##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930978_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558636 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34967433063268 31.0 31.0 34.0 30.0 34.0 2 31.50441154958566 31.0 31.0 34.0 30.0 34.0 3 31.56617003585186 31.0 31.0 34.0 30.0 34.0 4 35.3348966660593 37.0 35.0 37.0 33.0 37.0 5 35.21292591727639 37.0 35.0 37.0 33.0 37.0 6 35.24434056444224 37.0 35.0 37.0 32.0 37.0 7 35.128192214218075 37.0 35.0 37.0 32.0 37.0 8 35.13071621597345 37.0 35.0 37.0 32.0 37.0 9 36.64105795066969 39.0 35.0 39.0 32.0 39.0 10-11 36.58125758676176 38.5 35.0 39.0 32.0 39.0 12-13 36.4713111335809 38.0 35.0 39.0 32.0 39.0 14-15 37.525943517280496 39.0 36.0 41.0 32.0 41.0 16-17 37.48044123194896 39.0 36.0 41.0 32.0 41.0 18-19 37.51364430181261 39.0 36.0 41.0 32.0 41.0 20-21 37.49685558398497 39.0 36.0 41.0 32.0 41.0 22-23 37.39180668225295 39.0 36.0 41.0 32.0 41.0 24-25 37.26281954221511 39.0 36.0 41.0 31.0 41.0 26-27 37.003648703096815 39.0 36.0 40.0 31.0 41.0 28-29 36.810369130444826 39.0 36.0 40.0 30.5 41.0 30-31 36.56959097569927 38.5 35.0 40.0 30.0 41.0 32-33 36.38387153896099 38.0 35.0 40.0 30.0 41.0 34-35 36.43826204450558 38.0 35.0 40.0 30.0 41.0 36-37 36.48882356111369 38.5 35.0 40.0 30.0 41.0 38-39 36.463091767417154 39.0 35.0 40.0 30.0 41.0 40-41 36.343223818774874 39.0 35.0 40.0 30.0 41.0 42-43 36.18951634634385 38.5 35.0 40.0 30.0 41.0 44-45 35.999580402351796 38.0 35.0 40.0 29.5 41.0 46-47 35.73347850299878 38.0 35.0 40.0 28.5 41.0 48-49 35.49317415997064 38.0 34.5 40.0 27.0 41.0 50-51 35.22552379131497 38.0 34.0 40.0 26.5 41.0 52-53 35.01181834629766 38.0 34.0 40.0 26.0 41.0 54-55 34.85470918161778 38.0 34.0 40.0 26.0 41.0 56-57 34.679875865821145 38.0 33.5 40.0 26.0 41.0 58-59 34.465848665114876 38.0 33.0 40.0 25.0 41.0 60-61 34.16303806661722 37.5 33.0 40.0 24.5 41.0 62-63 33.903727361616184 37.0 33.0 40.0 23.5 41.0 64-65 33.587472636330745 37.0 32.0 39.5 23.0 41.0 66-67 33.26366611575762 36.0 32.0 39.0 22.5 41.0 68-69 32.913620948059716 36.0 31.5 39.0 22.0 40.0 70-71 32.532678252010086 35.0 31.0 39.0 21.5 40.0 72-73 32.1033233545228 35.0 31.0 38.0 20.5 40.0 74-75 31.589691563649243 35.0 30.5 37.0 19.0 39.5 76-77 30.217872229308192 33.5 28.5 36.0 18.0 38.5 78-79 30.940774497701838 34.0 30.0 36.0 19.0 39.0 80-81 30.899909921238827 34.0 30.0 36.0 18.5 38.5 82-83 30.620933303221534 34.0 30.0 36.0 18.0 37.0 84-85 30.278762648880175 34.0 30.0 35.0 17.5 37.0 86-87 29.92779744597199 34.0 30.0 35.0 13.0 36.5 88-89 29.566536381810764 34.0 29.0 35.0 7.5 36.0 90-91 29.227194162075044 34.0 29.0 35.0 4.5 36.0 92-93 28.904677551397505 34.0 29.0 35.0 2.0 35.0 94-95 28.515099099469023 34.0 29.0 35.0 2.0 35.0 96-97 28.0747730708132 34.0 29.0 35.0 2.0 35.0 98-99 27.567610397809368 33.0 27.5 35.0 2.0 35.0 100-101 26.426452359627262 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33375890564368405 1101 2 0.42519675220564324 1101 3 0.31217364972338757 1101 4 0.11163648865648668 1101 5 0.33439898515864286 1101 6 0.38785311491778884 1101 7 0.48523876537709754 1101 8 0.471046038885369 1101 9 0.5473727449872783 1101 10-11 0.44249413770198487 1101 12-13 0.5124084255915164 1101 14-15 0.49536948866174413 1101 16-17 0.58623785925748 