##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930973_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1794755 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.96439848335845 31.0 10.0 31.0 2.0 33.0 2 21.49583592189463 30.0 2.0 31.0 2.0 34.0 3 22.31949597577385 30.0 10.0 31.0 2.0 34.0 4 23.819007051101682 33.0 2.0 37.0 2.0 37.0 5 23.13164136609175 33.0 2.0 37.0 2.0 37.0 6 24.276106766661744 33.0 2.0 37.0 2.0 37.0 7 24.642271507810257 33.0 2.0 37.0 2.0 37.0 8 25.201106000540463 33.0 10.0 37.0 2.0 37.0 9 26.862277023883482 35.0 17.0 39.0 2.0 39.0 10-11 27.427556128830954 35.0 17.0 39.0 2.0 39.0 12-13 27.42123103153355 35.0 14.0 39.0 2.0 39.0 14-15 27.497592150460648 36.0 10.0 40.0 2.0 41.0 16-17 26.201830890567237 35.5 2.0 39.0 2.0 41.0 18-19 24.984545522926528 34.0 2.0 39.0 2.0 41.0 20-21 24.602948313279526 33.5 2.0 39.0 2.0 40.0 22-23 24.246096263835454 33.0 2.0 39.0 2.0 40.0 24-25 24.131702934383803 33.0 2.0 39.0 2.0 40.0 26-27 23.885690804594496 32.5 2.0 39.0 2.0 40.0 28-29 23.777539552752327 32.0 2.0 38.0 2.0 40.0 30-31 23.9170636103535 32.0 2.0 38.0 2.0 40.0 32-33 23.86416223941429 32.5 2.0 38.0 2.0 40.0 34-35 23.59220896445476 31.5 2.0 38.0 2.0 40.0 36-37 23.063656042189603 31.0 2.0 38.0 2.0 40.0 38-39 23.06705177029734 30.5 2.0 38.0 2.0 40.0 40-41 23.05395137497876 31.0 2.0 38.0 2.0 40.0 42-43 22.971787514173243 31.0 2.0 38.0 2.0 40.0 44-45 22.62173527863135 30.0 2.0 38.0 2.0 40.0 46-47 22.306258792982888 30.0 2.0 38.0 2.0 40.0 48-49 22.19056305735323 30.0 2.0 38.0 2.0 40.0 50-51 19.389413318252352 20.0 2.0 36.0 2.0 39.0 52-53 21.647252967675254 28.5 2.0 37.0 2.0 39.0 54-55 22.599805265899803 31.0 2.0 38.0 2.0 40.0 56-57 22.65684452752604 31.0 2.0 38.0 2.0 40.0 58-59 22.398984541065495 31.0 2.0 38.0 2.0 40.0 60-61 22.153210048168134 30.0 2.0 38.0 2.0 40.0 62-63 21.73834729531329 29.5 2.0 37.5 2.0 40.0 64-65 21.46295343932737 29.0 2.0 37.0 2.0 40.0 66-67 21.18394989845411 28.0 2.0 36.5 2.0 39.0 68-69 20.773003278999084 28.0 2.0 36.0 2.0 39.0 70-71 20.487931778989335 28.0 2.0 35.5 2.0 39.0 72-73 20.1105334711423 26.5 2.0 35.0 2.0 38.5 74-75 19.70099150023262 26.0 2.0 35.0 2.0 38.0 76-77 19.357308100548543 26.0 2.0 34.0 2.0 37.0 78-79 19.03945246008508 25.5 2.0 34.0 2.0 36.5 80-81 18.66187501915303 25.0 2.0 34.0 2.0 36.0 82-83 18.259448782702933 23.5 2.0 34.0 2.0 35.5 84-85 17.931856158640038 21.5 2.0 34.0 2.0 35.0 86-87 17.489495223582047 20.0 2.0 33.0 2.0 35.0 88-89 17.14083816454056 18.5 2.0 33.0 2.0 35.0 90-91 16.712149569161248 14.5 2.0 32.5 2.0 35.0 92-93 16.20874631913548 7.0 2.0 32.0 2.0 35.0 94-95 15.69121439973701 2.0 2.0 32.0 2.0 34.0 96-97 15.076460296809312 2.0 2.0 32.0 2.0 34.0 98-99 14.396731865909274 2.0 2.0 31.0 2.0 34.0 100-101 13.202752186231546 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.001783462176387 1101 2 9.907965367651851 1101 3 9.204527847870086 1101 4 11.260533267482305 1101 5 12.019195549388055 1101 6 11.142151515455506 1101 7 10.568602824703245 1101 8 10.104576692666097 1101 9 9.886800599947492 1101 10-11 9.125554344573853 1101 12-13 8.957690195598758 1101 14-15 9.879262778458354 1101 16-17 11.305222174861743 1101 18-19 12.44137056905436 1101 20-21 12.574696006291564 1101 22-23 12.851267858470347 1101 24-25 12.758074062572078 1101 26-27 12.90731589800377 1101 28-29 12.905368543502071 1101 30-31 12.40101316990927 1101 32-33 12.388609362170076 1101 34-35 12.318558430333773 1101 36-37 12.862909327771764 1101 38-39 12.55581249770838 1101 40-41 12.115380137302289 1101 42-43 11.727607876867818 1101 44-45 11.91716419222341 1101 46-47 11.894724411761082 1101 48-49 11.725536489271182 1101 50-51 13.882415260672413 1101 52-53 12.582190652469428 1101 54-55 12.187368173770516 1101 56-57 12.082910592714097 1101 58-59 12.085216295173382 1101 60-61 11.891551403927547 1101 62-63 11.948718069839131 1101 64-65 11.972768850195223 1101 66-67 11.478483816598558 1101 68-69 11.602188779413886 1101 70-71 11.410649055820219 1101 72-73 11.222399569355293 1101 74-75 11.305016353353505 1101 76-77 11.274078167324486 1101 78-79 10.959342761332458 1101 80-81 10.600925952415412 1101 82-83 10.457761129637053 1101 84-85 10.328784854882542 1101 86-87 10.364244156674197 1101 88-89 10.168847785949396 1101 90-91 9.79831515844791 1101 92-93 9.661568168506614 1101 