##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930974_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2122842 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.968187458133954 31.0 10.0 31.0 2.0 33.0 2 21.531369268179166 30.0 2.0 31.0 2.0 34.0 3 22.34681808632013 30.0 10.0 31.0 2.0 34.0 4 23.8689233584035 33.0 2.0 37.0 2.0 37.0 5 23.18693666320904 33.0 2.0 37.0 2.0 37.0 6 24.306106624986693 33.0 2.0 37.0 2.0 37.0 7 24.665386778667465 33.0 2.0 37.0 2.0 37.0 8 25.22021987505429 35.0 10.0 37.0 2.0 37.0 9 26.857838689831837 35.0 17.0 39.0 2.0 39.0 10-11 27.40141164533206 35.0 17.0 39.0 2.0 39.0 12-13 27.386698350607347 35.0 14.0 39.0 2.0 39.0 14-15 27.477243478318215 36.0 10.0 39.5 2.0 41.0 16-17 26.211368297781938 35.5 2.0 39.0 2.0 41.0 18-19 25.017983203648694 34.0 2.0 39.0 2.0 41.0 20-21 24.659220045580405 34.0 2.0 39.0 2.0 40.0 22-23 24.30887838096288 33.0 2.0 39.0 2.0 40.0 24-25 24.193093739430445 33.0 2.0 39.0 2.0 40.0 26-27 23.934570966656963 32.5 2.0 39.0 2.0 40.0 28-29 23.81245542532134 32.0 2.0 38.0 2.0 40.0 30-31 23.946408635216375 32.0 2.0 38.0 2.0 40.0 32-33 23.885346860482315 32.5 2.0 38.0 2.0 40.0 34-35 23.609393445202233 32.0 2.0 38.0 2.0 40.0 36-37 23.096156002189517 31.0 2.0 38.0 2.0 40.0 38-39 23.09642710102777 31.0 2.0 38.0 2.0 40.0 40-41 23.069871427077473 31.0 2.0 38.0 2.0 40.0 42-43 22.992517342317516 31.0 2.0 38.0 2.0 40.0 44-45 22.63194363970564 30.0 2.0 38.0 2.0 40.0 46-47 22.315690946382254 30.0 2.0 38.0 2.0 40.0 48-49 22.179140275159433 30.0 2.0 38.0 2.0 40.0 50-51 19.370983568254253 19.5 2.0 36.0 2.0 39.0 52-53 21.657068919872508 28.5 2.0 37.0 2.0 39.0 54-55 22.597556954309365 31.0 2.0 38.0 2.0 40.0 56-57 22.64581325411877 31.0 2.0 38.0 2.0 40.0 58-59 22.399118964105668 31.0 2.0 38.0 2.0 40.0 60-61 22.151520226187348 30.0 2.0 38.0 2.0 40.0 62-63 21.74018909556152 29.5 2.0 37.5 2.0 40.0 64-65 21.463892037184113 29.0 2.0 37.0 2.0 40.0 66-67 21.17157141228598 28.0 2.0 36.5 2.0 39.0 68-69 20.765574168967824 28.0 2.0 36.0 2.0 39.0 70-71 20.47766202100769 28.0 2.0 35.5 2.0 39.0 72-73 20.10358330954447 26.5 2.0 35.0 2.0 38.5 74-75 19.687614057004716 26.0 2.0 35.0 2.0 38.0 76-77 19.334539734940236 26.0 2.0 34.0 2.0 37.0 78-79 19.01770621647772 25.5 2.0 34.0 2.0 36.5 80-81 18.643163739929776 24.5 2.0 34.0 2.0 36.0 82-83 18.224951268158442 22.5 2.0 34.0 2.0 36.0 84-85 17.89270256571144 21.5 2.0 34.0 2.0 35.0 86-87 17.45416309833704 20.0 2.0 33.0 2.0 35.0 88-89 17.098971096294495 18.0 2.0 33.0 2.0 35.0 90-91 16.663889964491 13.5 2.0 32.5 2.0 35.0 92-93 16.160248855072588 6.5 2.0 32.0 2.0 35.0 94-95 15.63202042356426 2.0 2.0 32.0 2.0 34.0 96-97 15.021163609915387 2.0 2.0 32.0 2.0 34.0 98-99 14.332178042454409 2.0 2.0 31.0 2.0 34.0 100-101 13.14066355385846 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.342923404546987 1101 2 10.075559639007182 1101 3 9.310675494535797 1101 4 11.454847782502739 1101 5 12.23862104337405 1101 6 11.226871208341922 1101 7 10.797570300416453 1101 8 10.262054710246556 1101 9 10.085573569523643 1101 10-11 9.470797615975922 1101 12-13 9.382580623137056 1101 14-15 10.137478572178807 1101 16-17 11.395715102055277 1101 18-19 12.498129399840494 1101 20-21 12.709510141678763 1101 22-23 12.857476474770312 1101 24-25 12.980464868464214 1101 26-27 12.968969160260105 1101 28-29 12.950570805351699 1101 30-31 12.46004084817709 1101 32-33 12.37762359973198 1101 34-35 12.445666187087895 1101 36-37 12.86756601670314 1101 38-39 12.71346690426844 1101 40-41 12.292121497017142 1101 42-43 12.16842380449765 1101 44-45 12.297894215608341 1101 46-47 11.997078938696038 1101 48-49 11.95744514498372 1101 50-51 14.054089100392027 1101 52-53 12.744545247464306 1101 54-55 12.485262336071823 1101 56-57 12.295195401398548 1101 58-59 12.242960054922083 1101 60-61 12.005505651896858 1101 62-63 12.248290713585416 1101 64-65 12.060817279408631 1101 66-67 11.861264666366022 1101 68-69 11.98407116557863 1101 70-71 11.807709702470262 1101 72-73 11.632133727058324 1101 74-75 11.544675864759046 1101 76-77 11.354867328314032 1101 78-79 11.039825474043706 1101 80-81 10.932449825167446 