1101 18-19 0.6578321192636238 1101 20-21 0.5071719789509075 1101 22-23 0.5135867303707329 1101 24-25 0.5456698617761333 1101 26-27 0.6785304508175543 1101 28-29 0.6669880621632132 1101 30-31 0.5914555561547914 1101 32-33 0.5901865698466153 1101 34-35 0.6252792234859044 1101 36-37 0.7402258868376776 1101 38-39 0.692064371240825 1101 40-41 0.75862190774113 1101 42-43 0.606962335381624 1101 44-45 0.6769152425779055 1101 46-47 0.4124057581165701 1101 48-49 0.456414072550416 1101 50-51 0.5271603122650887 1101 52-53 0.35761276857946456 1101 54-55 0.2810998892230643 1101 56-57 0.4647477187345075 1101 58-59 0.48288070564252905 1101 60-61 0.38403879647256645 1101 62-63 0.1264001232953973 1101 64-65 0.313017883335057 1101 66-67 0.3151226349719636 1101 68-69 0.30532314840592534 1101 70-71 0.3127435111820134 1101 72-73 0.41694852552118533 1101 74-75 0.28275448761298705 1101 76-77 0.5057247897109569 1101 78-79 0.40816064003704255 1101 80-81 0.2680948672089052 1101 82-83 0.3482348960041648 1101 84-85 0.4829536403317469 1101 86-87 0.3776634411326718 1101 88-89 0.5666061034418632 1101 90-91 0.3372660420953828 1101 92-93 0.18474436992257992 1101 94-95 0.2836740008209411 1101 96-97 0.5388927626167899 1101 98-99 0.8241024751456862 1101 100-101 0.9933480348466759 1102 1 0.2544238261102372 1102 2 0.28015561285926793 1102 3 0.3823110695060912 1102 4 0.23837637502884235 1102 5 0.1348551487792946 1102 6 0.2998477779215065 1102 7 0.3332126943863898 1102 8 0.4356691528810117 1102 9 0.4668006315800426 1102 10-11 0.4402611400300387 1102 12-13 0.38243524832726905 1102 14-15 0.45047141702555393 1102 16-17 0.514260238432179 1102 18-19 0.4661259138246905 1102 20-21 0.3985544617168557 1102 22-23 0.4617690620005064 1102 24-25 0.39483261064408737 1102 26-27 0.42531674071956616 1102 28-29 0.3707286825693501 1102 30-31 0.49385563014854483 1102 32-33 0.5768413240436914 1102 34-35 0.36467440326514833 1102 36-37 0.3714727012688215 1102 38-39 0.41010113590329667 1102 40-41 0.5546760333848866 1102 42-43 0.5998469307086012 1102 44-45 0.6409822226684767 1102 46-47 0.7416897701523624 1102 48-49 0.5468832441155556 1102 50-51 0.4364585326759496 1102 52-53 0.5636956061612111 1102 54-55 0.44202075723276835 1102 56-57 0.34963429772383137 1102 58-59 0.6300759291095659 1102 60-61 0.4473579673489354 1102 62-63 0.27422685196462737 1102 64-65 0.35739780190257164 1102 66-67 0.30729346608319474 1102 68-69 0.34341025243602274 1102 70-71 0.3757387625934996 1102 72-73 0.2570745904113423 1102 74-75 0.24852450479291832 1102 76-77 0.28054841497195326 1102 78-79 0.3302127465959188 1102 80-81 0.3049551705439377 1102 82-83 0.25086757802191073 1102 84-85 0.4081951531891441 1102 86-87 0.31310271016883817 1102 88-89 0.4332703430084486 1102 90-91 0.21148315472528978 1102 92-93 0.3319122393157343 1102 94-95 0.6504208929848829 1102 96-97 0.7068526800429105 1102 98-99 0.6781466470552147 1102 100-101 0.978357805564432 1103 1 0.3484157785473485 1103 2 0.41723663339102757 1103 3 0.32614319388358837 1103 4 0.20185270362804886 1103 5 0.38528241942299246 1103 6 0.2337817186925335 1103 7 0.2558805024652173 1103 8 0.29215175165793994 1103 9 0.5289127297900578 1103 10-11 0.4724451305101738 1103 12-13 0.4875546412538583 1103 14-15 