##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930977_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5927017 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.345686033969532 31.0 31.0 34.0 30.0 34.0 2 31.525865709512896 31.0 31.0 34.0 30.0 34.0 3 31.606208823089254 31.0 31.0 34.0 30.0 34.0 4 35.3670951171559 37.0 35.0 37.0 33.0 37.0 5 35.226657186912064 37.0 35.0 37.0 33.0 37.0 6 35.248637214976775 37.0 35.0 37.0 32.0 37.0 7 35.126179155551604 37.0 35.0 37.0 32.0 37.0 8 35.12241402378296 37.0 35.0 37.0 32.0 37.0 9 36.626494744320794 39.0 35.0 39.0 32.0 39.0 10-11 36.56357219829131 38.0 35.0 39.0 32.0 39.0 12-13 36.456963595683966 38.0 35.0 39.0 32.0 39.0 14-15 37.53377845887738 39.0 36.0 41.0 32.0 41.0 16-17 37.50546632479711 39.0 36.0 41.0 32.0 41.0 18-19 37.52732276624143 39.0 36.0 41.0 32.0 41.0 20-21 37.51147761175647 39.0 36.0 41.0 32.0 41.0 22-23 37.40765683648284 39.0 36.0 41.0 32.0 41.0 24-25 37.3044351821498 39.0 36.0 41.0 31.5 41.0 26-27 37.07149034666173 39.0 36.0 40.0 31.0 41.0 28-29 36.891295233335754 39.0 36.0 40.0 30.5 41.0 30-31 36.66623092189545 38.5 35.5 40.0 30.0 41.0 32-33 36.532383912514504 38.0 35.0 40.0 30.0 41.0 34-35 36.623140190082125 38.5 35.0 40.0 30.0 41.0 36-37 36.67880571289065 39.0 35.0 40.0 30.0 41.0 38-39 36.65220160495575 39.0 35.0 40.0 30.0 41.0 40-41 36.52412292726679 39.0 35.0 40.0 30.0 41.0 42-43 36.4201614404008 39.0 35.0 40.0 30.0 41.0 44-45 36.281741726065576 39.0 35.0 40.0 30.0 41.0 46-47 36.066047220718275 38.5 35.0 40.0 29.5 41.0 48-49 35.880912354393445 38.0 35.0 40.0 29.0 41.0 50-51 35.640513178889144 38.0 34.5 40.0 27.5 41.0 52-53 35.44575871808702 38.0 34.0 40.0 27.0 41.0 54-55 35.31460716242252 38.0 34.0 40.0 27.0 41.0 56-57 35.136119400366155 38.0 34.0 40.0 27.0 41.0 58-59 34.932926293277035 38.0 34.0 40.0 26.5 41.0 60-61 34.636409850013926 38.0 33.0 40.0 26.0 41.0 62-63 34.38859210290775 37.0 33.0 40.0 26.0 41.0 64-65 34.07135039430459 37.0 33.0 39.5 26.0 41.0 66-67 33.73963867490173 36.0 32.0 39.0 25.0 41.0 68-69 33.382970725408754 36.0 32.0 39.0 24.5 40.0 70-71 33.002063263864436 35.5 32.0 39.0 23.5 40.0 72-73 32.58773730529202 35.0 31.0 38.0 22.5 40.0 74-75 32.088313058660034 35.0 31.0 37.0 22.0 39.0 76-77 30.66037755585989 33.5 29.5 36.0 20.5 38.5 78-79 31.432392129126676 34.0 30.5 36.0 22.0 39.0 80-81 31.412187192984263 34.0 31.0 36.0 22.0 38.5 82-83 31.165299171573153 34.0 31.0 35.5 21.5 37.0 84-85 30.849227866226805 34.0 31.0 35.0 20.5 37.0 86-87 30.526917418998462 34.0 30.0 35.0 20.0 36.0 88-89 30.202823106463168 34.0 30.0 35.0 18.5 36.0 90-91 29.88682468769703 34.0 30.0 35.0 17.5 36.0 92-93 29.6071944791115 34.0 30.0 35.0 13.0 35.0 94-95 29.26403855430143 34.0 29.0 35.0 4.5 35.0 96-97 28.84003158755914 34.0 29.0 35.0 2.0 35.0 98-99 28.379816609265674 34.0 29.0 35.0 2.0 35.0 100-101 27.31156600360687 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39246775301282355 1101 2 0.4337559916401581 1101 3 0.36279397755723863 1101 4 0.2573618820598895 1101 5 0.30752474653399275 1101 6 0.3318613995646871 1101 7 0.4048944249169395 1101 8 0.41505511372771053 1101 9 0.5529608441468454 1101 10-11 0.49161091620604935 1101 12-13 0.43177978492508373 1101 14-15 0.4712946132832272 