##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930976_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983087 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.75478887209689 31.0 10.0 31.0 2.0 33.0 2 21.2990559667831 30.0 2.0 31.0 2.0 34.0 3 22.11592784381119 30.0 10.0 31.0 2.0 34.0 4 23.60788255885899 33.0 2.0 37.0 2.0 37.0 5 22.909658023072108 33.0 2.0 37.0 2.0 37.0 6 24.05188930188136 33.0 2.0 37.0 2.0 37.0 7 24.409780307167562 33.0 2.0 37.0 2.0 37.0 8 24.975622854670522 33.0 10.0 37.0 2.0 37.0 9 26.620244598446764 35.0 16.0 39.0 2.0 39.0 10-11 27.177233021042447 35.0 16.0 39.0 2.0 39.0 12-13 27.1702305546857 35.0 12.5 39.0 2.0 39.0 14-15 27.23196158312772 36.0 6.0 39.0 2.0 41.0 16-17 25.93552678223396 34.5 2.0 39.0 2.0 41.0 18-19 24.71954054461554 33.5 2.0 39.0 2.0 40.0 20-21 24.348898459825513 33.5 2.0 39.0 2.0 40.0 22-23 23.978802745416615 32.0 2.0 39.0 2.0 40.0 24-25 23.85524765176717 32.0 2.0 39.0 2.0 40.0 26-27 23.607566637268057 32.0 2.0 38.0 2.0 40.0 28-29 23.487054526604226 32.0 2.0 38.0 2.0 40.0 30-31 23.617322891027978 32.0 2.0 38.0 2.0 40.0 32-33 23.54042485276743 32.0 2.0 38.0 2.0 40.0 34-35 23.26673010311701 31.0 2.0 38.0 2.0 40.0 36-37 22.744783259635106 30.0 2.0 38.0 2.0 40.0 38-39 22.738959511105666 30.0 2.0 38.0 2.0 40.0 40-41 22.711615274569397 30.0 2.0 38.0 2.0 40.0 42-43 22.627936394116848 30.0 2.0 38.0 2.0 40.0 44-45 22.265999171998 30.0 2.0 38.0 2.0 40.0 46-47 21.93321120051717 30.0 2.0 38.0 2.0 40.0 48-49 21.802546988609173 29.5 2.0 38.0 2.0 40.0 50-51 19.03174722036905 17.0 2.0 35.5 2.0 38.5 52-53 21.269286218910214 28.0 2.0 36.5 2.0 39.0 54-55 22.200613740093097 30.0 2.0 38.0 2.0 40.0 56-57 22.248541541546086 30.5 2.0 38.0 2.0 40.0 58-59 21.999441527275405 30.0 2.0 38.0 2.0 40.0 60-61 21.74271325463784 29.0 2.0 38.0 2.0 40.0 62-63 21.32755421219543 28.5 2.0 37.0 2.0 40.0 64-65 21.044492500833297 28.0 2.0 37.0 2.0 39.5 66-67 20.75659338193433 28.0 2.0 36.0 2.0 39.0 68-69 20.343831359894953 26.5 2.0 36.0 2.0 39.0 70-71 20.04266882895203 26.0 2.0 35.0 2.0 39.0 72-73 19.669706119802107 26.0 2.0 35.0 2.0 38.0 74-75 19.26544851537023 25.5 2.0 34.5 2.0 37.5 76-77 18.918573163961035 25.0 2.0 34.0 2.0 37.0 78-79 18.601284008215472 24.0 2.0 34.0 2.0 36.5 80-81 18.231644653008164 22.5 2.0 34.0 2.0 36.0 82-83 17.81796814764052 20.5 2.0 34.0 2.0 35.5 84-85 17.48789185749289 20.0 2.0 33.0 2.0 35.0 86-87 17.0412626879204 18.0 2.0 33.0 2.0 35.0 88-89 16.686337765312363 13.5 2.0 32.5 2.0 35.0 90-91 16.2620996960799 7.0 2.0 32.0 2.0 35.0 92-93 15.755763362878179 2.0 2.0 32.0 2.0 34.5 94-95 15.235526983939685 2.0 2.0 32.0 2.0 34.0 96-97 14.617741178274073 2.0 2.0 31.0 2.0 34.0 98-99 13.930579697209453 2.0 2.0 31.0 2.0 34.0 100-101 12.762014979675627 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.086951885387617 1101 2 9.922060679577132 1101 3 9.143229102059411 1101 4 11.280508494977568 1101 5 11.977572620924576 1101 6 11.134756607849557 1101 7 10.591963680201701 1101 8 9.983287035333692 1101 9 9.81579943986798 1101 10-11 9.138233212245272 1101 12-13 9.002913252845719 1101 14-15 9.991079688155168 1101 16-17 11.294565788675147 1101 18-19 12.521354114961738 1101 20-21 12.611898632503035 1101 22-23 12.920632831164074 1101 24-25 12.843247669589477 1101 26-27 12.859062614006959 1101 28-29 12.81793356859308 1101 30-31 12.257721882510108 1101 32-33 12.2047579468651 1101 34-35 12.2794564415222 1101 36-37 12.890498215423566 1101 38-39 12.565469521799933 1101 40-41 12.146211404958475 1101 42-43 11.975344954821551 1101 44-45 11.977143518176714 1101 46-47 11.93649993038343 1101 48-49 11.837903789000123 1101 50-51 13.894823595818789 1101 52-53 12.451275970819552 1101 54-55 11.967744664472047 1101 56-57 11.946110490885932 1101 58-59 11.924282710364704 1101 60-61 11.889874778571443 1101 62-63 12.081291016567672 1101 64-65 11.781794229146296 1101 66-67 11.649626146379195 1101 68-69 11.628811331692827 1101 70-71 11.394932498267512 1101 72-73 11.278807401765086 1101 74-75 11.193784169241574 1101 76-77 11.024313431787146 1101 78-79 11.028199829316637 1101 80-81 10.630042980176384 1101 82-83 10.468608759253435 1101 84-85 10.19923841439903 1101 86-87 10.22385948984219 1101 88-89 10.146010813573898 