##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930975_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2050142 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.337777090562508 31.0 31.0 34.0 30.0 34.0 2 31.50192425695391 31.0 31.0 34.0 30.0 34.0 3 31.564518945516944 31.0 31.0 34.0 30.0 34.0 4 35.34458881384801 37.0 35.0 37.0 33.0 37.0 5 35.21055468352924 37.0 35.0 37.0 33.0 37.0 6 35.231903936410255 37.0 35.0 37.0 32.0 37.0 7 35.11195029417475 37.0 35.0 37.0 32.0 37.0 8 35.11049185861272 37.0 35.0 37.0 32.0 37.0 9 36.61251854749573 39.0 35.0 39.0 32.0 39.0 10-11 36.54815154267363 38.0 35.0 39.0 32.0 39.0 12-13 36.42946708081684 38.0 35.0 39.0 32.0 39.0 14-15 37.47398155835059 39.0 36.0 41.0 32.0 41.0 16-17 37.42645460655896 39.0 36.0 41.0 32.0 41.0 18-19 37.45419878232825 39.0 36.0 41.0 32.0 41.0 20-21 37.43022312600786 39.0 36.0 41.0 32.0 41.0 22-23 37.32685443252223 39.0 36.0 41.0 31.5 41.0 24-25 37.194517501714515 39.0 36.0 40.5 31.0 41.0 26-27 36.91466298432011 39.0 36.0 40.0 31.0 41.0 28-29 36.731271297305256 39.0 35.0 40.0 30.0 41.0 30-31 36.48551661299558 38.0 35.0 40.0 30.0 41.0 32-33 36.3065168168839 38.0 35.0 40.0 30.0 41.0 34-35 36.37950688293787 38.0 35.0 40.0 30.0 41.0 36-37 36.441801836165496 38.0 35.0 40.0 30.0 41.0 38-39 36.412236323142494 38.5 35.0 40.0 30.0 41.0 40-41 36.282946986111206 38.0 35.0 40.0 30.0 41.0 42-43 36.12536009700791 38.0 35.0 40.0 30.0 41.0 44-45 35.92080255904225 38.0 35.0 40.0 29.0 41.0 46-47 35.62644587545643 38.0 34.5 40.0 27.5 41.0 48-49 35.369359537046705 38.0 34.0 40.0 27.0 41.0 50-51 35.075123089034804 38.0 34.0 40.0 26.5 41.0 52-53 34.83758198212612 38.0 34.0 40.0 26.0 41.0 54-55 34.65881046288501 38.0 33.0 40.0 26.0 41.0 56-57 34.44939033491339 38.0 33.0 40.0 25.5 41.0 58-59 34.20403001353077 37.0 33.0 40.0 25.0 41.0 60-61 33.87268101429072 37.0 33.0 40.0 24.0 41.0 62-63 33.58650815406933 36.5 32.0 39.5 23.0 41.0 64-65 33.24057748195003 36.0 32.0 39.0 23.0 41.0 66-67 32.88789264353396 35.5 31.5 39.0 22.0 40.0 68-69 32.51936548785401 35.0 31.0 38.5 22.0 40.0 70-71 32.12981710535173 35.0 31.0 38.0 21.0 40.0 72-73 31.7070234647161 35.0 31.0 37.0 20.0 39.5 74-75 31.19510111982487 34.5 30.0 36.5 18.0 39.0 76-77 29.847997358231773 33.5 28.5 35.0 17.5 37.5 78-79 30.57975642662801 34.0 30.0 36.0 18.0 38.0 80-81 30.568335266532756 34.0 30.0 35.5 18.0 37.0 82-83 30.331585568219175 34.0 30.0 35.0 18.0 37.0 84-85 30.024399773283996 34.0 30.0 35.0 16.0 36.5 86-87 29.716736938221842 34.0 29.5 35.0 11.5 36.0 88-89 29.394173184101394 34.0 29.0 35.0 7.0 36.0 90-91 29.076685907610305 34.0 29.0 35.0 4.5 35.0 92-93 28.77585991604484 34.0 29.0 35.0 2.0 35.0 94-95 28.409966236485083 34.0 29.0 35.0 2.0 35.0 96-97 27.9895141409717 33.0 28.5 35.0 2.0 35.0 98-99 27.502923456033777 33.0 27.0 35.0 2.0 35.0 100-101 26.375890548069354 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3664110472671318 1101 2 0.3977125310833678 1101 3 0.3745093322157125 1101 4 0.24448088050139205 1101 5 0.2773743819528107 1101 6 0.29349520645713056 1101 7 0.3120565979951948 1101 8 0.43903709257766366 1101 9 0.5132978901617804 1101 