FastQCFastQC Report
Sat 18 Jun 2016
SRR1930977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5927017
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114010.1923564585692938No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA99510.16789221289562692No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC81420.13737095743103148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73710.12436272749006795No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA70850.11953736592960675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61500.051.4713861
GTATCAA127650.047.8091431
ATCAACG160200.037.527593
CAACGCA163700.036.569835
TCAACGC166400.036.2712144
TATCAAC167500.036.0357552
AACGCAG178300.034.0007176
ACGCAGA199850.029.8122067
CGCAGAG201850.029.4463128
GCAGAGT231400.025.0092959
GTACATG154550.024.4799671
TACATGG151600.024.2966982
ACATGGG153400.023.2358513
GTCCTAC87650.023.154891
GTGGTAT30750.022.4124661
AGAGTAC211750.021.39365210-11
GTAGGAC123000.020.3623643
GAGTACT154100.020.02394312-13
GACGTGG101250.019.817737
TCCTACA104800.019.6368272
Produced by FastQC (version 0.11.5)