94-95 9.5914065668994 1101 96-97 9.550044344433564 1101 98-99 9.326979547251804 1101 100-101 8.639828276005211 1102 1 7.795309473867238 1102 2 9.37270648918085 1102 3 8.74583649351484 1102 4 10.727379732756514 1102 5 11.685128723300704 1102 6 10.593748311730284 1102 7 10.18859053039645 1102 8 9.527863370681356 1102 9 9.169564026861664 1102 10-11 8.43388587654356 1102 12-13 8.584034463209335 1102 14-15 9.340208089058478 1102 16-17 10.382933283420915 1102 18-19 11.811527152588095 1102 20-21 12.004421151976846 1102 22-23 12.125934437320534 1102 24-25 11.954941153459878 1102 26-27 12.063272050561256 1102 28-29 11.870930974262151 1102 30-31 11.89295460082247 1102 32-33 11.789606979866672 1102 34-35 11.584476855581684 1102 36-37 12.207871870666732 1102 38-39 11.993301802111816 1102 40-41 11.503121688906852 1102 42-43 11.21309367086388 1102 44-45 11.223236420149952 1102 46-47 11.24898367977697 1102 48-49 10.74931814823702 1102 50-51 12.822333872811797 1102 52-53 11.685122540658789 1102 54-55 11.141869950365404 1102 56-57 11.100703280528773 1102 58-59 11.3017956519965 1102 60-61 11.174277959179872 1102 62-63 11.285719711331744 1102 64-65 11.232162961537504 1102 66-67 11.062065681820528 1102 68-69 10.963283659488102 1102 70-71 10.643663322620188 1102 72-73 10.744540298637357 1102 74-75 10.32929310585515 1102 76-77 10.042557062097828 1102 78-79 9.77184810913074 1102 80-81 9.465851263375814 1102 82-83 9.478013241735912 1102 84-85 9.305167570348146 1102 86-87 9.398024791114473 1102 88-89 9.148091010570433 1102 90-91 8.881381383330147 1102 92-93 8.723084455085553 1102 94-95 8.639173664091972 1102 96-97 8.49790302970832 1102 98-99 8.222070381012964 1102 100-101 7.313952588109473 1103 1 8.109910314203372 1103 2 9.743504808508579 1103 3 8.999512964103076 1103 4 11.276224270571642 1103 5 12.072208555233477 1103 6 11.086920580637848 1103 7 10.534178765690573 1103 8 9.852968412698157 1103 9 9.69529932097931 1103 10-11 8.868234616039356 1103 12-13 8.822742446402614 1103 14-15 9.830229097461839 1103 16-17 11.035244207790665 1103 18-19 12.08621202653767 1103 20-21 12.416711067943229 1103 22-23 12.634600403707093 1103 24-25 12.619069304720384 1103 26-27 12.48659137829235 1103 28-29 12.176865848211733 1103 30-31 11.922628970570372 1103 32-33 12.109459921043136 1103 34-35 12.02749156146404 1103 36-37 12.3541428790701 1103 38-39 12.071295499590804 1103 40-41 11.836630092268194 1103 42-43 11.529270141452113 1103 44-45 11.631324655444068 1103 46-47 11.553080318432436 1103 48-49 11.479885375127775 1103 50-51 13.474907402223561 1103 52-53 12.01101539780165 1103 54-55 11.663403563810775 1103 56-57 11.512467986411128 1103 58-59 11.421543551156162 1103 60-61 11.477586782709292 1103 62-63 11.48136046763426 1103 64-65 11.01787724725179 1103 66-67 10.962800975938176 1103 68-69 10.764229037639364 1103 70-71 10.648915423460526 1103 72-73 10.524477273427273 1103 74-75 10.333494786527417 1103 76-77 10.053323868820517 1103 78-79 9.678885924256793 1103 80-81 9.448256725560686 1103 82-83 9.33331786358465 1103 84-85 9.136049923289324 1103 86-87 8.888045799517833 1103 88-89 8.845044792083037 1103 90-91 8.610635585010815 1103 92-93 8.533746219791437 1103 94-95 8.38050769770542 1103 96-97 8.428837903657895 1103 98-99 8.055316179332289 1103 100-101 7.50066477298342 1104 1 7.899092999188294 1104 2 9.720148327127834 1104 3 8.90681406424277 1104 4 11.16420900874449 1104 5 12.005784030996029 1104 6 10.932628490938168 1104 7 10.48493576450252 1104 8 9.983395402240742 1104 9 9.730238711028441 1104 10-11 8.982311279848329 1104 12-13 8.7456289041965 1104 14-15 9.662795876115297 1104 16-17 10.913415731449323 1104 18-19 11.910122494401666 1104 20-21 12.16416811005578 1104 22-23 12.572842932722846 1104 24-25 12.538073124528992 1104 26-27 12.505705516697585 1104 28-29 12.14393468636326 1104 30-31 11.873643542634312 1104 32-33 12.10031403068285 1104 34-35 11.939331200219591 1104 36-37 12.272910476735785 1104 38-39 11.789611133716022 1104 40-41 11.541930253503068 1104 42-43 11.10968045809042 1104 44-45 11.160050328779178 1104 46-47 11.299861196759942 1104 48-49 11.2226639404638 1104 50-51 13.132474235274753 1104 52-53 11.8601329578241 1104 54-55 11.247783282813863 1104 56-57 11.155200966449332 1104 58-59 11.178082838284794 1104 60-61 10.990439878375309 