##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930978_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558636 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.053206136647685 28.0 2.0 31.0 2.0 33.0 2 19.757188336468552 27.0 2.0 31.0 2.0 33.0 3 20.404736577366364 27.0 2.0 31.0 2.0 33.0 4 21.913924739323356 30.0 2.0 35.0 2.0 37.0 5 21.24821959713493 30.0 2.0 35.0 2.0 37.0 6 22.231033416397413 30.0 2.0 35.0 2.0 37.0 7 22.42442173798116 30.0 2.0 35.0 2.0 37.0 8 22.85236963601508 32.0 2.0 35.0 2.0 37.0 9 24.175745972760797 32.0 2.0 37.0 2.0 39.0 10-11 24.53672121008369 32.0 2.0 37.0 2.0 39.0 12-13 24.435227981388856 32.0 2.0 37.0 2.0 39.0 14-15 24.384741530415056 32.0 2.0 38.0 2.0 40.0 16-17 23.20297266327738 31.5 2.0 38.0 2.0 40.0 18-19 22.142029954396023 28.5 2.0 38.0 2.0 40.0 20-21 21.800265745177192 27.5 2.0 37.0 2.0 40.0 22-23 21.421226636623302 27.0 2.0 37.0 2.0 40.0 24-25 21.255142637536924 27.0 2.0 37.0 2.0 39.5 26-27 20.971159398345733 27.0 2.0 37.0 2.0 39.0 28-29 20.76626710790717 25.5 2.0 37.0 2.0 39.0 30-31 20.734507287140808 25.5 2.0 36.0 2.0 39.0 32-33 20.59118421491612 25.5 2.0 36.0 2.0 39.0 34-35 20.2565926232937 25.0 2.0 36.0 2.0 39.0 36-37 19.792445766683176 24.0 2.0 36.0 2.0 39.0 38-39 19.658626517031557 24.0 2.0 35.0 2.0 39.0 40-41 19.52189927603366 24.0 2.0 35.0 2.0 39.0 42-43 19.353374681452244 23.5 2.0 35.0 2.0 39.0 44-45 18.962572082256536 22.0 2.0 35.0 2.0 38.0 46-47 18.610214636387198 19.0 2.0 35.0 2.0 38.0 48-49 18.36053863762931 18.0 2.0 34.5 2.0 38.0 50-51 16.130868592795238 10.0 2.0 32.5 2.0 37.5 52-53 18.012030390674923 17.5 2.0 33.5 2.0 38.0 54-55 18.768162675570178 21.0 2.0 35.5 2.0 39.0 56-57 18.73594508275184 20.0 2.0 35.5 2.0 39.0 58-59 18.452531572477476 18.5 2.0 35.0 2.0 39.0 60-61 18.157196099666635 17.0 2.0 35.0 2.0 39.0 62-63 17.715215739916182 12.5 2.0 34.0 2.0 38.5 64-65 17.369181771754278 9.5 2.0 34.0 2.0 38.0 66-67 17.00896200267413 8.0 2.0 34.0 2.0 38.0 68-69 16.568151255328374 7.0 2.0 33.0 2.0 37.5 70-71 16.211543939701123 2.0 2.0 32.5 2.0 37.0 72-73 15.807370354592091 2.0 2.0 32.0 2.0 36.0 74-75 15.361674566736557 2.0 2.0 31.5 2.0 36.0 76-77 14.946445802612027 2.0 2.0 31.0 2.0 35.5 78-79 14.572693046997504 2.0 2.0 31.0 2.0 35.0 80-81 14.141052817976743 2.0 2.0 30.5 2.0 34.5 82-83 13.682448948952802 2.0 2.0 30.0 2.0 34.0 84-85 13.276973584595762 2.0 2.0 29.5 2.0 34.0 86-87 12.794253757772822 2.0 2.0 29.0 2.0 34.0 88-89 12.383878275620479 2.0 2.0 29.0 2.0 34.0 90-91 11.909627392155706 2.0 2.0 28.5 2.0 34.0 92-93 11.365651441388497 2.0 2.0 26.5 2.0 34.0 94-95 10.793868164215379 2.0 2.0 25.5 2.0 33.0 96-97 10.159471486607522 2.0 2.0 24.5 2.0 33.0 98-99 9.494129803238216 2.0 2.0 20.0 2.0 32.0 100-101 8.588210140148181 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.891845691587029 1101 2 9.612515128354662 1101 3 8.866277294554905 1101 4 10.73848616271859 1101 5 11.534287019677425 1101 6 10.774430900483484 1101 7 10.484513160053613 1101 8 10.016215173868797 1101 9 9.919423992770621 1101 10-11 9.22442706592022 1101 12-13 8.931824104891522 1101 14-15 9.691015392674576 1101 16-17 11.017549998248196 1101 18-19 11.805029684015214 1101 20-21 11.65650639176852 1101 22-23 11.75966812540997 1101 24-25 11.943696657814552 1101 26-27 11.952881269775975 1101 28-29 12.087133225181354 1101 30-31 11.500920778478875 1101 32-33 11.441840525373198 1101 34-35 11.282065900412054 1101 36-37 11.464818986123532 1101 38-39 11.24644514260494 1101 40-41 10.93613637998568 1101 42-43 10.742219674711023 1101 44-45 10.540587845101275 1101 46-47 10.557526876427655 1101 48-49 10.770768673254356 1101 50-51 12.301941836581609 1101 52-53 11.453970211280982 1101 54-55 11.23057928451443 1101 56-57 11.23172693437726 1101 58-59 11.086337461060229 1101 60-61 10.862253711855775 1101 62-63 10.827313651075578 1101 64-65 10.92197080050276 1101 66-67 10.66376592001313 1101 68-69 10.410047273657803 1101 70-71 10.156765988242267 1101 72-73 10.12121077216206 1101 74-75 9.890203322222199 1101 76-77 9.941964997972367 1101 78-79 9.48556618481886 1101 80-81 8.935687462747813 1101 82-83 8.710309792823054 1101 84-85 8.476473398854667 1101 86-87 8.226793460085336 1101 88-89 7.81029005669294 1101 90-91 7.536727805852324 1101 92-93 7.683346906796659 1101 94-95 7.248946416057732 