FastQCFastQC Report
Sat 18 Jun 2016
SRR1930976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983087
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN872684.400613790519528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24890.12551138704454218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.058.3210071
GTATCAA34300.047.0422131
ATCAACG42650.037.5532153
TCAACGC42900.037.3217054
TATCAAC44100.036.231592
CAACGCA43950.035.6147165
AACGCAG48050.033.2000056
ACGCAGA56400.028.6870217
TATCGGC1105.6795037E-527.9337942
CGCAGAG56800.027.7395978
GTACATG40700.025.4230861
TACATGG40200.025.0964072
ACATGGG40000.024.6794283
GCAGAGT63700.024.2593579
AGTACTT45150.021.53432512-13
GAGTACT41700.021.53126312-13
AGAGTAC57150.020.96674210-11
CAGAGTA60500.020.10872810-11
GTGGTAT9100.019.7014391
ACGGTAG1300.005415384719.357745
Produced by FastQC (version 0.11.5)