0.5589905713484313 1103 16-17 0.6842397704228276 1103 18-19 0.628820974636767 1103 20-21 0.6472362217379839 1103 22-23 0.38697279344686564 1103 24-25 0.6096319224575524 1103 26-27 0.6800446080243319 1103 28-29 0.7490770272940424 1103 30-31 0.5241330297323543 1103 32-33 0.7744879583197459 1103 34-35 0.9285293558859209 1103 36-37 0.9243273321085042 1103 38-39 0.885402540052759 1103 40-41 0.8637486663586458 1103 42-43 0.9925579819158159 1103 44-45 0.7684454796698645 1103 46-47 0.7727630846341853 1103 48-49 0.6929409324827205 1103 50-51 0.7148697537289337 1103 52-53 0.8358398748909153 1103 54-55 1.0406265495959346 1103 56-57 0.9676173450210896 1103 58-59 1.0632498138421198 1103 60-61 0.9021192649767187 1103 62-63 0.8841558855740601 1103 64-65 0.7405597716278365 1103 66-67 0.6839905957048131 1103 68-69 0.6000705138376219 1103 70-71 0.755342926319365 1103 72-73 0.7402150448208786 1103 74-75 0.6054305796252386 1103 76-77 0.5933327245010744 1103 78-79 0.6784506536224342 1103 80-81 0.6199513455989241 1103 82-83 0.7703013723218355 1103 84-85 0.7299990094015705 1103 86-87 0.7332000641265211 1103 88-89 0.6317927682664433 1103 90-91 0.5783221336471094 1103 92-93 0.41423867864102704 1103 94-95 0.4000169590592897 1103 96-97 0.5654926920034526 1103 98-99 0.645673698675278 1103 100-101 1.0747473686251787 1104 1 0.2513391842817505 1104 2 0.31030121751012274 1104 3 0.28305568399222736 1104 4 0.09393443770387933 1104 5 0.20437108299837803 1104 6 0.24809109829155318 1104 7 0.306114567204645 1104 8 0.31074381342440205 1104 9 0.44735836243044247 1104 10-11 0.38701390412926884 1104 12-13 0.553245298631893 1104 14-15 0.5191352255760151 1104 16-17 0.5224483431825817 1104 18-19 0.5032119625896385 1104 20-21 0.48850150250474655 1104 22-23 0.5165984377719965 1104 24-25 0.4498398444211844 1104 26-27 0.40699736614264737 1104 28-29 0.6472235525604546 1104 30-31 0.45963654625000316 1104 32-33 0.5427854978667099 1104 34-35 0.5328789665175577 1104 36-37 0.5110235434397552 1104 38-39 0.5462952400431433 1104 40-41 0.4043661091736368 1104 42-43 0.4021882853495029 1104 44-45 0.4962655345438094 1104 46-47 0.5274863483767049 1104 48-49 0.42231494796870805 1104 50-51 0.30792028656825465 1104 52-53 0.1863884294579563 1104 54-55 0.46409107750991296 1104 56-57 0.41433877470183234 1104 58-59 0.5781267911899732 1104 60-61 0.5023784741115307 1104 62-63 0.24781095623720262 1104 64-65 0.3275893310832316 1104 66-67 0.34116081586617497 1104 68-69 0.1468705197539535 1104 70-71 0.34695098435854277 1104 72-73 0.18832052555953283 1104 74-75 0.22688688384284106 1104 76-77 0.3654862310349216 1104 78-79 0.3042083134187621 1104 80-81 0.3761740288017279 1104 82-83 0.3840407506636332 1104 84-85 0.4479225260671278 1104 86-87 0.463801532855026 1104 88-89 0.7623766728952326 1104 90-91 0.5526055183785452 1104 92-93 0.4736199416292166 1104 94-95 0.6040291540910552 1104 96-97 0.6107058277314188 1104 98-99 0.6519298461101783 1104 100-101 0.9683645747870351 1105 1 0.18876331206342556 1105 2 0.24554385136064738 1105 3 0.29137688455388044 1105 4 0.1346132315922901 1105 5 0.19635919874039587 1105 6 0.222498134127477 1105 7 0.20254207489592346 1105 8 0.3021432662009431 1105 9 0.4924896831111596 1105 10-11 0.4207314162050011 1105 12-13 