FastQCFastQC Report
Sat 18 Jun 2016
SRR1930979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758838
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78790.28559125254908047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70270.25470868532331364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40910.14828706868616426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.059.8209231
GTATCAA75950.040.7453731
ATCAACG94200.032.143263
CAACGCA96200.031.415675
TCAACGC97050.031.2477444
TATCAAC98950.030.985122
AACGCAG101850.029.8581736
GTGGTAT18900.028.4211541
ACGCAGA115950.025.7772187
CGCAGAG116700.025.5297878
GCGCGTA601.7263292E-423.72074712-13
TGGTATC21150.022.924462
GCAGAGT133000.022.1155859
TACATGG69700.021.0052192
GTACATG69700.020.7331771
GAGTACT96750.020.59475712-13
CAGAGTA128500.020.34261710-11
AGAGTAC121650.020.18164810-11
ACATGGG71300.019.5334743
GTACTTT106150.018.79335814-15
Produced by FastQC (version 0.11.5)