10-11 0.5495590135534414 1101 12-13 0.43068068453573005 1101 14-15 0.5060860558874154 1101 16-17 0.634211812322917 1101 18-19 0.5522007731307568 1101 20-21 0.5506787085418097 1101 22-23 0.5178246870828787 1101 24-25 0.5445954800445421 1101 26-27 0.5001847726941904 1101 28-29 0.5667447380413293 1101 30-31 0.5497755535394973 1101 32-33 0.6728500594921556 1101 34-35 0.6846452809931307 1101 36-37 0.8001664998932512 1101 38-39 0.7406361426000387 1101 40-41 0.8320084634645823 1101 42-43 0.7007304631630262 1101 44-45 0.8326191471597042 1101 46-47 0.7450282793624226 1101 48-49 0.857321040746065 1101 50-51 0.9174258970204789 1101 52-53 0.7518461021111946 1101 54-55 0.8640420098731809 1101 56-57 0.8400155840296009 1101 58-59 0.7886812641602532 1101 60-61 0.8451099262884085 1101 62-63 0.7582281391309706 1101 64-65 0.8959127251538703 1101 66-67 0.7696901422213429 1101 68-69 0.7137486847977144 1101 70-71 0.752277555748762 1101 72-73 0.7304009317772007 1101 74-75 0.6834265449911641 1101 76-77 0.7291527771914517 1101 78-79 0.8040555360170316 1101 80-81 0.6934315437416707 1101 82-83 0.7529753895399764 1101 84-85 0.6790931728233218 1101 86-87 0.8125789511998889 1101 88-89 0.721723719078252 1101 90-91 0.6322717675865555 1101 92-93 0.5713117294489187 1101 94-95 0.7407643634163321 1101 96-97 0.9103324913758541 1101 98-99 0.8009891086721197 1101 100-101 1.0787328319240643 1102 1 0.22814112884871918 1102 2 0.38877773139822125 1102 3 0.3131052784326229 1102 4 0.1113492989740692 1102 5 0.20297561295890176 1102 6 0.308226167962772 1102 7 0.3092933701868503 1102 8 0.3992964785351063 1102 9 0.5873353698430392 1102 10-11 0.378918803243117 1102 12-13 0.3801888388617414 1102 14-15 0.4813180697919037 1102 16-17 0.5397304208406268 1102 18-19 0.47901487515434127 1102 20-21 0.4885291803561955 1102 22-23 0.5480420698796991 1102 24-25 0.5914549661382367 1102 26-27 0.604513668214004 1102 28-29 0.5479874482074152 1102 30-31 0.5351459588008112 1102 32-33 0.6813926050910695 1102 34-35 0.6306909281125215 1102 36-37 0.6707811516575575 1102 38-39 0.6650613173845841 1102 40-41 0.6273228376150612 1102 42-43 0.5990969150195582 1102 44-45 0.6626312143437758 1102 46-47 0.5350157038315686 1102 48-49 0.5828464635312116 1102 50-51 0.4613372744264055 1102 52-53 0.3622276526737451 1102 54-55 0.43117575711913503 1102 56-57 0.21673392723871387 1102 58-59 0.23938302477492357 1102 60-61 0.06850755472968473 1102 62-63 -0.07134376430089162 1102 64-65 0.21232923948461035 1102 66-67 0.13247564852867555 1102 68-69 0.22938458424672348 1102 70-71 0.25626495116952697 1102 72-73 0.14223443771981437 1102 74-75 0.023181017534991355 1102 76-77 0.11069305177132804 1102 78-79 0.16099989584908414 1102 80-81 0.3066415801207434 1102 82-83 0.26439765908238044 1102 84-85 0.4245561778870197 1102 86-87 0.32445015952806244 1102 88-89 0.4645188113532761 1102 90-91 0.2815490074088274 1102 92-93 0.43037611721583247 1102 94-95 0.47415483869232133 1102 96-97 0.4939767164153217 1102 98-99 0.44527315267558976 1102 100-101 0.7181130442435659 1103 1 0.313878912776147 1103 2 0.32911991497983806 1103 3 0.3743185302052048 1103 4 