1101 82-83 10.88486770441111 1101 84-85 10.343731140045303 1101 86-87 10.43114844015884 1101 88-89 10.404429848059149 1101 90-91 10.123191775827017 1101 92-93 10.08111571623941 1101 94-95 9.988246193850513 1101 96-97 10.115205498294827 1101 98-99 9.995362684920554 1101 100-101 9.45774785792593 1102 1 8.249955577119248 1102 2 9.73819280879587 1102 3 9.013914181323997 1102 4 11.07554082689964 1102 5 12.017625594889378 1102 6 11.050553511789051 1102 7 10.639017298619045 1102 8 9.967690417171749 1102 9 9.726586371832429 1102 10-11 8.98009773678508 1102 12-13 9.091591898177452 1102 14-15 9.746960761843212 1102 16-17 10.975916164920335 1102 18-19 12.142144607411552 1102 20-21 12.345683674974637 1102 22-23 12.43545659019735 1102 24-25 12.372645942493254 1102 26-27 12.670940618144119 1102 28-29 12.4206294832013 1102 30-31 12.240541760066765 1102 32-33 12.227690845285299 1102 34-35 12.261205772125024 1102 36-37 12.3864379398353 1102 38-39 12.195768043599944 1102 40-41 12.11818701501808 1102 42-43 11.788613666295518 1102 44-45 11.650783672655354 1102 46-47 11.496942737345524 1102 48-49 11.225050096778535 1102 50-51 13.501014463082207 1102 52-53 12.02614277597645 1102 54-55 11.730020180991442 1102 56-57 11.583275063322478 1102 58-59 11.699975547465112 1102 60-61 11.730265491414162 1102 62-63 11.715383663692247 1102 64-65 11.52138338249842 1102 66-67 11.527805537382928 1102 68-69 11.262859463966326 1102 70-71 11.001005587644077 1102 72-73 11.045339777035775 1102 74-75 10.873603053495177 1102 76-77 10.606087113404374 1102 78-79 10.276097830588757 1102 80-81 10.033217910120818 1102 82-83 10.032943916999784 1102 84-85 9.927051646549486 1102 86-87 9.653340029096984 1102 88-89 9.601573781978622 1102 90-91 9.370980358362463 1102 92-93 9.285613595846073 1102 94-95 8.902961688598308 1102 96-97 8.947481159113487 1102 98-99 8.546055859131148 1102 100-101 8.056409458149624 1103 1 8.068052960078905 1103 2 9.87210396906541 1103 3 9.011346285952307 1103 4 11.25727666602073 1103 5 11.942981370957913 1103 6 10.938363661174417 1103 7 10.572938917842848 1103 8 10.038992419677857 1103 9 9.826195998431611 1103 10-11 9.194069567065736 1103 12-13 9.051300190270606 1103 14-15 9.939484467123606 1103 16-17 10.89313071522367 1103 18-19 12.063684682368738 1103 20-21 12.475726435540906 1103 22-23 12.719086166343178 1103 24-25 12.704500888508488 1103 26-27 12.58214672132755 1103 28-29 12.337764581796126 1103 30-31 12.082386319815466 1103 32-33 12.092524105729002 1103 34-35 12.17607301407456 1103 36-37 12.51148604496758 1103 38-39 12.035852046366923 1103 40-41 11.923125398047596 1103 42-43 11.659634872128137 1103 44-45 11.662002718289916 1103 46-47 11.68678931377443 1103 48-49 11.751891952948132 1103 50-51 13.735267881524777 1103 52-53 12.243101768395523 1103 54-55 11.730608850511498 1103 56-57 11.827554944130778 1103 58-59 11.581027253869745 1103 60-61 11.469373136667432 1103 62-63 11.582304373915814 1103 64-65 11.288877497822327 1103 66-67 11.329762714628284 1103 68-69 11.314793522480745 1103 70-71 11.12740073743528 1103 72-73 11.00566197743822 1103 74-75 10.83865964693705 1103 76-77 10.68558051638869 1103 78-79 10.32896047463662 1103 80-81 10.148323143757302 1103 82-83 9.920975158178827 1103 84-85 9.654275658370125 1103 86-87 9.406281357594164 1103 88-89 9.322382901217566 1103 90-91 9.217495305581085 1103 92-93 9.038497783724424 1103 94-95 8.966395044262923 1103 96-97 8.81202568780838 1103 98-99 8.358427469410323 1103 100-101 7.643577068343184 1104 1 8.096117036997043 1104 2 9.762975847919027 1104 3 8.99265672533205 1104 4 11.278142664632 1104 5 12.11502513130397 1104 6 11.014299133687143 1104 7 10.46776174452615 1104 8 9.947719282223947 1104 9 9.54548236623388 1104 10-11 8.987769161120266 1104 12-13 8.75100698555428 1104 14-15 9.530065405922223 1104 16-17 10.811203342715245 1104 18-19 11.91821819955318 1104 20-21 12.20714685868991 1104 22-23 12.494394051972666 1104 24-25 12.318943283598546 1104 26-27 12.358241429070052 1104 28-29 12.165560424350577 1104 30-31 12.060941847932508 1104 32-33 12.087696537041825 1104 34-35 12.157521007013543 