1104 62-63 11.052506986832313 1104 64-65 10.922905338239609 1104 66-67 10.896273775985176 1104 68-69 10.64458707805905 1104 70-71 10.436485759725532 1104 72-73 10.33501011551284 1104 74-75 10.196645872958882 1104 76-77 9.962976038854766 1104 78-79 9.619113562437906 1104 80-81 9.484190098132917 1104 82-83 9.254088385672304 1104 84-85 8.649393550486451 1104 86-87 8.42744009167281 1104 88-89 8.228929029361588 1104 90-91 8.190854829622179 1104 92-93 7.826505283541225 1104 94-95 7.7933561423655195 1104 96-97 7.31984579568058 1104 98-99 7.076767516210902 1104 100-101 6.363984356383845 1105 1 8.078162597065976 1105 2 9.617274262138409 1105 3 8.906050217993801 1105 4 11.123443122011135 1105 5 11.426260825961261 1105 6 10.81882074142418 1105 7 10.534406293524807 1105 8 9.968986751441602 1105 9 9.688907684902496 1105 10-11 9.059128572900075 1105 12-13 8.612501987403128 1105 14-15 9.625933751164467 1105 16-17 10.7952431080728 1105 18-19 11.634203334974405 1105 20-21 11.907081900391734 1105 22-23 12.470543354894783 1105 24-25 12.525662492537023 1105 26-27 12.523262228943079 1105 28-29 12.4832159604588 1105 30-31 12.006125997539996 1105 32-33 12.04686322171359 1105 34-35 12.006264162562246 1105 36-37 12.114307685064698 1105 38-39 12.014018211419213 1105 40-41 11.96643566973384 1105 42-43 11.62756500994102 1105 44-45 11.551451957267325 1105 46-47 11.557372062205758 1105 48-49 11.328675116656154 1105 50-51 13.36053295208249 1105 52-53 11.97978720255243 1105 54-55 11.678480126926999 1105 56-57 11.435989245095104 1105 58-59 11.42319976418289 1105 60-61 11.142423114353857 1105 62-63 11.357488626479025 1105 64-65 11.022716005859927 1105 66-67 10.951689085333559 1105 68-69 10.811809847941824 1105 70-71 10.604456484650346 1105 72-73 10.452243822285084 1105 74-75 10.371746007593462 1105 76-77 10.14862579825655 1105 78-79 10.086995964301689 1105 80-81 9.796098434446407 1105 82-83 9.537093687993835 1105 84-85 9.561323146296939 1105 86-87 9.611193722947132 1105 88-89 9.503847843989721 1105 90-91 9.17221978547801 1105 92-93 8.6543719971782 1105 94-95 8.46918193093662 1105 96-97 8.088279929565731 1105 98-99 7.425768466745447 1105 100-101 6.781727062520176 1106 1 7.8549828886877116 1106 2 9.468774098042577 1106 3 8.794144362116825 1106 4 10.998122666293565 1106 5 11.638392665241117 1106 6 10.612371306382688 1106 7 9.683926664850237 1106 8 9.222202560902897 1106 9 8.98380008492125 1106 10-11 8.444798787162345 1106 12-13 8.28866777107794 1106 14-15 9.084214964535938 1106 16-17 10.613988478226116 1106 18-19 11.839995448997566 1106 20-21 11.887930510265615 1106 22-23 12.01155418521969 1106 24-25 11.929786455216941 1106 26-27 12.017117543303808 1106 28-29 11.981908819945886 1106 30-31 11.449662965986718 1106 32-33 11.689394985978208 1106 34-35 11.311233922044636 1106 36-37 11.869870533768339 1106 38-39 11.360731137482333 1106 40-41 11.435651291657045 1106 42-43 11.035325336257305 1106 44-45 10.83650704658234 1106 46-47 10.798375391006921 1106 48-49 10.972966925929914 1106 50-51 13.033368059213633 1106 52-53 11.787207147228692 1106 54-55 11.14799580980008 1106 56-57 11.098662600620447 1106 58-59 11.198232817771903 1106 60-61 11.035233841532818 1106 62-63 10.94535397412777 1106 64-65 10.93036005855813 1106 66-67 10.651358080598218 1106 68-69 10.499166967356704 1106 70-71 10.258617486103763 1106 72-73 9.938847976253857 1106 74-75 9.570274771248577 1106 76-77 9.616427382953443 1106 78-79 9.339350973913017 1106 80-81 9.079726358868555 1106 82-83 9.019381652736673 1106 84-85 8.69358907531377 1106 86-87 8.460051147111415 1106 88-89 8.240396205375628 1106 90-91 7.928893797386674 1106 92-93 7.81693376753781 1106 94-95 7.674618660272264 1106 96-97 7.192315749341626 1106 98-99 6.970061213709679 1106 100-101 6.588658470462413 1107 1 7.545875737371077 1107 2 8.888574183314262 1107 3 8.645334984938508 1107 4 10.59441619038282 1107 5 11.43910515754298 1107 6 10.247380882528272 1107 7 9.996720530702984 1107 8 8.770475944632249 1107 9 6.125867314200569 1107 10-11 7.7814654763876625 1107 12-13 7.688411414615224 1107 14-15 8.455898268204649 1107 16-17 9.732959803930111 1107 18-19 11.24988845333559 1107 20-21 11.692560627412664 1107 22-23 11.230236534657646 1107 24-25 11.456830979218218 1107 26-27 11.542670760279645 1107 28-29 