FastQCFastQC Report
Sat 18 Jun 2016
SRR1930975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050142
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN863084.209854732013685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26250.1280399113817482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.067.4984741
GTATCAA38250.046.9722061
TATCAAC49300.036.126812
CAACGCA49750.035.6207545
ATCAACG49500.035.4050753
TCAACGC51050.034.623964
AACGCAG55500.032.511616
ACGCAGA62750.029.1598227
CGCAGAG65550.027.786928
GTACATG43200.027.1752681
GTGGTAT7900.027.1363811
TACATGG45100.025.6862282
ACATGGG44900.025.2759423
GCAGAGT71250.025.0552859
CATGGGT10150.024.7362464
GAGTACT44950.022.54407112-13
CCGATCG2004.539652E-822.039379
ACGGGCG3207.2759576E-1221.9633965
CAGAGTA67250.021.91329810-11
GCGTCGG3650.021.7374619
Produced by FastQC (version 0.11.5)