0.33444378830993315 1105 14-15 0.397129368546004 1105 16-17 0.4375245530218663 1105 18-19 0.2414970700826089 1105 20-21 0.2818184573671303 1105 22-23 0.30983695357173247 1105 24-25 0.2814870462371175 1105 26-27 0.29365551057782113 1105 28-29 0.378747730846527 1105 30-31 0.15596304514834003 1105 32-33 0.39052602736737896 1105 34-35 0.17615253939835895 1105 36-37 0.29005145405621846 1105 38-39 0.41786237481588273 1105 40-41 0.3620349150314084 1105 42-43 0.4135306848078315 1105 44-45 0.539435138667038 1105 46-47 0.4556481918426769 1105 48-49 0.3674264738859776 1105 50-51 0.44885141744124013 1105 52-53 0.32205267327024245 1105 54-55 0.45748647143495447 1105 56-57 0.4984409896803683 1105 58-59 0.5443985492424659 1105 60-61 0.655359435989034 1105 62-63 0.42724012705861014 1105 64-65 0.596820498770775 1105 66-67 0.7585219334008215 1105 68-69 0.573603070299967 1105 70-71 0.6307566478808653 1105 72-73 0.686877542803181 1105 74-75 0.5784703724201528 1105 76-77 0.5369160974480174 1105 78-79 0.7789550051957974 1105 80-81 0.77607653496678 1105 82-83 0.7501545206040845 1105 84-85 0.6664054701959436 1105 86-87 0.6359149660995023 1105 88-89 0.5899117438511325 1105 90-91 0.6062003029208718 1105 92-93 0.5799829405913783 1105 94-95 0.7611855160725263 1105 96-97 0.5746980430244442 1105 98-99 0.7602255169769165 1105 100-101 0.8897197945921569 1106 1 0.17374227451524504 1106 2 0.17957787206038844 1106 3 0.27911203444411115 1106 4 0.06808276744149566 1106 5 0.08843146163284388 1106 6 0.20136895835631918 1106 7 0.27060260945645354 1106 8 0.2581973478238382 1106 9 0.301845412338011 1106 10-11 0.14333770973270532 1106 12-13 0.11186619740174564 1106 14-15 0.2496674528743057 1106 16-17 0.31333954182417045 1106 18-19 0.33573255373278954 1106 20-21 0.24446346999260982 1106 22-23 0.11601865145679113 1106 24-25 0.2631508561345228 1106 26-27 0.21684803671008268 1106 28-29 0.39509295270674016 1106 30-31 0.37058577865334996 1106 32-33 0.40175187944880264 1106 34-35 0.3393824966903338 1106 36-37 0.1943712718902617 1106 38-39 0.3743293325810768 1106 40-41 0.4134203315984166 1106 42-43 0.42509636854470756 1106 44-45 0.22227855263020757 1106 46-47 0.26985963152926473 1106 48-49 0.45727137670552764 1106 50-51 0.33740981733644304 1106 52-53 0.46251685350502925 1106 54-55 0.4674654154876592 1106 56-57 0.42594111663425593 1106 58-59 0.44186304079028105 1106 60-61 0.38323413212764734 1106 62-63 0.34825861375347245 1106 64-65 0.35400369728765924 1106 66-67 0.3384344419288112 1106 68-69 0.2738069692932399 1106 70-71 0.5013843741697386 1106 72-73 0.4360041368105172 1106 74-75 0.5476652860215871 1106 76-77 0.39800333059645254 1106 78-79 0.41406945873104206 1106 80-81 0.22355662896417883 1106 82-83 0.330163992468524 1106 84-85 0.3940509133607115 1106 86-87 0.4613706569825915 1106 88-89 0.5529567532383624 1106 90-91 0.6015758693376263 1106 92-93 0.6045783256517367 1106 94-95 0.6170669036013123 1106 96-97 0.4235677117888592 1106 98-99 0.6205307877346087 1106 100-101 0.48103699460010674 1107 1 0.15015428911921447 1107 2 0.03087460373442852 1107 3 0.004090148986300335 1107 4 -0.06522016535624431 1107 5 0.0191848483891377 1107 6 0.1068515949171811 1107 7 0.13607438380347503 1107 8 0.14364783691623728 1107 9 0.1885643796371852 