0.29262032870601473 1103 5 0.3039103987094691 1103 6 0.27577444449283917 1103 7 0.28483551116271144 1103 8 0.27974870937130447 1103 9 0.4115662962966766 1103 10-11 0.5308231647025465 1103 12-13 0.43276446458805395 1103 14-15 0.5112334701264487 1103 16-17 0.5044833044027115 1103 18-19 0.5150096250518459 1103 20-21 0.4126403187734837 1103 22-23 0.3088302601168422 1103 24-25 0.37313796670556343 1103 26-27 0.44594026587397906 1103 28-29 0.3601117131005722 1103 30-31 0.3774895152797555 1103 32-33 0.4982071621121449 1103 34-35 0.6198333713941153 1103 36-37 0.6061113655827697 1103 38-39 0.4975325752687354 1103 40-41 0.5645191877490063 1103 42-43 0.540636572421306 1103 44-45 0.6186977882778777 1103 46-47 0.7024691337677638 1103 48-49 0.5457888057054419 1103 50-51 0.5046449284408254 1103 52-53 0.5633307880793481 1103 54-55 0.5478335103200109 1103 56-57 0.4807296438966091 1103 58-59 0.5092743333428444 1103 60-61 0.3812316745274771 1103 62-63 0.3479006421181836 1103 64-65 0.2751844187367496 1103 66-67 0.329812158592766 1103 68-69 0.13283853699783066 1103 70-71 0.3127471548303262 1103 72-73 0.24380028058710224 1103 74-75 0.382405885474423 1103 76-77 0.42050276299739764 1103 78-79 0.4839386238517811 1103 80-81 0.5252800538101603 1103 82-83 0.4505210901548651 1103 84-85 0.5146998767781525 1103 86-87 0.40090604762940885 1103 88-89 0.37798471038454196 1103 90-91 0.5152723785568014 1103 92-93 0.38903796308991545 1103 94-95 0.4480922509170462 1103 96-97 0.4661981086112732 1103 98-99 0.3827310119029974 1103 100-101 0.600439033978752 1104 1 0.3337897881547143 1104 2 0.4431279454586061 1104 3 0.35509068852726244 1104 4 0.20366284111016597 1104 5 0.3127477513095087 1104 6 0.3327473486748076 1104 7 0.30163366987139284 1104 8 0.4666593108920267 1104 9 0.6309248245523662 1104 10-11 0.47931805403175787 1104 12-13 0.47175950695547186 1104 14-15 0.4919717989491872 1104 16-17 0.6162929208406212 1104 18-19 0.4795042566324881 1104 20-21 0.533207244799641 1104 22-23 0.566788549149642 1104 24-25 0.45633990867991514 1104 26-27 0.5532194478444623 1104 28-29 0.5903826712808709 1104 30-31 0.6620457447404178 1104 32-33 0.6181257900843136 1104 34-35 0.5683014790363643 1104 36-37 0.6552300869887873 1104 38-39 0.6255826670916633 1104 40-41 0.5357937141360196 1104 42-43 0.6729223994720144 1104 44-45 0.802119722680871 1104 46-47 0.5519511195593694 1104 48-49 0.5521083776817264 1104 50-51 0.5478831565805251 1104 52-53 0.5570359841293495 1104 54-55 0.7243152625269786 1104 56-57 0.8138659405329918 1104 58-59 0.8206710486595554 1104 60-61 0.6276600445899803 1104 62-63 0.4636424344372827 1104 64-65 0.45008625277888825 1104 66-67 0.5101774567756294 1104 68-69 0.4134011739267649 1104 70-71 0.5240904074561428 1104 72-73 0.5881940479045831 1104 74-75 0.7565666923885317 1104 76-77 0.785958514817743 1104 78-79 0.9677949048621493 1104 80-81 0.8211167096836078 1104 82-83 0.8916636009480854 1104 84-85 0.9212480999239716 1104 86-87 1.059705342042708 1104 88-89 1.0877929494785583 1104 90-91 1.0527635184498365 1104 92-93 0.9021574657962752 1104 94-95 1.0687744590167938 1104 96-97 1.1445991814896779 1104 98-99 