1104 36-37 12.223979216521382 1104 38-39 12.043287618721674 1104 40-41 11.679788031647846 1104 42-43 11.271343427152118 1104 44-45 11.454786502999784 1104 46-47 11.438663173320531 1104 48-49 11.415707411014285 1104 50-51 13.557559415511825 1104 52-53 12.028576211003294 1104 54-55 11.644965562632873 1104 56-57 11.521672752206445 1104 58-59 11.644734441432146 1104 60-61 11.582938615509395 1104 62-63 11.744179411085799 1104 64-65 11.374428820758702 1104 66-67 11.170611868097883 1104 68-69 10.962229965253144 1104 70-71 10.852400058036885 1104 72-73 10.632316607922295 1104 74-75 10.6627368171423 1104 76-77 10.333671938599938 1104 78-79 10.104880015749284 1104 80-81 9.998910439361772 1104 82-83 9.794621826113705 1104 84-85 9.587704461947581 1104 86-87 9.56490690116211 1104 88-89 9.377661314741573 1104 90-91 9.119336908472924 1104 92-93 8.769041881406554 1104 94-95 8.556253457637267 1104 96-97 8.381287006487184 1104 98-99 8.099637334196256 1104 100-101 7.521161194603373 1105 1 7.933099325028561 1105 2 9.432889705596207 1105 3 8.785907634961141 1105 4 11.165381417866357 1105 5 11.271373225606698 1105 6 10.658966532515056 1105 7 10.133107848735214 1105 8 9.790295878283107 1105 9 9.597483608043074 1105 10-11 8.777610683989966 1105 12-13 8.502343269758224 1105 14-15 9.343921599252873 1105 16-17 10.641508401122088 1105 18-19 11.456619375995892 1105 20-21 11.9864469747193 1105 22-23 12.179155083676374 1105 24-25 12.123169096310054 1105 26-27 12.263755050333284 1105 28-29 11.98565052296495 1105 30-31 11.61271598357531 1105 32-33 11.566532400660964 1105 34-35 11.761695386007773 1105 36-37 11.643490266676874 1105 38-39 11.505754783717915 1105 40-41 11.159454273265297 1105 42-43 11.0569488467127 1105 44-45 10.992270486571062 1105 46-47 10.889288539104061 1105 48-49 10.765047184386031 1105 50-51 12.614108947299272 1105 52-53 11.216024938678817 1105 54-55 10.83524083226484 1105 56-57 10.827353412608435 1105 58-59 10.835248572407561 1105 60-61 10.821268470787746 1105 62-63 10.890433469753386 1105 64-65 10.86848850583943 1105 66-67 10.601670548978163 1105 68-69 10.42709913687738 1105 70-71 10.28326275506377 1105 72-73 10.157143541846445 1105 74-75 9.963851458446559 1105 76-77 9.502511450178112 1105 78-79 9.384181577087315 1105 80-81 9.144714332560785 1105 82-83 9.028355021750876 1105 84-85 8.72369393855892 1105 86-87 8.711444433033574 1105 88-89 8.356600277044645 1105 90-91 8.17140945217568 1105 92-93 8.166403383741304 1105 94-95 8.032823077287913 1105 96-97 7.870576016764872 1105 98-99 7.391390133229169 1105 100-101 7.041149331509942 1106 1 7.893106364951006 1106 2 9.32650785710237 1106 3 8.862600822323838 1106 4 10.727896742079928 1106 5 11.583031953456416 1106 6 10.596890069202711 1106 7 9.92672431776797 1106 8 9.334441617532622 1106 9 8.982835994537087 1106 10-11 8.382378681304058 1106 12-13 8.191163290662484 1106 14-15 8.94863486892065 1106 16-17 10.339904342315677 1106 18-19 11.709644283596909 1106 20-21 11.837530969652214 1106 22-23 12.069720302111524 1106 24-25 11.750624963992781 1106 26-27 11.985950145034796 1106 28-29 11.794686293672928 1106 30-31 11.404265278817444 1106 32-33 11.645108634948972 1106 34-35 11.604868353159276 1106 36-37 12.001193943788675 1106 38-39 11.62764525621127 1106 40-41 11.582436217300938 1106 42-43 11.246841869760132 1106 44-45 10.962038707899868 1106 46-47 10.782073110104538 1106 48-49 11.009869939593148 1106 50-51 13.238816712065294 1106 52-53 11.935661539545652 1106 54-55 11.081196043310339 1106 56-57 11.187595639628544 1106 58-59 11.189458848690489 1106 60-61 11.016254095092183 1106 62-63 11.198165418089733 1106 64-65 11.18701560056136 1106 66-67 10.828981780660232 1106 68-69 10.597452499773095 1106 70-71 10.356383616580896 1106 72-73 10.383344246557048 1106 74-75 10.117090569488102 1106 76-77 9.781454577094642 1106 78-79 9.580994669981965 1106 80-81 9.245246466763184 1106 82-83 9.369370925661606 1106 84-85 8.940069093077089 1106 86-87 8.979034267188958 1106 88-89 8.41920596331326 1106 90-91 8.314777075829443 1106 92-93 7.893620188966274 1106 94-95 7.983314217742118 1106 96-97 