1107 10-11 0.19140703173732732 1107 12-13 0.09953348574228471 1107 14-15 0.2360508919507538 1107 16-17 0.2501143572982869 1107 18-19 0.21325523702166294 1107 20-21 0.18903470532788447 1107 22-23 0.11238046857926776 1107 24-25 0.20868534439542685 1107 26-27 0.23524938819200258 1107 28-29 0.333283003387308 1107 30-31 0.3717276286259761 1107 32-33 0.28556278909333344 1107 34-35 0.257064995058073 1107 36-37 0.26023661850027935 1107 38-39 0.2527439031722949 1107 40-41 0.25238398563543285 1107 42-43 0.23422553227135268 1107 44-45 0.3008982170434251 1107 46-47 0.36607915657823753 1107 48-49 0.2937654141991928 1107 50-51 0.2385047489864789 1107 52-53 0.446747246623481 1107 54-55 0.4811793264409303 1107 56-57 0.3858098478047154 1107 58-59 0.41118021450665765 1107 60-61 0.3760364515470087 1107 62-63 0.36853889400445183 1107 64-65 0.2716184898076648 1107 66-67 0.2008550613970641 1107 68-69 0.25405845443016517 1107 70-71 0.06621583418596089 1107 72-73 0.19425144101983705 1107 74-75 0.3732059812618296 1107 76-77 0.10425290972226264 1107 78-79 0.20597535272637657 1107 80-81 0.23913446360673518 1107 82-83 0.42545283659429245 1107 84-85 0.46781588550249964 1107 86-87 0.5178340401962238 1107 88-89 0.4353546235417731 1107 90-91 0.5323592662534615 1107 92-93 0.5555567300481528 1107 94-95 0.5012884400629254 1107 96-97 0.462590068625758 1107 98-99 0.6004744857474975 1107 100-101 0.8880055000356073 1108 1 0.06834457743309841 1108 2 0.05242050217754368 1108 3 0.10039607342540435 1108 4 0.1163290113416906 1108 5 0.03850929784362478 1108 6 0.1307871685830193 1108 7 0.06312602494384834 1108 8 0.030487656784764283 1108 9 0.08278602417083647 1108 10-11 0.03843402845331667 1108 12-13 0.07748650317455485 1108 14-15 0.14807504176124553 1108 16-17 0.044467850940201004 1108 18-19 -0.011364888012117547 1108 20-21 -0.1073626651666757 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-1.0024180451425195 2316 84-85 -0.8564420789668539 2316 86-87 -0.7929537009143104 2316 88-89 -0.7214080339112279 2316 90-91 -0.8009513956612757 2316 92-93 -0.8682707041820628 2316 94-95 -1.2013668364830394 2316 96-97 -0.8939712666301141 2316 98-99 -0.7403313471550774 2316 100-101 -0.9971796347686457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 15.0 5 36.0 6 74.0 7 225.0 8 608.0 9 1035.0 10 1992.0 11 3267.0 12 4691.0 13 6084.0 14 7247.0 15 9148.0 16 11130.0 17 12798.0 18 11004.0 19 8551.0 20 8110.0 21 8489.0 22 9562.0 23 10979.0 24 13258.0 25 16625.0 26 21485.0 27 27183.0 28 34723.0 29 42961.0 30 52784.0 31 63420.0 32 76711.0 33 92725.0 34 114249.0 35 143239.0 36 188641.0 37 259192.0 38 249226.0 39 47144.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.60958098124791 21.504722991814166 15.094004283479604 22.791691743458326 2 15.213805183680934 21.925257211052614 40.50547144933255 22.3554661559339 3 18.41504015460018 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20.611280615438112 27.702221727485586 31.00457806040499 20.681919596671307 24-25 20.59117434484785 27.87935403382401 31.03225820939141 20.497213411936727 26-27 20.55636166138572 28.021632365910097 30.854352057195268 20.567653915508917 28-29 20.506719670377823 28.183799521760417 30.830445653338533 20.479035154523224 30-31 20.813247620031454 