1.268995127664322 1104 100-101 1.3722534227924612 1105 1 0.4112874500024084 1105 2 0.43202826091102153 1105 3 0.4413492881721801 1105 4 0.262736853538037 1105 5 0.2774846497306598 1105 6 0.33710899346588263 1105 7 0.3899823398986584 1105 8 0.3325569632285905 1105 9 0.42291486957170576 1105 10-11 0.5046962204447922 1105 12-13 0.5002408770334625 1105 14-15 0.5090670608222467 1105 16-17 0.5305997379416283 1105 18-19 0.5036015911388603 1105 20-21 0.48394653685467404 1105 22-23 0.4873025661745629 1105 24-25 0.4976464404470917 1105 26-27 0.5015278540964445 1105 28-29 0.629145273525495 1105 30-31 0.626319371967611 1105 32-33 0.6168458498458946 1105 34-35 0.6483165185075421 1105 36-37 0.7241941864434907 1105 38-39 0.9002991165875756 1105 40-41 0.6499623226579914 1105 42-43 0.753030271515641 1105 44-45 0.7242410212668773 1105 46-47 0.794158038112414 1105 48-49 0.8146112508349859 1105 50-51 0.7808125641957702 1105 52-53 0.7830549627927255 1105 54-55 0.8767087973377059 1105 56-57 0.7860927531954758 1105 58-59 0.8048807928102164 1105 60-61 0.7963107721912692 1105 62-63 0.583382186196836 1105 64-65 0.6406697406680522 1105 66-67 0.5663790094672834 1105 68-69 0.6331679729566204 1105 70-71 0.6094572397749545 1105 72-73 0.7150639753029715 1105 74-75 0.7749226740954605 1105 76-77 0.7128154634893562 1105 78-79 0.8890346407698324 1105 80-81 0.9243666423131174 1105 82-83 0.9256022779501798 1105 84-85 0.9185970711369578 1105 86-87 0.9327298014936005 1105 88-89 1.0146225536571158 1105 90-91 0.9341011000870196 1105 92-93 1.072184235658682 1105 94-95 1.049730902013433 1105 96-97 1.1279971112341478 1105 98-99 1.2314030876858801 1105 100-101 1.2296602137378692 1106 1 0.35108198158075865 1106 2 0.2615026294029832 1106 3 0.3157378124210659 1106 4 0.18966947827830438 1106 5 0.25083640491254044 1106 6 0.27609437775192447 1106 7 0.38941416923928074 1106 8 0.3153437293496708 1106 9 0.42691091810483073 1106 10-11 0.3480226210355468 1106 12-13 0.29391303097570187 1106 14-15 0.5506277724005102 1106 16-17 0.3680173993488438 1106 18-19 0.31113549873109747 1106 20-21 0.3954030412598186 1106 22-23 0.29132434244926486 1106 24-25 0.3334594567203695 1106 26-27 0.33401511788239446 1106 28-29 0.331469902633593 1106 30-31 0.3895702390514231 1106 32-33 0.5285180941298222 1106 34-35 0.5154075947127197 1106 36-37 0.3786991925513732 1106 38-39 0.4144772625404656 1106 40-41 0.4779120769678755 1106 42-43 0.5377849152747984 1106 44-45 0.5134977252093123 1106 46-47 0.5215169381434421 1106 48-49 0.5316141456968992 1106 50-51 0.49351961359696617 1106 52-53 0.4201542679473036 1106 54-55 0.597914741035197 1106 56-57 0.5710660827580227 1106 58-59 0.4566816365230224 1106 60-61 0.5408119093181725 1106 62-63 0.38981579094802754 1106 64-65 0.3718763128294569 1106 66-67 0.3502197292155813 1106 68-69 0.3843062782251181 1106 70-71 0.38025546750670713 1106 72-73 0.3693294777402336 1106 74-75 0.49144145534680916 1106 76-77 0.5895042638695784 1106 78-79 0.4878446836232122 1106 80-81 0.5067342033624946 1106 82-83 0.580645111693979 1106 84-85 0.6115491429075384 1106 86-87 0.712921208035123 1106 88-89 0.7203130980247039 