##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930979_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2758838 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33644672140952 31.0 31.0 34.0 30.0 34.0 2 31.487326548351152 31.0 31.0 34.0 30.0 34.0 3 31.54943240596222 31.0 31.0 34.0 30.0 34.0 4 35.322333170704475 37.0 35.0 37.0 33.0 37.0 5 35.1980076394482 37.0 35.0 37.0 33.0 37.0 6 35.227256547865444 37.0 35.0 37.0 32.0 37.0 7 35.10974439238549 37.0 35.0 37.0 32.0 37.0 8 35.11259305548205 37.0 35.0 37.0 32.0 37.0 9 36.619502848663096 39.0 35.0 39.0 32.0 39.0 10-11 36.560167360316186 38.0 35.0 39.0 32.0 39.0 12-13 36.43947814260932 38.0 35.0 39.0 32.0 39.0 14-15 37.494534293061065 39.0 36.0 41.0 32.0 41.0 16-17 37.45030643336071 39.0 36.0 41.0 32.0 41.0 18-19 37.48341566268117 39.0 36.0 41.0 32.0 41.0 20-21 37.4691382748824 39.0 36.0 41.0 32.0 41.0 22-23 37.36669441989707 39.0 36.0 41.0 32.0 41.0 24-25 37.239247465780885 39.0 36.0 40.5 31.0 41.0 26-27 36.981942216251916 39.0 36.0 40.0 31.0 41.0 28-29 36.79492289869866 39.0 36.0 40.0 30.0 41.0 30-31 36.56258558857026 38.0 35.0 40.0 30.0 41.0 32-33 36.38840754694549 38.0 35.0 40.0 30.0 41.0 34-35 36.46007666996032 38.0 35.0 40.0 30.0 41.0 36-37 36.51848749364768 38.0 35.0 40.0 30.0 41.0 38-39 36.5000367908518 39.0 35.0 40.0 30.0 41.0 40-41 36.37802491483733 39.0 35.0 40.0 30.0 41.0 42-43 36.22593570191508 38.0 35.0 40.0 30.0 41.0 44-45 36.04488157695377 38.0 35.0 40.0 29.5 41.0 46-47 35.790023372158856 38.0 35.0 40.0 28.5 41.0 48-49 35.55736654345054 38.0 34.5 40.0 27.5 41.0 50-51 35.28622249657283 38.0 34.0 40.0 26.5 41.0 52-53 35.07298199459338 38.0 34.0 40.0 26.0 41.0 54-55 34.91521086051446 38.0 34.0 40.0 26.0 41.0 56-57 34.734474260540125 38.0 33.5 40.0 26.0 41.0 58-59 34.52366992915133 38.0 33.0 40.0 26.0 41.0 60-61 34.21430598679589 37.0 33.0 40.0 25.0 41.0 62-63 33.95930315589389 37.0 33.0 40.0 24.0 41.0 64-65 33.64198948977794 36.5 32.0 39.5 23.0 41.0 66-67 33.322089771128276 36.0 32.0 39.0 23.0 41.0 68-69 32.96724073686095 36.0 31.5 39.0 22.0 40.0 70-71 32.58921636573079 35.0 31.0 38.5 22.0 40.0 72-73 32.16124125447018 35.0 31.0 38.0 21.5 40.0 74-75 31.648371524533154 35.0 30.5 37.0 20.0 39.0 76-77 30.27253865576739 33.5 28.5 36.0 18.5 38.5 78-79 31.003343799092228 34.0 30.0 36.0 20.0 39.0 80-81 30.962146381918764 34.0 30.0 36.0 20.0 38.5 82-83 30.686521463021748 34.0 30.0 35.5 18.5 37.0 84-85 30.34797639440953 34.0 30.0 35.0 18.0 37.0 86-87 30.00293873725097 34.0 30.0 35.0 16.0 36.5 88-89 29.65366088911346 34.0 29.0 35.0 10.0 36.0 90-91 29.314371304150516 34.0 29.0 35.0 7.0 36.0 92-93 28.988428099076494 34.0 29.0 35.0 2.0 35.0 94-95 28.602198461816172 34.0 29.0 35.0 2.0 35.0 96-97 28.16456475516141 34.0 29.0 35.0 2.0 35.0 98-99 27.66662341174074 33.0 28.0 35.0 2.0 35.0 100-101 26.52034896576022 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.301550066411842 1101 2 0.3480830437317195 1101 3 0.31457533014608074 1101 4 0.24090710071193655 1101 5 0.35619666050678944 1101 6 0.3082968310053644 1101 7 0.37521521627495247 1101 8 0.3047240684895982 1101 9 0.47618861774633814 1101 10-11 0.5492441517767475 1101 12-13 0.42682686908612055 1101 14-15 0.5645853650242074 1101 16-17 0.6528077295504957 1101 18-19 