1101 16-17 0.6200499635454833 1101 18-19 0.5796834421140673 1101 20-21 0.5480284284275356 1101 22-23 0.5999034318965357 1101 24-25 0.5527105740488523 1101 26-27 0.4930393425797135 1101 28-29 0.5767376256325818 1101 30-31 0.6713851053813116 1101 32-33 0.5560522315773113 1101 34-35 0.5459882811014865 1101 36-37 0.58714888157769 1101 38-39 0.597981519863886 1101 40-41 0.6504459534020057 1101 42-43 0.7043245932858966 1101 44-45 0.6410428865491653 1101 46-47 0.6872289460889505 1101 48-49 0.7113739965443884 1101 50-51 0.7523446612909197 1101 52-53 0.5433262565766768 1101 54-55 0.5771010183326695 1101 56-57 0.6324283923654335 1101 58-59 0.5922402423542295 1101 60-61 0.5632957591691721 1101 62-63 0.37449020614751305 1101 64-65 0.5475429286943836 1101 66-67 0.5421292144046319 1101 68-69 0.47553843654797134 1101 70-71 0.49009845567952226 1101 72-73 0.4887374220513081 1101 74-75 0.5360172118740394 1101 76-77 0.5333834015165699 1101 78-79 0.5677813564662664 1101 80-81 0.4742437120985592 1101 82-83 0.4723068140371396 1101 84-85 0.572490004053062 1101 86-87 0.5270763553024977 1101 88-89 0.47934404157622623 1101 90-91 0.5663911472320926 1101 92-93 0.37142234516215566 1101 94-95 0.5486900950523683 1101 96-97 0.6146420105134389 1101 98-99 0.8098977003590093 1101 100-101 0.9736074674604964 1102 1 0.3413372710662017 1102 2 0.44773571593339057 1102 3 0.4087656304511569 1102 4 0.21549038361600026 1102 5 0.2963245035747164 1102 6 0.3664588868184566 1102 7 0.4251167598954879 1102 8 0.4373129756179992 1102 9 0.49102635213387913 1102 10-11 0.4179400486265976 1102 12-13 0.5160083133837929 1102 14-15 0.5857669275972341 1102 16-17 0.6087922932859158 1102 18-19 0.6109405489359645 1102 20-21 0.5561951787327146 1102 22-23 0.5470861197480446 1102 24-25 0.5709794056370256 1102 26-27 0.5918975764007683 1102 28-29 0.5848173526810854 1102 30-31 0.5478416149592888 1102 32-33 0.5855165385752983 1102 34-35 0.6031784731996694 1102 36-37 0.5977918939754829 1102 38-39 0.6990859196000017 1102 40-41 0.663461038361902 1102 42-43 0.6781956669844718 1102 44-45 0.7583785167681185 1102 46-47 0.6603964966613489 1102 48-49 0.6309384870930259 1102 50-51 0.5424139486444375 1102 52-53 0.5921053614599785 1102 54-55 0.6542289629523239 1102 56-57 0.5884912602445596 1102 58-59 0.6577113582796699 1102 60-61 0.5520514318564693 1102 62-63 0.34846762096549355 1102 64-65 0.4796817845010821 1102 66-67 0.42871938254123876 1102 68-69 0.5325571980934143 1102 70-71 0.5990368679183788 1102 72-73 0.5527516955990635 1102 74-75 0.6957933816186141 1102 76-77 0.6974028295901924 1102 78-79 0.7138164734925816 1102 80-81 0.7788485457126306 1102 82-83 0.7340597212361679 1102 84-85 0.7883754324096408 1102 86-87 0.6778044746497436 1102 88-89 0.7097950920004905 1102 90-91 0.6987023313768326 1102 92-93 0.7655283228333047 1102 94-95 0.8975656344587968 1102 96-97 1.0905121424662063 1102 98-99 1.1096295257020046 1102 100-101 1.3065234183497871 1103 1 0.3865748288201267 1103 2 0.5063872586454572 1103 3 0.38989282625523813 1103 4 0.3008099409550411 1103 5 0.3433998072070281 1103 6 0.3709442947231949 1103 7 0.42076586802925675 1103 8 0.3964185399591287 1103 9 0.4662107829523947 1103 10-11 0.5051237810656701 1103 12-13 0.5375724433201583 