1101 96-97 6.964038372977075 1101 98-99 6.734129478737396 1101 100-101 5.8850265345466575 1102 1 7.665598375336543 1102 2 8.894238867951753 1102 3 8.322623144508952 1102 4 10.358887578833095 1102 5 11.158396782326722 1102 6 10.118095283708154 1102 7 9.664201481044813 1102 8 9.269565063960524 1102 9 8.979955212821356 1102 10-11 8.104491653021295 1102 12-13 8.459553067345599 1102 14-15 8.94090745231826 1102 16-17 9.853758330023599 1102 18-19 10.808539664316339 1102 20-21 11.054199006758953 1102 22-23 11.252482269237301 1102 24-25 10.99454492929291 1102 26-27 10.879741395998298 1102 28-29 10.581370174434621 1102 30-31 10.499091204198795 1102 32-33 10.550597764855166 1102 34-35 10.082665131742896 1102 36-37 10.384518248744289 1102 38-39 10.375188764087532 1102 40-41 10.153053201953949 1102 42-43 9.522353532560363 1102 44-45 9.293304655655621 1102 46-47 9.291934168748245 1102 48-49 8.710007993850002 1102 50-51 10.568535231064743 1102 52-53 9.681641513708804 1102 54-55 9.428274851383332 1102 56-57 9.27402833691312 1102 58-59 9.284561610968424 1102 60-61 9.20078281048984 1102 62-63 9.116655992230076 1102 64-65 8.682331795561318 1102 66-67 8.454268349409116 1102 68-69 8.367213745828476 1102 70-71 8.15235745613272 1102 72-73 8.08815268509739 1102 74-75 7.40714612072107 1102 76-77 7.135215232646704 1102 78-79 7.024593363747956 1102 80-81 6.728553559636337 1102 82-83 6.676932706090959 1102 84-85 6.562292968234635 1102 86-87 6.542468131590461 1102 88-89 6.248073707615601 1102 90-91 6.241246738216914 1102 92-93 5.982837806155315 1102 94-95 5.487011282603307 1102 96-97 5.365799070092695 1102 98-99 5.04995489283675 1102 100-101 4.556674528942024 1103 1 8.026720237781198 1103 2 9.392576838272 1103 3 8.682403999444876 1103 4 10.570592973068056 1103 5 11.423122820639922 1103 6 10.72717983168921 1103 7 10.160519592007685 1103 8 9.479484724936828 1103 9 9.356902405812601 1103 10-11 8.949838273485053 1103 12-13 8.78678565132363 1103 14-15 9.351820384730576 1103 16-17 10.300923051584295 1103 18-19 11.324628339291639 1103 20-21 11.591998229020131 1103 22-23 11.613543607998412 1103 24-25 11.380878226198195 1103 26-27 11.477994481378058 1103 28-29 11.06650089041272 1103 30-31 10.937135443027145 1103 32-33 10.928067662386102 1103 34-35 10.997048784352891 1103 36-37 11.214849303806801 1103 38-39 10.960056439203989 1103 40-41 10.340868816836629 1103 42-43 9.81986633170673 1103 44-45 9.912603855908188 1103 46-47 9.999201602225316 1103 48-49 9.959347925213489 1103 50-51 11.558264608615659 1103 52-53 10.6709703724636 1103 54-55 10.334643005376837 1103 56-57 10.054356014792035 1103 58-59 9.985244105621849 1103 60-61 10.035089835183502 1103 62-63 10.022269402608732 1103 64-65 9.773101573335047 1103 66-67 9.303883122803892 1103 68-69 9.020666138164621 1103 70-71 8.947961387629775 1103 72-73 8.801900326760753 1103 74-75 8.76756541514014 1103 76-77 8.619023914057625 1103 78-79 8.099636660000572 1103 80-81 7.859092330462934 1103 82-83 7.325891546950476 1103 84-85 7.033010889164524 1103 86-87 6.834409146991934 1103 88-89 6.939692069173082 1103 90-91 6.714184113849283 1103 92-93 5.988900799356928 1103 94-95 5.964239768425964 1103 96-97 5.289289165458289 1103 98-99 5.2766010990832495 1103 100-101 4.787515462835742 1104 1 7.786122392803794 1104 2 9.419411084938517 1104 3 8.726910318823073 1104 4 10.56929359945931 1104 5 11.312297049113727 1104 6 10.824064145284193 1104 7 10.244878668764645 1104 8 10.005921098523377 1104 9 9.764675351922246 1104 10-11 8.993816190789577 1104 12-13 8.862522234200295 1104 14-15 9.559763055509091 1104 16-17 10.300816372789154 1104 18-19 11.491405567343914 1104 20-21 11.510207023642185 1104 22-23 11.576347097153043 1104 24-25 11.549998135382555 1104 26-27 11.563026725367433 1104 28-29 11.778950509179317 1104 30-31 11.310175474383687 1104 32-33 11.226111761218366 1104 34-35 11.145410516062196 1104 36-37 11.178249417696119 1104 38-39 11.130692407522048 1104 40-41 10.451471062083229 1104 42-43 10.243343431818406 1104 44-45 10.21010161879654 1104 46-47 10.274051475394028 1104 48-49 10.210080995270214 1104 50-51 11.626053713697047 1104 52-53 10.864622891710159 1104 54-55 10.618939960686369 1104 56-57 10.370448612725756 1104 58-59 10.428897364409448 1104 60-61 10.167648129506045 1104 62-63 9.985197689314003 