1101 90-91 9.81241091902163 1101 92-93 9.804278500075746 1101 94-95 9.582207114434281 1101 96-97 9.537805112835043 1101 98-99 9.473263464272087 1101 100-101 8.984617924695945 1102 1 8.07178330085394 1102 2 9.634717835462741 1102 3 8.973455241762899 1102 4 11.020479449159517 1102 5 11.96382136118676 1102 6 10.763104492831186 1102 7 10.500611612698911 1102 8 9.840260809795598 1102 9 9.681459657443384 1102 10-11 8.751711281311469 1102 12-13 8.792750784985802 1102 14-15 9.550555838396392 1102 16-17 10.71170797044525 1102 18-19 12.080496171237058 1102 20-21 12.254860793427596 1102 22-23 12.339502242813825 1102 24-25 12.167744397145075 1102 26-27 12.216533817262544 1102 28-29 12.036484788670716 1102 30-31 12.032417653431342 1102 32-33 11.945037728408352 1102 34-35 11.7675963452265 1102 36-37 12.144024403600614 1102 38-39 12.03649088178031 1102 40-41 11.455894118673239 1102 42-43 11.411900989684199 1102 44-45 11.5064663109053 1102 46-47 11.296676696028133 1102 48-49 11.023528671648123 1102 50-51 13.30763752439979 1102 52-53 12.01991180408583 1102 54-55 11.469120317332372 1102 56-57 11.151640787820025 1102 58-59 11.369156487508942 1102 60-61 11.330609742187537 1102 62-63 11.308842057503877 1102 64-65 11.13245446588121 1102 66-67 11.238796304866664 1102 68-69 11.036821132740467 1102 70-71 10.630801999966959 1102 72-73 10.576605710555086 1102 74-75 10.26817743543998 1102 76-77 10.1703957190112 1102 78-79 9.867999377726974 1102 80-81 9.525727348314884 1102 82-83 9.471836298559172 1102 84-85 9.401323472088318 1102 86-87 9.36356597092984 1102 88-89 9.226663012894118 1102 90-91 8.845820674852831 1102 92-93 8.857474042998799 1102 94-95 8.6130887728432 1102 96-97 8.336566905513811 1102 98-99 8.13758668329978 1102 100-101 7.605902416604886 1103 1 7.9945730936328765 1103 2 9.67127184264158 1103 3 8.894834905745043 1103 4 11.12708367803775 1103 5 11.942367797406703 1103 6 10.898565461672622 1103 7 10.428762911223934 1103 8 9.702516865054552 1103 9 9.475520526746315 1103 10-11 8.850149771040908 1103 12-13 8.691740883921046 1103 14-15 9.539789467328916 1103 16-17 10.922257355116326 1103 18-19 12.007009231895218 1103 20-21 12.268548280231201 1103 22-23 12.481217359092899 1103 24-25 12.360794422783773 1103 26-27 12.277529428519443 1103 28-29 11.874993935655905 1103 30-31 11.696634504093414 1103 32-33 11.85208328235521 1103 34-35 11.847842665318197 1103 36-37 12.201778415303924 1103 38-39 11.825140287565617 1103 40-41 11.490019047035677 1103 42-43 11.213775065936716 1103 44-45 11.148281342727447 1103 46-47 11.098338687401462 1103 48-49 11.3061737188535 1103 50-51 13.306943771194955 1103 52-53 11.805247972758345 1103 54-55 11.359343298661368 1103 56-57 11.052249330033401 1103 58-59 11.241684991716706 1103 60-61 11.247220795485877 1103 62-63 11.167836718620517 1103 64-65 10.887744334068099 1103 66-67 10.62843958752585 1103 68-69 10.55205636786248 1103 70-71 10.374291465475363 1103 72-73 10.403814975176687 1103 74-75 10.212279402079503 1103 76-77 9.968574805368394 1103 78-79 9.650197632786846 1103 80-81 9.227394618245206 1103 82-83 9.101412618952502 1103 84-85 8.948371439089794 1103 86-87 8.774297088777395 1103 88-89 8.447506526301655 1103 90-91 8.474578818912192 1103 92-93 8.308552188264692 1103 94-95 8.016348847346263 1103 96-97 7.9288766135342055 1103 98-99 7.734353944181148 1103 100-101 7.0091615485084855 1104 1 8.05773198687822 1104 2 9.742011030654464 1104 3 9.077304160493537 1104 4 11.207322646574546 1104 5 11.947989547114695 1104 6 11.001260739588862 1104 7 10.475527126950325 1104 8 9.744278700023418 1104 9 9.658963474124135 1104 10-11 8.823597814727815 1104 12-13 8.643547748686576 1104 14-15 9.572293317460229 1104 16-17 10.804507505773632 1104 18-19 11.954330786678682 1104 20-21 12.214351637099313 1104 22-23 12.341996517792712 1104 24-25 12.242463333810402 1104 26-27 12.280384477585365 1104 28-29 12.254022158792406 1104 30-31 11.931579002683566 1104 32-33 11.915114302881804 1104 34-35 12.057174490838428 1104 36-37 12.272204198907946 1104 38-39 11.903306788075241 1104 40-41 11.4973315065814 1104 42-43 11.329307369840421 1104 44-45 11.451016516931947 1104 46-47 11.308024836600563 1104 48-49 11.400003929922534 1104 