FastQCFastQC Report
Sat 18 Jun 2016
SRR1930974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122842
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60410.28457134351025654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.2354862019877127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.15644122360495977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.057.0080641
GTATCAA58050.042.5780031
ATCAACG76050.031.93563
CAACGCA76550.031.670215
TCAACGC78600.031.080934
TATCAAC78250.030.9791832
AACGCAG81500.030.152756
TATAGCG700.00844215127.1104775
ACGCAGA89800.026.8897027
CGCAGAG90100.026.642218
GCAGAGT101200.023.1574639
GTGGTAT15950.022.946381
TGGTATC15750.022.6341862
GGCGTCG3700.021.7967518
GAGTACA44550.021.4453181
TACATGG60150.021.3359642
AGAGTAC95500.021.11203610-11
ACATGGG58200.020.9876383
GTACATG63050.020.9577121
ACGGGCG3850.020.9490075
Produced by FastQC (version 0.11.5)