0.5217263492068227 1101 20-21 0.48587906583271234 1101 22-23 0.5242163336510473 1101 24-25 0.4249771616846161 1101 26-27 0.5205490463731053 1101 28-29 0.4536052293136734 1101 30-31 0.46047589136252753 1101 32-33 0.45459573731164227 1101 34-35 0.5588283591269487 1101 36-37 0.4216680125143597 1101 38-39 0.482199363423895 1101 40-41 0.57901780340827 1101 42-43 0.38646833657624313 1101 44-45 0.461641096741495 1101 46-47 0.4486381243184425 1101 48-49 0.5752936226730796 1101 50-51 0.5395844722783139 1101 52-53 0.33084120295889363 1101 54-55 0.5283321074296836 1101 56-57 0.39625138468827714 1101 58-59 0.3501413336852792 1101 60-61 0.4688675738912167 1101 62-63 0.35899132208790263 1101 64-65 0.2795696187409291 1101 66-67 0.2849550179185272 1101 68-69 0.2847847093028193 1101 70-71 0.3092758432300755 1101 72-73 0.2485433394183616 1101 74-75 0.22259154063397446 1101 76-77 0.10559348714073735 1101 78-79 0.11963411375935706 1101 80-81 0.2609455767226656 1101 82-83 0.26415782535375243 1101 84-85 0.19693580507575703 1101 86-87 0.19736276783152462 1101 88-89 0.3644400391766389 1101 90-91 0.3800059127491515 1101 92-93 0.2497050406878465 1101 94-95 0.409892276410428 1101 96-97 0.5450411400252797 1101 98-99 0.628416244429566 1101 100-101 0.8004398881082935 1102 1 0.31869698199625773 1102 2 0.42009463754834897 1102 3 0.46372532893460416 1102 4 0.1435751726897081 1102 5 0.2712949717105104 1102 6 0.24505429773018506 1102 7 0.33254756831345844 1102 8 0.2854704481981969 1102 9 0.4197406930031775 1102 10-11 0.4968915176260751 1102 12-13 0.5258602886360961 1102 14-15 0.5907289975111283 1102 16-17 0.6233688848131393 1102 18-19 0.6066535294581641 1102 20-21 0.5521119234601528 1102 22-23 0.6175959503807036 1102 24-25 0.5583406304608687 1102 26-27 0.5493299087010683 1102 28-29 0.5808147832081616 1102 30-31 0.5528814064009708 1102 32-33 0.6877699888219482 1102 34-35 0.6906704171000655 1102 36-37 0.6255958671534998 1102 38-39 0.5951840544169826 1102 40-41 0.8343099304354666 1102 42-43 0.8780122404335842 1102 44-45 0.9118850575381572 1102 46-47 0.8965467144844936 1102 48-49 0.8603667099558265 1102 50-51 0.6933223020014552 1102 52-53 0.6481014167034829 1102 54-55 0.7732924972419823 1102 56-57 0.6568071185998932 1102 58-59 0.6879909452056054 1102 60-61 0.6020691340692252 1102 62-63 0.5016996806602947 1102 64-65 0.5095850751439812 1102 66-67 0.5008854358370485 1102 68-69 0.5919720124381129 1102 70-71 0.6236841688907333 1102 72-73 0.5823223946293155 1102 74-75 0.7670728677645506 1102 76-77 0.6049505219746827 1102 78-79 0.7001963240149003 1102 80-81 0.8604110890554857 1102 82-83 0.7969736569102572 1102 84-85 0.7248444053374321 1102 86-87 0.7346261011244728 1102 88-89 0.7970938864177022 1102 90-91 0.8545481518378004 1102 92-93 0.779352233901971 1102 94-95 0.9509191327940947 1102 96-97 1.1496470926081415 1102 98-99 1.0877224175008955 1102 100-101 1.2546048637576455 1103 1 0.36592102516100056 1103 2 0.44164276399292746 1103 3 0.4252287643067838 1103 4 0.2778228742254001 1103 5 0.32257982122622764 1103 6 0.4057900019933953 1103 7 0.4694854145068277 1103 8 0.35132743726828153 1103 9 0.470780185671444 1103 10-11 0.43769196549012435 1103 12-13 0.4754395192040519 1103 14-15 0.5140807602043864 1103 16-17 0.5367285651568281 1103 18-19 