50-51 13.397141467068057 1104 52-53 12.123387392151102 1104 54-55 11.404686964730363 1104 56-57 11.162740658868099 1104 58-59 11.20569323880957 1104 60-61 11.075981090402212 1104 62-63 11.081033462190614 1104 64-65 10.917317806477353 1104 66-67 10.841509946350651 1104 68-69 10.859295916708927 1104 70-71 10.569425234288925 1104 72-73 10.547878240088043 1104 74-75 10.100989217496398 1104 76-77 10.11234280054736 1104 78-79 9.819744768065952 1104 80-81 9.61729155748381 1104 82-83 9.493140695523614 1104 84-85 9.063939547354746 1104 86-87 9.050410557913104 1104 88-89 8.79911046868732 1104 90-91 8.582057001501415 1104 92-93 8.284836216357625 1104 94-95 8.311291690565936 1104 96-97 7.912565634522066 1104 98-99 7.764457042141878 1104 100-101 7.135609532676446 1105 1 8.08017666510731 1105 2 9.553338933452938 1105 3 8.99836533243974 1105 4 11.05565373070727 1105 5 11.485461274347507 1105 6 10.8221013185935 1105 7 10.415103186441002 1105 8 9.782298078306507 1105 9 9.54617079633136 1105 10-11 8.790036158234383 1105 12-13 8.60778805075557 1105 14-15 9.557435409243837 1105 16-17 10.827737939305923 1105 18-19 11.625909973414622 1105 20-21 12.065530055951854 1105 22-23 12.530987514679882 1105 24-25 12.47945357886059 1105 26-27 12.616510380533484 1105 28-29 12.305310581133686 1105 30-31 11.899909334077808 1105 32-33 11.81458731833412 1105 34-35 11.776570488072679 1105 36-37 12.003477012067439 1105 38-39 11.851413019758233 1105 40-41 11.688881588931888 1105 42-43 11.413714595048056 1105 44-45 11.478732505548013 1105 46-47 11.232887675930112 1105 48-49 10.988537018413027 1105 50-51 13.12398608201303 1105 52-53 11.664205988487652 1105 54-55 11.549830381694289 1105 56-57 11.385014415880509 1105 58-59 11.479077487914633 1105 60-61 11.261303839421178 1105 62-63 11.415701892158442 1105 64-65 10.900079982585705 1105 66-67 10.895645928782546 1105 68-69 10.655518228778977 1105 70-71 10.701492862657883 1105 72-73 10.700821402473117 1105 74-75 10.25066224953601 1105 76-77 10.020189242798796 1105 78-79 10.007327648178602 1105 80-81 9.796690885698524 1105 82-83 9.711445672702567 1105 84-85 9.623085297357225 1105 86-87 9.224574860440054 1105 88-89 9.069685583423599 1105 90-91 8.894210324864346 1105 92-93 8.704848512588619 1105 94-95 8.455742317682732 1105 96-97 8.286740213957613 1105 98-99 8.022084040842842 1105 100-101 7.553406884659664 1106 1 7.762734993579382 1106 2 9.246693922704893 1106 3 8.548702690630765 1106 4 10.534209576606628 1106 5 11.365099117066407 1106 6 10.396904803784633 1106 7 9.6114435226538 1106 8 9.124635399205662 1106 9 8.80273150082796 1106 10-11 8.338024128944287 1106 12-13 8.103048654297769 1106 14-15 8.657673278067037 1106 16-17 10.253176185309702 1106 18-19 11.389309073615195 1106 20-21 11.600053332734738 1106 22-23 11.713915099205895 1106 24-25 11.56165616957971 1106 26-27 11.988637242130054 1106 28-29 11.772920265830965 1106 30-31 11.321271779039012 1106 32-33 11.304669347118121 1106 34-35 11.358628239852699 1106 36-37 11.73285827343813 1106 38-39 11.30274613733149 1106 40-41 11.067261164177548 1106 42-43 11.009171114065573 1106 44-45 10.583308217356478 1106 46-47 10.62006280385452 1106 48-49 10.633751539865173 1106 50-51 12.59245281367409 1106 52-53 11.388257509862555 1106 54-55 10.857073061168048 1106 56-57 10.585311352353823 1106 58-59 10.550352563364061 1106 60-61 10.500387311933075 1106 62-63 10.77120190947252 1106 64-65 10.51872105371461 1106 66-67 10.367068706653551 1106 68-69 10.188573804312316 1106 70-71 10.035530079938933 1106 72-73 9.798561049319154 1106 74-75 9.529923044864937 1106 76-77 9.12747127850842 1106 78-79 8.720754983648227 1106 80-81 8.381207902775532 1106 82-83 8.310787870844898 1106 84-85 8.073296329356307 1106 86-87 7.968098062639207 1106 88-89 7.8852520068940315 1106 90-91 7.582778969864435 1106 92-93 7.46899711267865 1106 94-95 7.50961874043397 1106 96-97 7.105792094932637 1106 98-99 6.652228320403971 1106 100-101 6.125320583293449 1107 1 7.6521066507412705 1107 2 8.888621151366976 1107 3 8.523072567307832 1107 4 10.516654527736307 1107 5 11.572283771944669 1107 6 10.409183948372398 1107 7 10.285930149529733 1107 8 8.898733065441828 1107 9 6.373808720720497 1107 10-11 8.082076335239588 