1103 14-15 0.5475861970197542 1103 16-17 0.6446959259239335 1103 18-19 0.6521304758926121 1103 20-21 0.6450259376668583 1103 22-23 0.6029226653617812 1103 24-25 0.6602233874017642 1103 26-27 0.5964825663728135 1103 28-29 0.5429729799978631 1103 30-31 0.526124324533491 1103 32-33 0.7128488386390615 1103 34-35 0.6532220544162044 1103 36-37 0.6337879838906417 1103 38-39 0.5771134116903553 1103 40-41 0.6077430642562405 1103 42-43 0.6455667079073208 1103 44-45 0.6376054935336484 1103 46-47 0.630489132433226 1103 48-49 0.5330663844264549 1103 50-51 0.5220511933275844 1103 52-53 0.5368567685310452 1103 54-55 0.6242522557663435 1103 56-57 0.5748233777636997 1103 58-59 0.6219072327111661 1103 60-61 0.5959329095469457 1103 62-63 0.5991814424434452 1103 64-65 0.5033765566900428 1103 66-67 0.4770043405492288 1103 68-69 0.4884573268346202 1103 70-71 0.4882291912044536 1103 72-73 0.44290822048940726 1103 74-75 0.5151640811515108 1103 76-77 0.554912965916678 1103 78-79 0.6208625309861837 1103 80-81 0.5619872422780183 1103 82-83 0.5790171322404163 1103 84-85 0.41987396477786376 1103 86-87 0.34749000741080494 1103 88-89 0.48443821876040616 1103 90-91 0.5526704570155339 1103 92-93 0.37960069840184474 1103 94-95 0.4508951839035653 1103 96-97 0.7122538521728536 1103 98-99 0.6420565607258197 1103 100-101 0.9424060516062376 1104 1 0.3410395136429223 1104 2 0.3733948271293954 1104 3 0.40253923377989054 1104 4 0.21406869427487152 1104 5 0.31964174630386566 1104 6 0.2981732335039453 1104 7 0.33799722055500325 1104 8 0.3354536338212526 1104 9 0.4688898299576678 1104 10-11 0.42169898204883793 1104 12-13 0.447871921691835 1104 14-15 0.5443804572244488 1104 16-17 0.49887420824110507 1104 18-19 0.6150654126528181 1104 20-21 0.5056743536067998 1104 22-23 0.5535802447899698 1104 24-25 0.49655680953338077 1104 26-27 0.6382426842940205 1104 28-29 0.6493672386840856 1104 30-31 0.6285831838130775 1104 32-33 0.5977439888406906 1104 34-35 0.6131884126835416 1104 36-37 0.5681902288431644 1104 38-39 0.6646846869802872 1104 40-41 0.5314374469255014 1104 42-43 0.6579382654268215 1104 44-45 0.7650069404519897 1104 46-47 0.49732782792038677 1104 48-49 0.5770086796721827 1104 50-51 0.4599677192787368 1104 52-53 0.49138777166626113 1104 54-55 0.4955759787728695 1104 56-57 0.6308530194610498 1104 58-59 0.6492667144597277 1104 60-61 0.5451865164967131 1104 62-63 0.4425201749003378 1104 64-65 0.4917980580532344 1104 66-67 0.5135336950445648 1104 68-69 0.3693862479544592 1104 70-71 0.5064516286336627 1104 72-73 0.47902325808622237 1104 74-75 0.5270121237532877 1104 76-77 0.4789864931964658 1104 78-79 0.5992443860198229 1104 80-81 0.578498992223583 1104 82-83 0.5243473035380859 1104 84-85 0.5615460570743203 1104 86-87 0.5872195914721843 1104 88-89 0.6733841955927709 1104 90-91 0.5961668867119521 1104 92-93 0.573613339661506 1104 94-95 0.7796628110651191 1104 96-97 0.8149816083393411 1104 98-99 0.8933108784261421 1104 100-101 1.0544791968511333 1105 1 0.25428800222254466 1105 2 0.2806248457570497 1105 3 0.31484833067166207 1105 4 0.09056219072503069 1105 5 0.12531451636519364 1105 6 0.20882101896654603 1105 7 0.2488821629247937 1105 8 0.25676225912864226 1105 9 0.31540963781149856 1105 