7.65344856978216 1106 98-99 7.39078160717229 1106 100-101 6.738132089560249 1107 1 7.555660982541237 1107 2 8.89962114638372 1107 3 8.673444860449202 1107 4 10.560704848918213 1107 5 11.45663104173821 1107 6 10.335342613895254 1107 7 10.090408641021156 1107 8 8.907947477401397 1107 9 6.393951139296778 1107 10-11 8.040821817937367 1107 12-13 8.136276979899971 1107 14-15 8.76615942446655 1107 16-17 9.959636383063483 1107 18-19 11.536204496028155 1107 20-21 11.783791408138228 1107 22-23 11.420579838537353 1107 24-25 11.55636728884011 1107 26-27 11.505142074556165 1107 28-29 11.628879759957595 1107 30-31 11.363947256873256 1107 32-33 11.60031070653999 1107 34-35 11.6764156209873 1107 36-37 12.080306291127858 1107 38-39 11.46466966205767 1107 40-41 11.252206236838568 1107 42-43 10.997009322988262 1107 44-45 10.660465244080843 1107 46-47 10.930343782695548 1107 48-49 10.968809101378245 1107 50-51 12.90273396432115 1107 52-53 11.340488737472246 1107 54-55 11.054325410374059 1107 56-57 10.790088661700555 1107 58-59 10.819039241295162 1107 60-61 10.876846371052839 1107 62-63 11.055143601732095 1107 64-65 10.9341407737668 1107 66-67 10.87987932428187 1107 68-69 10.579696776395036 1107 70-71 10.327174035586086 1107 72-73 10.071648597273136 1107 74-75 10.04965677103532 1107 76-77 9.869003215226936 1107 78-79 9.718173377089652 1107 80-81 9.312760370068105 1107 82-83 9.165032821828675 1107 84-85 9.187137738390415 1107 86-87 8.812311356689936 1107 88-89 8.843000967611086 1107 90-91 8.77084950652312 1107 92-93 8.26759934920609 1107 94-95 8.177994005697185 1107 96-97 7.930346614485213 1107 98-99 7.996053869929838 1107 100-101 7.399391110588056 1108 1 6.996596864543317 1108 2 8.544390824137519 1108 3 8.206998179786556 1108 4 9.970640615530975 1108 5 11.599694852031288 1108 6 9.971512055933356 1108 7 9.269735697405203 1108 8 6.530881592396899 1108 9 7.64131598596963 1108 10-11 6.406179705658399 1108 12-13 5.2438743618828205 1108 14-15 7.644277878203759 1108 16-17 9.095901982866913 1108 18-19 9.005506918801991 1108 20-21 9.240305135161535 1108 22-23 10.100917269052605 1108 24-25 9.930619626334927 1108 26-27 8.384312809987971 1108 28-29 10.08514979162938 1108 30-31 10.441994927013663 1108 32-33 10.86095881561906 1108 34-35 10.498959191936514 1108 36-37 10.219669606165379 1108 38-39 10.047322571562024 1108 40-41 10.34681715381624 1108 42-43 9.538456477263786 1108 44-45 9.11852897687363 1108 46-47 9.894044082765188 1108 48-49 10.223066307039325 1108 50-51 12.344355580357167 1108 52-53 11.075100768157764 1108 54-55 10.293045530424873 1108 56-57 10.113193392145934 1108 58-59 10.302812116079377 1108 60-61 10.363342663381626 1108 62-63 10.50925539388895 1108 64-65 10.135005734393783 1108 66-67 9.829138737265982 1108 68-69 9.918715473483147 1108 70-71 10.024033953527631 1108 72-73 9.783876822418936 1108 74-75 9.769575895178725 1108 76-77 9.489361450264074 1108 78-79 9.187479244320752 1108 80-81 9.121309111147678 1108 82-83 8.965874589776735 1108 84-85 8.82230857485964 1108 86-87 8.625865755290569 1108 88-89 8.543810242845609 1108 90-91 8.413322592395165 1108 92-93 8.015129816865795 1108 94-95 7.61999537314672 1108 96-97 7.568051710387515 1108 98-99 7.326416932947907 1108 100-101 6.820012157714547 1109 1 6.726912420546945 1109 2 8.60108471129012 1109 3 8.238541682155748 1109 4 9.60958809007478 1109 5 10.7271230201934 1109 6 9.494790612239438 1109 7 8.00236197021496 1109 8 8.409986340859444 1109 9 7.5280357493866426 1109 10-11 5.955515324168328 1109 12-13 6.543359525037712 1109 14-15 7.857726814588474 1109 16-17 6.945841828953547 1109 18-19 6.592038491205123 1109 20-21 8.988650062405554 1109 22-23 10.064437785233675 1109 24-25 9.639421588898362 1109 26-27 10.049583502598114 1109 28-29 10.250255817102374 1109 30-31 7.847521693824827 1109 32-33 6.89965223229413 1109 34-35 8.810011381337326 1109 36-37 7.11634870688118 1109 38-39 7.644044015255947 1109 40-41 8.409897437446983 1109 42-43 7.17166278166275 1109 44-45 7.8674203301686525 1109 46-47 7.414645621898838 1109 48-49 8.07936163250896 1109 50-51 10.028523339203826 1109 52-53 