1107 12-13 7.920451520435424 1107 14-15 8.735551772822134 1107 16-17 9.88702833951918 1107 18-19 11.331820673340943 1107 20-21 11.657978660441387 1107 22-23 11.2709045651136 1107 24-25 11.436884045062694 1107 26-27 11.600045543618407 1107 28-29 11.754826863900252 1107 30-31 11.214564267519012 1107 32-33 11.525887278460502 1107 34-35 11.6383536997227 1107 36-37 12.075996453233433 1107 38-39 11.67191438517498 1107 40-41 11.193254603843922 1107 42-43 11.006886158542258 1107 44-45 10.429358729976666 1107 46-47 10.875847829357088 1107 48-49 11.209010241652564 1107 50-51 12.918375654016867 1107 52-53 11.328979766631491 1107 54-55 11.17799238483185 1107 56-57 10.897973566922612 1107 58-59 10.950297510064676 1107 60-61 10.919906297570495 1107 62-63 11.039394301044542 1107 64-65 10.96874855821897 1107 66-67 11.206246414983564 1107 68-69 10.835441435452555 1107 70-71 10.463640126302405 1107 72-73 10.362705229614058 1107 74-75 10.298130052357145 1107 76-77 10.100988116934293 1107 78-79 9.874856388016703 1107 80-81 9.610515081584044 1107 82-83 9.357104836119245 1107 84-85 9.220332265032084 1107 86-87 8.898330960683865 1107 88-89 9.182074328681676 1107 90-91 8.845934848210376 1107 92-93 8.44744150928917 1107 94-95 8.353071826873405 1107 96-97 8.2474380778577 1107 98-99 7.927561031896335 1107 100-101 7.3660007196378405 1108 1 6.990649253297107 1108 2 8.465993421213113 1108 3 8.24536099910111 1108 4 9.823567159289297 1108 5 11.574297365019177 1108 6 10.089616773991363 1108 7 9.396439502255568 1108 8 6.730623147571055 1108 9 7.595643961946479 1108 10-11 6.423290956426879 1108 12-13 5.41708936611985 1108 14-15 7.8371761240857865 1108 16-17 9.19578764556066 1108 18-19 9.26579688546056 1108 20-21 9.400566198009074 1108 22-23 10.40771548643372 1108 24-25 10.223597545825502 1108 26-27 8.770530638096965 1108 28-29 10.499137758795275 1108 30-31 10.502142089456076 1108 32-33 11.037279802269516 1108 34-35 10.633758373795445 1108 36-37 10.484295830305602 1108 38-39 10.059117939527894 1108 40-41 10.453109626904226 1108 42-43 9.665703467691337 1108 44-45 9.3212390876669 1108 46-47 10.040378246415727 1108 48-49 10.691101727131091 1108 50-51 12.480548202912118 1108 52-53 11.287767391578207 1108 54-55 10.5137429547333 1108 56-57 10.37338214310267 1108 58-59 10.527270981019957 1108 60-61 10.502439831029967 1108 62-63 10.633590555021517 1108 64-65 10.384381562823986 1108 66-67 9.89822547044458 1108 68-69 10.313079189529947 1108 70-71 10.111593633970969 1108 72-73 9.95409623838367 1108 74-75 9.707707964139573 1108 76-77 9.537902360418745 1108 78-79 9.369499138200801 1108 80-81 9.375609762738712 1108 82-83 9.266929822642439 1108 84-85 8.85493977293319 1108 86-87 8.897428983733604 1108 88-89 8.628353849929223 1108 90-91 8.398938325754322 1108 92-93 8.214566739628012 1108 94-95 7.958161610575106 1108 96-97 7.852672318805338 1108 98-99 7.532211498211375 1108 100-101 6.910955330114161 1109 1 6.797949685531421 1109 2 8.686426911091136 1109 3 8.328132668252188 1109 4 9.797110839532074 1109 5 10.962824722940177 1109 6 9.769179009855595 1109 7 8.640418809505249 1109 8 8.77481029060375 1109 9 7.818348819106127 1109 10-11 6.31853891680451 1109 12-13 6.597278715738103 1109 14-15 8.139731462366576 1109 16-17 7.309532990626838 1109 18-19 6.705910861214829 1109 20-21 9.218661468542876 1109 22-23 10.496879508403406 1109 24-25 9.99201191641433 1109 26-27 10.37714531910376 1109 28-29 10.555325882235838 1109 30-31 8.259232682349054 1109 32-33 7.458854852892756 1109 34-35 9.261951377904069 1109 36-37 7.500036786243353 1109 38-39 7.879330637279104 1109 40-41 8.363123478170742 1109 42-43 7.2620275392424105 1109 44-45 8.005259908644955 1109 46-47 7.426344722877925 1109 48-49 8.144688296878961 1109 50-51 10.22995848891339 1109 52-53 10.249411605034638 1109 54-55 10.228860002610329 1109 56-57 9.330052856957142 1109 58-59 9.20217327415924 1109 60-61 9.488893105378544 1109 62-63 9.997731268929417 1109 64-65 10.047744361821671 1109 66-67 9.480851881617486 1109 68-69 9.35059181194736 1109 70-71 8.785910220228164 1109 72-73 8.24595023775322 1109 74-75 7.395503155065324 1109 76-77 7.097363630300723 1109 78-79 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-19.55816909388641 