0.5960739579495637 1103 20-21 0.5843705160300559 1103 22-23 0.4933137383256607 1103 24-25 0.5667276701000503 1103 26-27 0.6232989771804682 1103 28-29 0.706526048373938 1103 30-31 0.574754919175966 1103 32-33 0.6287768450154871 1103 34-35 0.6751255053821197 1103 36-37 0.5241211169571258 1103 38-39 0.6542852003940212 1103 40-41 0.7004147725704115 1103 42-43 0.721717670722505 1103 44-45 0.6702943624331752 1103 46-47 0.7177784108762211 1103 48-49 0.6080634162784833 1103 50-51 0.4861940207612889 1103 52-53 0.507668928847778 1103 54-55 0.5961197657705029 1103 56-57 0.5375388540028538 1103 58-59 0.691290342525896 1103 60-61 0.6032487535955084 1103 62-63 0.6106887351859598 1103 64-65 0.5909039986546105 1103 66-67 0.5245986232156739 1103 68-69 0.4397041926444416 1103 70-71 0.5055932659348841 1103 72-73 0.41024075502340196 1103 74-75 0.42636885668185087 1103 76-77 0.503803541431882 1103 78-79 0.5690808108640972 1103 80-81 0.5056100350747172 1103 82-83 0.38446425846353094 1103 84-85 0.4412719117968642 1103 86-87 0.45819874769947333 1103 88-89 0.4001275181180084 1103 90-91 0.5419982118097373 1103 92-93 0.3236086006989183 1103 94-95 0.43139902620184856 1103 96-97 0.6669720331316853 1103 98-99 0.7072569243825555 1103 100-101 0.9173046771810753 1104 1 0.30369917679701786 1104 2 0.4383202193889417 1104 3 0.39531706541874456 1104 4 0.2973770796063775 1104 5 0.2645627934562853 1104 6 0.31392797062795097 1104 7 0.3555061063553353 1104 8 0.3793749198893934 1104 9 0.5646870823557322 1104 10-11 0.5404860965531242 1104 12-13 0.4128554086318843 1104 14-15 0.4688693279032563 1104 16-17 0.47595944127339607 1104 18-19 0.5267938414086331 1104 20-21 0.510741385038763 1104 22-23 0.5668866499822514 1104 24-25 0.4561853336871664 1104 26-27 0.6160089021647224 1104 28-29 0.6101691817757313 1104 30-31 0.5647281134597435 1104 32-33 0.6074269350327057 1104 34-35 0.5235942726795102 1104 36-37 0.5142956885362722 1104 38-39 0.4833775793058308 1104 40-41 0.33118558484158456 1104 42-43 0.3710853327586179 1104 44-45 0.4948477983383768 1104 46-47 0.2829042017882628 1104 48-49 0.183673540675656 1104 50-51 0.23711561413171722 1104 52-53 0.2447986480029698 1104 54-55 0.3035976121590309 1104 56-57 0.4648697901710719 1104 58-59 0.34722307310922673 1104 60-61 0.355313714464323 1104 62-63 0.40513518081644406 1104 64-65 0.30717718822632634 1104 66-67 0.3758587136221436 1104 68-69 0.37610716994327476 1104 70-71 0.4360788117812433 1104 72-73 0.3113238901027344 1104 74-75 0.4143359305902088 1104 76-77 0.41029668779485107 1104 78-79 0.5047770394204569 1104 80-81 0.26764708873809084 1104 82-83 0.4559735047665221 1104 84-85 0.454031652052695 1104 86-87 0.3713864538746954 1104 88-89 0.49389793378075453 1104 90-91 0.39009634124242965 1104 92-93 0.40005092791665575 1104 94-95 0.6486594586243406 1104 96-97 0.568801806770832 1104 98-99 0.699413935524106 1104 100-101 0.9312375516569737 1105 1 0.29739713257672307 1105 2 0.27615544682590354 1105 3 0.291196874881944 1105 4 0.19900055222812085 1105 5 0.22735766596256468 1105 6 0.21012632429768985 1105 7 0.20917393277521512 1105 8 0.4420006644603589 1105 9 0.46166252601038593 1105 10-11 0.39513654594539815 1105 12-13 0.3428409243896695 1105 14-15 0.32971240901049015 1105 16-17 0.3935614929581206 