FastQCFastQC Report
Sat 18 Jun 2016
SRR1930978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558636
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47680.3059084994828812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.28281138123333477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.16501607816064814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.059.5370941
GTATCAA43350.042.334461
TCAACGC55850.032.4285744
ATCAACG56000.032.4265943
CAACGCA56250.032.1359945
ATCACGC1805.456968E-1231.6909143
TATCAAC57800.031.4187892
AACGCAG59250.030.8291576
TAACGCA1901.2732926E-1130.022974
GTGGTAT11000.027.661971
TCACGCA2104.1836756E-1127.163644
CGCAGAG67900.026.7619828
ACGCAGA68200.026.644267
TATCACG2059.476935E-1025.5089592
TGGTATC11400.025.4377632
ATAACGC2101.2551027E-924.9000023
GCAGAGT77050.023.2144289
GGCGTCG1050.002211443822.5927968
AGAGTAC72200.021.783910-11
CAGAGTA74850.020.06185710-11
Produced by FastQC (version 0.11.5)