2313 60-61 -19.300930955486887 2313 62-63 -18.890285849472864 2313 64-65 -18.602041658149798 2313 66-67 -18.337027338544186 2313 68-69 -17.94113429361612 2313 70-71 -17.633490635691953 2313 72-73 -17.266175297196853 2313 74-75 -16.866027990916606 2313 76-77 -16.524129474787248 2313 78-79 -16.219452172660624 2313 80-81 -15.865261023778139 2313 82-83 -15.454278042526099 2313 84-85 -15.127504047291527 2313 86-87 -14.694331315891866 2313 88-89 -14.342619932842315 2313 90-91 -13.932522348402983 2313 92-93 -13.444609290334533 2313 94-95 -12.899750374443622 2313 96-97 -12.324528055726498 2313 98-99 -11.66909936321185 2313 100-101 -10.53169448261992 2314 1 -20.335193443332106 2314 2 -18.8846620094985 2314 3 -19.696118401647954 2314 4 -21.126807372545912 2314 5 -20.397612747340375 2314 6 -21.550364499416876 2314 7 -21.90927210823132 2314 8 -22.509545391754788 2314 9 -24.142183753409327 2314 10-11 -24.734480579153644 2314 12-13 -24.747704641370785 2314 14-15 -24.807486797262527 2314 16-17 -23.461983890019862 2314 18-19 -22.22086041791023 2314 20-21 -21.82532331509954 2314 22-23 -21.4562826409071 2314 24-25 -21.321112192002214 2314 26-27 -21.084338275760715 2314 28-29 -20.960365986523083 2314 30-31 -21.1088129667237 2314 32-33 -21.04066963593273 2314 34-35 -20.79320791833939 2314 36-37 -20.261256604151324 2314 38-39 -20.27140640504139 2314 40-41 -20.25171247047405 2314 42-43 -20.16973660721503 2314 44-45 -19.81499686738928 2314 46-47 -19.480455086917605 2314 48-49 -19.366248460134827 2314 50-51 -16.701567901956796 2314 52-53 -18.873983544751383 2314 54-55 -19.755685884218014 2314 56-57 -19.80475456101718 2314 58-59 -19.55816909388641 2314 60-61 -19.300930955486887 2314 62-63 -18.890285849472864 2314 64-65 -18.602041658149798 2314 66-67 -18.337027338544186 2314 68-69 -17.94113429361612 2314 70-71 -17.633490635691953 2314 72-73 -17.266175297196853 2314 74-75 -16.866027990916606 2314 76-77 -16.524129474787248 2314 78-79 -16.219452172660624 2314 80-81 -15.865261023778139 2314 82-83 -15.454278042526099 2314 84-85 -15.127504047291527 2314 86-87 -14.694331315891866 2314 88-89 -14.342619932842315 2314 90-91 -13.932522348402983 2314 92-93 -13.444609290334533 2314 94-95 -12.899750374443622 2314 96-97 -12.324528055726498 2314 98-99 -11.66909936321185 2314 100-101 -10.53169448261992 2315 1 -20.335193443332106 2315 2 -18.8846620094985 2315 3 -19.696118401647954 2315 4 -21.126807372545912 2315 5 -20.397612747340375 2315 6 -21.550364499416876 2315 7 -21.90927210823132 2315 8 -22.509545391754788 2315 9 -24.142183753409327 2315 10-11 -24.734480579153644 2315 12-13 -24.747704641370785 2315 14-15 -24.807486797262527 2315 16-17 -23.461983890019862 2315 18-19 -22.22086041791023 2315 20-21 -21.82532331509954 2315 22-23 -21.4562826409071 2315 24-25 -21.321112192002214 2315 26-27 -21.084338275760715 2315 28-29 -20.960365986523083 2315 30-31 -21.1088129667237 2315 32-33 -21.04066963593273 2315 34-35 -20.79320791833939 2315 36-37 -20.261256604151324 2315 38-39 -20.27140640504139 2315 40-41 -20.25171247047405 2315 42-43 -20.16973660721503 2315 44-45 -19.81499686738928 2315 46-47 -19.480455086917605 2315 48-49 -19.366248460134827 2315 50-51 -16.701567901956796 2315 52-53 -18.873983544751383 2315 54-55 -19.755685884218014 2315 56-57 -19.80475456101718 2315 58-59 -19.55816909388641 2315 60-61 -19.300930955486887 2315 62-63 -18.890285849472864 2315 64-65 -18.602041658149798 2315 66-67 -18.337027338544186 2315 68-69 -17.94113429361612 2315 70-71 -17.633490635691953 2315 72-73 -17.266175297196853 2315 74-75 -16.866027990916606 2315 76-77 -16.524129474787248 2315 78-79 -16.219452172660624 2315 80-81 -15.865261023778139 2315 82-83 -15.454278042526099 2315 84-85 -15.127504047291527 2315 86-87 -14.694331315891866 2315 88-89 -14.342619932842315 2315 90-91 -13.932522348402983 2315 92-93 -13.444609290334533 2315 94-95 -12.899750374443622 2315 96-97 -12.324528055726498 2315 98-99 -11.66909936321185 2315 100-101 -10.53169448261992 2316 1 -20.335193443332106 2316 2 -18.8846620094985 2316 3 -19.696118401647954 2316 4 -21.126807372545912 2316 5 -20.397612747340375 2316 6 -21.550364499416876 2316 7 -21.90927210823132 2316 8 -22.509545391754788 