1104 64-65 10.045797902992863 1104 66-67 9.827849799084593 1104 68-69 9.749333707071358 1104 70-71 9.593611222342354 1104 72-73 9.20167254812018 1104 74-75 9.011093506606578 1104 76-77 8.650131413743317 1104 78-79 8.55225426551459 1104 80-81 8.096304186784904 1104 82-83 7.817496277054975 1104 84-85 7.78552865663803 1104 86-87 7.175479373809171 1104 88-89 6.8055629896798155 1104 90-91 6.463767012702254 1104 92-93 6.129514914237236 1104 94-95 6.156581636062912 1104 96-97 5.778166837974725 1104 98-99 5.018158128790612 1104 100-101 4.494655176929177 1105 1 7.836083265771361 1105 2 9.040086067872473 1105 3 8.7187929858252 1105 4 10.871346149931338 1105 5 10.755020808564801 1105 6 10.091528769081936 1105 7 9.858772372734887 1105 8 9.556181112916203 1105 9 9.227391452464847 1105 10-11 8.82455301848449 1105 12-13 8.405722547956547 1105 14-15 9.260179670530544 1105 16-17 10.147956214468042 1105 18-19 10.7565525388376 1105 20-21 11.234448948951187 1105 22-23 11.210432384833855 1105 24-25 11.515569491804843 1105 26-27 11.587928722788572 1105 28-29 10.863226622079765 1105 30-31 10.62633151631898 1105 32-33 10.805862828193117 1105 34-35 10.732864335989046 1105 36-37 10.952345842243847 1105 38-39 10.585317444752853 1105 40-41 10.276693105679808 1105 42-43 10.41869784133321 1105 44-45 10.222473715949977 1105 46-47 10.259318436453846 1105 48-49 9.77160848501671 1105 50-51 11.315408200910923 1105 52-53 10.272578166644621 1105 54-55 9.967833211412024 1105 56-57 10.094543279794486 1105 58-59 9.98779455558801 1105 60-61 9.669260015244014 1105 62-63 9.678984538561153 1105 64-65 9.382909569508445 1105 66-67 8.98591283523131 1105 68-69 8.88697280228417 1105 70-71 8.823132429563923 1105 72-73 8.730612862283424 1105 74-75 8.441574112363334 1105 76-77 8.130483538535426 1105 78-79 8.072822319726344 1105 80-81 7.787446862629926 1105 82-83 7.865946458075314 1105 84-85 7.408609350823733 1105 86-87 7.005400916246739 1105 88-89 7.057246747058917 1105 90-91 6.545246884017795 1105 92-93 6.105267068998986 1105 94-95 5.86509042438289 1105 96-97 5.726170619329537 1105 98-99 4.940106081512576 1105 100-101 4.261499133143898 1106 1 7.874462347891026 1106 2 8.970220948105272 1106 3 8.497475918290881 1106 4 10.178588924782837 1106 5 10.950883436355856 1106 6 10.259674969922262 1106 7 9.507984412232634 1106 8 8.902627618225086 1106 9 8.664559578426658 1106 10-11 8.268723847644292 1106 12-13 7.992569682956745 1106 14-15 8.806570647310128 1106 16-17 10.083719384002645 1106 18-19 11.230302705349597 1106 20-21 11.041233217854984 1106 22-23 11.347592819965566 1106 24-25 11.024483127094644 1106 26-27 11.075201502916933 1106 28-29 11.133487727601633 1106 30-31 10.79445619019467 1106 32-33 10.771976625983896 1106 34-35 10.676341702501627 1106 36-37 10.742014886321503 1106 38-39 10.31644197658737 1106 40-41 10.374065270468169 1106 42-43 10.25801524152353 1106 44-45 9.705129974114588 1106 46-47 9.408681900668668 1106 48-49 9.316699196333857 1106 50-51 11.09975842880236 1106 52-53 10.17827626218902 1106 54-55 9.592916835390046 1106 56-57 9.567792070420651 1106 58-59 9.145822163092202 1106 60-61 9.112528735791631 1106 62-63 9.314694019477958 1106 64-65 9.044174501310287 1106 66-67 8.517759679525486 1106 68-69 8.3078980054656 1106 70-71 8.176461859831647 1106 72-73 7.97738584149022 1106 74-75 7.659667654936314 1106 76-77 7.425685564498785 1106 78-79 7.204872216654499 1106 80-81 7.034204202588901 1106 82-83 6.541065555547199 1106 84-85 6.5894904991640235 1106 86-87 6.476552575736523 1106 88-89 6.075772264055649 1106 90-91 5.56644450449704 1106 92-93 5.439083501763834 1106 94-95 5.466672490691829 1106 96-97 5.058356706507489 1106 98-99 5.056899248228431 1106 100-101 4.504533780149517 1107 1 7.237211981181517 1107 2 8.240118415833372 1107 3 8.080529400786908 1107 4 9.684939367322897 1107 5 10.873203236327164 1107 6 9.799212257179509 1107 7 9.513525312420306 1107 8 8.024220001207421 1107 9 5.208168731964488 1107 10-11 7.504165992448431 1107 12-13 7.4037308272660844 1107 14-15 7.911157883184121 1107 16-17 8.880279048591532 1107 18-19 10.386546209247985 1107 20-21 10.493104607486398 1107 22-23 10.183152516760483 1107 24-25 10.109157890160777 1107 26-27 10.238299766426628 1107 28-29 10.639041437003744 1107 30-31 10.163047557223312 1107 