FastQCFastQC Report
Sat 18 Jun 2016
SRR1930977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5927017
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2750414.640462478848972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74650.050.5321431
GGTATCA35950.049.5302771
ATCAACG90000.040.909213
TCAACGC94500.039.5457534
CAACGCA93650.039.4074675
TATCAAC96500.038.2844162
AACGCAG104150.036.0760966
ACGCAGA117200.031.322477
CGCAGAG120750.030.298168
GTACATG95800.026.1074431
GCAGAGT132500.025.7800889
TACATGG96250.025.6070542
GTCCTAC55700.025.5004271
ACATGGG101050.023.4229583
GTGGTAT18350.022.4361061
TCCTACA65750.022.1470322
CCTACAG62300.021.8371753
GAGTACT79350.021.43305212-13
AGAGTAC117950.020.4047910-11
CAGAGTA123000.020.25392210-11
Produced by FastQC (version 0.11.5)