1106 90-91 0.7155637712943381 1106 92-93 0.6231889923449465 1106 94-95 0.8897546265262726 1106 96-97 0.8270501928676843 1106 98-99 0.8705813318362559 1106 100-101 1.1522312988986556 1107 1 0.15720438682112814 1107 2 0.14914578687522706 1107 3 0.12091934330489096 1107 4 0.06607202208770957 1107 5 0.09417644068486197 1107 6 0.08303120726447588 1107 7 0.1907552415515852 1107 8 0.13200123861423663 1107 9 0.1160367953644652 1107 10-11 0.2699046230384923 1107 12-13 0.20468720164629417 1107 14-15 0.17027293281092426 1107 16-17 0.10345313581829885 1107 18-19 0.22920889823153345 1107 20-21 0.25994519063847576 1107 22-23 0.12824776708384178 1107 24-25 0.13933188436715227 1107 26-27 0.13555200272903534 1107 28-29 0.2168703733630224 1107 30-31 0.2897178290959559 1107 32-33 0.3538608147744995 1107 34-35 0.21340835847902184 1107 36-37 0.29702325157959564 1107 38-39 0.2369203015509953 1107 40-41 0.24362754284583588 1107 42-43 0.21810663234614225 1107 44-45 0.25814444432646155 1107 46-47 0.3235006847289057 1107 48-49 0.2821320260116025 1107 50-51 0.2209850979845669 1107 52-53 0.06720918897932648 1107 54-55 0.12717478397755855 1107 56-57 0.2326999776154608 1107 58-59 0.30980362870609923 1107 60-61 0.30538835024513133 1107 62-63 -0.061694303626502744 1107 64-65 -0.024173729450460257 1107 66-67 0.052611662128846604 1107 68-69 0.1193481489849475 1107 70-71 0.09851630149275792 1107 72-73 0.11231003677040619 1107 74-75 0.27045541746243984 1107 76-77 0.3643031906999852 1107 78-79 0.46248199204841356 1107 80-81 0.29111586439833204 1107 82-83 0.5219816247890599 1107 84-85 0.6105697879783207 1107 86-87 0.5353395025761429 1107 88-89 0.7078031125596418 1107 90-91 0.6123360793103743 1107 92-93 0.44460503845149546 1107 94-95 0.45271040211926206 1107 96-97 0.5541768533774594 1107 98-99 0.6381635438749278 1107 100-101 0.8735358974732179 1108 1 0.04842610823526883 1108 2 0.14293244263264882 1108 3 0.003008776294429083 1108 4 -0.032453007002615664 1108 5 -0.05226576944667727 1108 6 0.033349083229474275 1108 7 0.01587329989802555 1108 8 -0.007422049141759146 1108 9 0.21361988074674088 1108 10-11 0.1075316697986679 1108 12-13 0.12739285084555263 1108 14-15 0.09488079167315533 1108 16-17 0.05908986994086263 1108 18-19 0.16705653282946287 1108 20-21 0.04948595102974451 1108 22-23 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-1.5950433640644128 2316 90-91 -1.4886877210601455 2316 92-93 -1.5133293252233067 2316 94-95 -2.0714895698729556 2316 96-97 -1.8527005435995463 2316 98-99 -2.00343496903675 2316 100-101 -2.413507887133264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 12.0 4 31.0 5 64.0 6 129.0 7 370.0 8 836.0 9 1452.0 10 2594.0 11 4126.0 12 5913.0 13 7699.0 14 9343.0 15 12255.0 16 15119.0 17 17871.0 18 15402.0 19 11901.0 20 11279.0 21 11780.0 22 12842.0 23 14691.0 24 18160.0 25 23004.0 26 29286.0 27 37261.0 28 46770.0 29 58268.0 30 70922.0 31 85928.0 32 103406.0 33 125967.0 34 156053.0 35 195996.0 36 260990.0 37 347681.0 38 289457.0 39 45272.