9.956004085024382 1109 54-55 9.657443151152773 1109 56-57 9.026594608666425 1109 58-59 8.882933096016874 1109 60-61 9.07452255382757 1109 62-63 9.640587181367525 1109 64-65 9.51119285003044 1109 66-67 8.956594461969413 1109 68-69 8.904304074484592 1109 70-71 8.555240056293368 1109 72-73 7.975580959260686 1109 74-75 6.886128639070922 1109 76-77 6.790468416692139 1109 78-79 7.7336364510284135 1109 80-81 7.6965593043795195 1109 82-83 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-21.996319348466358 2314 8 -22.609354318481213 2314 9 -24.23635985500896 2314 10-11 -24.825144016491013 2314 12-13 -24.81532179364361 2314 14-15 -24.89831714145548 2314 16-17 -23.555037291925572 2314 18-19 -22.34158788242125 2314 20-21 -21.972888399132913 2314 22-23 -21.614023753227865 2314 24-25 -21.49969929022252 2314 26-27 -21.257889024874412 2314 28-29 -21.13743649058993 2314 30-31 -21.277533251230114 2314 32-33 -21.21243567979378 2314 34-35 -20.939878728552785 2314 36-37 -20.419255688723215 2314 38-39 -20.4199120287001 2314 40-41 -20.40372893617939 2314 42-43 -20.357567987568018 2314 44-45 -20.00488736213904 2314 46-47 -19.667991740738525 2314 48-49 -19.53206693891961 2314 50-51 -16.86861951793903 2314 52-53 -19.071688222667927 2314 54-55 -19.9572821235725 2314 56-57 -20.028790006718193 2314 58-59 -19.788275695191917 2314 60-61 -19.524818105513088 2314 62-63 -19.117874357094014 2314 64-65 -18.852543413705824 2314 66-67 -18.560987955613005 2314 68-69 -18.1680036178231 2314 70-71 -17.904100603047294 2314 72-73 -17.546616842937116 2314 74-75 -17.13453070158842 2314 76-77 -16.79766345143973 2314 78-79 -16.49163827424631 2314 80-81 -16.142561574741357 2314 82-83 -15.730499372652272 2314 84-85 -15.411854815267052 2314 86-87 -14.983352243835686 2314 88-89 -14.631593863751156 2314 90-91 -14.209001566626242 2314 92-93 -13.716193905283617 2314 94-95 -13.19848431230544 2314 96-97 -12.61849263212013 2314 98-99 -11.970437588677257 2314 100-101 -10.800346077322587 2315 1 -20.393966700332175 2315 2 -18.954080123874714 2315 3 -19.775963812349747 2315 4 -21.215466854980164 2315 5 -20.464525331454954 2315 6 -21.63268191523309 2315 7 -21.996319348466358 2315 8 -22.609354318481213 2315 9 -24.23635985500896 2315 10-11 -24.825144016491013 2315 12-13 -24.81532179364361 2315 14-15 -24.89831714145548 2315 16-17 -23.555037291925572 2315 18-19 -22.34158788242125 2315 20-21 -21.972888399132913 2315 22-23 -21.614023753227865 2315 24-25 -21.49969929022252 2315 26-27 -21.257889024874412 2315 28-29 -21.13743649058993 2315 30-31 -21.277533251230114 2315 32-33 -21.21243567979378 2315 34-35 -20.939878728552785 2315 36-37 -20.419255688723215 2315 38-39 -20.4199120287001 2315 40-41 -20.40372893617939 2315 42-43 -20.357567987568018 2315 44-45 -20.00488736213904 2315 46-47 -19.667991740738525 2315 48-49 -19.53206693891961 2315 50-51 -16.86861951793903 2315 52-53 -19.071688222667927 2315 54-55 -19.9572821235725 2315 56-57 -20.028790006718193 2315 58-59 -19.788275695191917 2315 60-61 -19.524818105513088 2315 62-63 -19.117874357094014 2315 64-65 -18.852543413705824 2315 66-67 -18.560987955613005 2315 68-69 -18.1680036178231 2315 70-71 -17.904100603047294 2315 72-73 -17.546616842937116 2315 74-75 -17.13453070158842 2315 76-77 -16.79766345143973 2315 78-79 -16.49163827424631 2315 80-81 -16.142561574741357 2315 82-83 -15.730499372652272 2315 84-85 -15.411854815267052 2315 86-87 -14.983352243835686 2315 88-89 -14.631593863751156 2315 90-91 -14.209001566626242 2315 92-93 -13.716193905283617 2315 94-95 -13.19848431230544 2315 96-97 -12.61849263212013 2315 98-99 -11.970437588677257 2315 100-101 -10.800346077322587 2316 1 -20.393966700332175 2316 2 -18.954080123874714 2316 3 -19.775963812349747 2316 4 -21.215466854980164 2316 5 -20.464525331454954 2316 6 -21.63268191523309 2316 7 -21.996319348466358 2316 8 -22.609354318481213 2316 9 -24.23635985500896 2316 10-11 -24.825144016491013 2316 12-13 -24.81532179364361 2316 14-15 -24.89831714145548 2316 16-17 -23.555037291925572 2316 18-19 -22.34158788242125 2316 20-21 -21.972888399132913 2316 22-23 -21.614023753227865 2316 