32-33 10.347414115553537 1107 34-35 10.40802964954321 1107 36-37 10.544356264329473 1107 38-39 10.714941029437462 1107 40-41 10.110860068316807 1107 42-43 9.610398194581787 1107 44-45 9.241944388360189 1107 46-47 9.25678291632958 1107 48-49 9.575357127360267 1107 50-51 10.951790409385055 1107 52-53 10.105272523362316 1107 54-55 9.88176645082083 1107 56-57 9.381703828840333 1107 58-59 9.559987983130789 1107 60-61 9.218181332329191 1107 62-63 9.32138335723425 1107 64-65 9.432716402812549 1107 66-67 9.339537759519398 1107 68-69 9.049277038172281 1107 70-71 8.87850123316096 1107 72-73 8.570364269309197 1107 74-75 8.439375151034994 1107 76-77 8.118645400657954 1107 78-79 8.072970611916114 1107 80-81 7.705162566054389 1107 82-83 7.742064820664268 1107 84-85 7.457728697851772 1107 86-87 6.833188919942806 1107 88-89 6.942332089674663 1107 90-91 6.610377182193782 1107 92-93 6.408683942108029 1107 94-95 6.104505493669423 1107 96-97 5.539973287925784 1107 98-99 5.306801166243604 1107 100-101 5.068362095686583 1108 1 6.726044347333822 1108 2 7.918523056001771 1108 3 7.878672298189034 1108 4 9.194999871253927 1108 5 10.883731035352888 1108 6 9.383942203749708 1108 7 8.276806557927085 1108 8 5.839053241561015 1108 9 7.0721398488141425 1108 10-11 6.167621503079754 1108 12-13 4.906124815981986 1108 14-15 7.174045881895825 1108 16-17 8.36504450958893 1108 18-19 8.057669672925496 1108 20-21 8.12414709251858 1108 22-23 8.884606307745859 1108 24-25 8.71406707675099 1108 26-27 7.704016841533171 1108 28-29 9.140838725829497 1108 30-31 9.578485836887204 1108 32-33 9.94457698730476 1108 34-35 9.662251840452665 1108 36-37 9.336718361952453 1108 38-39 8.807649500562938 1108 40-41 9.227821154856986 1108 42-43 8.453057060535674 1108 44-45 8.108478439075366 1108 46-47 8.696126575207039 1108 48-49 9.30034318039898 1108 50-51 10.825499237092636 1108 52-53 10.18571845228745 1108 54-55 9.433063230654387 1108 56-57 9.431964651394726 1108 58-59 9.317717043997053 1108 60-61 9.269849791491911 1108 62-63 9.385203645605653 1108 64-65 9.35143796970333 1108 66-67 8.70917249291676 1108 68-69 8.886061645328077 1108 70-71 8.921690083643782 1108 72-73 8.71580303783565 1108 74-75 8.58802172065765 1108 76-77 8.238604364959485 1108 78-79 8.082260438398665 1108 80-81 8.164935647556403 1108 82-83 7.736872093788904 1108 84-85 7.485605366454719 1108 86-87 7.229849125955241 1108 88-89 6.834767229115519 1108 90-91 6.544382893258755 1108 92-93 6.196685098124201 1108 94-95 5.991315119085671 1108 96-97 5.498404873048258 1108 98-99 5.098425656532491 1108 100-101 4.709007854372521 1109 1 6.685726788408189 1109 2 8.597681835464588 1109 3 8.13406860734306 1109 4 8.862046463616434 1109 5 10.066113275440593 1109 6 8.988029541590013 1109 7 7.680739318752956 1109 8 8.408857736353632 1109 9 7.657860294639104 1109 10-11 6.048281470191029 1109 12-13 6.483618329594265 1109 14-15 8.112590738464437 1109 16-17 6.662667202810194 1109 18-19 5.8910146391231955 1109 20-21 8.28638845922788 1109 22-23 9.272052498135043 1109 24-25 8.583744219086089 1109 26-27 9.244035843908467 1109 28-29 9.78920068453587 1109 30-31 7.394448562997201 1109 32-33 6.415841010692297 1109 34-35 8.033020523076456 1109 36-37 6.601575113277136 1109 38-39 6.5959166301079755 1109 40-41 7.722793016724335 1109 42-43 6.603080630789645 1109 44-45 7.0161161149386935 1109 46-47 6.580781426294195 1109 48-49 7.347863553137877 1109 50-51 8.958727082881051 1109 52-53 9.185056840739854 1109 54-55 9.291442181869442 1109 56-57 8.312833461919116 1109 58-59 8.171162503525647 1109 60-61 8.51102245730591 1109 62-63 8.871916417394434 1109 64-65 8.999179245055057 1109 66-67 8.466296373786488 1109 68-69 8.378816989746134 1109 70-71 7.836401028281095 1109 72-73 7.165358309167642 1109 74-75 6.293737415888785 1109 76-77 5.895168589333686 1109 78-79 7.073136192098646 1109 80-81 7.1215190570210485 1109 82-83 6.801826415002132 1109 84-85 6.541353767697183 1109 86-87 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60-61 -15.906516004232554 2313 62-63 -15.460698967220953 2313 64-65 -15.14143613956033 2313 66-67 -14.770934395444282 2313 68-69 -14.321183010253868 2313 70-71 -13.958060510180445 2313 72-73 -13.572795536986204 2313 74-75 -13.128724122572756 2313 76-77 -12.709139102974007 2313 78-79 -12.344402269439817 