1105 18-19 0.3897753710685876 1105 20-21 0.3860987750379863 1105 22-23 0.37795992687459545 1105 24-25 0.5319815578006555 1105 26-27 0.4041484472803347 1105 28-29 0.43295569133571377 1105 30-31 0.3075279289734212 1105 32-33 0.5060165178709752 1105 34-35 0.5174404142025253 1105 36-37 0.48531594360613184 1105 38-39 0.5977442364571885 1105 40-41 0.4334031006170065 1105 42-43 0.6245560309288152 1105 44-45 0.5770247740234922 1105 46-47 0.4041215328684089 1105 48-49 0.4378474391122964 1105 50-51 0.37564676314644174 1105 52-53 0.3432043133534748 1105 54-55 0.43256245144918637 1105 56-57 0.5007261545949291 1105 58-59 0.4602057926664571 1105 60-61 0.33980453536458555 1105 62-63 0.3670387331996139 1105 64-65 0.2818755028326123 1105 66-67 0.47383218506559643 1105 68-69 0.38030768144818694 1105 70-71 0.33288540611066963 1105 72-73 0.3234697810275833 1105 74-75 0.43496712407806726 1105 76-77 0.5371920925741058 1105 78-79 0.6209504249823503 1105 80-81 0.5939115809613291 1105 82-83 0.6323202275978872 1105 84-85 0.6253019828986979 1105 86-87 0.6506307913277034 1105 88-89 0.5058050906819993 1105 90-91 0.5438562018630897 1105 92-93 0.5928875954195405 1105 94-95 0.7836262558469222 1105 96-97 0.8639558253325319 1105 98-99 0.8807204984518542 1105 100-101 0.9566741307708071 1106 1 0.22064876710664905 1106 2 0.24708065533131318 1106 3 0.2948185463587656 1106 4 0.14279726119399783 1106 5 0.18047509649842652 1106 6 0.14968893410433282 1106 7 0.27552183654426443 1106 8 0.22869023986260828 1106 9 0.3267244466346497 1106 10-11 0.3084027690408675 1106 12-13 0.331141037411669 1106 14-15 0.337393339937357 1106 16-17 0.46200086141018204 1106 18-19 0.3521461975251512 1106 20-21 0.33826732212747856 1106 22-23 0.35434753955306064 1106 24-25 0.4854849465633393 1106 26-27 0.32973100962671253 1106 28-29 0.3093807557032946 1106 30-31 0.3233257689642173 1106 32-33 0.4141864595385627 1106 34-35 0.4512022667652147 1106 36-37 0.3233807963170463 1106 38-39 0.2230874621572525 1106 40-41 0.2300529050161586 1106 42-43 0.19356321160314138 1106 44-45 0.3253378669247198 1106 46-47 0.2534262618657195 1106 48-49 0.32206583546937395 1106 50-51 0.1814218665008056 1106 52-53 0.17788143828262548 1106 54-55 0.315884743989308 1106 56-57 0.32798273960111857 1106 58-59 0.33290397126329907 1106 60-61 0.35753584611506284 1106 62-63 0.1359669284111078 1106 64-65 0.04408556414782794 1106 66-67 -0.0033567235770064485 1106 68-69 0.0017385452506388788 1106 70-71 0.09655196671979382 1106 72-73 0.1840606116076131 1106 74-75 0.35523711200545094 1106 76-77 0.23927827929417944 1106 78-79 0.18684912695993106 1106 80-81 0.18370019452095576 1106 82-83 0.2340650370548012 1106 84-85 0.2962075399037438 1106 86-87 0.2681064600910297 1106 88-89 0.2937099774202494 1106 90-91 0.256058944173045 1106 92-93 0.24560706295012125 1106 94-95 0.27632955832237727 1106 96-97 0.3933802962454962 1106 98-99 0.3787125727683325 1106 100-101 0.5164774179873142 1107 1 0.1824702369629847 1107 2 0.17006908164399448 1107 3 0.19259095852696362 1107 4 0.05279177970176363 1107 5 0.06754807738725077 1107 6 0.14654250926879087 1107 7 0.12960915888878333 1107 8 0.13530226255624456 1107 9 0.1430634100634265 1107 10-11 0.12125011275941233 1107 12-13 0.2151632637300338 1107 14-15 0.28380965484523557 