27.903811863622686 30.846458423295296 20.43648209305056 32-33 20.58910256513097 28.12303772855399 30.71701418691172 20.570845519403324 34-35 20.82927074981576 28.087026073364243 30.702960897493277 20.380742279326718 36-37 20.80610179039275 27.970912814471994 30.744608467273547 20.478376927861714 38-39 20.68664081278994 28.13978159686879 30.65394225639608 20.519635333945192 40-41 20.882644018317574 28.127934300539742 30.544891690526025 20.444529990616655 42-43 20.75134466601111 28.12895318974188 30.37730855051982 20.74239359372719 44-45 20.725562140466383 28.37023617454787 30.312419495428223 20.591782189557527 46-47 20.913182590640194 28.425966652839126 29.922613874320774 20.73823688219991 48-49 20.889333338466535 28.57585137332353 29.74256359714132 20.792251691068618 50-51 20.92134805460764 28.719179715546105 29.551702616032237 20.807769613814017 52-53 21.10548825940213 28.68612508081266 29.446414423048207 20.76197223673701 54-55 20.930809336648753 28.68763936793134 29.61610652121601 20.765444774203896 56-57 20.893107203983195 28.78988288775286 29.459159890859922 20.857850017404026 58-59 20.911644274694538 28.760160449597688 29.54222710766563 20.78596816804214 60-61 20.910884576385453 28.801928646379178 29.611119907987177 20.676066869248196 62-63 20.904178436892565 28.7792674672296 29.653059026108597 20.663495069769237 64-65 20.721203953192745 28.6893641132602 29.554466700689364 21.03496523285769 66-67 20.656336322274328 28.83428085153461 29.511882061562044 20.997500764629017 68-69 20.822391038541962 28.835977815040003 29.503996470165244 20.83763467625279 70-71 20.823630492676003 28.643911053488942 29.460171567445276 21.072286886389787 72-73 20.80436853663983 28.793077688042914 29.357298807456328 21.045254967860924 74-75 20.842564146108007 28.986521083562334 29.16090946984901 21.010005300480643 76-77 20.78524315561786 28.909781674745226 29.201850648797507 21.10312452083941 78-79 20.744636824730758 28.956558841740215 29.230371917397317 21.068432416131714 80-81 20.8271593967929 29.021891263223505 29.064749263057333 21.08620007692626 82-83 20.878864596993782 29.020053431333547 29.16136288395751 20.939719087715154 84-85 20.843705650325028 29.05681634454741 29.025923948888643 21.073554056238915 86-87 20.786315727341083 29.149333134869202 28.99455036326634 21.069800774523365 88-89 20.84654869374443 28.99897313962959 28.947004573554608 21.20747359307137 90-91 20.71643974488945 29.04365624238069 29.11673745941715 21.123166553312718 92-93 20.788101283855458 28.921431793695014 29.128798160622143 21.16166876182739 94-95 20.61882194200465 28.921034742608786 29.145436213039922 21.314707102346638 96-97 20.66929032821005 28.81365501630913 29.133197231425427 21.383857424055392 98-99 20.597945896283672 28.865206501068887 28.99448620460454 21.542361398042903 100-101 20.53798962682756 28.834634898719138 29.05290266617735 21.574472808275953 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 295.0 1 215.0 2 274.5 3 395.5 4 298.5 5 234.0 6 381.0 7 776.0 8 1274.5 9 1561.0 10 1410.5 11 1153.5 12 1185.5 13 1350.0 14 1536.0 15 1769.0 16 1971.0 17 2190.5 18 2472.0 19 2758.5 20 3108.5 21 3645.5 22 4444.0 23 5506.0 24 6989.0 25 9143.5 26 11822.0 27 14801.0 28 17869.5 29 21743.5 