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.53028104261126 21.500942662234245 14.809723688992193 23.1590526061623 2 15.509793926326648 21.533808619045413 39.683267520164684 23.273129934463256 3 18.263064574741293 26.29221430121872 30.860029120459682 24.584692003580304 4 12.830581773106095 17.726956690837792 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21.539907600958866 27.144114554714715 30.15290009728654 21.163077747039882 26-27 21.30343577682828 27.42943661727939 29.89498751152084 21.37214009437149 28-29 21.331159820051052 27.417314062426072 29.984825404351028 21.26670071317185 30-31 21.590557929655688 27.161854550926783 29.836686680326718 21.410900839090807 32-33 21.588170077095988 27.456710497910073 29.481184602268364 21.47393482272557 34-35 21.746394992431178 27.29697730134346 29.750782615567818 21.205845090657544 36-37 21.797638044732427 27.03171296753586 29.859323109071234 21.31132587866048 38-39 21.62021564926594 27.07205307749321 29.97530162045466 21.332429652786196 40-41 21.513074089637872 27.118129831918324 30.00768985190755 21.361106226536258 42-43 21.59105543836918 27.298484425703297 29.415790695813875 21.694669440113643 44-45 21.417609718667332 27.61006988804352 29.506173923520713 21.466146469768432 46-47 21.751254852882386 27.578567485950206 29.05163391247994 21.618543748687465 48-49 21.913111773192533 27.61610443270693 28.728726433675256 21.74205736042528 50-51 21.94583005697804 27.849378392524386 28.36009885511236 21.844692695385216 52-53 21.872494298843165 27.817103958824706 28.469379782501473 21.841021959830652 54-55 21.68658534949269 27.96163514111794 28.58052705334808 21.77125245604128 56-57 21.835224298796447 27.927435511321647 28.439725445375657 21.797614744506248 58-59 21.628878150635312 27.80020831453055 28.731476715850686 21.839436818983454 60-61 21.589715364588546 28.22509556137147 28.64001285384079 21.545176220199185 62-63 21.787455745819535 27.975218147868734 28.570073539801506 21.667252566510225 64-65 21.265889629797144 27.90824689969623 28.648945988399454 22.176917482107168 66-67 21.459259998200462 27.882025537807927 28.57638848138455 22.082325982607063 68-69 21.43725958899605 27.92724731799544 28.66010893049636 21.975384162512153 70-71 21.490465098996346 27.621877762393122 28.708133854325563 22.179523284284965 72-73 21.694877246603102 27.9642116638689 28.450124775999235 21.890786313528768 74-75 21.586406201356258 28.294556107410024 28.256405352746523 21.862632338487188 76-77 21.52870719143765 28.106935497904484 28.376073055253855 21.988284255404015 78-79 21.309186765500705 28.209138766343617 28.45806122429069 22.023613243864986 80-81 21.525007304375652 28.346611281858173 28.21625439420996 21.912127019556216 82-83 21.68198593073065 28.06337317122424 28.468954833372518 21.785686064672593 84-85 21.589553308990304 28.27262696925384 28.037448137738753 22.100371584017108 86-87 21.400468845572647 28.50026973741331 28.17965779931341 21.919603617700627 88-89 21.483825494844254 28.16600329733579 28.023891051342837 22.326280156477118 90-91 21.39533998872692 28.04092064624846 28.427137049872524 22.1366023151521 92-93 21.518219117266817 28.119032867102163 28.204042404757608 22.158705610873415 94-95 21.078829369894287 27.93469075175876 