1107 16-17 0.24786275103360111 1107 18-19 0.22367540507062955 1107 20-21 0.23087946187919783 1107 22-23 0.22716087893171277 1107 24-25 0.20165173801147063 1107 26-27 0.31829329597125877 1107 28-29 0.27331611296101244 1107 30-31 0.352920652536838 1107 32-33 0.3135649707781525 1107 34-35 0.1940810126384278 1107 36-37 0.2639626624092628 1107 38-39 0.1944800406054341 1107 40-41 0.1168441070773909 1107 42-43 0.21279132504646014 1107 44-45 0.14468883386708598 1107 46-47 0.2585928011445091 1107 48-49 0.2578066366641423 1107 50-51 0.23844853125252996 1107 52-53 0.3517048233840825 1107 54-55 0.20797897644068541 1107 56-57 0.30997470950822503 1107 58-59 0.2572136131356828 1107 60-61 0.2082166400908676 1107 62-63 0.043509321434903825 1107 64-65 -0.011732113624375984 1107 66-67 0.017694816823500048 1107 68-69 -0.042847707875175445 1107 70-71 -0.0348466578131692 1107 72-73 -0.028961364841173065 1107 74-75 0.1343890893959916 1107 76-77 0.03957223538267485 1107 78-79 0.267329207709313 1107 80-81 0.08711117293684723 1107 82-83 0.16751233912457764 1107 84-85 0.170354686060886 1107 86-87 0.20095381070887086 1107 88-89 0.11172144566329933 1107 90-91 0.06884770135305729 1107 92-93 -0.05825147292456023 1107 94-95 0.2256527774382171 1107 96-97 0.19003539010642 1107 98-99 0.13301563616011336 1107 100-101 0.3500777349870603 1108 1 0.03645104457205406 1108 2 0.07072470332424885 1108 3 0.0435491059988351 1108 4 0.009170914572102618 1108 5 0.032227635580099445 1108 6 0.08901313560484425 1108 7 0.06988622067378003 1108 8 0.1244489729887519 1108 9 0.012340632910721183 1108 10-11 0.12917968870374352 1108 12-13 0.02840148756505556 1108 14-15 0.027608208055546868 1108 16-17 0.09855938875715964 1108 18-19 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-1.9096226149605222 2316 74-75 -3.0251414581583482 2316 76-77 -1.9976564939892754 2316 78-79 -1.7841900355295692 2316 80-81 -1.4901681591964184 2316 82-83 -1.1939059057502313 2316 84-85 -1.2382384690153607 2316 86-87 -1.0776579403525837 2316 88-89 -1.3276448796694034 2316 90-91 -1.3176445949185727 2316 92-93 -1.304482160645808 2316 94-95 -1.7790494882066525 2316 96-97 -1.6124008412713096 2316 98-99 -1.7046958816844118 2316 100-101 -2.0888608780131825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 33.0 5 77.0 6 154.0 7 357.0 8 960.0 9 1782.0 10 3217.0 11 5340.0 12 7344.0 13 9904.0 14 12314.0 15 15500.0 16 18683.0 17 21700.0 18 19212.0 19 14746.0 20 14104.0 21 14594.0 22 16383.0 23 19175.0 24 23267.0 25 29787.0 26 37776.0 27 48576.0 28 61408.0 29 76783.0 30 94605.0 31 113775.0 32 137163.0 33 165919.0 34 206147.0 35 256701.0 36 337191.0 37 460639.0 38 434092.0 39 79387.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.27146696922299 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20.481575938855418 28.687548888336323 30.426940617752834 20.403934555055425 20-21 21.075630392215853 27.81799801220659 30.716337820488192 20.390033775089368 22-23 20.7928874145108 27.637079948441873 30.582388608496068 20.987644028551255 24-25 20.688072787601516 27.848757159533516 30.7312379579469 20.731932094918065 26-27 20.708137276970596 28.105030750989663 30.45971886777201 20.72711310426773 28-29 20.631364097032225 28.14705187260134 30.5522691453932 20.66931488497323 