10-11 0.36387538879636594 1105 12-13 0.3925817608224236 1105 14-15 0.4873159480368301 1105 16-17 0.47450203511104405 1105 18-19 0.4853260274714799 1105 20-21 0.4155772673256095 1105 22-23 0.4440789566519072 1105 24-25 0.47677473362745104 1105 26-27 0.39265982818543677 1105 28-29 0.39044717382297023 1105 30-31 0.3606952132953083 1105 32-33 0.47577349119303136 1105 34-35 0.4828539158514076 1105 36-37 0.47359625941633254 1105 38-39 0.51542112920486 1105 40-41 0.47587743116099546 1105 42-43 0.47265787397731884 1105 44-45 0.5355088063188234 1105 46-47 0.5818098618785967 1105 48-49 0.5621341554437649 1105 50-51 0.39439402742915775 1105 52-53 0.3216474711618318 1105 54-55 0.43755454339461153 1105 56-57 0.4910950093845017 1105 58-59 0.5863433241084763 1105 60-61 0.4255504925224187 1105 62-63 0.3175824037754964 1105 64-65 0.275713143087998 1105 66-67 0.33698264627590646 1105 68-69 0.24992060641289982 1105 70-71 0.2527679976571946 1105 72-73 0.3513000236707313 1105 74-75 0.47070435518281073 1105 76-77 0.4503261967558494 1105 78-79 0.5961402414119981 1105 80-81 0.5099407543087757 1105 82-83 0.5127671429457479 1105 84-85 0.5646174347692998 1105 86-87 0.5847290166120587 1105 88-89 0.6235097285720421 1105 90-91 0.5882969690124575 1105 92-93 0.6737754097474102 1105 94-95 0.623904374205388 1105 96-97 0.675520471731371 1105 98-99 0.7240363863994475 1105 100-101 0.8040484367014891 1106 1 0.28283505804521525 1106 2 0.2223577455241852 1106 3 0.30475224651546995 1106 4 0.1152334455905688 1106 5 0.23353699551421414 1106 6 0.20460250459370144 1106 7 0.28860141448653565 1106 8 0.24077974237137312 1106 9 0.36136049135156867 1106 10-11 0.2793307681129562 1106 12-13 0.35865034301451715 1106 14-15 0.40356382876893093 1106 16-17 0.3873275868281212 1106 18-19 0.39107995827796316 1106 20-21 0.37071966370871934 1106 22-23 0.3659084771494463 1106 24-25 0.4121398990698282 1106 26-27 0.4291287347780113 1106 28-29 0.4582786692339269 1106 30-31 0.5470548295993609 1106 32-33 0.5273606029475602 1106 34-35 0.5905898076203826 1106 36-37 0.4652022900832833 1106 38-39 0.4918470351143043 1106 40-41 0.36731990012845017 1106 42-43 0.40705537722546126 1106 44-45 0.5354286805735953 1106 46-47 0.4565786611296403 1106 48-49 0.5229223001970666 1106 50-51 0.40301788277088235 1106 52-53 0.29165127790221845 1106 54-55 0.4447090797023989 1106 56-57 0.5807268207068503 1106 58-59 0.6142687680568883 1106 60-61 0.5844115483669867 1106 62-63 0.4283706553097346 1106 64-65 0.4189527752597044 1106 66-67 0.3694795956780226 1106 68-69 0.379918841857922 1106 70-71 0.4357633337328437 1106 72-73 0.5027302341866786 1106 74-75 0.6839359013681019 1106 76-77 0.644276949921835 1106 78-79 0.5656002662332291 1106 80-81 0.4635578384043697 1106 82-83 0.5851193160846293 1106 84-85 0.5386985514416232 1106 86-87 0.5437387248678505 1106 88-89 0.5270118362942533 1106 90-91 0.6054874317663312 1106 92-93 0.707734136779834 1106 94-95 0.7905248937106606 1106 96-97 0.7020693625844352 1106 98-99 0.6870933457535529 1106 100-101 0.9808713407852458 1107 1 0.19982369772747433 1107 2 0.24490686022876673 1107 3 0.19357090697422308 1107 4 0.11949721523019718 1107 5 0.21060431885146613 1107 6 0.22417605461998136 1107 7 0.24957222518684574 