2313 80-81 -11.92740401990203 2313 82-83 -11.484327431151712 2313 84-85 -11.075261616918203 2313 86-87 -10.605067738652835 2313 88-89 -10.202643697250275 2313 90-91 -9.721947273012029 2313 92-93 -9.20088022254088 2313 94-95 -8.633387968800312 2313 96-97 -8.006939545003998 2313 98-99 -7.362085032303613 2313 100-101 -6.467654853465961 2314 1 -18.75673475005335 2314 2 -17.465087395304643 2314 3 -18.10470062342617 2314 4 -19.566876613306643 2314 5 -18.885271339944023 2314 6 -19.898124304563833 2314 7 -20.068799142785505 2314 8 -20.53268072518483 2314 9 -21.856601243822436 2314 10-11 -22.206487760578202 2314 12-13 -22.12653551655958 2314 14-15 -22.08248618461249 2314 16-17 -20.874563566420573 2314 18-19 -19.826831514722958 2314 20-21 -19.4545346176952 2314 22-23 -19.07471673263419 2314 24-25 -18.916871165529297 2314 26-27 -18.64396415609153 2314 28-29 -18.45387623854105 2314 30-31 -18.41201297546434 2314 32-33 -18.289389758538473 2314 34-35 -17.94790255384662 2314 36-37 -17.491963348261326 2314 38-39 -17.343775677584333 2314 40-41 -17.218437752506436 2314 42-43 -17.06030398459497 2314 44-45 -16.703576192753612 2314 46-47 -16.37275703524427 2314 48-49 -16.084751831477508 2314 50-51 -13.928657532503564 2314 52-53 -15.770635466952456 2314 54-55 -16.49686551043825 2314 56-57 -16.47332038423473 2314 58-59 -16.205145188782044 2314 60-61 -15.906516004232554 2314 62-63 -15.460698967220953 2314 64-65 -15.14143613956033 2314 66-67 -14.770934395444282 2314 68-69 -14.321183010253868 2314 70-71 -13.958060510180445 2314 72-73 -13.572795536986204 2314 74-75 -13.128724122572756 2314 76-77 -12.709139102974007 2314 78-79 -12.344402269439817 2314 80-81 -11.92740401990203 2314 82-83 -11.484327431151712 2314 84-85 -11.075261616918203 2314 86-87 -10.605067738652835 2314 88-89 -10.202643697250275 2314 90-91 -9.721947273012029 2314 92-93 -9.20088022254088 2314 94-95 -8.633387968800312 2314 96-97 -8.006939545003998 2314 98-99 -7.362085032303613 2314 100-101 -6.467654853465961 2315 1 -18.75673475005335 2315 2 -17.465087395304643 2315 3 -18.10470062342617 2315 4 -19.566876613306643 2315 5 -18.885271339944023 2315 6 -19.898124304563833 2315 7 -20.068799142785505 2315 8 -20.53268072518483 2315 9 -21.856601243822436 2315 10-11 -22.206487760578202 2315 12-13 -22.12653551655958 2315 14-15 -22.08248618461249 2315 16-17 -20.874563566420573 2315 18-19 -19.826831514722958 2315 20-21 -19.4545346176952 2315 22-23 -19.07471673263419 2315 24-25 -18.916871165529297 2315 26-27 -18.64396415609153 2315 28-29 -18.45387623854105 2315 30-31 -18.41201297546434 2315 32-33 -18.289389758538473 2315 34-35 -17.94790255384662 2315 36-37 -17.491963348261326 2315 38-39 -17.343775677584333 2315 40-41 -17.218437752506436 2315 42-43 -17.06030398459497 2315 44-45 -16.703576192753612 2315 46-47 -16.37275703524427 2315 48-49 -16.084751831477508 2315 50-51 -13.928657532503564 2315 52-53 -15.770635466952456 2315 54-55 -16.49686551043825 2315 56-57 -16.47332038423473 2315 58-59 -16.205145188782044 2315 60-61 -15.906516004232554 2315 62-63 -15.460698967220953 2315 64-65 -15.14143613956033 2315 66-67 -14.770934395444282 2315 68-69 -14.321183010253868 2315 70-71 -13.958060510180445 2315 72-73 -13.572795536986204 2315 74-75 -13.128724122572756 2315 76-77 -12.709139102974007 2315 78-79 -12.344402269439817 2315 80-81 -11.92740401990203 2315 82-83 -11.484327431151712 2315 84-85 -11.075261616918203 2315 86-87 -10.605067738652835 2315 88-89 -10.202643697250275 2315 90-91 -9.721947273012029 2315 92-93 -9.20088022254088 2315 94-95 -8.633387968800312 2315 96-97 -8.006939545003998 2315 98-99 -7.362085032303613 2315 100-101 -6.467654853465961 2316 1 -18.75673475005335 2316 2 -17.465087395304643 2316 3 -18.10470062342617 2316 4 -19.566876613306643 2316 5 -18.885271339944023 2316 6 -19.898124304563833 2316 7 -20.068799142785505 2316 8 -20.53268072518483 2316 9 -21.856601243822436 2316 10-11 -22.206487760578202 2316 12-13 -22.12653551655958 2316 14-15 -22.08248618461249 2316 16-17 -20.874563566420573 2316 18-19 -19.826831514722958 2316 20-21 -19.4545346176952 2316 22-23 -19.07471673263419 2316 24-25 -18.916871165529297 2316 26-27 -18.64396415609153 2316 28-29 -18.45387623854105 2316 30-31 -18.41201297546434 2316 32-33 -18.289389758538473 2316 34-35 -17.94790255384662 