11.596358039303908 1107 30-31 11.080352651496625 1107 32-33 11.318115843026316 1107 34-35 11.357687364381349 1107 36-37 11.79547234206947 1107 38-39 11.435683053726585 1107 40-41 11.0293330658495 1107 42-43 10.72809253778949 1107 44-45 10.370502837138844 1107 46-47 10.543883941862006 1107 48-49 10.883772066594357 1107 50-51 12.534475914360048 1107 52-53 11.105696078828622 1107 54-55 10.711211229086373 1107 56-57 10.582416855745212 1107 58-59 10.627576022769091 1107 60-61 10.573272998832007 1107 62-63 10.719240129660612 1107 64-65 10.678351233615487 1107 66-67 10.666765997329744 1107 68-69 10.33121166054783 1107 70-71 10.139564001807965 1107 72-73 9.981844802252251 1107 74-75 10.162498534978333 1107 76-77 9.928993586942262 1107 78-79 9.600830099269164 1107 80-81 9.323072122016296 1107 82-83 9.344697597865018 1107 84-85 9.173443978097282 1107 86-87 8.785466440520455 1107 88-89 8.72118990880612 1107 90-91 8.421678188607753 1107 92-93 8.101466025274858 1107 94-95 8.122882298666179 1107 96-97 7.7648741029213415 1107 98-99 7.73579864459562 1107 100-101 7.388734402867684 1108 1 7.333290613037857 1108 2 8.647014233489127 1108 3 8.306519043824153 1108 4 10.061901060687585 1108 5 11.663350573344399 1108 6 10.185516071601949 1108 7 9.410430249212066 1108 8 6.6847742985289536 1108 9 7.8580011451130005 1108 10-11 6.412594354243996 1108 12-13 5.470262492962362 1108 14-15 7.755789194754769 1108 16-17 9.213449782571022 1108 18-19 9.313122074053833 1108 20-21 9.624722926333764 1108 22-23 10.65104276699488 1108 24-25 10.286383495011094 1108 26-27 8.911815509589982 1108 28-29 10.6340881253792 1108 30-31 10.71183333571011 1108 32-33 11.114716369199225 1108 34-35 10.795873235137137 1108 36-37 10.692784918917592 1108 38-39 10.3077339122554 1108 40-41 10.547894557961357 1108 42-43 9.845864905327655 1108 44-45 9.47722929381261 1108 46-47 10.423479092272032 1108 48-49 10.738385056686898 1108 50-51 12.76720080691084 1108 52-53 11.28906590926335 1108 54-55 10.660628753679795 1108 56-57 10.649568332240097 1108 58-59 10.759857504518166 1108 60-61 10.600591908194556 1108 62-63 10.530566919485565 1108 64-65 10.501996502817441 1108 66-67 9.979557568642509 1108 68-69 10.116553574541552 1108 70-71 10.282475831586527 1108 72-73 10.02601065320443 1108 74-75 9.954925640296757 1108 76-77 9.55680362337107 1108 78-79 9.29665158328812 1108 80-81 9.249165559088926 1108 82-83 9.392700294777509 1108 84-85 9.241378998789159 1108 86-87 9.014390206913486 1108 88-89 8.919897418079117 1108 90-91 8.52664979203869 1108 92-93 8.400901760227994 1108 94-95 8.402697348268179 1108 96-97 8.075147630342157 1108 98-99 7.802997837355042 1108 100-101 7.130410003978042 1109 1 6.92779192644953 1109 2 8.910128672114883 1109 3 8.546440733112043 1109 4 9.896903549628494 1109 5 11.010269036508884 1109 6 9.787614761773053 1109 7 8.597600776677549 1109 8 8.916990585050836 1109 9 7.99489778051003 1109 10-11 6.619003417788807 1109 12-13 6.8259231914183545 1109 14-15 8.298708225734345 1109 16-17 7.505455206670806 1109 18-19 7.292217277536288 1109 20-21 9.428383352281944 1109 22-23 10.503717279208598 1109 24-25 10.070707265780982 1109 26-27 10.519932849718277 1109 28-29 10.585523107022922 1109 30-31 8.552021887233359 1109 32-33 7.479513136453125 1109 34-35 9.21532966890009 1109 36-37 7.804538908415726 1109 38-39 8.229201041135859 1109 40-41 9.057217500893767 1109 42-43 7.72901062263934 1109 44-45 8.541431890888092 1109 46-47 7.718844722305214 1109 48-49 8.625674711323953 1109 50-51 10.748906141278471 1109 52-53 10.717377218500545 1109 54-55 10.657474421429797 1109 56-57 9.595572699964965 1109 58-59 9.604431016215642 1109 60-61 9.883908702872947 1109 62-63 10.537096114463822 1109 64-65 10.53922734027956 1109 66-67 10.077887291156813 1109 68-69 9.449996064807 1109 70-71 8.999321483051201 1109 72-73 8.68466882906317 1109 74-75 7.665593995570951 1109 76-77 7.689046862110171 1109 78-79 8.55221302267223 1109 80-81 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56-57 -20.03873999678215 2313 58-59 -19.786702247163163 2313 60-61 -19.547623301324325 2313 62-63 -19.15256810379431 2313 64-65 -18.885484097286025 2313 66-67 -18.60994062070048 2313 68-69 -18.199794071604334 2313 70-71 -17.91410978662351 2313 72-73 -17.562970205564323 2313 74-75 -17.175958994985187 