24-25 -21.49969929022252 2316 26-27 -21.257889024874412 2316 28-29 -21.13743649058993 2316 30-31 -21.277533251230114 2316 32-33 -21.21243567979378 2316 34-35 -20.939878728552785 2316 36-37 -20.419255688723215 2316 38-39 -20.4199120287001 2316 40-41 -20.40372893617939 2316 42-43 -20.357567987568018 2316 44-45 -20.00488736213904 2316 46-47 -19.667991740738525 2316 48-49 -19.53206693891961 2316 50-51 -16.86861951793903 2316 52-53 -19.071688222667927 2316 54-55 -19.9572821235725 2316 56-57 -20.028790006718193 2316 58-59 -19.788275695191917 2316 60-61 -19.524818105513088 2316 62-63 -19.117874357094014 2316 64-65 -18.852543413705824 2316 66-67 -18.560987955613005 2316 68-69 -18.1680036178231 2316 70-71 -17.904100603047294 2316 72-73 -17.546616842937116 2316 74-75 -17.13453070158842 2316 76-77 -16.79766345143973 2316 78-79 -16.49163827424631 2316 80-81 -16.142561574741357 2316 82-83 -15.730499372652272 2316 84-85 -15.411854815267052 2316 86-87 -14.983352243835686 2316 88-89 -14.631593863751156 2316 90-91 -14.209001566626242 2316 92-93 -13.716193905283617 2316 94-95 -13.19848431230544 2316 96-97 -12.61849263212013 2316 98-99 -11.970437588677257 2316 100-101 -10.800346077322587 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 469187.0 3 17820.0 4 82293.0 5 39887.0 6 15216.0 7 16320.0 8 16600.0 9 13896.0 10 13071.0 11 14890.0 12 16331.0 13 17731.0 14 18660.0 15 20333.0 16 22518.0 17 23038.0 18 21308.0 19 20809.0 20 22098.0 21 23778.0 22 25919.0 23 28945.0 24 31521.0 25 34826.0 26 39236.0 27 43812.0 28 49422.0 29 56431.0 30 64613.0 31 74612.0 32 86930.0 33 103116.0 34 121317.0 35 141781.0 36 152595.0 37 120285.0 38 40207.0 39 1490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.971777509838944 20.80731292792866 13.933420203188954 23.287489359043445 2 14.594824599422203 20.40572210709915 42.68295896012467 22.316494333353972 3 18.775761509461432 26.107119351424142 29.966303414850152 25.15081572426427 4 11.669563324681835 16.991982798138665 40.467708584111364 30.870745293068136 5 12.311966162349345 38.34061021041049 36.15816717778202 13.189256449458146 6 29.38252802767778 39.106454388930885 18.257887662462696 13.253129920928641 7 25.873985013013208 33.118514124148064 23.266431090198274 17.741069772640458 8 24.103423123500832 37.312151363685246 20.724894678518158 17.85953083429576 9 25.042410817591193 16.877670126027557 21.737053462898448 36.342865593482806 10-11 23.783706643094067 27.575560241748438 29.227868130162854 19.412864984994638 12-13 24.344225289424386 24.981110169818393 29.53839442964367 21.136270111113546 14-15 21.515914157525557 26.654231727109917 27.38062341429744 24.449230701067084 16-17 20.694643113983954 29.683838795169464 28.09162339092845 21.52989469991813 18-19 20.42340894306165 28.608414590877462 30.31962134564601 20.648555120414883 20-21 21.34107006546836 27.998662516702115 30.214345493745494 20.445921924084026 22-23 20.829488242020986 27.909060569902945 29.951579605962227 21.309871582113843 24-25 20.705275943640178 28.033468247723697 30.411298351661898 20.849957456974224 26-27 20.69263040440112 28.218172962381754 30.090874931975197 20.998321701241927 28-29 20.570074491849425 28.383705938931925 30.187843148446486 20.858376420772164 30-31 20.997977311832965 28.13499796767233 30.13930682364055 20.727717896854152 32-33 20.833662334631263 28.358421466042465 29.9232943383554 20.88462186097087 34-35 20.863397239429823 28.32092633428074 30.126740046823926 20.688936379465513 36-37 20.911335989427073 28.16298640118592 30.030130264355286 20.895547345031723 38-39 20.82779005798937 28.46539804314059 30.046753568043933 20.660058330826107 40-41 21.049583281040103 28.271844238990145 30.03484666074785 20.64372581922191 42-43 20.850953335979007 28.280249111156287 29.925036748687837 20.94376080417687 44-45 20.891603245432854 28.739150630657402 29.636782887471092 20.732463236438655 46-47 20.93321934330939 28.52626080557239 29.419911715627055 21.120608135491167 48-49 20.9709704101044 28.557539912695283 29.439612522862745 21.031877154337568 50-51 21.288377851728406 28.81574582460466 28.8512373379863 