1107 8 0.2230011371497156 1107 9 0.30542667573665483 1107 10-11 0.1895929930747826 1107 12-13 0.23763372912346625 1107 14-15 0.28249136241599615 1107 16-17 0.3375605661684489 1107 18-19 0.368644373741013 1107 20-21 0.2902402555883654 1107 22-23 0.2832626349506384 1107 24-25 0.2654762623079634 1107 26-27 0.37392238313800163 1107 28-29 0.3873846856548582 1107 30-31 0.4591369530567704 1107 32-33 0.43028545192166234 1107 34-35 0.41350686541073145 1107 36-37 0.4328787077241145 1107 38-39 0.49419964406571637 1107 40-41 0.39260312582455015 1107 42-43 0.47025567114187083 1107 44-45 0.48429932397726816 1107 46-47 0.47693998640269797 1107 48-49 0.46021758606154606 1107 50-51 0.5567724876003695 1107 52-53 0.5080423066722304 1107 54-55 0.486105662428713 1107 56-57 0.5089107500083756 1107 58-59 0.5524532345016127 1107 60-61 0.5742675209820618 1107 62-63 0.34519416257316493 1107 64-65 0.4009122475452003 1107 66-67 0.3863850042406014 1107 68-69 0.4814802075992404 1107 70-71 0.47000798524685194 1107 72-73 0.45338197661660473 1107 74-75 0.5290975710385979 1107 76-77 0.5083942984996064 1107 78-79 0.6503892054640836 1107 80-81 0.6595556262088174 1107 82-83 0.7527372476974392 1107 84-85 0.7339074949279016 1107 86-87 0.6509264830094779 1107 88-89 0.653617425086626 1107 90-91 0.7247069887848632 1107 92-93 0.6820757452472961 1107 94-95 0.7561479902420736 1107 96-97 0.6993282113666304 1107 98-99 0.800087387114619 1107 100-101 1.006625948743725 1108 1 0.12847544215791018 1108 2 0.11484433395722604 1108 3 0.0902712012155007 1108 4 0.04562163725625368 1108 5 0.11219637433696761 1108 6 0.1407350079617231 1108 7 0.12999729874716337 1108 8 0.12673695051667977 1108 9 0.11296135417297393 1108 10-11 0.14050330202812233 1108 12-13 0.12332756056524374 1108 14-15 0.17227567305072 1108 16-17 0.2372676384677348 1108 18-19 0.20851406688939988 1108 20-21 0.0942716238103074 1108 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-1.0981261646569642 2316 90-91 -1.2403250624971882 2316 92-93 -1.2800973316757833 2316 94-95 -1.6868071379630543 2316 96-97 -1.443370506582088 2316 98-99 -1.6743453051930715 2316 100-101 -1.977181743563964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 19.0 4 50.0 5 109.0 6 250.0 7 698.0 8 1841.0 9 3653.0 10 6266.0 11 10176.0 12 14604.0 13 18808.0 14 22682.0 15 28097.0 16 32914.0 17 37274.0 18 31656.0 19 25690.0 20 25011.0 21 26721.0 22 29841.0 23 35708.0 24 44100.0 25 57664.0 26 76039.0 27 98554.0 28 127526.0 29 161234.0 30 199044.0 31 240399.0 32 291642.0 33 355870.0 34 440929.0 35 553047.0 36 732396.0 37 1028525.0 38 994587.0 39 173361.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.42465030440078 21.820613944359838 14.981306711327639 23.773429039911747 2 14.407434279350722 21.93314885431782 41.364391050518 22.295025815813453 3 18.179051131048872 26.556719141647356 30.735370737560757 24.528858989743014 4 12.632612422038179 18.228303111982772 39.24413327884329 29.894951187135753 5 12.323451218958704 38.70243869353016 35.9463618689221 13.027748218589034 6 29.280428547325798 39.25836004724144 18.282048253754006 13.179163151678758 7 25.954253210341726 33.193324736541165 23.41785083457665 17.434571218540455 8 23.73179532716572 37.76927916590251 