2313 76-77 -16.852663526137466 2313 78-79 -16.540389285827242 2313 80-81 -16.167026887884692 2313 82-83 -15.777501464146443 2313 84-85 -15.44667066494597 2313 86-87 -15.03527352821326 2313 88-89 -14.700123275143852 2313 90-91 -14.263313994821115 2313 92-93 -13.769299998695958 2313 94-95 -13.278368352714748 2313 96-97 -12.680931003905128 2313 98-99 -12.021627097978635 2313 100-101 -10.8856272438233 2314 1 -20.448913215208393 2314 2 -18.963953174684697 2314 3 -19.778323170350706 2314 4 -21.216422788251887 2314 5 -20.456152789304337 2314 6 -21.651524797513414 2314 7 -22.024644763721195 2314 8 -22.633901335201 2314 9 -24.28736874624758 2314 10-11 -24.899097316733755 2314 12-13 -24.896007553597386 2314 14-15 -24.959161658840685 2314 16-17 -23.603673859436224 2314 18-19 -22.340783771781656 2314 20-21 -21.95960195726685 2314 22-23 -21.597017243351733 2314 24-25 -21.469953804523314 2314 26-27 -21.238986352800087 2314 28-29 -21.12276650472944 2314 30-31 -21.2659214495977 2314 32-33 -21.229825793242572 2314 34-35 -20.96279205198134 2314 36-37 -20.41844115454335 2314 38-39 -20.44472340797222 2314 40-41 -20.443569487563735 2314 42-43 -20.368051287119318 2314 44-45 -20.03647996640467 2314 46-47 -19.723442790811262 2314 48-49 -19.582877229914242 2314 50-51 -16.904294278448706 2314 52-53 -19.076169476147932 2314 54-55 -19.987069125264853 2314 56-57 -20.03873999678215 2314 58-59 -19.786702247163163 2314 60-61 -19.547623301324325 2314 62-63 -19.15256810379431 2314 64-65 -18.885484097286025 2314 66-67 -18.60994062070048 2314 68-69 -18.199794071604334 2314 70-71 -17.91410978662351 2314 72-73 -17.562970205564323 2314 74-75 -17.175958994985187 2314 76-77 -16.852663526137466 2314 78-79 -16.540389285827242 2314 80-81 -16.167026887884692 2314 82-83 -15.777501464146443 2314 84-85 -15.44667066494597 2314 86-87 -15.03527352821326 2314 88-89 -14.700123275143852 2314 90-91 -14.263313994821115 2314 92-93 -13.769299998695958 2314 94-95 -13.278368352714748 2314 96-97 -12.680931003905128 2314 98-99 -12.021627097978635 2314 100-101 -10.8856272438233 2315 1 -20.448913215208393 2315 2 -18.963953174684697 2315 3 -19.778323170350706 2315 4 -21.216422788251887 2315 5 -20.456152789304337 2315 6 -21.651524797513414 2315 7 -22.024644763721195 2315 8 -22.633901335201 2315 9 -24.28736874624758 2315 10-11 -24.899097316733755 2315 12-13 -24.896007553597386 2315 14-15 -24.959161658840685 2315 16-17 -23.603673859436224 2315 18-19 -22.340783771781656 2315 20-21 -21.95960195726685 2315 22-23 -21.597017243351733 2315 24-25 -21.469953804523314 2315 26-27 -21.238986352800087 2315 28-29 -21.12276650472944 2315 30-31 -21.2659214495977 2315 32-33 -21.229825793242572 2315 34-35 -20.96279205198134 2315 36-37 -20.41844115454335 2315 38-39 -20.44472340797222 2315 40-41 -20.443569487563735 2315 42-43 -20.368051287119318 2315 44-45 -20.03647996640467 2315 46-47 -19.723442790811262 2315 48-49 -19.582877229914242 2315 50-51 -16.904294278448706 2315 52-53 -19.076169476147932 2315 54-55 -19.987069125264853 2315 56-57 -20.03873999678215 2315 58-59 -19.786702247163163 2315 60-61 -19.547623301324325 2315 62-63 -19.15256810379431 2315 64-65 -18.885484097286025 2315 66-67 -18.60994062070048 2315 68-69 -18.199794071604334 2315 70-71 -17.91410978662351 2315 72-73 -17.562970205564323 2315 74-75 -17.175958994985187 2315 76-77 -16.852663526137466 2315 78-79 -16.540389285827242 2315 80-81 -16.167026887884692 2315 82-83 -15.777501464146443 2315 84-85 -15.44667066494597 2315 86-87 -15.03527352821326 2315 88-89 -14.700123275143852 2315 90-91 -14.263313994821115 2315 92-93 -13.769299998695958 2315 94-95 -13.278368352714748 2315 96-97 -12.680931003905128 2315 98-99 -12.021627097978635 2315 100-101 -10.8856272438233 2316 1 -20.448913215208393 2316 2 -18.963953174684697 2316 3 -19.778323170350706 2316 4 -21.216422788251887 2316 5 -20.456152789304337 2316 6 -21.651524797513414 2316 7 -22.024644763721195 2316 8 -22.633901335201 2316 9 -24.28736874624758 2316 10-11 -24.899097316733755 2316 12-13 -24.896007553597386 2316 14-15 -24.959161658840685 2316 16-17 -23.603673859436224 2316 18-19 -22.340783771781656 2316 20-21 -21.95960195726685 2316 22-23 -21.597017243351733 2316 24-25 -21.469953804523314 2316 26-27 -21.238986352800087 2316 28-29 -21.12276650472944 2316 30-31 -21.2659214495977 2316 32-33 -21.229825793242572 2316 34-35 -20.96279205198134 2316 36-37 -20.41844115454335 2316 38-39 -20.44472340797222 2316 40-41 -20.443569487563735 2316 42-43 -20.368051287119318 2316 44-45 -20.03647996640467 2316 46-47 -19.723442790811262 2316 48-49 -19.582877229914242 2316 50-51 -16.904294278448706 2316 52-53 -19.076169476147932 2316 54-55 -19.987069125264853 2316 56-57 -20.03873999678215 2316 58-59 -19.786702247163163 2316 60-61 -19.547623301324325 2316 62-63 -19.15256810379431 2316 64-65 -18.885484097286025 2316 66-67 -18.60994062070048 2316 68-69 -18.199794071604334 2316 70-71 -17.91410978662351 2316 72-73 -17.562970205564323 2316 74-75 -17.175958994985187 2316 76-77 -16.852663526137466 2316 78-79 -16.540389285827242 2316 80-81 -16.167026887884692 2316 82-83 -15.777501464146443 2316 84-85 -15.44667066494597 2316 86-87 -15.03527352821326 2316 88-89 -14.700123275143852 2316 90-91 -14.263313994821115 2316 92-93 -13.769299998695958 2316 94-95 -13.278368352714748 2316 96-97 -12.680931003905128 2316 98-99 -12.021627097978635 2316 100-101 -10.8856272438233 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 394382.0 3 15763.0 4 69884.0 5 34827.0 6 13074.0 7 13492.0 8 14142.0 9 11948.0 10 10997.0 11 12280.0 12 13583.0 13 14596.0 14 15670.0 15 16978.0 16 18744.0 17 19328.0 18 17782.0 19 17746.0 20 19064.0 21 20392.0 22 22450.0 23 24651.0 24 27001.0 25 29777.0 26 33226.0 27 37678.0 28 42369.0 29 47619.0 30 54924.0 31 63075.0 32 73969.0 33 86945.0 34 102308.0 35 120247.0 36 128297.0 37 100867.0 38 33467.0 39 1213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80252166612116 20.790439868509058 14.25467120149137 23.15236726387841 2 14.72895206037651 20.579253537003094 42.17464411394599 22.517150288674408 3 18.645909386192347 25.846162911691493 30.19367968404169 25.31424801807447 4 11.895025723623512 17.0714944721575 40.006333171746235 31.027146632472753 5 12.417962807356215 38.23225109974955 36.0959564656781 13.253829627216138 6 29.61490413580466 38.875795914992146 18.043865400290603 13.465434548912594 7 26.169072794928034 33.03535899142669 22.91979936200318 17.875768851642096 8 24.200019523961508 37.32095246469244 20.6533049843566 17.825723026989454 9 25.002125254636994 17.03849701992072 21.571680903248964 36.38769682219333 10-11 23.799188808656208 27.61104674125988 29.042966110257463 19.546798339826452 12-13 24.314080804052324 24.891351161521705 29.443087851073972 21.351480183352 14-15 21.552811044893264 26.765939367122726 27.160473800269834 24.520775787714175 16-17 20.853046332196197 29.85360268082409 27.871161446046404 21.422189540933314 18-19 20.6108239357558 28.662879508822304 30.09329909175565 20.632997463666253 20-21 21.550598233037412 28.04791314283045 30.018082267504663 20.38340635662747 22-23 21.037379684647842 27.756158822842103 29.788800564560376 21.417660927949687 24-25 20.920034628671235 27.91750728196039 30.215640110684284 20.946817978684095 26-27 20.79868105302176 28.16389796655892 29.96967288660105 21.067748093818267 28-29 20.727761631632855 28.266287926774314 30.10554910163824 20.900401339954595 30-31 21.132407417275815 28.054967257468512 30.037713671472353 20.774911653783317 32-33 20.99409702071395 28.262931431157888 29.7269003442622 21.016071203865955 34-35 21.017936257087573 28.220499982049823 29.961557313451863 20.80000644741074 36-37 21.089806813692604 27.980223841790735 29.958373000317334 20.971596344199327 38-39 20.948346349859342 28.323640094486663 29.919046861401373 20.80896669425262 40-41 21.080924575494965 28.30936318372907 29.819533983888775 20.7901782568872 42-43 21.056753660353614 28.235216860925856 29.6557650012911 21.05226447742943 44-45 21.04575239758686 28.621973534905386 29.459905863591292 20.872368203916462 46-47 21.045714135506945 28.43890324475617 29.22425363141219 21.291128988324697 48-49 21.09788336065368 28.486572378591845 29.245456273696067 21.170087987058405 50-51 21.3298009846628 28.795725940543864 28.722500290777315 21.151972784016017 52-53 20.90243982947707 28.93906584355859 28.891292164442255 21.267202162522082 54-55 20.931329568021376 28.82775408203867 29.024613761453022 21.216302588486933 56-57 20.960962517353078 29.002054604349837 28.979472466450716 21.057510411846366 58-59 20.894462779611295 28.895892922625592 