FastQCFastQC Report
Sat 18 Jun 2016
SRR1930975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050142
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64200.31314904040793273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53600.26144530476425537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.16257410462299685No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA22870.11155324850668881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.061.2669871
GTATCAA54800.047.0912361
CAACGCA73250.034.390975
GTGGTAT10800.034.323511
TATCAAC74350.034.1310232
ATCAACG74700.033.6513823
TCAACGC75850.033.0150684
AACGCAG79100.032.327336
ACGCAGA91850.027.2200557
CGCAGAG93550.026.7254098
TGGTATC14050.025.3672832
GTACATG61150.025.1807921
ACATGGG61050.024.4405963
TACATGG64100.024.0943012
GCAGAGT104850.023.4379089
GGGCTAT12050.022.4411626
GTACTAG4900.022.3075871
CATGGGT13750.022.117424
TCTAACG1553.8948034E-521.4612662
AGAGTAC99100.021.35102710-11
Produced by FastQC (version 0.11.5)