21.1803614690177 17.318564037914076 9 25.020410773243945 17.126203619797277 22.00025746509585 35.853128141862925 10-11 23.60055657002502 27.04843768796344 29.924285690424036 19.426720051587502 12-13 25.04711898076216 24.7483514894592 29.438012409952595 20.766517119826045 14-15 20.958013111823366 28.061923898649187 26.796211315067936 24.183851674459515 16-17 20.33107041872834 30.128064420938898 28.84169220368357 20.699172956649186 18-19 20.86762194203256 28.25627124065951 30.808178886613618 20.06792793069431 20-21 21.50386104848358 28.205520584806827 29.858890230954287 20.431728135755307 22-23 20.94779997872448 28.81673096142492 29.861922546138224 20.373546513712377 24-25 21.147203612633927 28.15867784212105 30.470766929687183 20.223351615557846 26-27 20.309362799188776 29.06075054919468 30.10096408553602 20.52892256608052 28-29 20.607196853319053 28.69578186764416 29.714339800847206 20.982681478189583 30-31 21.068985910240954 28.019005693777842 30.106493640162352 20.805514755818848 32-33 20.558938840566626 28.85163267467208 30.054073027486243 20.535355457275053 34-35 20.86717620420492 28.700900328851926 30.189394645679496 20.242528821263658 36-37 20.697435797068938 28.503433078815927 29.979668358284677 20.81946276583046 38-39 21.098569444671746 28.709233796057664 29.681428999734944 20.510767759535646 40-41 21.826368047058537 27.77905069010595 30.059688715007944 20.33489254782757 42-43 20.845228299401413 28.783122297082787 29.92767135057716 20.44397805293864 44-45 20.541723072074003 29.071325850333135 29.37955542387029 21.007395653722572 46-47 21.377788280706152 28.04656190754928 29.32364637862564 21.252003433118933 48-49 21.028930779193352 28.454672643421485 29.14494783472846 21.3714487426567 50-51 20.784600303364854 29.103949541292934 28.986904057357023 21.124546097985192 52-53 20.970404002235774 28.82339139719305 28.931852567806814 21.274352032764362 54-55 20.881224017026 28.469847094058114 29.350163246819605 21.29876564209628 56-57 21.144477913891237 28.525465346467833 28.458791733754367 21.871265005886563 58-59 21.124720604788802 28.709454208294527 28.422631942334498 21.743193244582173 60-61 20.77211762369651 28.309909632313396 29.503980757959425 21.413991986030666 62-63 21.174332278154793 28.251802870334814 29.261403492600657 21.312461358909733 64-65 21.045186714906738 28.546976858415064 28.775452195704993 21.63238423097321 66-67 21.089126103048237 29.032046071222833 28.282887146428916 21.595940679300014 68-69 21.417926498014058 28.106362121169965 29.21665840231173 21.259052978504247 70-71 21.79087378100555 27.7205813271965 28.92300765900721 21.565537232790742 72-73 21.385878175754293 28.717251717212005 28.316756220536583 21.580113886497124 74-75 20.76748694801877 29.26231352499924 28.786740946509713 21.183458580472273 76-77 20.96576690024023 28.388736360234123 29.178601563076572 21.46689517644908 78-79 20.85461491853535 28.432625275428187 28.881173122022258 21.831586684014205 80-81 21.134388799894314 28.640999842163396 28.646457901113976 21.578153456828314 82-83 20.559321830863652 28.80200107406474 29.22311510157639 21.41556199349521 84-85 20.861219058423487 28.252820938424843 29.136984084911514 21.748975918240156 86-87 20.165692117974356 