29.102823615694902 21.106820682068207 60-61 20.96336133375708 28.997021614072327 28.981568412489683 21.05804863968091 62-63 20.824881578459316 28.8247448473084 28.99907706473129 21.351296509500997 64-65 20.687831084431014 28.795479993675016 29.12225883239833 21.39443008949564 66-67 20.79425630031278 28.810641621921242 28.823214766188016 21.57188731157796 68-69 20.7821136389759 28.84749686098263 28.868811625494494 21.50157787454697 70-71 20.833592031109387 28.450332524768974 29.124873594914252 21.591201849207387 72-73 20.845089388770617 28.714557274077112 28.96460501078707 21.4757483263652 74-75 20.697005545490395 28.987148305330123 28.61770172376233 21.698144425417155 76-77 20.74941164925616 28.924077158174683 28.76068871135334 21.56582248121582 78-79 20.74058036967011 28.98275033972782 28.643460427796057 21.633208862806015 80-81 20.742416740755726 29.01121717233559 28.522495567965844 21.723870518942842 82-83 20.92622553855536 28.80339366110025 28.79905293178598 21.47132786855841 84-85 21.053360775616493 28.85088260891948 28.5434525658385 21.552304049625526 86-87 20.704204321591547 28.887956833387214 28.758185125943708 21.649653719077534 88-89 20.967451959553216 28.934950427215156 28.555458143049222 21.542139470182402 90-91 20.873981672659294 28.83295613269814 28.819653519720067 21.4734086749225 92-93 20.877747646232038 28.767594754248815 28.57457064893402 21.780086950585126 94-95 20.689813508729028 28.884597068443906 28.60206510618951 21.823524316637556 96-97 20.70427334472361 28.788774616264394 28.62217549287433 21.88477654613766 98-99 20.53610852180739 28.741884577016798 28.42459266164069 22.297414239535122 100-101 20.800574396918005 28.83708962956267 28.31001439469274 22.05232157882659 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104753.0 1 95131.5 2 63381.5 3 32246.0 4 19933.0 5 15671.5 6 14650.5 7 14861.0 8 15399.0 9 15207.5 10 13923.5 11 11888.0 12 9458.5 13 7287.5 14 5588.0 15 4610.5 16 4342.5 17 4577.5 18 5284.5 19 6179.5 20 7063.0 21 8172.0 22 9317.5 23 10552.0 24 12030.5 25 13542.0 26 15685.0 27 18236.5 28 20570.0 29 23139.0 30 26209.0 31 29215.5 32 32864.0 33 37000.0 34 40685.5 35 43998.0 36 47560.0 37 50719.5 38 51951.0 39 52334.0 40 52576.5 41 52229.0 42 51804.5 43 52235.0 44 52971.0 45 53503.5 46 52713.0 47 51099.0 48 49844.5 49 48480.0 50 45938.5 51 42215.5 52 38452.0 53 34764.5 54 31060.0 55 26724.0 56 22088.5 57 17846.0 58 14478.5 59 11868.0 60 9242.0 61 6630.5 62 4564.0 63 3091.0 64 1997.5 65 1222.5 66 777.5 67 509.5 68 323.0 69 192.5 70 130.5 71 90.5 72 61.5 73 45.5 74 34.0 75 31.0 76 22.5 77 14.0 78 11.5 79 8.5 80 7.0 81 4.0 82 3.0 83 2.0 84 1.5 85 1.0 86 0.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.69293301871286 2 28.623795448403822 3 22.464291783558203 4 28.73790573086577 5 31.76801290426827 6 24.53476936963541 7 23.409323278107596 8 19.522218910101934 9 11.517449456889658 10-11 15.075985301614983 12-13 15.197868232711429 14-15 19.28316121141883 16-17 22.484461667469933 18-19 26.374602661644627 20-21 28.435496766968193 22-23 27.993904460497394 24-25 26.564740034155083 26-27 27.998919072519644 28-29 25.011129652793834 30-31 26.809954562043288 32-33 25.833470306532092 34-35 23.95123568397915 36-37 26.870826380202313 38-39 26.984490919373393 40-41 24.082785672696275 42-43 24.90986234889999 44-45 23.694208958882967 46-47 26.871829302606763 48-49 22.331738872436627 50-51 27.904059328432012 52-53 21.508757462717753 54-55 22.632754888550245 56-57 24.745995971595008 58-59 24.028627862855934 60-61 23.56148889402732 62-63 23.390044880777598 64-65 21.950656217701024 66-67 22.448718627333538 68-69 23.40826463779179 70-71 21.925332426988643 72-73 21.798128435357473 74-75 19.17849511493212 76-77 21.361940766288434 78-79 20.47686174436065 80-81 21.50586013132712 82-83 22.341768096481136 84-85 19.367350975481333 86-87 20.891653735468072 88-89 19.732275435923007 90-91 20.837468066672052 92-93 20.835601516641546 94-95 19.913330788882046 96-97 18.813960679869954 98-99 18.229730520321716 100-101 19.875971929316258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1794755.