2316 9 -24.142183753409327 2316 10-11 -24.734480579153644 2316 12-13 -24.747704641370785 2316 14-15 -24.807486797262527 2316 16-17 -23.461983890019862 2316 18-19 -22.22086041791023 2316 20-21 -21.82532331509954 2316 22-23 -21.4562826409071 2316 24-25 -21.321112192002214 2316 26-27 -21.084338275760715 2316 28-29 -20.960365986523083 2316 30-31 -21.1088129667237 2316 32-33 -21.04066963593273 2316 34-35 -20.79320791833939 2316 36-37 -20.261256604151324 2316 38-39 -20.27140640504139 2316 40-41 -20.25171247047405 2316 42-43 -20.16973660721503 2316 44-45 -19.81499686738928 2316 46-47 -19.480455086917605 2316 48-49 -19.366248460134827 2316 50-51 -16.701567901956796 2316 52-53 -18.873983544751383 2316 54-55 -19.755685884218014 2316 56-57 -19.80475456101718 2316 58-59 -19.55816909388641 2316 60-61 -19.300930955486887 2316 62-63 -18.890285849472864 2316 64-65 -18.602041658149798 2316 66-67 -18.337027338544186 2316 68-69 -17.94113429361612 2316 70-71 -17.633490635691953 2316 72-73 -17.266175297196853 2316 74-75 -16.866027990916606 2316 76-77 -16.524129474787248 2316 78-79 -16.219452172660624 2316 80-81 -15.865261023778139 2316 82-83 -15.454278042526099 2316 84-85 -15.127504047291527 2316 86-87 -14.694331315891866 2316 88-89 -14.342619932842315 2316 90-91 -13.932522348402983 2316 92-93 -13.444609290334533 2316 94-95 -12.899750374443622 2316 96-97 -12.324528055726498 2316 98-99 -11.66909936321185 2316 100-101 -10.53169448261992 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 445100.0 3 17714.0 4 77542.0 5 39176.0 6 15230.0 7 15165.0 8 16686.0 9 13947.0 10 13038.0 11 14569.0 12 15917.0 13 17098.0 14 18382.0 15 19780.0 16 21785.0 17 22529.0 18 20823.0 19 20344.0 20 21737.0 21 23244.0 22 24981.0 23 27897.0 24 30749.0 25 33566.0 26 37367.0 27 41907.0 28 47165.0 29 53143.0 30 60481.0 31 69312.0 32 80303.0 33 94374.0 34 109636.0 35 127945.0 36 136175.0 37 104283.0 38 32861.0 39 1136.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.93759052348438 20.819948789571693 14.399570660045521 22.842890026898406 2 14.520530053294944 21.018282693197726 42.254344943510766 22.206842309996564 3 18.113871941069025 26.854681928626356 30.75460267680533 24.27684345349929 4 11.857384307666273 17.556195653951526 40.474238488716786 30.11218154966542 5 12.224935422125588 38.48885915519132 36.095073497983684 13.191131924699413 6 28.71570538664749 39.32122526138973 18.527443069834963 13.435626282127824 7 25.260352619789973 33.65755101932877 23.720450837121604 17.361645523759655 8 24.061608276451587 37.018327865143114 21.199859209815088 17.72020464859021 9 25.273710434064483 16.960280718106524 21.794889367870248 35.97111947995874 10-11 23.451077444044113 27.617198383536262 29.53776114533647 19.39396302708315 12-13 24.180887173662498 25.151349787068668 29.804605062964434 20.863157976304404 14-15 21.407689690115 26.809166514697747 27.54438275864014 24.238761036547114 16-17 20.72848996821068 29.898937656889558 28.145202999741404 21.227369375158357 18-19 20.590169935144196 28.64970434394906 30.424947948847443 20.3351777720593 20-21 21.368133480650776 27.930049466517083 30.46674400714544 20.2350730456867 22-23 20.93960813352404 27.731247114074307 30.228910218147824 21.100234534253826 24-25 20.8556787896911 27.90292612953415 30.480288919033484 20.761106161741267 26-27 20.64420803574539 28.17458320680311 30.240752854539448 20.940455902912053 28-29 20.743371306451063 28.220170921238395 30.35423830171458 20.68221947059596 30-31 21.058653646755634 28.012126713562996 30.25214826108647 20.677071378594903 32-33 20.817424620965927 28.269242526135603 30.050449075669516 20.86288377722896 34-35 20.940169517481074 28.225359708637672 30.108794801286358 20.725675972594896 36-37 21.017458329218663 28.03207883534909 30.137958395919632 20.812504439512615 38-39 20.95829664534396 28.269737154923213 30.06137205039845 20.710594149334373 40-41 21.120978784224842 28.22446927206592 30.009756488064564 20.644795455644672 42-43 20.973626001496214 28.278873480764727 29.799710875623227 20.94778964211583 44-45 20.982975355628188 28.49072212275019 29.66396511085622 20.862337410765402 46-47 21.12143671410207 28.37340568185362 29.4102971902579 21.094860413786414 48-49 