30 26117.5 31 30513.5 32 35714.5 33 39899.0 34 42998.5 35 45689.5 36 49533.0 37 54368.0 38 57251.0 39 58593.5 40 59714.5 41 60824.5 42 61580.0 43 62507.0 44 63226.5 45 63965.5 46 64141.5 47 63364.5 48 61909.0 49 59561.0 50 56053.0 51 51690.0 52 47658.0 53 43546.5 54 38848.5 55 33114.0 56 27016.0 57 21384.0 58 16657.5 59 12621.0 60 9001.5 61 6335.5 62 4489.0 63 2827.5 64 1511.5 65 871.5 66 492.0 67 268.5 68 141.5 69 78.5 70 40.5 71 23.0 72 15.0 73 10.5 74 9.5 75 8.5 76 7.5 77 4.5 78 3.0 79 3.0 80 1.5 81 0.5 82 0.5 83 0.5 84 1.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005774279562386599 2 0.0058384382241908955 3 0.0022455531631503443 4 0.1727792762389679 5 0.001988918515933162 6 1.9247598541288663E-4 7 0.0 8 5.774279562386599E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.170313017279211E-4 24-25 6.095072871408078E-4 26-27 0.0029192191120954477 28-29 1.9247598541288663E-4 30-31 0.006864976813059624 32-33 0.021396913711732565 34-35 0.01324876366258703 36-37 7.378246107493989E-4 38-39 2.8871397811932995E-4 40-41 0.0015077285524009454 42-43 0.010489941205002323 44-45 0.006832897482157476 46-47 0.009784195925155071 48-49 0.009880433917861515 50-51 0.1849694219817841 52-53 0.11513271860780837 54-55 0.01674541073092114 56-57 0.005678041569680156 58-59 0.01700204537813832 60-61 0.12167690211184652 62-63 0.23664922406514416 64-65 0.2432575662309866 66-67 0.2541003800759125 68-69 0.24951303575690542 70-71 0.2360397167780033 72-73 0.1609420031360754 74-75 0.26048416692543996 76-77 0.20412078253036628 78-79 0.0058384382241908955 80-81 3.207933090214778E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.623799270644331E-5 90-91 0.006159231533212372 92-93 0.018156901290615642 94-95 0.0029192191120954477 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1558636.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.40259642816308 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13039475833264 55.49529650711785 2 12.248424207367343 16.75648035874983 3 3.0766465613879945 6.3135183927215595 4 1.232125395969424 3.371223048375486 5 0.65972208617297 2.2563351807617744 6 0.3995088759707903 1.6396466647499417 7 0.25220225298771876 1.2075902250574686 8 0.18329058042582638 1.0030041281561255 9 0.13026773767388455 0.8019586338944885 >10 0.6504302262977906 7.540428482722509 >50 0.024295806180639437 1.1332364856659565 >100 0.011845500404910185 1.4757700078068452 >500 4.700060155632769E-4 0.18473178433075108 >1k 3.7600481245062147E-4 0.8207800998893275 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4768 0.3059084994828812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4408 0.28281138123333477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 0.16501607816064814 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.415866180429555E-5 0.0 4 0.0 0.0 0.0 6.415866180429555E-5 0.0 5 0.0 0.0 0.0 6.415866180429555E-5 0.0 6 0.0 0.0 0.0 6.415866180429555E-5 0.0 7 0.0 0.0 0.0 6.415866180429555E-5 0.0 8 0.0 0.0 0.0 6.415866180429555E-5 0.0 9 0.0 0.0 0.0 6.415866180429555E-5 0.0 10-11 0.0 0.0 0.0 6.415866180429555E-5 0.0 12-13 0.0 0.0 0.0 6.415866180429555E-5 0.0 14-15 0.0 0.0 0.0 6.415866180429555E-5 0.0 16-17 0.0 0.0 0.0 6.415866180429555E-5 0.0 18-19 0.0 0.0 0.0 