FastQCFastQC Report
Sat 18 Jun 2016
SRR1930974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122842
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN915494.312567774709564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.11098329503561734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.057.934431
GTATCAA34050.047.1647761
ATCAACG42250.037.8550763
TCAACGC44600.036.0771874
CAACGCA44350.035.9354065
TATCAAC45650.034.7292442
AACGCAG49950.033.3548746
ACGCAGA56300.028.5229917
CGCAGAG57250.028.118118
GCAGAGT64050.024.5182279
ACATGGG40200.024.3967693
GTACATG42550.024.1602671
TACATGG41250.023.9280722
AGAGTAC55250.022.244910-11
GTACTAG6050.021.9797741
GAGTACA29700.021.5257761
GCGGTCG2350.021.00874522-23
AGTACTT45200.020.97677212-13
GAGTACT41550.020.56643312-13
CATGGGT10000.020.1130314
Produced by FastQC (version 0.11.5)