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.96052687906712 #Duplication Level Percentage of deduplicated Percentage of total 1 86.24736464472812 62.92653166410389 2 9.126895647602096 13.318062304386267 3 2.2697610253744824 4.968088809026816 4 0.8934224887392834 2.607383020161021 5 0.4294692766975026 1.5667152353110825 6 0.2595360831876953 1.1361533624102185 7 0.16081078478066504 0.8212987709803512 8 0.11334561070902992 0.6615804381408363 9 0.07795146573768659 0.5118642010095446 >10 0.3973439166312976 5.052882345379133 >50 0.01675785762807275 0.8223747786012392 >100 0.006946604584184967 0.8355659124759385 >500 1.578374399549763E-4 0.0833751361297933 >1k 1.578374399549763E-4 0.19525213164097313 >5k 0.0 0.0 >10k+ 7.891871997748816E-5 4.492871890242847 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 78029 4.347612905382628 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1939 0.10803703012388878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.571791135837482E-5 0.0 12-13 0.0 0.0 0.0 5.571791135837482E-5 0.0 14-15 0.0 0.0 0.0 8.357686703756223E-5 0.0 16-17 5.571791135837482E-5 0.0 0.0 1.1143582271674965E-4 0.0 18-19 5.571791135837482E-5 0.0 0.0 1.1143582271674965E-4 0.0 20-21 5.571791135837482E-5 0.0 0.0 1.3929477839593705E-4 0.0 22-23 5.571791135837482E-5 0.0 0.0 2.228716454334993E-4 0.0 24-25 5.571791135837482E-5 0.0 0.0 2.228716454334993E-4 0.0 26-27 5.571791135837482E-5 0.0 0.0 2.228716454334993E-4 0.0 28-29 5.571791135837482E-5 0.0 0.0 3.621664238294363E-4 0.0 30-31 5.571791135837482E-5 0.0 0.0 0.001476524650996933 0.0 32-33 5.571791135837482E-5 0.0 0.0 0.004123125440519737 0.0 34-35 1.1143582271674965E-4 0.0 0.0 0.008051238191285162 0.0 36-37 1.1143582271674965E-4 0.0 0.0 0.015238848756515513 0.0 38-39 1.1143582271674965E-4 0.0 0.0 0.02537950862373973 0.0 40-41 1.1143582271674965E-4 2.785895567918741E-5 0.0 0.03643951402837713 0.0 42-43 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.0469144813637516 0.0 44-45 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.06190259951915442 0.0 46-47 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.078785126660742 0.0 48-49 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.09680987098517624 0.0 50-51 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.11753693401049167 0.0 52-53 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.1411613284264426 0.0 54-55 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.16768305423302904 0.0 56-57 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.20100236522533715 0.0 58-59 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.2371911486526016 0.0 60-61 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.2765279940716142 0.0 62-63 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.3158369805349477 0.0 64-65 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.35765327300940797 0.0 66-67 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.4079944059216996 0.0 68-69 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.46864335243529065 0.0 70-71 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.5343069109711354 0.0 72-73 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.6004162127978471 0.0 74-75 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.6705650631980409 0.0 76-77 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.7477065114737109 0.0 78-79 1.1143582271674965E-4 5.571791135837482E-5 0.0 0.834097133034871 0.0 80-81 1.3929477839593705E-4 5.571791135837482E-5 0.0 0.9357544623082259 0.0 82-83 1.6715373407512446E-4 5.571791135837482E-5 0.0 1.0454073118615077 0.0 84-85 1.6715373407512446E-4 5.571791135837482E-5 0.0 1.1527757270490957 0.0 86-87 1.6715373407512446E-4 5.571791135837482E-5 0.0 1.2645179982783166 0.0 88-89 1.6715373407512446E-4 5.571791135837482E-5 0.0 1.392112015288995 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1570 0.0 58.632233 1 GTATCAA 3125 0.0 45.494442 1 ATCAACG 3680 0.0 37.987408 3 TCAACGC 3790 0.0 37.278282 4 CAACGCA 3910 0.0 36.382343 5 TATCAAC 3910 0.0 35.566902 2 AACGCAG 4300 0.0 33.647564 6 GAGTACG 155 6.148184E-10 32.993763 1 ACGCAGA 4760 0.0 30.112534 7 CGCAGAG 4860 0.0 28.825956 8 GTACATG 3675 0.0 26.161587 1 TACATGG 3630 0.0 26.056677 2 GCAGAGT 5425 0.0 25.627644 9 ACATGGG 3620 0.0 25.003836 3 GTGGTAT 865 0.0 24.239925 1 TGGTATC 850 0.0 23.458464 2 GTGTAGC 665 0.0 21.532772 1 GAGTACT 3570 0.0 21.325068 12-13 CAGAGTA 5340 0.0 20.48536 10-11 AGAGTAC 4995 0.0 20.293325 10-11 >>END_MODULE