28.449301677851118 22.53717820049583 96-97 21.282579763396818 27.639701942524454 28.458499084087613 22.619219209991115 98-99 21.01930012652782 28.10402889165726 28.17763354928585 22.699037432529064 100-101 21.04000600933984 27.8616310479957 28.238873209758154 22.859489732906308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 96.0 1 99.0 2 151.5 3 235.5 4 220.5 5 184.5 6 334.0 7 749.0 8 1303.5 9 1634.5 10 1501.5 11 1278.5 12 1321.0 13 1521.0 14 1783.5 15 2094.0 16 2422.0 17 2724.5 18 3054.0 19 3386.5 20 3742.5 21 4199.5 22 4670.0 23 5408.0 24 6521.0 25 7967.5 26 9679.0 27 11401.5 28 14047.0 29 18646.0 30 24305.5 31 30218.0 32 38427.5 33 44064.5 34 46609.5 35 50573.5 36 56416.0 37 64765.5 38 69806.0 39 70212.0 40 69879.0 41 70356.0 42 71996.5 43 75919.5 44 80037.0 45 85805.5 46 90559.0 47 92148.0 48 93306.5 49 91045.5 50 85393.5 51 79126.5 52 75032.5 53 71465.0 54 65929.5 55 56815.5 56 46388.5 57 36998.5 58 29015.5 59 22795.0 60 17213.0 61 12643.0 62 9240.5 63 5953.5 64 3261.5 65 1806.5 66 951.0 67 510.5 68 279.0 69 158.5 70 103.5 71 70.5 72 57.5 73 47.5 74 34.0 75 25.0 76 18.5 77 10.5 78 6.5 79 5.5 80 4.0 81 1.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0047313795824874575 2 0.007170235037377899 3 0.001804753036618927 4 0.1699882252058638 5 0.0017559759275211182 6 4.8777109097808836E-4 7 0.0 8 8.292108546627501E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.1705120913575743E-4 24-25 6.097138637226105E-4 26-27 0.002560798227634964 28-29 2.4388554548904418E-4 30-31 0.006121527191775009 32-33 0.020705882812019853 34-35 0.013901476092875517 36-37 9.023765183094635E-4 38-39 3.6582831823356624E-4 40-41 0.001829141591167831 42-43 0.011023626656104797 44-45 0.0072677892555735165 46-47 0.009779810374110671 48-49 0.010218804355990952 50-51 0.19096238211792158 52-53 0.11277267623413403 54-55 0.017047599629684187 56-57 0.006389801291812957 58-59 0.017413427947917753 60-61 0.12218665829001113 62-63 0.2368128646698619 64-65 0.2428856147525391 66-67 0.2523727624720629 68-69 0.24761699433502654 70-71 0.23481300319685172 72-73 0.15969625518622613 74-75 0.27420051879333235 76-77 0.21208287035727283 78-79 0.005170373564367736 80-81 4.877710909780884E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.755421819561768E-5 90-91 0.006121527191775009 92-93 0.018145084584384887 94-95 0.0024144669003415375 96-97 2.438855454890442E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2050142.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.107567040622705 #Duplication Level Percentage of deduplicated Percentage of total 1 64.79590217672941 24.044182839806176 2 16.140243309404415 11.978503213113731 3 6.352581195774191 7.0718649780957 4 3.4814693719113903 5.167554324723062 5 2.084153429013352 3.8668931545028333 6 1.3937732094031483 3.1031719684410723 7 0.9749311199846183 2.532412532637302 8 0.7222058731868791 2.1439442285130865 9 0.5399141027718325 1.8031408888305092 >10 3.172117592868398 21.568155463692147 >50 0.22038560748740255 5.623437055686893 >100 0.11320942893248165 7.608946636478672 >500 0.006868207125922472 1.7457098021405653 >1k 0.0019812135940160975 