30-31 20.918753328140017 27.894552924540168 30.493048650564962 20.693645096754853 32-33 20.822830128141216 28.093126707356202 30.271436780463223 20.81260638403936 34-35 20.900226193041867 28.049169752449217 30.498594427483646 20.552009627025274 36-37 20.90350764794081 27.995611883292625 30.44062948052506 20.660250988241508 38-39 20.754667055720127 28.09552591540081 30.50968117854201 20.640125850337053 40-41 20.888374009938403 28.127808066788628 30.352440360693972 20.631377562578997 42-43 20.855769767564947 28.17072401500217 30.147915523245317 20.825590694187564 44-45 20.822617029290083 28.39274107923196 30.000902616391294 20.783739275086663 46-47 20.938054297149854 28.383004762265546 29.728216994295213 20.950723946289386 48-49 21.06331317424291 28.443421687498212 29.55704739833181 20.93621773992707 50-51 21.097641465325573 28.633930978884038 29.27008803537757 20.998339520412824 52-53 21.13789290046204 28.725345487501848 29.175139233753946 20.961622378282165 54-55 21.051751002950304 28.656463509865638 29.31457147393997 20.97721401324409 56-57 21.058551700538235 28.769806746473648 29.20246308347253 20.96917846951559 58-59 20.981463022388553 28.678197829391937 29.398193087429213 20.942146060790297 60-61 21.021407369314595 28.8262401862779 29.321403776438938 20.83094866796857 62-63 21.04598639969887 28.671939605245374 29.337875487048613 20.944198508007144 64-65 20.770863900389006 28.62420816337505 29.395042995538756 21.209884940697187 66-67 20.854016253686584 28.69201938465072 29.230162929326553 21.223801432336145 68-69 20.944989757402606 28.701165975499528 29.246462783715405 21.107381483382465 70-71 20.97916440345493 28.531509538915188 29.26916820746727 21.220157850162604 72-73 20.950319151659073 28.66316452715943 29.209342903193658 21.177173417987834 74-75 20.91042614853099 28.859156257853286 29.084731275174807 21.145686318440912 76-77 20.892380367443046 28.848779421213454 29.008718082639273 21.25012212870422 78-79 20.846605035942797 28.800455578718733 29.056356116981654 21.296583268356816 80-81 20.84219198926794 28.91017644575893 28.96315167644041 21.28447988853272 82-83 20.969335640584912 28.717742759814097 29.135744831700883 21.177176767900107 84-85 21.01004118400573 28.80218048323243 28.930694734522287 21.25708359823955 86-87 20.79801351148563 28.86414497697944 28.944160548752773 21.393680962782156 88-89 20.889244594459402 28.755841231591873 28.93424980680258 21.420664367146145 90-91 20.863884646217382 28.7763688302207 29.071981385290098 21.287765138271823 92-93 20.882946434527383 28.746432642194016 28.994026850893146 21.376594072385462 94-95 20.624075561251335 28.76944912195423 29.015591558334812 21.59088375845962 96-97 20.711332218733432 28.666965705664072 28.99904398138709 21.622658094215407 98-99 20.606284239958995 28.687657629770214 28.977018585360938 21.72903954490985 100-101 20.62464341871469 28.73593882641895 28.90039212161062 21.73902563325574 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 301.0 1 221.5 2 210.0 3 287.0 4 247.0 5 247.5 6 433.5 7 961.0 8 1646.5 9 2082.0 10 2000.0 11 1732.5 12 1841.0 13 2081.5 14 2375.5 15 2764.0 16 3085.0 17 3465.5 18 3980.5 19 4619.0 20 5299.0 21 6234.5 22 7644.0 23 9614.5 24 12167.0 25 15552.5 26 19981.5 27 24720.0 28 