FastQCFastQC Report
Sat 18 Jun 2016
SRR1930973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794755
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN780294.347612905382628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19390.10803703012388878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.058.6322331
GTATCAA31250.045.4944421
ATCAACG36800.037.9874083
TCAACGC37900.037.2782824
CAACGCA39100.036.3823435
TATCAAC39100.035.5669022
AACGCAG43000.033.6475646
GAGTACG1556.148184E-1032.9937631
ACGCAGA47600.030.1125347
CGCAGAG48600.028.8259568
GTACATG36750.026.1615871
TACATGG36300.026.0566772
GCAGAGT54250.025.6276449
ACATGGG36200.025.0038363
GTGGTAT8650.024.2399251
TGGTATC8500.023.4584642
GTGTAGC6650.021.5327721
GAGTACT35700.021.32506812-13
CAGAGTA53400.020.4853610-11
AGAGTAC49950.020.29332510-11
Produced by FastQC (version 0.11.5)