FastQCFastQC Report
Sat 18 Jun 2016
SRR1930976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983087
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.3213172190630063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54400.27431978526408574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34070.17180285080785662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.061.0283241
GTATCAA57450.041.204951
ATCAACG73400.031.8592283
TCAACGC73750.031.708034
CAACGCA74200.031.6463415
TATCAAC77050.030.413222
AACGCAG77750.030.3836676
ACGCAGA88900.026.3594557
CGCAGAG89600.026.1005788
CGCGACA500.001666341423.72056634-35
GTGGTAT13050.023.3121
GCAGAGT102850.022.1846229
TACATGG57900.021.4264432
GTACATG59450.021.188691
ACATGGG56200.021.1431583
CAGAGTA97950.020.87296310-11
TAACGCA1604.9675513E-520.7942964
GAGTACT74450.020.67574912-13
TGGTATC13150.020.6033232
GCGTGCG3950.020.4156449
Produced by FastQC (version 0.11.5)