATCAAC54900.035.6801952
CAACGCA55000.035.099635
AACGCAG60550.032.003636
ACGCAGA66700.028.769957
CGCAGAG68350.027.4623368
GTGGTAT11750.024.0074081
GCAGAGT74950.023.7487559
GTACATG41550.023.4532491
TGGTATC12750.022.5223662
TACATGG42850.022.4979612
ACATGGG44550.020.9361573
GAGTACT48100.020.87289412-13
CAGAGTA69800.020.37724910-11
AGAGTAC63500.019.98229610-11
GTATACG4500.019.3756731
GTACTTT54750.018.82754514-15
Produced by FastQC (version 0.11.5)