FastQCFastQC Report
Sat 18 Jun 2016
SRR1930978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558636
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN694164.45363766780698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.051.4567871
GTATCAA23850.045.136241
ATCAACG28700.036.0044823
TCAACGC29550.035.1377034
CAACGCA30750.033.7599645
TATCAAC31000.033.4012572
AACGCAG34100.030.3136776
ACGCAGA38700.027.4677547
CGCAGAG39350.026.3529728
GAGTACA17050.021.9478971
GCAGAGT43800.021.9463399
GTACATG21050.020.699571
GTGGTAT7200.020.6471421
ACTATAC4550.019.940943
TAACGCA1807.820836E-519.6014944
CAGAGTA42600.019.45100610-11
TACATGG21400.019.4019052
GAGTACT32300.019.2518412-13
AGAGTAC39050.019.22630910-11
GTATAAC4007.2759576E-1219.2232021
Produced by FastQC (version 0.11.5)