29661.0 29 36327.5 30 43930.0 31 51226.0 32 59394.5 33 67431.0 34 73451.5 35 79157.0 36 87840.0 37 96808.5 38 100966.5 39 102373.0 40 104432.5 41 105248.5 42 106025.0 43 109375.5 44 112272.5 45 115359.0 46 116305.0 47 114598.5 48 112807.5 49 109415.5 50 103859.0 51 95777.5 52 87297.0 53 79327.5 54 71332.0 55 61417.0 56 49881.0 57 38780.5 58 29761.5 59 23000.0 60 16871.0 61 11714.5 62 8048.0 63 5127.5 64 2864.5 65 1594.0 66 903.0 67 517.0 68 289.5 69 152.5 70 90.5 71 54.0 72 39.5 73 30.5 74 18.0 75 13.0 76 9.0 77 7.5 78 6.0 79 1.0 80 2.5 81 2.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004458398789635346 2 0.006162014587300886 3 0.0020660872439773556 4 0.16927416542761844 5 0.0018848515208214473 6 5.437071694677252E-4 7 7.249428926236336E-5 8 7.249428926236335E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.624714463118168E-4 24-25 5.980778864144977E-4 26-27 0.002356064401026809 28-29 7.249428926236336E-5 30-31 0.005980778864144977 32-33 0.020044670981043466 34-35 0.012813365627122722 36-37 8.518078988327695E-4 38-39 1.9935929547149924E-4 40-41 0.0016311215084031756 42-43 0.01101913196787923 44-45 0.0069232046245557 46-47 0.010040459062837324 48-49 0.01018544764136205 50-51 0.18542226836080988 52-53 0.11347168626791426 54-55 0.01725364084444248 56-57 0.006596980322875066 58-59 0.017634235863069884 60-61 0.12269658457654997 62-63 0.23727380875571527 64-65 0.24256589187186778 66-67 0.2526425980793363 68-69 0.2483110642959101 70-71 0.23491774435468846 72-73 0.16044798570992572 74-75 0.2611063063507172 76-77 0.2028027742114615 78-79 0.00540082455004607 80-81 5.4370716946772515E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.249428926236336E-5 90-91 0.005781419568673478 92-93 0.01790608944780375 94-95 0.002519176551867127 96-97 1.812357231559084E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2758838.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.62142424689208 #Duplication Level Percentage of deduplicated Percentage of total 1 73.27515790267614 41.48943802365433 2 15.510572835607128 17.564614496744625 3 5.041059638843786 8.562959293945758 4 2.194245102877118 4.969651314866827 5 1.1743396957860055 3.3246393062532804 6 0.6995308804317601 2.376506085283718 7 0.43801011051975736 1.7360529404517089 8 0.3078801326268985 1.3946089285325618 9 0.21798013844611816 1.1108111306718536 >10 1.0388428262009162 10.446196280482937 >50 0.07026071726324828 2.7176901138071434 >100 0.030773693751422828 3.106595702423973 >500 9.616606927937836E-4 0.35886574165715046 >1k 2.564428513731946E-4 0.30027689810191227 >5k 1.282214256865973E-4 0.5410937431222321 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7879 0.28559125254908047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7027 0.25470868532331364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4091 0.14828706868616426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.624714463118168E-5 2 0.0 0.0 0.0 0.0 3.624714463118168E-5 3 0.0 0.0 0.0 0.0 3.624714463118168E-5 4 0.0 0.0 0.0 3.624714463118168E-5 3.624714463118168E-5 5 0.0 0.0 0.0 3.624714463118168E-5 3.624714463118168E-5 6 0.0 0.0 0.0 7.249428926236336E-5 3.624714463118168E-5 7 0.0 0.0 0.0 7.249428926236336E-5 3.624714463118168E-5 8 0.0 0.0 0.0 7.249428926236336E-5 