21.10150802120213 28.572252748864567 29.276361035908156 21.04987819402515 50-51 21.385061162192383 28.705436877573327 28.84312606839184 21.06637589184245 52-53 20.945084290952952 28.84934060511454 29.067139267941055 21.138435835991444 54-55 20.93435989674149 28.793061468134905 29.161234075307497 21.111344559816104 56-57 20.899556712471473 28.953230372901253 29.116392755307572 21.030820159319706 58-59 20.89792914545044 28.845876306012247 29.15231651667406 21.10387803186325 60-61 20.940479802429618 28.953659993984914 29.16783148221509 20.93802872137038 62-63 20.836407388309336 28.832454087501485 29.147891793264847 21.18324673092433 64-65 20.703249275900955 28.813385624781297 29.235797005143855 21.247568094173904 66-67 20.81715263178512 28.845109227896533 28.95773213376337 21.38000600655498 68-69 20.836093908485115 28.78737686512392 29.062343374553514 21.314185851837454 70-71 20.864606070510053 28.566453836965444 29.088898226937935 21.480041865586568 72-73 20.859567009228254 28.81239202578241 28.94301291515662 21.385028049832712 74-75 20.74023404007645 28.984614286983852 28.68865334957186 21.586498323367834 76-77 20.83649793646421 28.902277484417276 28.749668521231374 21.511556057887134 78-79 20.704504491886212 28.946324649635425 28.7664072741117 21.58276358436667 80-81 20.748728938854597 29.00860294039403 28.576440538651966 21.66622758209941 82-83 20.992901744722918 28.808283872843543 28.734716521232347 21.464097861201193 84-85 21.0042953299737 28.913198696970305 28.49803365992732 21.58447231312868 86-87 20.804652149117743 29.04273159273289 28.64036073922371 21.512255518925663 88-89 20.882221667460986 28.964935307274686 28.553687018839184 21.599156006425144 90-91 20.894725552046438 28.901712668272143 28.688768025247736 21.514793754433683 92-93 20.87072310309812 28.792756982983985 28.480544747703558 21.85597516621434 94-95 20.68986214888022 28.92772704239911 28.580034901896862 21.802375906823816 96-97 20.749191707503293 28.736259027555956 28.536223190009114 21.978326074931637 98-99 20.54240687909549 28.731533745519744 28.299486654057297 22.42657272132746 100-101 20.746037420293664 28.844251796234726 28.287507452731997 22.122203330739616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 118049.0 1 107102.0 2 71172.0 3 36169.5 4 22465.0 5 17694.0 6 16433.0 7 16757.5 8 17400.5 9 17256.0 10 15795.0 11 13386.5 12 10786.5 13 8297.0 14 6474.5 15 5426.5 16 5059.5 17 5447.5 18 6237.5 19 7254.0 20 8427.5 21 9538.5 22 10695.5 23 12050.5 24 13729.5 25 15830.0 26 18365.5 27 21098.5 28 23867.0 29 27018.5 30 30283.5 31 33481.0 32 37172.0 33 40661.0 34 43897.5 35 46873.0 36 50674.0 37 54433.0 38 56045.0 39 56742.0 40 56894.0 41 56639.5 42 56636.5 43 57098.5 44 57390.0 45 57582.0 46 57294.5 47 55943.0 48 54541.0 49 52571.0 50 49302.0 51 45624.5 52 42197.0 53 38409.0 54 34258.0 55 29729.0 56 24643.0 57 19840.0 58 15889.0 59 12781.0 60 9858.5 61 7165.5 62 5103.0 63 3416.5 64 2128.0 65 1338.0 66 845.0 67 515.5 68 307.5 69 197.5 70 125.5 71 87.5 72 57.5 73 37.5 74 33.5 75 22.5 76 14.0 77 14.5 78 11.0 79 7.5 80 5.0 81 4.0 82 3.5 83 2.0 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.012498695216095 2 28.96105919710028 3 22.755481731260403 4 29.08122538244666 5 32.16258288214284 6 24.830025107320054 7 23.72679564739217 8 19.77053956785557 9 11.706445556851515 10-11 15.29791683370422 12-13 15.401921347878334 14-15 19.533484915185266 16-17 22.78039238823108 18-19 26.711334399348086 20-21 28.77924669971615 22-23 28.359774432488337 24-25 26.897836554825883 26-27 28.347142611494096 28-29 25.331541177971516 30-31 27.171652075778823 32-33 26.17815052995658 34-35 24.264038844488418 36-37 27.234281703223306 38-39 27.353691492102968 40-41 24.41065369295447 42-43 25.247505530518833 44-45 23.98704645837525 46-47 27.233903504989946 48-49 22.646005949310343 50-51 28.273116610617688 52-53 21.824660239313758 54-55 22.95769172003044 56-57 25.069197670097175 58-59 24.35382310508818 60-61 23.87938602794532 62-63 23.721954710005157 64-65 22.255377600680152 66-67 22.76385251882545 68-69 23.736780080752887 70-71 22.238509959472278 72-73 22.094744204364204 74-75 19.426328749066478 76-77 21.65527785719941 78-79 20.794145693053306 80-81 21.810061787506044 82-83 22.647443102597112 84-85 19.629143855009893 86-87 21.171663169593668 88-89 20.010165968512727 90-91 21.131977568306386 92-93 21.116900065403083 94-95 20.18897809324553 96-97 19.092682267595926 98-99 18.497826872951112 100-101 20.159226498887843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983087.