21.044638985680628 52-53 20.73666918133582 29.03085130392639 29.02950255408262 21.202976960655167 54-55 20.798295241145098 28.928653214350557 29.12827568337395 21.144775861130398 56-57 20.85743858473631 29.027649994249742 29.09524678610537 21.019664634908573 58-59 20.811789406424698 28.88578555901111 29.24693720741175 21.055487827152437 60-61 20.84371009210101 29.101666752295756 29.124371279941595 20.930251875661636 62-63 20.76428631294821 28.879570903283465 29.079339973063256 21.276802810705068 64-65 20.502443745040406 28.851652304371555 29.272018041851528 21.37388590873651 66-67 20.621838284558105 28.91734249542699 28.97645571225057 21.484363507764336 68-69 20.627871132502563 28.85582788267922 29.003816251204956 21.51248473361326 70-71 20.73129316784523 28.583189170571693 29.06703967682631 21.618477984756765 72-73 20.719320780160892 28.748561325358057 29.012823339024372 21.51929455545668 74-75 20.647363887932382 28.977745229051795 28.753355010707217 21.621535872308606 76-77 20.58934010273735 28.978171303883876 28.912883799607137 21.519604793771638 78-79 20.42323286062852 28.950679349193003 28.89226934409766 21.733818446080818 80-81 20.519634416362074 28.96131857540342 28.750600894538664 21.76844611369584 82-83 20.77708201913284 28.749290975269766 28.947439349426467 21.52618765617093 84-85 20.888305692936832 28.814767003721908 28.676256344759416 21.62067095858184 86-87 20.530450669009426 28.90245588373016 28.89469623657113 21.672397210689283 88-89 20.75181985954511 28.823316706841005 28.738308856581412 21.686554577032467 90-91 20.739981540132977 28.732729534420283 28.910404268710526 21.616884656736218 92-93 20.702021351374317 28.70275576164465 28.698388516105034 21.896834370875997 94-95 20.43553652319843 28.722797693831346 28.896462404028362 21.945203378941862 96-97 20.542563327452832 28.644762372817706 28.8346520032733 21.978022296456164 98-99 20.416834340623545 28.596638735229714 28.56715496582903 22.419371958317715 100-101 20.6041818259767 28.71233517095003 28.423478739379675 22.26000426369359 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 123128.0 1 111921.5 2 74707.0 3 37825.0 4 23224.5 5 18501.5 6 17344.5 7 17612.5 8 18247.5 9 18027.5 10 16545.0 11 14035.0 12 11202.0 13 8492.0 14 6395.5 15 5321.5 16 5062.0 17 5430.5 18 6275.0 19 7438.0 20 8575.5 21 9734.5 22 11201.0 23 12789.5 24 14242.0 25 16163.5 26 18850.0 27 21624.5 28 24334.5 29 27303.0 30 30626.5 31 34414.5 32 38794.5 33 43174.0 34 47409.0 35 51589.0 36 56386.5 37 60922.0 38 63266.0 39 63739.0 40 63685.0 41 63539.0 42 63405.0 43 63921.5 44 64243.5 45 64926.5 46 64442.0 47 62532.5 48 60510.5 49 57407.5 50 53515.5 51 49511.5 52 45079.5 53 40258.5 54 35327.5 55 29709.5 56 24336.5 57 19355.0 58 15167.5 59 12139.5 60 9401.5 61 6876.0 62 4774.5 63 3141.0 64 2027.0 65 1258.5 66 757.5 67 474.5 68 290.5 69 169.5 70 103.0 71 71.5 72 51.0 73 34.0 74 21.0 75 14.0 76 13.5 77 10.0 78 6.0 79 4.5 80 4.0 81 2.5 82 2.5 83 2.5 84 1.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.5881822575585 2 28.48351408159439 3 22.343019405118234 4 28.58088355139007 5 31.629344058578074 6 24.405443268976214 7 23.294055798782953 8 19.402762899923783 9 11.447625400288858 10-11 15.002859374367002 12-13 15.118718208891666 14-15 19.196624148193788 16-17 22.37919732132679 18-19 26.24747861593091 20-21 28.291318901736446 22-23 27.87155143906141 24-25 26.4495190880904 26-27 27.850942274554587 28-29 24.896176917547326 30-31 26.6974414487748 32-33 25.724971524022983 34-35 23.842895514597885 36-37 26.753145076270396 38-39 26.881934689439912 40-41 23.9935190654792 42-43 24.80290572732215 44-45 23.585622481560097 46-47 26.771657994330244 48-49 22.23234230338386 50-51 27.863119346611757 52-53 21.416125175590082 54-55 22.551772576574233 56-57 24.632732911822924 58-59 23.917512466778028 60-61 23.44100503004934 62-63 23.2806539535208 64-65 21.84856433027046 66-67 22.34318427843429 68-69 23.302417231240007 70-71 21.836222384897226 72-73 21.68260756099606 74-75 19.081754553565457 76-77 21.2453870801501 78-79 20.40015224873071 80-81 21.40978932958741 82-83 22.22522919746265 84-85 19.244649389827412 86-87 20.777076202562412 88-89 19.621290703688736 90-91 20.739791279803207 92-93 20.72028912184703 94-95 19.818314316373993 96-97 18.71858574495888 98-99 18.117104334660798 100-101 19.789178846094057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2122842.