2316 36-37 -17.491963348261326 2316 38-39 -17.343775677584333 2316 40-41 -17.218437752506436 2316 42-43 -17.06030398459497 2316 44-45 -16.703576192753612 2316 46-47 -16.37275703524427 2316 48-49 -16.084751831477508 2316 50-51 -13.928657532503564 2316 52-53 -15.770635466952456 2316 54-55 -16.49686551043825 2316 56-57 -16.47332038423473 2316 58-59 -16.205145188782044 2316 60-61 -15.906516004232554 2316 62-63 -15.460698967220953 2316 64-65 -15.14143613956033 2316 66-67 -14.770934395444282 2316 68-69 -14.321183010253868 2316 70-71 -13.958060510180445 2316 72-73 -13.572795536986204 2316 74-75 -13.128724122572756 2316 76-77 -12.709139102974007 2316 78-79 -12.344402269439817 2316 80-81 -11.92740401990203 2316 82-83 -11.484327431151712 2316 84-85 -11.075261616918203 2316 86-87 -10.605067738652835 2316 88-89 -10.202643697250275 2316 90-91 -9.721947273012029 2316 92-93 -9.20088022254088 2316 94-95 -8.633387968800312 2316 96-97 -8.006939545003998 2316 98-99 -7.362085032303613 2316 100-101 -6.467654853465961 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 400769.0 3 17131.0 4 60819.0 5 33151.0 6 19019.0 7 18923.0 8 18851.0 9 18468.0 10 19579.0 11 20551.0 12 21401.0 13 21382.0 14 21902.0 15 22892.0 16 23539.0 17 23581.0 18 23366.0 19 23769.0 20 24893.0 21 26768.0 22 27876.0 23 29804.0 24 31758.0 25 33873.0 26 36502.0 27 38927.0 28 41916.0 29 44978.0 30 48855.0 31 52851.0 32 57453.0 33 61887.0 34 64546.0 35 60301.0 36 45258.0 37 18768.0 38 2315.0 39 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.05187807068519 20.570454065190976 14.332670769718423 23.04499709440541 2 14.829752072115895 20.895949062204767 41.833009194177656 22.44128967150168 3 18.518169537881693 26.292630455447526 30.61954872171172 24.569651284959058 4 12.12061722807228 17.505821277245488 39.81199228555796 30.561569209124272 5 12.423437332047262 37.906213763825036 36.08212217114081 13.588226732986893 6 29.305017650889397 38.833756040717006 18.387856873564797 13.473369434828802 7 25.654160613534245 33.3003648771918 23.416753944390013 17.62872056488395 8 24.1171926948272 36.92033498752339 21.129624386296022 17.832847931353385 9 25.086079506162058 17.21444534224338 21.77240069009194 35.92707446150262 10-11 23.59195257215288 27.37373649179856 29.51492901349273 19.519381922555826 12-13 24.37175954437299 24.97901907677859 29.630306444952097 21.018914933896323 14-15 21.520545449605997 26.618554427503238 27.415906123603495 24.444993999287266 16-17 21.005023282030457 29.576370854875133 28.20475512907154 21.213850734022866 18-19 20.897273114164825 28.373567180295233 30.365841648582393 20.363318056957553 20-21 21.384143860892358 27.648847866626664 30.563787611596588 20.40322066088439 22-23 21.004846565924236 27.65693497355252 30.361370074267196 20.976848386256044 24-25 20.984748460655716 27.74666967513798 30.46456202944894 20.804019834757362 26-27 21.009729267661715 27.942726669441104 30.175846671240734 20.87169739165645 28-29 20.999467083219162 28.124413413968462 30.209606741936668 20.66651276087571 30-31 21.12421951337215 27.906389862783108 30.221628760425855 20.74776186341888 32-33 20.92142397601857 28.021488311758603 30.147448437566837 20.909639274655994 34-35 21.16416582757307 28.043443504054867 30.108166535133073 20.684224133238992 36-37 21.21666499077076 27.88656827754962 29.989337758289153 20.907428973390466 38-39 21.190595563995508 28.023009721230085 29.97120457177338 20.815190143001026 40-41 21.364755704766612 27.986588046831063 29.887566250276933 20.761089998125396 42-43 21.16144230823104 28.084394244937943 29.683788793722066 21.07037465310895 44-45 21.257943406734185 28.29536238451488 29.625471416440973 20.821222792309957 46-47 21.328470780614218 28.3734291670782 29.165528020873428 21.13257203143415 48-49 21.3288190860731 28.460824596763278 29.084961469717225 21.125394847446394 50-51 21.53163321471832 28.707437547533555 28.76849599573309 20.99243324201504 52-53 21.128815949676998 28.79440785950946 28.933873622435787 21.142902568377767 54-55 21.071518280274557 28.750165610590052 29.047825830738244 21.130490278397147 56-57 21.04210484235991 28.881489782312382 29.040490335525988 21.03591503980172 58-59 21.05712006035674 28.75567015313139 29.029225504129514 21.157984282382355 60-61 21.05769881843112 28.91623218336324 