29.142315603279016 28.4522214125588 22.23977086618783 88-89 20.697518353470656 28.40641891678143 28.729574975588463 22.16648775415945 90-91 20.602598807646004 28.29475594217639 29.309151945874586 21.793493304303023 92-93 20.424376886026916 28.876840253915702 29.001638124986023 21.697144735071358 94-95 20.18488896597681 28.791216318875385 28.903957402699866 22.11993731244794 96-97 20.629164774553942 28.42844287678295 28.659705941429387 22.282686407233722 98-99 20.635818996301175 28.308202929061956 28.97974647280411 22.07623160183276 100-101 20.394407507182787 28.384733838286614 28.85540061720761 22.36545803732299 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 271.0 1 206.0 2 179.5 3 250.5 4 245.0 5 257.0 6 440.5 7 924.0 8 1579.5 9 1792.5 10 1575.5 11 1519.0 12 1767.0 13 2058.5 14 2406.5 15 2902.5 16 3356.0 17 3867.0 18 4570.5 19 5356.5 20 6211.0 21 7260.5 22 8522.5 23 10582.0 24 13621.5 25 17349.0 26 23514.5 27 32264.0 28 42263.0 29 57284.5 30 74736.5 31 94420.5 32 122108.5 33 152994.0 34 188554.0 35 227893.5 36 270178.0 37 292786.5 38 279287.5 39 255562.0 40 240783.5 41 232056.0 42 223485.0 43 224108.0 44 230409.5 45 234886.0 46 231386.0 47 224533.5 48 223757.5 49 221744.5 50 212553.5 51 197463.5 52 179362.5 53 162119.0 54 145598.5 55 125407.0 56 102987.0 57 81447.5 58 64184.5 59 49992.0 60 36464.0 61 25234.0 62 17240.0 63 11133.0 64 6679.5 65 3908.5 66 2223.5 67 1243.5 68 695.0 69 402.5 70 243.0 71 152.0 72 105.0 73 77.5 74 50.0 75 40.0 76 25.0 77 10.5 78 10.5 79 10.5 80 7.5 81 7.5 82 3.5 83 1.5 84 1.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00490972102830142 2 0.007035579617875231 3 0.002176474270277949 4 0.1744385076000288 5 0.0019233958667572576 6 2.8682219065678403E-4 7 0.0 8 6.073881684496602E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.9525813744080706E-4 24-25 6.664397959378217E-4 26-27 0.0026488872901832402 28-29 1.687189356804612E-4 30-31 0.006276344407313156 32-33 0.020828352609752934 34-35 0.013387847546244596 36-37 8.182868380502367E-4 38-39 3.036940842248301E-4 40-41 0.0017040612503726581 42-43 0.011228245169534691 44-45 0.007221170447123738 46-47 0.010587113213948939 48-49 0.010334034810428248 50-51 0.19179968608154827 52-53 0.11685473485228742 54-55 0.017226203332975087 56-57 0.006487243076913732 58-59 0.01767330851252831 60-61 0.1261764560486329 62-63 0.24348673202725757 64-65 0.2493918947760737 66-67 0.25961626227830964 68-69 0.25445346284648757 70-71 0.24136087343768373 72-73 0.16402854926854438 74-75 0.2705745571507556 76-77 0.21028284548534282 78-79 0.005668956238863495 80-81 2.5307840352069175E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.592352105620752E-5 90-91 0.005694264079215565 92-93 0.01745397389614371 94-95 0.002741682704807494 96-97 8.43594678402306E-6 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5927017.