6.415866180429555E-5 0.0 20-21 0.0 0.0 0.0 6.415866180429555E-5 0.0 22-23 0.0 0.0 0.0 9.623799270644333E-5 3.2079330902147774E-5 24-25 0.0 0.0 0.0 1.283173236085911E-4 6.415866180429555E-5 26-27 0.0 0.0 0.0 2.8871397811933E-4 6.415866180429555E-5 28-29 0.0 0.0 0.0 9.944592579665809E-4 6.415866180429555E-5 30-31 0.0 0.0 0.0 0.0022134738322481962 6.415866180429555E-5 32-33 0.0 0.0 0.0 0.007153690791178953 6.415866180429555E-5 34-35 0.0 0.0 0.0 0.013601636302510656 6.415866180429555E-5 36-37 0.0 3.2079330902147774E-5 0.0 0.024284053492925867 6.415866180429555E-5 38-39 0.0 6.415866180429555E-5 0.0 0.04208808214361788 6.415866180429555E-5 40-41 0.0 6.415866180429555E-5 0.0 0.062105584626558095 6.415866180429555E-5 42-43 0.0 6.415866180429555E-5 0.0 0.0828609117202477 6.415866180429555E-5 44-45 0.0 6.415866180429555E-5 0.0 0.11022458097977975 6.415866180429555E-5 46-47 0.0 6.415866180429555E-5 0.0 0.1407961833295266 6.415866180429555E-5 48-49 0.0 6.415866180429555E-5 0.0 0.17326046620250013 6.415866180429555E-5 50-51 0.0 6.415866180429555E-5 0.0 0.20835525420944978 6.415866180429555E-5 52-53 0.0 6.415866180429555E-5 0.0 0.2460805473503756 6.415866180429555E-5 54-55 0.0 6.415866180429555E-5 0.0 0.28608347298535386 6.415866180429555E-5 56-57 0.0 6.415866180429555E-5 0.0 0.3358385152145851 6.415866180429555E-5 58-59 0.0 6.415866180429555E-5 0.0 0.39284348622770165 6.415866180429555E-5 60-61 0.0 6.415866180429555E-5 0.0 0.4553340228250855 9.623799270644333E-5 62-63 0.0 6.415866180429555E-5 0.0 0.51663762417909 1.9247598541288665E-4 64-65 0.0 6.415866180429555E-5 0.0 0.580475492674364 1.9247598541288665E-4 66-67 0.0 6.415866180429555E-5 0.0 0.6567280622287692 1.9247598541288665E-4 68-69 0.0 6.415866180429555E-5 0.0 0.7430214623555468 1.9247598541288665E-4 70-71 0.0 6.415866180429555E-5 0.0 0.8380404404877084 1.9247598541288665E-4 72-73 0.0 6.415866180429555E-5 0.0 0.9381921115642139 1.9247598541288665E-4 74-75 0.0 6.415866180429555E-5 0.0 1.0421612230180748 1.9247598541288665E-4 76-77 0.0 6.415866180429555E-5 0.0 1.1606943507015108 1.9247598541288665E-4 78-79 0.0 6.415866180429555E-5 0.0 1.2952671438360208 1.9247598541288665E-4 80-81 0.0 6.415866180429555E-5 0.0 1.4422225586987598 1.9247598541288665E-4 82-83 0.0 6.415866180429555E-5 0.0 1.6011114846570975 1.9247598541288665E-4 84-85 0.0 6.415866180429555E-5 0.0 1.762951709058433 1.9247598541288665E-4 86-87 0.0 6.415866180429555E-5 0.0 1.9475682584002936 1.9247598541288665E-4 88-89 0.0 6.415866180429555E-5 0.0 2.1449203021103065 3.207933090214778E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2220 0.0 59.537094 1 GTATCAA 4335 0.0 42.33446 1 TCAACGC 5585 0.0 32.428574 4 ATCAACG 5600 0.0 32.426594 3 CAACGCA 5625 0.0 32.135994 5 ATCACGC 180 5.456968E-12 31.690914 3 TATCAAC 5780 0.0 31.418789 2 AACGCAG 5925 0.0 30.829157 6 TAACGCA 190 1.2732926E-11 30.02297 4 GTGGTAT 1100 0.0 27.66197 1 TCACGCA 210 4.1836756E-11 27.16364 4 CGCAGAG 6790 0.0 26.761982 8 ACGCAGA 6820 0.0 26.64426 7 TATCACG 205 9.476935E-10 25.508959 2 TGGTATC 1140 0.0 25.437763 2 ATAACGC 210 1.2551027E-9 24.900002 3 GCAGAGT 7705 0.0 23.214428 9 GGCGTCG 105 0.0022114438 22.592796 8 AGAGTAC 7220 0.0 21.7839 10-11 CAGAGTA 7485 0.0 20.061857 10-11 >>END_MODULE