3.624714463118168E-5 9 0.0 0.0 0.0 7.249428926236336E-5 3.624714463118168E-5 10-11 0.0 0.0 0.0 1.4498857852472672E-4 3.624714463118168E-5 12-13 0.0 0.0 0.0 1.4498857852472672E-4 3.624714463118168E-5 14-15 0.0 0.0 0.0 1.4498857852472672E-4 3.624714463118168E-5 16-17 0.0 0.0 0.0 1.4498857852472672E-4 3.624714463118168E-5 18-19 0.0 0.0 0.0 1.9935929547149924E-4 5.437071694677252E-5 20-21 0.0 0.0 0.0 2.1748286778709006E-4 1.0874143389354503E-4 22-23 0.0 0.0 0.0 3.262243016806351E-4 1.6311215084031754E-4 24-25 0.0 0.0 0.0 4.349657355741801E-4 2.1748286778709006E-4 26-27 0.0 0.0 0.0 6.886957479924519E-4 2.1748286778709006E-4 28-29 0.0 0.0 0.0 0.0011961557728289954 2.1748286778709006E-4 30-31 0.0 0.0 0.0 0.003298490161437533 2.1748286778709006E-4 32-33 0.0 0.0 0.0 0.008735561856114784 2.1748286778709006E-4 34-35 0.0 0.0 0.0 0.015622519336039304 2.1748286778709006E-4 36-37 0.0 0.0 0.0 0.028780232837158255 2.1748286778709006E-4 38-39 0.0 0.0 0.0 0.0508366203452323 2.1748286778709006E-4 40-41 0.0 0.0 0.0 0.0745785000786563 2.1748286778709006E-4 42-43 0.0 0.0 0.0 0.09725108904546045 2.1748286778709006E-4 44-45 0.0 0.0 0.0 0.1264119169012461 2.1748286778709006E-4 46-47 0.0 0.0 0.0 0.16371022872673205 2.1748286778709006E-4 48-49 0.0 0.0 0.0 0.20369082925492543 2.1748286778709006E-4 50-51 0.0 0.0 0.0 0.2463718420581419 2.1748286778709006E-4 52-53 0.0 0.0 0.0 0.28834603554105026 2.1748286778709006E-4 54-55 0.0 0.0 0.0 0.33921890303091373 2.1748286778709006E-4 56-57 0.0 0.0 0.0 0.40094779033781613 2.1748286778709006E-4 58-59 0.0 0.0 0.0 0.47139411592851777 2.1748286778709006E-4 60-61 0.0 0.0 0.0 0.5501772847843911 2.1748286778709006E-4 62-63 0.0 0.0 0.0 0.6318239780661279 2.1748286778709006E-4 64-65 0.0 0.0 0.0 0.7177659579866595 2.1748286778709006E-4 66-67 0.0 0.0 0.0 0.8169925164145195 2.1748286778709006E-4 68-69 0.0 0.0 0.0 0.9314609991597912 2.1748286778709006E-4 70-71 0.0 0.0 0.0 1.0609176762100565 2.1748286778709006E-4 72-73 0.0 0.0 0.0 1.1964095028414137 2.5373001241827173E-4 74-75 0.0 0.0 0.0 1.339241376260585 2.718535847338626E-4 76-77 0.0 0.0 0.0 1.4986563183485222 2.8997715704945343E-4 78-79 0.0 1.812357231559084E-5 0.0 1.6835348795398644 2.8997715704945343E-4 80-81 0.0 3.624714463118168E-5 0.0 1.8898898739251815 2.8997715704945343E-4 82-83 0.0 3.624714463118168E-5 0.0 2.109529446817827 2.8997715704945343E-4 84-85 0.0 3.624714463118168E-5 0.0 2.333736159934001 2.8997715704945343E-4 86-87 0.0 3.624714463118168E-5 0.0 2.5759214567872415 3.081007293650443E-4 88-89 0.0 3.624714463118168E-5 0.0 2.8446577870828227 3.2622430168063513E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4005 0.0 59.820923 1 GTATCAA 7595 0.0 40.745373 1 ATCAACG 9420 0.0 32.14326 3 CAACGCA 9620 0.0 31.41567 5 TCAACGC 9705 0.0 31.247744 4 TATCAAC 9895 0.0 30.98512 2 AACGCAG 10185 0.0 29.858173 6 GTGGTAT 1890 0.0 28.421154 1 ACGCAGA 11595 0.0 25.777218 7 CGCAGAG 11670 0.0 25.529787 8 GCGCGTA 60 1.7263292E-4 23.720747 12-13 TGGTATC 2115 0.0 22.92446 2 GCAGAGT 13300 0.0 22.115585 9 TACATGG 6970 0.0 21.005219 2 GTACATG 6970 0.0 20.733177 1 GAGTACT 9675 0.0 20.594757 12-13 CAGAGTA 12850 0.0 20.342617 10-11 AGAGTAC 12165 0.0 20.181648 10-11 ACATGGG 7130 0.0 19.533474 3 GTACTTT 10615 0.0 18.793358 14-15 >>END_MODULE