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.48950711418289 #Duplication Level Percentage of deduplicated Percentage of total 1 84.46873733681436 59.541596614294214 2 10.264912821078527 14.471372906557637 3 2.617499418792035 5.5351873170693215 4 1.012824370473625 2.8557396267167356 5 0.49483562354752747 1.7440359603202282 6 0.28295625423200593 1.1967268137413711 7 0.1744695500832994 0.8608790814263513 8 0.12140046855374914 0.6845967353427708 9 0.08728976869895312 0.5537711494530211 >10 0.4422677368558054 5.48522394762572 >50 0.022411287359517997 1.0602994933299341 >100 0.009910608394970736 1.2053199032348936 >500 2.0775219249284007E-4 0.08640166293919568 >1k 2.0775219249283774E-4 0.24993023403880069 >5k 0.0 0.0 >10k+ 6.925073083094591E-5 4.4689185539097975 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 91549 4.312567774709564 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2356 0.11098329503561734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.7106661729888515E-5 0.0 7 0.0 0.0 0.0 4.7106661729888515E-5 0.0 8 0.0 0.0 0.0 4.7106661729888515E-5 0.0 9 0.0 0.0 0.0 4.7106661729888515E-5 0.0 10-11 0.0 0.0 0.0 4.7106661729888515E-5 0.0 12-13 0.0 0.0 0.0 4.7106661729888515E-5 0.0 14-15 0.0 0.0 0.0 9.421332345977703E-5 0.0 16-17 0.0 0.0 0.0 9.421332345977703E-5 0.0 18-19 0.0 0.0 0.0 9.421332345977703E-5 0.0 20-21 0.0 0.0 0.0 1.1776665432472129E-4 0.0 22-23 0.0 0.0 0.0 2.3553330864944258E-4 0.0 24-25 0.0 0.0 0.0 2.826399703793311E-4 0.0 26-27 0.0 0.0 0.0 3.768532938391081E-4 0.0 28-29 0.0 0.0 0.0 7.065999259483278E-4 0.0 30-31 0.0 0.0 0.0 0.0023082264247645374 0.0 32-33 0.0 0.0 0.0 0.0063829526643998935 0.0 34-35 0.0 0.0 0.0 0.011423365469497966 0.0 36-37 0.0 0.0 0.0 0.021033124462395223 0.0 38-39 0.0 0.0 0.0 0.03801507601602003 0.0 40-41 0.0 0.0 0.0 0.056622207399326 0.0 42-43 0.0 0.0 0.0 0.07438141887149397 0.0 44-45 0.0 0.0 0.0 0.09588560995118808 0.0 46-47 0.0 0.0 0.0 0.12202980721127621 0.0 48-49 0.0 0.0 0.0 0.15611147697285055 0.0 50-51 0.0 2.3553330864944258E-5 0.0 0.19389101968022113 0.0 52-53 0.0 4.7106661729888515E-5 0.0 0.23753534177296284 0.0 54-55 0.0 4.7106661729888515E-5 0.0 0.28252220372500636 0.0 56-57 0.0 4.7106661729888515E-5 0.0 0.33803740457368 0.0 58-59 0.0 4.7106661729888515E-5 0.0 0.4049995242227165 0.0 60-61 0.0 4.7106661729888515E-5 0.0 0.47900408980037135 0.0 62-63 0.0 4.7106661729888515E-5 0.0 0.5534326153335952 0.0 64-65 0.0 9.421332345977703E-5 0.0 0.6291094674026612 0.0 66-67 0.0 9.421332345977703E-5 0.0 0.7199546645487511 0.0 68-69 0.0 9.421332345977703E-5 0.0 0.8184075875642182 0.0 70-71 0.0 9.421332345977703E-5 0.0 0.9347374887061779 0.0 72-73 0.0 9.421332345977703E-5 0.0 1.061878368715147 0.0 74-75 0.0 9.421332345977703E-5 0.0 1.1842850292202622 0.0 76-77 0.0 9.421332345977703E-5 0.0 1.3172200286220077 0.0 78-79 0.0 9.421332345977703E-5 0.0 1.4771235918641143 0.0 80-81 0.0 9.421332345977703E-5 0.0 1.6560817997759607 0.0 82-83 0.0 9.421332345977703E-5 0.0 1.842365093586805 0.0 84-85 0.0 9.421332345977703E-5 0.0 2.023019141320927 0.0 86-87 0.0 9.421332345977703E-5 0.0 2.2096321817638804 0.0 88-89 0.0 9.421332345977703E-5 0.0 2.4210233262767553 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1695 0.0 57.93443 1 GTATCAA 3405 0.0 47.164776 1 ATCAACG 4225 0.0 37.855076 3 TCAACGC 4460 0.0 36.077187 4 CAACGCA 4435 0.0 35.935406 5 TATCAAC 4565 0.0 34.729244 2 AACGCAG 4995 0.0 33.354874 6 ACGCAGA 5630 0.0 28.522991 7 CGCAGAG 5725 0.0 28.11811 8 GCAGAGT 6405 0.0 24.518227 9 ACATGGG 4020 0.0 24.396769 3 GTACATG 4255 0.0 24.160267 1 TACATGG 4125 0.0 23.928072 2 AGAGTAC 5525 0.0 22.2449 10-11 GTACTAG 605 0.0 21.979774 1 GAGTACA 2970 0.0 21.525776 1 GCGGTCG 235 0.0 21.008745 22-23 AGTACTT 4520 0.0 20.976772 12-13 GAGTACT 4155 0.0 20.566433 12-13 CATGGGT 1000 0.0 20.113031 4 >>END_MODULE