29.08648474794326 20.93958425026238 62-63 21.04517561397649 28.857729054410964 29.07383425432094 21.0232610772916 64-65 20.777747908543812 28.703845992941012 29.17410003284695 21.344306065668224 66-67 20.763973687073957 28.813396095918193 28.943259782821222 21.479370434186627 68-69 20.97174351736581 28.770294469140662 28.914430453753763 21.343531559739763 70-71 20.948419866148885 28.63740526609141 28.9391127332477 21.475062134512005 72-73 20.88036518656198 28.740871989575545 28.851210122831272 21.5275527010312 74-75 20.942871895339753 28.929162012779436 28.576271903885463 21.551694187995345 76-77 20.985151951156663 28.890138247257063 28.587579816122393 21.537129985463878 78-79 20.89783678230363 28.908657952865134 28.594097550722203 21.59940771410903 80-81 20.97527732711835 28.97691617185634 28.30960165366846 21.738204847356847 82-83 21.12113688154804 28.898460646456016 28.462441681213825 21.517960790782123 84-85 21.064630681249245 28.935569584864368 28.411513218765094 21.588286515121293 86-87 21.029921421953816 28.988338292459346 28.38801443783772 21.593725847749113 88-89 21.114704012440004 28.936572339920946 28.235654599913595 21.71306904772546 90-91 21.04693015497751 28.949701212503548 28.312396771829253 21.690971860689693 92-93 21.079115779228943 28.818333684421955 28.30994919829322 21.792601338055885 94-95 20.950749243577366 28.83016849813304 28.29883755728122 21.920244701008375 96-97 20.964260405695704 28.774863593662896 28.277343039705755 21.983532960935644 98-99 20.8803629574203 28.73405009311767 28.01451165965947 22.37107528980257 100-101 21.085265253818346 28.802355804551773 28.07017897566212 22.042199965967768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 93922.0 1 85194.0 2 56477.5 3 28706.0 4 17956.5 5 14097.5 6 13119.0 7 13267.0 8 13694.5 9 13458.5 10 12338.5 11 10621.5 12 8596.0 13 6678.5 14 5144.5 15 4219.5 16 3964.0 17 4221.0 18 4770.0 19 5574.5 20 6434.5 21 7331.0 22 8509.0 23 9730.5 24 10972.5 25 12571.0 26 14545.0 27 16404.0 28 18327.5 29 20951.5 30 23557.5 31 26090.0 32 29072.0 33 31726.5 34 33691.5 35 35557.5 36 37971.5 37 40309.0 38 41832.5 39 42988.5 40 43778.0 41 44233.0 42 44550.5 43 45125.5 44 45464.0 45 45509.0 46 45191.5 47 44220.0 48 42859.5 49 41258.5 50 39150.5 51 36470.0 52 33491.5 53 30332.5 54 27298.5 55 23775.0 56 20140.0 57 16629.5 58 13441.0 59 10689.0 60 8052.0 61 5941.0 62 4413.0 63 3154.5 64 2081.0 65 1324.0 66 830.5 67 521.0 68 336.5 69 218.0 70 151.0 71 110.5 72 71.5 73 46.5 74 34.5 75 27.0 76 22.5 77 18.5 78 12.5 79 8.0 80 5.0 81 4.0 82 5.0 83 3.0 84 2.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.27460420521533 2 29.304019668479363 3 23.057083244580518 4 29.407892541940516 5 32.552372715630845 6 25.121708981442747 7 24.03871077018624 8 20.011279092745195 9 11.863321519585073 10-11 15.495567919642559 12-13 15.562292927919028 14-15 19.70427989601164 16-17 23.05352243885038 18-19 27.034407007152407 20-21 29.109266050572423 22-23 28.733424609722864 24-25 27.207250442053184 26-27 28.698073187068694 28-29 25.65823579078117 30-31 27.542832322620548 32-33 26.502852494103816 34-35 24.557626026859385 36-37 27.580942567732297 38-39 27.698641632812283 40-41 24.70467767971483 42-43 25.56783623629892 44-45 24.2531610972671 46-47 27.571318768461655 48-49 22.949392930742007 50-51 28.5473324111595 52-53 22.116645579853152 54-55 23.2450360443362 56-57 25.3704200339271 58-59 24.655211351463716 60-61 24.197824251460894 62-63 24.026809338421543 64-65 22.553213194100483 66-67 23.052303424276097 68-69 24.061519174457665 70-71 22.53091805912349 72-73 22.402408259529487 74-75 19.667613220790486 76-77 21.943000161679826 78-79 21.096939888466583 80-81 22.09406173089804 82-83 22.98352533882189 84-85 19.908240281887498 86-87 21.469412999571418 88-89 20.327741692094882 90-91 21.43563346413146 92-93 21.42090905124737 94-95 20.461416263964132 96-97 19.376204578875374 98-99 18.80060514449814 100-101 20.44319520401171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1558636.