1.1647214256294118 >5k 2.641618125354797E-4 0.5773614877089073 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6420 0.31314904040793273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5360 0.26144530476425537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.16257410462299685 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 2287 0.11155324850668881 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.8777109097808835E-5 0.0 22-23 0.0 0.0 0.0 9.755421819561767E-5 2.4388554548904417E-5 24-25 0.0 0.0 0.0 1.463313272934265E-4 4.8777109097808835E-5 26-27 0.0 0.0 0.0 2.1949699094013975E-4 4.8777109097808835E-5 28-29 0.0 0.0 0.0 6.828795273693237E-4 4.8777109097808835E-5 30-31 0.0 0.0 0.0 0.003219289200455383 7.316566364671325E-5 32-33 0.0 0.0 0.0 0.010633409783322327 9.755421819561767E-5 34-35 0.0 0.0 0.0 0.0204376087119819 9.755421819561767E-5 36-37 0.0 0.0 0.0 0.037387654123470476 9.755421819561767E-5 38-39 0.0 0.0 0.0 0.06309319061801573 1.9510843639123534E-4 40-41 0.0 0.0 0.0 0.09255456451309227 1.9510843639123534E-4 42-43 0.0 0.0 0.0 0.12499134206313514 1.9510843639123534E-4 44-45 0.0 0.0 0.0 0.1660860564780391 1.9510843639123534E-4 46-47 0.0 0.0 0.0 0.2164971987306245 1.9510843639123534E-4 48-49 0.0 0.0 0.0 0.2698593560836274 1.9510843639123534E-4 50-51 0.0 0.0 0.0 0.32597742010065645 1.9510843639123534E-4 52-53 0.0 0.0 0.0 0.38470505945441824 2.4388554548904418E-4 54-55 0.0 0.0 0.0 0.44960300310905293 2.4388554548904418E-4 56-57 0.0 0.0 0.0 0.5287926397293456 2.4388554548904418E-4 58-59 0.0 0.0 0.0 0.6160061107962278 2.4388554548904418E-4 60-61 0.0 0.0 0.0 0.7120238500552645 2.4388554548904418E-4 62-63 0.0 0.0 0.0 0.8153581556789725 2.4388554548904418E-4 64-65 0.0 0.0 0.0 0.9192046209482074 2.4388554548904418E-4 66-67 0.0 0.0 0.0 1.0404157370562626 2.4388554548904418E-4 68-69 0.0 0.0 0.0 1.182162016094495 2.4388554548904418E-4 70-71 0.0 0.0 0.0 1.341614385735232 2.4388554548904418E-4 72-73 0.0 0.0 0.0 1.5075541108859776 2.4388554548904418E-4 74-75 0.0 0.0 0.0 1.6762497427007497 2.4388554548904418E-4 76-77 0.0 0.0 0.0 1.8663829139640082 2.4388554548904418E-4 78-79 0.0 0.0 0.0 2.082367953049106 2.4388554548904418E-4 80-81 0.0 0.0 0.0 2.324229248510591 2.682741000379486E-4 82-83 0.0 0.0 0.0 2.58269914961988 2.92662654586853E-4 84-85 0.0 0.0 0.0 2.8469979152663574 2.92662654586853E-4 86-87 0.0 0.0 0.0 3.131514792633876 2.92662654586853E-4 88-89 0.0 0.0 0.0 3.448127007787753 2.92662654586853E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2490 0.0 61.266987 1 GTATCAA 5480 0.0 47.091236 1 CAACGCA 7325 0.0 34.39097 5 GTGGTAT 1080 0.0 34.32351 1 TATCAAC 7435 0.0 34.131023 2 ATCAACG 7470 0.0 33.651382 3 TCAACGC 7585 0.0 33.015068 4 AACGCAG 7910 0.0 32.32733 6 ACGCAGA 9185 0.0 27.220055 7 CGCAGAG 9355 0.0 26.725409 8 TGGTATC 1405 0.0 25.367283 2 GTACATG 6115 0.0 25.180792 1 ACATGGG 6105 0.0 24.440596 3 TACATGG 6410 0.0 24.094301 2 GCAGAGT 10485 0.0 23.437908 9 GGGCTAT 1205 0.0 22.441162 6 GTACTAG 490 0.0 22.307587 1 CATGGGT 1375 0.0 22.11742 4 TCTAACG 155 3.8948034E-5 21.461266 2 AGAGTAC 9910 0.0 21.351027 10-11 >>END_MODULE