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.5595343404558 #Duplication Level Percentage of deduplicated Percentage of total 1 66.61867828550972 29.01878604493437 2 16.96244205867697 14.777521547058626 3 6.537855032776008 8.543577624393848 4 3.2763993709173227 5.708737236420835 5 1.8017565664308135 3.9241838514292375 6 1.1334096895226753 2.9622478975540996 7 0.7505392297861712 2.288519754761097 8 0.517001529189409 1.8016276691835376 9 0.37181438058735927 1.457645515152334 >10 1.7930310032537342 13.875262662408252 >50 0.1377700986987838 4.164393100621716 >100 0.09107157821057871 7.483741662631112 >500 0.006134566123937703 1.7867111724307043 >1k 0.0018636536148186557 1.3778086623697037 >5k 1.9413058483270414E-4 0.6364164625893745 >10k+ 3.882611696654083E-5 0.19281913606116022 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11401 0.1923564585692938 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 9951 0.16789221289562692 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 8142 0.13737095743103148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7371 0.12436272749006795 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 7085 0.11953736592960675 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.687189356804612E-5 0.0 10-11 0.0 0.0 0.0 1.687189356804612E-5 0.0 12-13 0.0 0.0 0.0 1.687189356804612E-5 0.0 14-15 0.0 0.0 0.0 1.687189356804612E-5 0.0 16-17 0.0 0.0 0.0 1.687189356804612E-5 0.0 18-19 0.0 0.0 0.0 3.374378713609224E-5 0.0 20-21 0.0 0.0 0.0 3.374378713609224E-5 0.0 22-23 0.0 0.0 0.0 6.748757427218448E-5 0.0 24-25 0.0 0.0 0.0 1.6028298889643813E-4 0.0 26-27 0.0 0.0 0.0 3.543097649289685E-4 0.0 28-29 0.0 0.0 0.0 0.001121980922275067 0.0 30-31 0.0 0.0 0.0 0.005086875910765905 0.0 32-33 0.0 0.0 0.0 0.0170490484505106 0.0 34-35 0.0 0.0 0.0 0.035970877087074325 0.0 36-37 0.0 0.0 0.0 0.06419755502641548 0.0 38-39 0.0 0.0 0.0 0.10924551085309861 0.0 40-41 0.0 0.0 0.0 0.15962498504728434 0.0 42-43 0.0 0.0 0.0 0.21831386682373274 0.0 44-45 0.0 0.0 0.0 0.29035685235928965 0.0 46-47 0.0 0.0 0.0 0.3753152724211859 0.0 48-49 0.0 0.0 0.0 0.4642892031522771 0.0 50-51 0.0 0.0 0.0 0.5579450843485012 0.0 52-53 0.0 0.0 0.0 0.6574470766660531 0.0 54-55 0.0 0.0 0.0 0.7745633258686453 0.0 56-57 0.0 0.0 0.0 0.9115462297476117 0.0 58-59 0.0 0.0 0.0 1.0597573787961128 0.0 60-61 0.0 0.0 0.0 1.2170287346906548 0.0 62-63 0.0 0.0 0.0 1.386928702920879 0.0 64-65 0.0 0.0 0.0 1.5728907138278836 0.0 66-67 0.0 0.0 0.0 1.793743800633607 0.0 68-69 0.0 0.0 0.0 2.0423595883055508 0.0 70-71 0.0 0.0 0.0 2.3119133958954396 0.0 72-73 0.0 0.0 0.0 2.5892114026330617 0.0 74-75 0.0 0.0 0.0 2.884064614628236 0.0 76-77 0.0 0.0 0.0 3.233321247433574 0.0 78-79 0.0 0.0 0.0 3.614558554497144 0.0 80-81 0.0 0.0 0.0 4.018446378675817 0.0 82-83 0.0 0.0 0.0 4.4454486970427105 0.0 84-85 0.0 0.0 0.0 4.88753448825944 0.0 86-87 0.0 0.0 0.0 5.385550606654241 0.0 88-89 0.0 0.0 0.0 5.9258392543837815 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6150 0.0 51.471386 1 GTATCAA 12765 0.0 47.809143 1 ATCAACG 16020 0.0 37.52759 3 CAACGCA 16370 0.0 36.56983 5 TCAACGC 16640 0.0 36.271214 4 TATCAAC 16750 0.0 36.035755 2 AACGCAG 17830 0.0 34.000717 6 ACGCAGA 19985 0.0 29.812206 7 CGCAGAG 20185 0.0 29.446312 8 GCAGAGT 23140 0.0 25.009295 9 GTACATG 15455 0.0 24.479967 1 TACATGG 15160 0.0 24.296698 2 ACATGGG 15340 0.0 23.235851 3 GTCCTAC 8765 0.0 23.15489 1 GTGGTAT 3075 0.0 22.412466 1 AGAGTAC 21175 0.0 21.393652 10-11 GTAGGAC 12300 0.0 20.362364 3 GAGTACT 15410 0.0 20.023943 12-13 GACGTGG 10125 0.0 19.81773 7 TCCTACA 10480 0.0 19.636827 2 >>END_MODULE