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.37839558134145 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7950918371388 64.5567967518157 2 8.909209992569405 13.253054902891344 3 2.109021897514515 4.7059699494913785 4 0.8127221485604066 2.417958778533747 5 0.4196231378115449 1.5605447869615425 6 0.23899738151088762 1.0665745070952948 7 0.1600517972701219 0.8333077123603254 8 0.10353394723017671 0.6160551106547051 9 0.07619044983532781 0.5100231075635125 >10 0.35771003341957497 4.466031675771738 >50 0.012039702533337507 0.6085466567233302 >100 0.005531340160286172 0.645399903152176 >500 0.0 0.0 >1k 2.072508342070613E-4 0.27561992341813885 >5k 0.0 0.0 >10k+ 6.908361140235377E-5 4.48411623356714 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 87268 4.400613790519528 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2489 0.12551138704454218 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.521321555736082E-5 0.0 16-17 0.0 0.0 0.0 5.042643111472164E-5 0.0 18-19 0.0 0.0 0.0 5.042643111472164E-5 0.0 20-21 0.0 0.0 0.0 5.042643111472164E-5 0.0 22-23 0.0 0.0 0.0 5.042643111472164E-5 0.0 24-25 0.0 0.0 0.0 5.042643111472164E-5 0.0 26-27 0.0 0.0 0.0 7.563964667208246E-5 2.521321555736082E-5 28-29 0.0 0.0 0.0 5.546907422619382E-4 5.042643111472164E-5 30-31 0.0 0.0 0.0 0.001411940071212206 5.042643111472164E-5 32-33 0.0 0.0 0.0 0.0043114598603087 5.042643111472164E-5 34-35 0.0 0.0 0.0 0.008673346151732122 5.042643111472164E-5 36-37 0.0 0.0 0.0 0.015153142549973854 5.042643111472164E-5 38-39 0.0 0.0 0.0 0.025919185592966923 5.042643111472164E-5 40-41 0.0 0.0 0.0 0.036634802204845274 5.042643111472164E-5 42-43 0.0 0.0 0.0 0.04752691132562515 5.042643111472164E-5 44-45 0.0 0.0 0.0 0.061343753451058886 5.042643111472164E-5 46-47 0.0 0.0 0.0 0.07818618144337591 5.042643111472164E-5 48-49 0.0 0.0 0.0 0.0976255706381011 5.042643111472164E-5 50-51 0.0 0.0 0.0 0.11885509813739892 5.042643111472164E-5 52-53 0.0 0.0 0.0 0.14114358069010588 5.042643111472164E-5 54-55 0.0 0.0 0.0 0.1657768922896474 5.042643111472164E-5 56-57 0.0 0.0 0.0 0.1973942645985779 5.042643111472164E-5 58-59 0.0 0.0 0.0 0.23297011175001398 5.042643111472164E-5 60-61 0.0 0.0 0.0 0.27275656589952935 5.042643111472164E-5 62-63 0.0 0.0 0.0 0.3123161011090285 5.042643111472164E-5 64-65 0.0 0.0 0.0 0.3539935464253459 5.042643111472164E-5 66-67 0.0 0.0 0.0 0.40232728064880663 5.042643111472164E-5 68-69 0.0 0.0 0.0 0.4592082949462126 5.042643111472164E-5 70-71 0.0 0.0 0.0 0.5222917602707294 5.042643111472164E-5 72-73 0.0 0.0 0.0 0.5873670696242776 5.042643111472164E-5 74-75 2.521321555736082E-5 0.0 0.0 0.655871376293627 5.042643111472164E-5 76-77 5.042643111472164E-5 0.0 0.0 0.7349400202815106 5.042643111472164E-5 78-79 5.042643111472164E-5 0.0 0.0 0.823640112612306 5.042643111472164E-5 80-81 5.042643111472164E-5 0.0 0.0 0.9233331669261107 5.042643111472164E-5 82-83 5.042643111472164E-5 0.0 0.0 1.0266064978490608 5.042643111472164E-5 84-85 5.042643111472164E-5 0.0 0.0 1.1325524296210907 5.042643111472164E-5 86-87 5.042643111472164E-5 0.0 0.0 1.2443730406179858 5.042643111472164E-5 88-89 5.042643111472164E-5 2.521321555736082E-5 0.0 1.3688002593935615 5.042643111472164E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1660 0.0 58.321007 1 GTATCAA 3430 0.0 47.042213 1 ATCAACG 4265 0.0 37.553215 3 TCAACGC 4290 0.0 37.321705 4 TATCAAC 4410 0.0 36.23159 2 CAACGCA 4395 0.0 35.614716 5 AACGCAG 4805 0.0 33.200005 6 ACGCAGA 5640 0.0 28.687021 7 TATCGGC 110 5.6795037E-5 27.933794 2 CGCAGAG 5680 0.0 27.739597 8 GTACATG 4070 0.0 25.423086 1 TACATGG 4020 0.0 25.096407 2 ACATGGG 4000 0.0 24.679428 3 GCAGAGT 6370 0.0 24.259357 9 AGTACTT 4515 0.0 21.534325 12-13 GAGTACT 4170 0.0 21.531263 12-13 AGAGTAC 5715 0.0 20.966742 10-11 CAGAGTA 6050 0.0 20.108728 10-11 GTGGTAT 910 0.0 19.701439 1 ACGGTAG 130 0.0054153847 19.35774 5 >>END_MODULE