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.26772012080244 #Duplication Level Percentage of deduplicated Percentage of total 1 92.20265409689071 76.77504795735054 2 5.499984448307822 9.159423314209235 3 1.149690343740496 2.8719628130451826 4 0.4384120258578577 1.4602227946690436 5 0.24494959487538132 1.0198197154893598 6 0.1383046294290368 0.6909786704824998 7 0.08354764191638 0.48697751646923065 8 0.06471533704966892 0.4310958858380183 9 0.036670387648691176 0.27481136179072924 >10 0.13452044822552442 1.733075747973837 >50 0.004257964144964609 0.23213937642308416 >100 0.001987375281671655 0.24026339974738525 >500 7.642665792916889E-5 0.042956146544946804 >1k 1.5285331585833777E-4 0.16367882802015285 >5k 0.0 0.0 >10k+ 7.642665792916889E-5 4.417546471946707 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 69416 4.45363766780698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.415866180429555E-5 2 0.0 0.0 0.0 0.0 6.415866180429555E-5 3 0.0 0.0 0.0 0.0 6.415866180429555E-5 4 0.0 0.0 0.0 0.0 6.415866180429555E-5 5 0.0 0.0 0.0 0.0 6.415866180429555E-5 6 0.0 0.0 0.0 0.0 6.415866180429555E-5 7 0.0 0.0 0.0 0.0 6.415866180429555E-5 8 0.0 0.0 0.0 0.0 6.415866180429555E-5 9 0.0 0.0 0.0 0.0 6.415866180429555E-5 10-11 0.0 0.0 0.0 0.0 6.415866180429555E-5 12-13 0.0 0.0 0.0 0.0 1.283173236085911E-4 14-15 0.0 0.0 0.0 0.0 1.283173236085911E-4 16-17 0.0 0.0 0.0 0.0 1.283173236085911E-4 18-19 0.0 0.0 0.0 0.0 1.283173236085911E-4 20-21 0.0 0.0 0.0 0.0 1.283173236085911E-4 22-23 0.0 0.0 0.0 3.2079330902147774E-5 1.283173236085911E-4 24-25 0.0 0.0 0.0 6.415866180429555E-5 1.283173236085911E-4 26-27 0.0 0.0 0.0 1.603966545107389E-4 1.283173236085911E-4 28-29 0.0 0.0 0.0 4.811899635322166E-4 1.283173236085911E-4 30-31 0.0 0.0 0.0 0.00115485591247732 1.283173236085911E-4 32-33 0.0 0.0 0.0 0.0035928850610405507 1.283173236085911E-4 34-35 0.0 0.0 0.0 0.006704580158548886 1.283173236085911E-4 36-37 0.0 0.0 0.0 0.012222225073718302 1.283173236085911E-4 38-39 0.0 0.0 0.0 0.020979882410004647 1.283173236085911E-4 40-41 0.0 0.0 0.0 0.03002625372441032 1.283173236085911E-4 42-43 0.0 0.0 0.0 0.038848069722500954 1.9247598541288665E-4 44-45 0.0 0.0 0.0 0.051391088105240734 1.9247598541288665E-4 46-47 0.0 3.2079330902147774E-5 0.0 0.06573054901850078 1.9247598541288665E-4 48-49 0.0 6.415866180429555E-5 0.0 0.08109654852062959 1.9247598541288665E-4 50-51 0.0 6.415866180429555E-5 0.0 0.09864394252410441 1.9247598541288665E-4 52-53 0.0 6.415866180429555E-5 0.0 0.11978422158861979 1.9247598541288665E-4 54-55 0.0 6.415866180429555E-5 0.0 0.14211143589651465 1.9247598541288665E-4 56-57 0.0 6.415866180429555E-5 0.0 0.17005253311228535 1.9247598541288665E-4 58-59 0.0 6.415866180429555E-5 0.0 0.20120156341827083 1.9247598541288665E-4 60-61 0.0 6.415866180429555E-5 0.0 0.2350131781891346 1.9247598541288665E-4 62-63 0.0 6.415866180429555E-5 0.0 0.2678944923638361 2.2455531631503442E-4 64-65 0.0 6.415866180429555E-5 0.0 0.3041120569523609 2.566346472171822E-4 66-67 0.0 6.415866180429555E-5 0.0 0.3461680597650767 2.566346472171822E-4 68-69 0.0 6.415866180429555E-5 0.0 0.3926830895731909 2.566346472171822E-4 70-71 0.0 6.415866180429555E-5 0.0 0.44333635306768227 2.566346472171822E-4 72-73 0.0 6.415866180429555E-5 0.0 0.4967163596888562 2.566346472171822E-4 74-75 0.0 6.415866180429555E-5 0.0 0.5532722200693427 2.566346472171822E-4 76-77 0.0 6.415866180429555E-5 0.0 0.6172063265573232 2.566346472171822E-4 78-79 0.0 6.415866180429555E-5 0.0 0.6901868043597095 2.566346472171822E-4 80-81 0.0 6.415866180429555E-5 0.0 0.769775624327938 2.566346472171822E-4 82-83 0.0 6.415866180429555E-5 0.0 0.85270069470999 2.566346472171822E-4 84-85 0.0 6.415866180429555E-5 0.0 0.9383845875496267 2.566346472171822E-4 86-87 0.0 6.415866180429555E-5 0.0 1.0325053444165282 2.566346472171822E-4 88-89 0.0 6.415866180429555E-5 0.0 1.1350308859797926 2.566346472171822E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1315 0.0 51.456787 1 GTATCAA 2385 0.0 45.13624 1 ATCAACG 2870 0.0 36.004482 3 TCAACGC 2955 0.0 35.137703 4 CAACGCA 3075 0.0 33.759964 5 TATCAAC 3100 0.0 33.401257 2 AACGCAG 3410 0.0 30.313677 6 ACGCAGA 3870 0.0 27.467754 7 CGCAGAG 3935 0.0 26.352972 8 GAGTACA 1705 0.0 21.947897 1 GCAGAGT 4380 0.0 21.946339 9 GTACATG 2105 0.0 20.69957 1 GTGGTAT 720 0.0 20.647142 1 ACTATAC 455 0.0 19.94094 3 TAACGCA 180 7.820836E-5 19.601494 4 CAGAGTA 4260 0.0 19.451006 10-11 TACATGG 2140 0.0 19.401905 2 GAGTACT 3230 0.0 19.25184 12-13 AGAGTAC 3905 0.0 19.226309 10-11 GTATAAC 400 7.2759576E-12 19.223202 1 >>END_MODULE